The culture of employee learning in South Africa: towards a conceptual framework

The shortage of skills amongst employees in both the private and public sectors in South Africa continues to be a topical issue as exemplified by the continued existence of a list of scarce skills which is published by the Department of Higher Education (DHET). However, the notion that there is a shortage of skills in the country has begun to be challenged with some scholars arguing that the real problem is a jobs shortage attributable to structural inequalities which are a legacy of apartheid and failure by the government post-1994 to address these inequalities. This, we argue, is the reason why unemployment, unemployability and wide workplace inequalities, especially as they affect people from previously disadvantaged groups (mainly women and black employees), persist. We further contend that what is missing from the debates around skills shortage in South Africa and the wider phenomenon to which these debates belong, that is, employee learning, is a holistic conceptualisation of the culture associated with it on the part of the government, employers, workers’ unions and even academia. Conceptualisation of this culture needs to go beyond the government and employer initiatives to the actual process by which employee learning takes place. In other words, it also needs to take into account the employees’ biographies, identities and subjectivities as well as the social interactions which they engage in as they learn in the workplace. We therefore propose a two-tier framework which integrates implications from two theories, that is Human Capital Theory (HCT) and Critical Realism (CR). Implied in HCT is the suggestion that the culture of employee learning is a function of the employer-initiated learning programmes, such as short courses offered by private employee learning service providers, Adult Basic Education and Training (ABET) and block-release programmes run by some institutions of higher learning. The basic aim of these forms of learning would be to increase profitability through improved productivity which itself is a result of employees having been equipped with the requisite skills. Using CR, and Bourdieu’s (1986) idea of habitus, we, however, argue that the final architecture of the culture of employee learning is not linear but a complex and multi-layered product of such factors as the employees’ family and educational backgrounds as well as individual and collective agency in addition to the government and employers’ initiatives such as the afore-mentioned short courses. We also draw on Bernstein’s (1996) notion of learning domains to suggest that attention be paid to employees’ lived experiences which also mediate their responses to the government and employee learning initiatives. This would help with aligning government and organisational employee learning initiatives and strategies to the employees’ individual and collective workplace learning aspirations

TDRSS momentum unload planning

A knowledge-based system is described which monitors TDRSS telemetry for problems in the momentum unload procedure. The system displays TDRSS telemetry and commands in real time via X-windows. The system constructs a momentum unload plan which agrees with the preferences of the attitude control specialists and the momentum growth characteristics of the individual spacecraft. During the execution of the plan, the system monitors the progress of the procedure and watches for unexpected problems

Identification of Novel Pesticides for Use against Glasshouse Invertebrate Pests in UK Tomatoes and Peppers

To inform current and future pesticide availability to glasshouse vegetable growers, the current project trialled more than twenty products, including existing industry standards, against four key pests of glasshouse tomatoes and bell peppers. These included experimental conventional chemical pesticides as well as alternative biopesticide and biorational products based on phytochemicals, microbials and physically-acting substances. The results suggest that certain biopesticide products, particularly botanicals, provide good levels of pest control, with the same being true of experimental conventional chemical pesticides not yet recommended for use against these pests on these crops. Efforts are on-going to ensure that results of the current project translate to industry benefit via new pesticide approvals

TOPO3α Influences Antigenic Variation by Monitoring Expression-Site-Associated VSG Switching in Trypanosoma brucei

Homologous recombination (HR) mediates one of the major mechanisms of trypanosome antigenic variation by placing a different variant surface glycoprotein (VSG) gene under the control of the active expression site (ES). It is believed that the majority of VSG switching events occur by duplicative gene conversion, but only a few DNA repair genes that are central to HR have been assigned a role in this process. Gene conversion events that are associated with crossover are rarely seen in VSG switching, similar to mitotic HR. In other organisms, TOPO3α (Top3 in yeasts), a type IA topoisomerase, is part of a complex that is involved in the suppression of crossovers. We therefore asked whether a related mechanism might suppress VSG recombination. Using a set of reliable recombination and switching assays that could score individual switching mechanisms, we discovered that TOPO3α function is conserved in Trypanosoma brucei and that TOPO3α plays a critical role in antigenic switching. Switching frequency increased 10–40-fold in the absence of TOPO3α and this hyper-switching phenotype required RAD51. Moreover, the preference of 70-bp repeats for VSG recombination was mitigated, while homology regions elsewhere in ES were highly favored, in the absence of TOPO3α. Our data suggest that TOPO3α may remove undesirable recombination intermediates constantly arising between active and silent ESs, thereby balancing ES integrity against VSG recombination

Telomere structure and shortening in telomerase-deficient Trypanosoma brucei

Telomerase consists of a reverse transcriptase (TERT) and an RNA that contains a template for telomere-repeat extension. Telomerase is required to prevent telomere erosion and its activity or lack thereof is important for tumorigenesis and ageing. Telomerase has been identified in numerous organisms but it has not been studied in kinetoplastid protozoa. Trypanosoma brucei, the causative agent of African sleeping sickness, evades the host immune response by frequently changing its variant surface glycoprotein (VSG). The single expressed VSG is transcribed from one of ∼20 subtelomeric ‘Expression Sites’, but the role telomeres might play in regulating VSG transcription and switching is unknown. We identified and sequenced the T.brucei TERT gene. Deleting TERT resulted in progressive telomere shortening of 3–6 bp per generation. In other organisms, the rate of telomere shortening is proportional to the length of the terminal 3′ single-strand overhang. In T.brucei, G-overhangs were undetectable (<30 nt) by in-gel hybridization. The rate of telomere shortening therefore, agrees with the predicted shortening due to the end replication problem, and is consistent with our observation that G-overhangs are short. Trypanosomes whose telomere length can be manipulated provide a new tool to investigate the role of telomeres in antigenic variation

Transverse magnetic tweezers allowing coincident epi-fluorescence microscopy on horizontally extended DNA

Longitudinal magnetic tweezers (L-MT) have seen wide-scale adoption as the tool-of-choice for stretching and twisting a single DNA molecule. They are also used to probe topological changes in DNA as a result of protein binding and enzymatic activity. However, in the longitudinal configuration, the DNA molecule is extended perpendicular to the imaging plane. As a result, it is only possible to infer biological activity from the motion of the tethered superparamagnetic microsphere. Described here is a “transverse” magnetic tweezers (T-MT) geometry featuring simultaneous control of DNA extension and spatially coincident video-rate epi-fluorescence imaging. Unlike in L-MT, DNA tethers in T-MT are extended parallel to the imaging plane between two micron-sized spheres, and importantly protein targets on the DNA can be localised using fluorescent nanoparticles. The T-MT can manipulate a long DNA construct at molecular extensions approaching the contour length defined by B-DNA helical geometry, and the measured entropic elasticity agrees with the worm-like chain model (force < 35 pN). By incorporating a torsionally constrained DNA tether, the T-MT would allow both the relative extension and twist of the tether to be manipulated, while viewing far-red emitting fluorophore-labelled targets. This T-MT design has the potential to enable the study of DNA binding and remodelling processes under conditions of constant force and defined torsional stress