Licensed Integrated Examination "Step 1. Dentology" in the Bogomolets National Medical University as Education Quality Control Indicator: Inter-dissiplanal Integration. Monitoring Aspect

The study considers pedagogical means of students' readiness to the licensed integrated exam “Step 1. Stomatology”, used at higher medical educational institutions. The results of licensed integrated exam “Step 1. Stomatology” of students, taught in the official language, for 2015–2017 were analyzed. The disciplines, most complicated for students, were determined by subtests, based on the results of the independent state exam. The dynamics and successfulness of total modular controls in biological chemistry by students of the stomatological department of the National medical university, named after O.O. Bogomolets, were studied. The main causes of their academic results on biological chemistry were determined. The comparative analysis of stomatological students' results in the discipline “Biological chemistry” and their successfulness in the same discipline within the licensed integrated exam “Step 1. Stomatology” was mad

New Possibilities of Use of Oat Raw Materials for Investigation of Biologically Active Components in Therapy of Experimental Diabetes

Aim of the work is to suggest the technology of aqua dynamic extraction of bioactive substances from oat straw. The amount of the extract and the concentration of extracted substances depend on the duration of extraction process. The composition of the investigated phytocomplex is established by nuclear magnetic resonance. Fatty acids composition of the phytocomplex of the oat straw extract was studied by gas chromatography. The possibility of using a phytocomplex from an oat straw extract is shown in the study of the metabolism of myocardium lipids in experimental animals with a diabetes model.Methods of research: to optimise the process of aqueous extraction of the oat straw that in prospect, with the help of dynamic character and increased temperatures, can prevail obvious disadvantages of technology offered before; in order to evaluate the differences in the content of the mixtures of the substances, received from the primary extract was noted observation Н1–NMR spectra in D2O; was performed gas chromatographic analysis of the fatty acids spectrum in the studied phytocomplex and in the lipids of the white blood cells of the myocardium; modeling of diabetes was performed by intraperitoneal streptozotocin injection at dose of 55 mg per kilo of body weight of rats during 6 weeks.Results: was developed more economically expedient approach to obtain a phytocomplex by extracting oat straw with full preservation of biologically active substances. Fatty acid composition of the phytocomplex of the oat straw extract was studied by gas chromatography. Composition of the phytocomplex of the oat straw extract was studied by nuclear magnetic resonanceConclusions: The possibility of using a phytocomplex from an oat straw extract in the study of the metabolism of myocardium lipids in experimental animals with a model of diabetes is show

The role of pre-existing cross-reactive central memory CD4 T-cells in vaccination with previously unseen influenza strains.

Influenza vaccination is a common approach to prevent seasonal and pandemic influenza. Pre-existing antibodies against close viral strains might impair antibody formation against previously unseen strains-a process called original antigenic sin. The role of this pre-existing cellular immunity in this process is, despite some hints from animal models, not clear. Here, we analyzed cellular and humoral immunity in healthy individuals before and after vaccination with seasonal influenza vaccine. Based on influenza-specific hemagglutination inhibiting (HI) titers, vaccinees were grouped into HI-negative and -positive cohorts followed by in-depth cytometric and TCR repertoire analysis. Both serological groups revealed cross-reactive T-cell memory to the vaccine strains at baseline that gave rise to the majority of vaccine-specific T-cells post vaccination. On the contrary, very limited number of vaccine-specific T-cell clones was recruited from the naive pool. Furthermore, baseline quantity of vaccine-specific central memory helper T-cells and clonotype richness of this population directly correlated with the vaccination efficacy. Our findings suggest that the deliberate recruitment of pre-existing cross-reactive cellular memory might help to improve vaccination outcome