Implementering av kvalitetssystem ved tre bedrifter. Kunnskapsoverføring fra kilde til mottaker

Tema for denne masteroppgaven handler om implementering av et kvalitetssystem ved tre bedrifter på Vestlandet. Kvalitetssystemet som skulle implementeres var utviklet og hadde vært i bruk ved en bedrift i Nord-Norge. Det rettes derfor et fokus på å overføre et kvalitetssytem fr en bedrift i et fiskerikonsern til tre andre bedrifter i smme konsern. Detter at implementeringen var gjennomført ved de tre bedriftene kom det tilbakemelding om variajon i hvordan det nye nystemet ble tatt i bruk. I denne oppgaven er jeg opptatt av hvordan denn overerføringsprosessen forløp og hva som ble utfallet

Applications of geo-referenced underwater photo mosaics in marine biology and archaeology

Author Posting. © Oceanography Society, 2007. This article is posted here by permission of Oceanography Society for personal use, not for redistribution. The definitive version was published in Oceanography 20, 4 (2007): 140-149.In deep water, below the photic zone, still and video imaging of the seabed requires artificial lighting. Light absorption and backscatter caused by typical seawater components, such as dissolved organic matter, plankton, and inorganic particles, often limit the artificially lit area to a few square meters. To obtain high-resolution photographic data of larger seabed areas, a series of images can be compiled into a photo mosaic. Image mosaics are easier to interpret, communicate, and exhibit than video footage or a series of images, because the individual image frames in a photo mosaic are naturally represented in a spatial context

In situ calibration of observatory broadband echosounders

Today, numerous scientific echosounders are used as continuously monitoring systems in ocean observatories. These echosounders are usually calibrated in shallow water, either in laboratory tanks or at random ocean docks before deployments. If the systems are used for quantitative measurements by the observatories, they should be calibrated at the operating depths to consider the environmental effects on the calibration parameters. In this article, a simple in situ calibration method is presented, which was recently applied to one of the nodes of the Norwegian Lofoten-Vesterålen ocean observatory, when the research vessel with dynamic positioning system suspended and moved the calibration sphere between the vessel and the transducer. The calibration results of a 70-kHz split-beam echosounder demonstrate that this method can be applied to the cabled observatories.publishedVersio

Actinomycetes from Sediments in the Trondheim Fjord, Norway: Diversity and Biological Activity

The marine environment represents a largely untapped source for isolation of new microorganisms with potential to produce biologically active secondary metabolites. Among such microorganisms, Gram-positive actinomycete bacteria are of special interest, since they are known to produce chemically diverse compounds with a wide range of biological activities. We have set out to isolate and characterize actinomycete bacteria from the sediments in one of the largest Norwegian fjords, the Trondheim fjord, with respect to diversity and antibiotic-producing potential. Approximately 3,200 actinomycete bacteria were isolated using four different agar media from the sediment samples collected at different locations and depths (4.5 to 450 m). Grouping of the isolates first according to the morphology followed by characterization of isolates chosen as group representatives by molecular taxonomy revealed that Micromonospora was the dominating actinomycete genus isolated from the sediments. The deep water sediments contained a higher relative amount of Micromonospora compared to the shallow water samples. Nine percent of the isolates clearly required sea water for normal growth, suggesting that these strains represent obligate marine organisms. Extensive screening of the extracts from all collected isolates for antibacterial and antifungal activities revealed strong antibiotic-producing potential among them. The latter implies that actinomycetes from marine sediments in Norwegian fjords can be potential sources for the discovery of novel anti-infective agents

Molecular phylogeny of Allodia (Diptera: Mycetophilidae) constructed using genome skimming

In this study, we use low-coverage shotgun sequencing of genomic DNA, commonly referred to as genome skimming, to investigate the phylogenetic relationships of the fungus gnat genus Allodia Winnertz (Mycetophilidae, Mycetophilinae, Exechiini). Nineteen specimens, representing 16 in-group and three outgroup taxa, were successfully sequenced and molecular markers of both mitochondrial and nuclear origin were retrieved. The phylogenetic analyses of 13 protein-coding mitochondrial genes, two ribosomal mitochondrial genes and the nuclear ribosomal 18S and 28S strongly support the monophyly of its two subgenera Allodia s.s. and Brachycampta. Complete mitochondrial genomes of 15 species were assembled, which enables further comparisons with the mitochondrial genomes of other Diptera. Overall, the methodology used in this study proved successful and promising for other dipteran groups. In addition to the phylogenetic reconstruction, the morphological characters previously used to separate the two subgenera were evaluated and re-examined. Together with the composite structure of the male genitalia, we consider details of the scutal bristles appropriate for separating the two groups. Based on the achieved results, we reinstate Brachycampta stat. rev. as a separate genus

