A cross‐lagged twin study of emotional symptoms, social isolation and peer victimisation from early adolescence to emerging adulthood

Background Emotional symptoms, such as anxiety and depressive symptoms, are common during adolescence, often persist over time, and can precede the emergence of severe anxiety and depressive disorders. Studies suggest that a vicious cycle of reciprocal influences between emotional symptoms and interpersonal difficulties may explain why some adolescents suffer from persisting emotional symptoms. However, the role of different types of interpersonal difficulties, such as social isolation and peer victimisation, in these reciprocal associations is still unclear. In addition, the lack of longitudinal twin studies conducted on emotional symptoms during adolescence means that the genetic and environmental contributions to these relationships during adolescence remain unknown. Methods Participants (N = 15,869) from the Twins Early Development Study completed self-reports of emotional symptoms, social isolation and peer victimisation at 12, 16 and 21 years old. A phenotypic cross-lagged model examined reciprocal associations between variables over time, and a genetic extension of this model examined the aetiology of the relationships between variables at each timepoint. Results First, emotional symptoms were reciprocally and independently associated with both social isolation and peer victimisation over time, indicating that different forms of interpersonal difficulties uniquely contributed to emotional symptoms during adolescence and vice versa. Second, early peer victimisation predicted later emotional symptoms via social isolation in mid-adolescence, indicating that social isolation may constitute an intermediate pathway through which peer victimisation predicts longer-term emotional symptoms. Finally, individual differences in emotional symptoms were mostly accounted for by non-shared environmental factors at each timepoint, and both gene–environment and individual-specific environmental mechanisms were involved in the relationships between emotional symptoms and interpersonal difficulties. Conclusions Our study highlights the necessity to intervene early in adolescence to prevent the escalation of emotional symptoms over time and to consider social isolation and peer victimisation as important risk factors for the long-term persistence of emotional symptoms

Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication

Directed evolution is a powerful tool to improve the characteristics of biomolecules. Here we present a protocol for the intracellular evolution of proteins with distinct differences and advantages in comparison with established techniques. These include the ability to select for a particular function from a library of protein variants inside cells, minimizing undesired coevolution and propagation of nonfunctional library members, as well as allowing positive and negative selection logics using basally active promoters. A typical evolution experiment comprises the following stages: (i) preparation of a combinatorial M13 phagemid (PM) library expressing variants of the gene of interest (GOI) and preparation of the Escherichia coli host cells; (ii) multiple rounds of an intracellular selection process toward a desired activity; and (iii) the characterization of the evolved target proteins. The system has been developed for the selection of new orthogonal transcription factors (TFs) but is capable of evolving any gene—or gene circuit function—that can be linked to conditional M13 phage replication. Here we demonstrate our approach using as an example the directed evolution of the bacteriophage λ cI TF against two synthetic bidirectional promoters. The evolved TF variants enable simultaneous activation and repression against their engineered promoters and do not cross-react with the wild-type promoter, thus ensuring orthogonality. This protocol requires no special equipment, allowing synthetic biologists and general users to evolve improved biomolecules within ~7 weeks

A lexicon based approach to classification of ICD10 codes. IMS unipd at CLEF eHealth task 1

International audienc

Les rêves et leur fonctionnement : dans Le mas Théotime de Henri Bosco

論文Article

Middle Mississippian carbonates of the Illinois Basin : a seminar and core workshop, April 23rd and 24th, 1981, Mt. Vernon, Illinois

co-sponsored by the Illinois Geological Society and the Illinois State Geological SurveyOpe

Monstrous things: horror, othering, and the Anthropocene

This article approaches the masses of discarded things washed ashore and roaming waterways as the new monsters of the Anthropocene. It explores the ways in which monstrosity and archaeology intersect, and how the genre of horror simultaneously emerges from and informs the current epoch. As they embark on their post-abandonment journey, things’ immense scale, spread, and refusal to serve as proxies for human narratives result in the impossibility of fully grasping and making sense of them. Combining archaeological approaches and queer theory, this article attempts to get to the heart of the inevitable, complex entanglements between people and monstrous Others

A Profile of Fatherhood Among Young Men: Moving Away from Their Birth Family and Closer to Their Child.

Have things changed all that much in terms of how fatherhood is conceptualized and exercised in daily life? That is the question underlying this article. The author compares the findings of a recent analysis on certain aspects of the lived experiences of young fathers (under 25 years of age) with the results of studies undertaken over the past ten years, and replies in the affirmative. First of all, when considering the representations held of fathers or mothers, most of these young fathers believe that their role is a multi-faceted one, and that it is often identical to that of their spouse. According to young fathers, fatherhood is a dual experience that requires them to be present on a daily basis while also casting their eye on the future. This is an experience that is constructed out of affectionate moments, child-care duties, education in the literal sense, and especially out of shared experiences with their spouse. In addition, they question the degree to which involvement in a career should take precedence over involvement in their child's life. In other words, the former 'competes' with their ability to be present in their child's daily life, which denotes a change from the attitudes of previous generations.Fatherhood, Young Fathers, Representation, Paternal Identity, Qualitative Research

An interactive two-dimensional approach to query aspects rewriting in systematic reviews. IMS unipd at CLEF eHealth task 2

International audienc

El devenir del sujeto y la permanencia del objeto

This article analyzes the construction of a subject who is confronted to the long past of dependency and heteronomy of female individuals.En este artículo se analiza la construcción de un sujeto confrontado a un largo pasado de las mujeres marcado por la dependencia y heteronimia de las personas de sexo femenino