A Blind Comparative Study of Focused Wave Interactions with a Fixed FPSO-like Structure (CCP-WSI Blind Test Series 1)

Results from Blind Test Series 1, part of the Collaborative ComputationalProject in Wave Structure Interaction (CCP-WSI), are presented. Partici-pants, with a range of numerical methods, simulate blindly the interactionbetween a fixed structure and focused waves ranging in steepness and di-rection. Numerical results are compared against corresponding physicaldata. The predictive capability of each method is assessed based on pres-sure and run-up measurements. In general, all methods perform well inthe cases considered, however, there is notable variation in the results(even between similar methods). Recommendations are made for appro-priate considerations and analysis in future comparative studies

Formations p\ue9ridermiques dans le p\ue9tiole du cotyl\ue9don de quelques Palmiers

Volume: 54Start Page: 206End Page: 21

Un cas de polyembryonie chez le Musa Ensete

Volume: 52Start Page: 277End Page: 27

Utilisation de la xylane le Xylaria Hypoxylon L

Volume: 57Start Page: 127End Page: 13

Un cas de polyembryonie chez le Triglochin palustre L. et une germination anormale de l\u27 Arisarum vulgare Targ.-Tozz

Volume: 55Start Page: 164End Page: 16

Impact of achieving bone sterilization on bone architecture and bone marrow, in an experimental rabbit model of osteomyelitis caused by carbapenemase-producing Enterobacterales

International audienceObjectives - Natural history and treatment of bone infections caused by carbapenemase-producing Enterobacterales (CPE) are poorly defined. We evaluated the effect of treatment on the progression of subacute osteomyelitis in a rabbit model. Methods - Two isolates were used: a KPC-producing Klebsiella pneumoniae and an Escherichia coli harbouring bla and bla inserts, both susceptible to gentamicin, colistin, fosfomycin, and ceftazidime-avibactam. Osteomyelitis was induced in rabbits by tibial injection of 2 × 10 colony-forming units/mL. Antibiotics were started 14 d later, for 7 d, in 6 groups of 12 rabbits. Three days after treatment completion (D24), rabbits were euthanised and bones were cultured. Bone marrow and bone architecture macroscopic changes were evaluated through analysis of pictures by investigators unaware of the rabbit treatment group and microbiological outcome, using scales ranging from 0 (normal) to 3 (severe lesions) depending on modifications. Results - Bone marrow modifications induced by local infection were similar between prematurely deceased animals and non-sterilised animals (P = 0.14) but differed significantly from animals that achieved bone sterilisation after treatment (P = 0.04). Conversely, when comparing bone deformity, rabbits who died early (n = 13) had similar bone architecture as those achieving bone sterilisation (P = 0.12), as opposed to those not sterilised after treatment (P = 0.04). After a multivariate logistic regression, bone marrow scale ≤2 was associated with bone sterilisation (P < 0.001), and bone architecture scale ≤2 was associated with bone sterilisation (adjusted odds ratio = 2.7; 95% confidence interval 1.14-6.37) and KPC infection (adjusted odds ratio = 5.1; 95% confidence interval 2.17-12.13). Conclusion - Effective antibacterial treatment reduces bone architecture distortion and bone marrow changes. These variables may be used as proxy for bone sterilisation

Efficacy of generic meropenem products in combination with colistin in carbapenemase-producing Klebsiella pneumoniae experimental osteomyelitis

International audienceGuidelines for the management of carbapenemase-producing Enterobacterales (CPE) infections recommend a combination of two active agents, including meropenem if the minimum inhibitory concentration (MIC) is ≤8 mg/L. The therapeutic equivalence of meropenem generics has been challenged. We compared the bactericidal activity of meropenem innovator (AstraZeneca) and four generic products (Actavis, Kabi, Mylan and Panpharma), both in vitro and in vivo, in association with colistin. In vitro time-kill studies were performed at 4 × MIC. An experimental model of KPC-producing Klebsiella pneumoniae osteomyelitis was induced in rabbits by tibial injection of a sclerosing agent followed by 2 × 10 CFU of K. pneumoniae KPC-99YC (meropenem MIC = 4 mg/L; colistin MIC = 1 mg/L). At 14 days after inoculation, treatment for 7 days started in seven groups of ≥10 rabbits, including a control group, a colistin group, and one group for each meropenem product (i.e. the innovator and four generics), in combination with colistin. In vitro, meropenem + colistin was bactericidal with no viable bacteria after 6 h, and this effect was similar with all meropenem products. In the osteomyelitis model, there was no significant difference between meropenem generics and the innovator when combined with colistin. Colistin-resistant strains were detected after treatment with colistin + meropenem innovator (n = 3) and generics (n = 3). The efficacy of four meropenem generics did not differ from the innovator in vitro and in an experimental rabbit model of KPC-producing K. pneumoniae osteomyelitis in terms of bactericidal activity and the emergence of resistance