25 research outputs found
Mucosectomy impairs ileal microcirculation and results in flap contraction after experimental ileocystoplasty
Recycling of bowel content: The importance of the right timing
Introduction: Extracorporeal stool transport (recycling of chyme discharged from the proximal stoma
end to the distal end of a high jejunostomy or ileostomy) is thought to be beneficial in preventing
malabsoprtion, sodium loss, cholestasis and atrophy of the distal intestine until restoration of the
intestinal continuity becomes possible. However little is known about its adverse effects. Our aim was to
investigate the microbiological safety of recycling.
Material and Method: Native samples were taken from the proximal stoma in 5 premature neonates who
underwent an ileostomy or a jejunostomy due to necrotising enterocolitis, for qualitative culture. The
first sample was drawn immediately after the change of the stoma bag, further samples were sent from
the stoma bag at 30, 60, 90, 120, 150, and 180 min later. The samples were inoculated by calibrated
(10 μl) loops onto blood agar (5% sheep blood), eosin–methylene blue agar and anaerobic blood agar,
respectively (Oxoid). The aerobic plates were incubated for 18–20 h at 5% CO 2, whereas the anaerobic
plates were incubated for 24–48 h in an anaerobic chamber (Concept 400). The bacterial strains were
identified to species level by specific biochemical reactions, RapID-ANA II system (Oxoid) and ID32E,
Rapid ID 32 Strep ATB automatic system cards (bioMérieux).
Results: The number of colony forming unit (CFU) of Gram-negative bacteria (mainly E. coli)
exponentially increased after 30 min and reached 105 /ml after 120 min. Gram-positive strains (primarily
E. faecalis) were detected after 60 min and CFU increased to 105 /ml after 120 min. The number of
anaerobic (principally Bacteroides fragilis) CFU started to increase after 120 min. In two cases
coagulase negative Staphylococcus strains were isolated the earliest in the chyme. The average of total
CFU approached 105 /ml after 90 min and exceeded 105 /ml after 120 min. Conclusion: The chyme in the stoma bag is colonized by commensal facultative pathogenic enteral/
colonic as well as skin flora species after 120 min. Recycling of stoma bag content may be dangerous
after 90 mi
Spiral intestinal lengthening and tailoring - first in vivo study
ntroduction: Spiral Intestinal Lengthening and Tailoring (SILT) offers a new opportunity for the
surgical treatment of short bowel syndrome. SILT requires less manipulation on the mesentery than the
Bianchi procedure and does not alter the orientation of the muscle fibers like serial transverse
enteroplasty (STEP). This study reports the first SILT results in a surviving animal model.
Material and Methods: Vietnamese minipigs (n = 6) underwent interposition of a reversed intestinal
segment to produce proximal small bowel dilation. Five weeks later the reversed segment was resected,
and the wall of the dilated intestine was cut spirally at 45°–60° to its longitudinal axis. The bowel was
lengthened longitudinally, and the spiral shaped intestinal wound was sutured. Five weeks later, the
animals were explored, and the lengthened segments were measured. Haematoxylin and eosin,
picrosirius, neuron specific enolase, S-100, C-kit, and immunohistochemistry were performed.
Results: Mean lengthening was 74.8% ± 29.5% and mean tailoring (lumen reduction) was
56.25% ± 18.8%. No instances of necrosis, perforation, suture break down, or peritonitis were observed
in 6/6 animals. Four of six animals recovered uneventfully with viable lengthened segments. Statistical
analysis showed no significant difference in length (p = 0,078) and width (p = 0,182) after 5 weeks. Two
animals developed bowel obstruction due to narrowed lumen, adhesion, and strangulation after 14 and
24 days of surgery. In both animals the lumen was tailored by more than 70% to less than 1.5 cm
diameter. The mucosa and the muscle layers in the operated segment had become hypertrophic, but the
orientation of the circular and longitudinal muscle fibres remained normal after the SILT procedure.
There were no signs of chronic ischemia or collagen accumulation after the SILT. The myenteric and
submucosal plexuses and the Cajal cell network appeared normal. Conclusion: The bowel remained viable macroscopically and microscopically after SILT, such that
SILT may be an alternative or an addition to the present technical repertoire of intestinal lengthening.
However the limitations of tailoring should be kept in mind