Spatio-temporal analysis of Chlamydia trachomatis in Newfoundland and Labrador and an exploratory analysis into employment traits as risk factors

Sexually transmitted infections are an important health concern in Canadian society due to their negative impact on sexual and reproductive health, as well as their social and fiscal ramifications. Chlamydia trachomatis is the most common reported sexually transmitted infection in Canada and has been consistently on the rise since 1997. The province of Newfoundland and Labrador routinely publishes case counts of infectious diseases, including chlamydia, but there is minimal examination into age, sex, the regional distributions of cases, the etiology of these infections, or what populations are most at risk. There is also little information on the role of migratory employment in infectious disease risk in Canada. The spatial and temporal distribution of chlamydia in Newfoundland and Labrador between 2007 and 2013 was examined, in addition to an exploration into migratory employment as a contributory cause. Case data were obtained from the Regional Health Authorities and population information from Statistics Canada. Differences in incidence rates for age, sex, and geography were evaluated over time. A linear increase in incidence occurred over the study period. Distinct areas of high incidence were observed and spatial clusters were identified in northern Labrador and St. John’s. Using the 2006 Census of Population and 2011 National Household Survey, associations between work-related commuting demographics and chlamydia incidence were found in the province. Understanding the distribution of chlamydia as well as the behaviours of migratory workers can help direct health policy to prevent chlamydial and other infections from occurring, and improve overall sexual health practices of Canadians

Ion flow in a zeolitic imidazolate framework results in ionic diode phenomena

Ionic transport (for applications in nanofluidics or membranes) and “ionic diode” phenomena in a zeolitic imidazolate framework (ZIF-8) are investigated by directly growing the framework from aqueous Zn2+ and 2-methylimidazole as an “asymmetric plug” into a 20 ?m diameter pore in a ca. 6 ?m thin poly-ethylene-terephthalate (PET) film

WORKSHOP PEMANFAATAN CLOUD STORAGE ONLINE DI KALANGAN PELAJAR

Dengan kemajuan teknologi di berbagai bidang, seperti bidang Pendidikan, teknologi telah menjadi media pembelajaran yang memiliki posisi sentral dalam proses belajar mengajar dan bukan hanya sebagai alat bantu saja, tetapi juga sebagai alat praktek dalam proses pelatihan. Sebagai salah satu bentuk pembelajaran berbasis teknologi, yaitu berbasis multimedia presentasi yang digunakan untuk membuat dan menggabungkan teks, grafik, audio, gambar, serta video secara bersamaan dalam media penyimpanan. Microsoft Office dan storage online merupakan salah satu perangkat lunak yang dikemas lengkap dalam bentuk multitasking yang dinamis serta menarik.Berdasarkan hal tersebut kegiatan ini bertujuan untuk melakukan Workshop Pemanfaatan Cloud Storage Online di Kalangan Pelajar yang diadakan di SMP Parigi. Kegiatan ini dilakukan dengan metode penelitian deskriftif, dengan Teknik pengumpulan data melalui wawancara, observasi, dan studi dokumentasi.Sehingga disimpulkan bahwa kegiatan ini dapat meningkatkan kemampuan siswa dalam menggunakan program Microsoft Office dan storage online sebagai media pembelajaran

COVID 19:Seroprevalence and vaccine responses in UK dental care professionals

Dental care professionals (DCPs) are thought to be at enhanced risk of occupational exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, robust data to support this from large-scale seroepidemiological studies are lacking. We report a longitudinal seroprevalence analysis of antibodies to SARS-CoV-2 spike glycoprotein, with baseline sampling prior to large-scale practice reopening in July 2020 and follow-up postimplementation of new public health guidance on infection prevention control (IPC) and enhanced personal protective equipment (PPE). In total, 1,507 West Midlands DCPs were recruited into this study in June 2020. Baseline seroprevalence was determined using a combined IgGAM enzyme-linked immunosorbent assay and the cohort followed longitudinally for 6 mo until January/February 2021 through the second wave of the coronavirus disease 2019 pandemic in the United Kingdom and vaccination commencement. Baseline seroprevalence was 16.3%, compared to estimates in the regional population of 6% to 7%. Seropositivity was retained in over 70% of participants at 3- and 6-mo follow-up and conferred a 75% reduced risk of infection. Nonwhite ethnicity and living in areas of greater deprivation were associated with increased baseline seroprevalence. During follow-up, no polymerase chain reaction–proven infections occurred in individuals with a baseline anti–SARS-CoV-2 IgG level greater than 147.6 IU/ml with respect to the World Health Organization international standard 20-136. After vaccination, antibody responses were more rapid and of higher magnitude in those individuals who were seropositive at baseline. Natural infection with SARS-CoV-2 prior to enhanced PPE was significantly higher in DCPs than the regional population. Natural infection leads to a serological response that remains detectable in over 70% of individuals 6 mo after initial sampling and 9 mo from the peak of the first wave of the pandemic. This response is associated with protection from future infection. Even if serological responses wane, a single dose of the Pfizer-BioNTech 162b vaccine is associated with an antibody response indicative of immunological memory