CRIMAC - Annual Report 2020

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Diatom Biogeography From the Labrador Sea Revealed Through a Trait-Based Approach

Diatoms are a keystone algal group, with diverse cell morphology and a global distribution. The biogeography of morphological, functional, and life-history traits of marine diatoms were investigated in Arctic and Atlantic waters of the Labrador Sea during the spring bloom (2013-2014). In this study, trait-based analysis using community-weighted means showed that low temperatures (< 0°C) in Arctic waters correlated positively with diatom species that have traits such as low temperature optimum growth and the ability to produced ice-binding proteins, highlighting their sea ice origin. High silicate concentrations in Arctic waters, as well as sea ice cover and shallow bathymetry, favoured diatom species that were heavily silicified, colonial and capable of producing resting spores, suggesting that these are important traits for this community. In Atlantic waters, diatom species with large surface area to volume ratios were dominant in deep mixed layers, whilst low silicate to nitrate ratios correlated positively with weakly silicified species. Sharp cell projections, such as processes or spines, were positively correlated with water-column stratification, indicating that these traits promote positive buoyancy for diatom cells. Our trait-based analysis directly links cell morphology and physiology with diatom species distribution, allowing new insights on how this method can potentially be applied to explain ecophysiology and shifting biogeographical distributions in a warming climate

Phytoplankton community succession and dynamics using optical approaches

The phytoplankton in coastal regions are responding to constant environmental changes, thus the use of proxies derived from in situ frequent time-series observations and validated from traditional microscopic or pigment methods can be a solution for detecting rapid responses of community dynamics and succession. In this study, we combined in situ high-frequency (every 30 min from May to September 2017) optical and hydrographic data from a moored buoy and weekly discrete samplings to track phytoplankton community dynamics and succession in Mausund Bank, a highly productive region of the coast of Norway. Three hydrographic regimes were observed: mixing period (MP) in spring, onset of stratification (transient period, TP) in summer and a stratified period (SP) in fall, with occasional strong winds that disrupted the surface stratification in the beginning of September. A bloom dominated by the diatom Skeletonema costatum was observed in the MP due to intense mixing and nutrient availability, while flagellates prevailed in nutrient-poor waters during the TP, followed by a bloom dominated by rhizosolenid diatoms (Proboscia alata and Guinardia delicatula), when stratification peaked. A mixed assemblage of diatoms (e.g. Pseudo-nitzschia), coccolithophores and dinoflagellates occurred during the SP, as strong winds reintroduced nutrients to surface waters. Through pigment (chemotaxonomy) and microscopic observations, we tested, for the first time in a coastal region, whether an ‘optical community index’ derived from in situ measurements of chlorophyll a fluorescence (Fchla) and optical particulate backscattering (bbp) is suitable to differentiate between diatom versus flagellate dominance. We found a negative relationship between Fchla:bbp and diatom:flagellate, contrary to previous observations, possibly because of the influence of non-algal contribution (e.g. zooplankton, fecal pellets and detritus) to the bbp pool in highly productive systems. This finding suggests that such relationship is not universal and that other parameters are needed to refine the optical community index in coastal regions

Interviews with Community Healthcare Registered Nurses in Norway: Examination Practices and Clinical Evaluation Processes

Abstract Aim This study describes the examination practices and clinical evaluation processes that Registered Nurses in Norway perform in the community healthcare sector. Design A qualitative exploratory design. Methods Twenty interviews were conducted with Registered Nurses employed in the community healthcare sector in Norway. The data were analysed using a thematic analysis. Results We found four major themes: (1) evaluations are embedded in nurses’ daily work, (2) significance of a Registered Nurse's clinical competency, (3) different tasks require various roles and (4) access to resources and equipment. Registered Nurses possess several skills in a range of different examination techniques and clinical evaluation processes in the community healthcare sector. They perform complex assessments in their daily work and must rely on other healthcare professionals, facilities and equipment to provide high-quality care. Ongoing education and training will enable Registered Nurses to complete accurate assessments in their community healthcare practice.publishedVersio