MicroRNA-210 Regulates Mitochondrial Free Radical Response to Hypoxia and Krebs Cycle in Cancer Cells by Targeting Iron Sulfur Cluster Protein ISCU

BACKGROUND: Hypoxia in cancers results in the upregulation of hypoxia inducible factor 1 (HIF-1) and a microRNA, hsa-miR-210 (miR-210) which is associated with a poor prognosis. METHODS AND FINDINGS: In human cancer cell lines and tumours, we found that miR-210 targets the mitochondrial iron sulfur scaffold protein ISCU, required for assembly of iron-sulfur clusters, cofactors for key enzymes involved in the Krebs cycle, electron transport, and iron metabolism. Down regulation of ISCU was the major cause of induction of reactive oxygen species (ROS) in hypoxia. ISCU suppression reduced mitochondrial complex 1 activity and aconitase activity, caused a shift to glycolysis in normoxia and enhanced cell survival. Cancers with low ISCU had a worse prognosis. CONCLUSIONS: Induction of these major hallmarks of cancer show that a single microRNA, miR-210, mediates a new mechanism of adaptation to hypoxia, by regulating mitochondrial function via iron-sulfur cluster metabolism and free radical generation

O novo Museu de Arqueologia e Etnografia

Treatment personalisation remains an unmet need in oropharynx cancer (OPC). We aimed to determine whether gene expression signatures improved upon clinico-pathological predictors of outcome in OPC. The clinico-pathological predictors, AJCC version 7 (AJCC 7), AJCC 8, and a clinical algorithm, were assessed in 4 public series of OPC (n = 235). Literature review identified 16 mRNA gene expression signatures of radiosensitivity, HPV status, tumour hypoxia, and microsatellite instability. We quality tested signatures using a novel sigQC methodology, and added signatures to clinico-pathological variables as predictors of survival, in univariate and multivariate analyses. AJCC 7 Stage was not predictive of recurrence-free survival (RFS) or overall survival (OS). AJCC 8 significantly predicted RFS and OS. Gene signature quality was highly variable. Among HPV-positive cases, signatures for radiosensitivity, hypoxia, and microsatellite instability revealed significant underlying inter-tumour biological heterogeneity, but did not show prognostic significance when adjusted for clinical covariates. Surprisingly, among HPV-negative cases, a gene signature for HPV status was predictive of survival, even after adjustment for clinical covariates. Across the whole series, several gene signatures representing HPV and microsatellite instability remained significant in multivariate analysis. However, quality control and independent validation remain to be performed to add prognostic information above recently improved clinico-pathological variables

Cytotoxic T Lymphocyte Therapy for Epstein-Barr Virus+ Hodgkin's Disease

Epstein Barr virus (EBV)+ Hodgkin's disease (HD) expresses clearly identified tumor antigens derived from the virus and could, in principle, be a target for adoptive immunotherapy with viral antigen–specific T cells. However, like most tumor-associated antigens in immunocompetent hosts, these potential targets are only weakly immunogenic, consisting primarily of the latent membrane protein (LMP)1 and LMP2 antigens. Moreover, Hodgkin tumors possess a range of tumor evasion strategies. Therefore, the likely value of immunotherapy with EBV-specific cytotoxic effector cells has been questioned. We have now used a combination of gene marking, tetramer, and functional analyses to track the fate and assess the activity of EBV cytotoxic T lymphocyte (CTL) lines administered to 14 patients treated for relapsed EBV+ HD. Gene marking studies showed that infused effector cells could further expand by several logs in vivo, contribute to the memory pool (persisting up to 12 mo), and traffic to tumor sites. Tetramer and functional analyses showed that T cells reactive with the tumor-associated antigen LMP2 were present in the infused lines, expanded in peripheral blood after infusion, and also entered tumor. Viral load decreased, demonstrating the biologic activity of the infused CTLs. Clinically, EBV CTLs were well tolerated, could control type B symptoms (fever, night sweats, and weight loss), and had antitumor activity. After CTL infusion, five patients were in complete remission at up to 40 mo, two of whom had clearly measurable tumor at the time of treatment. One additional patient had a partial response, and five had stable disease. The performance and fate of these human tumor antigen–specific T cells in vivo suggests that they might be of value for the treatment of EBV+ Hodgkin lymphoma