Non-Markoffian effects of a simple nonlinear bath

We analyze a model of a nonlinear bath consisting of a single two-level system coupled to a linear bath (a classical noise force in the limit considered here). This allows us to study the effects of a nonlinear, non-Markoffian bath in a particularly simple situation. We analyze the effects of this bath onto the dynamics of a spin by calculating the decay of the equilibrium correlator of the spin's z-component. The exact results are compared with those obtained using three commonly used approximations: a Markoffian master equation for the spin dynamics, a weak-coupling approximation, and the substitution of a linear bath for the original nonlinear bath.Comment: 7 pages, 6 figure

A method for determining gas-hydrate or free-gas saturation of porous media from seismic measurements

The occurrence of gas hydrate or free gas in a porous medium changes the medium’s elastic properties. Explicit formulas for gas-hydrate or free-gas saturation of pore space on the basis of the Frenkel-Gassmann equations describe the elastic moduli and seismic velocities of a porous medium for low frequencies. A key assumption of the model is that either gas hydrate or free gas is present in the pore space in addition to water. Under this assumption, the method uses measured P- and S-wave velocities and bulk density along with estimates of the moduli and densities of the solid and fluid phases present to determine whether gas or hydrate is present. The method then determines the saturation level of either the gas or the hydrate. I apply the method to published velocity and density data from seismic studies at the antarctic Shetland margin and at the Storegga slide, offshore Norway, and to borehole log and core data from Ocean Drilling Program (ODP) Leg 164 at Blake Ridge, offshore South Carolina. A sensitivity analysis reveals that the standard deviations of the gas-hydrate and free-gas saturations reach 30%–70% of the saturations if the standard deviations of the P- and S-wave velocities and of the bulk density are 50m∕s ..

Probing Ionization Energies for Trace Gas Identification: The Micro Photo Electron Ionization Detector (PEID)

Micro gas sensors detect the presence of substances, but can hardly identify them. We developed a novel approach of probing referenceable ionization energies. It extends the photoionization principle towards tunable energies via replacement of photons by accelerated photo electrons. The device comprises UV-LED illumination, an atmospherically stable photoelectron emission layer with a nano-vacuum electronics accelerator realized in thin film technology and charged particle measurement. A voltage variation at the accelerator provides electrons of tunable energies. We were able to prove that variable electron energies can be used for substance detection. The resulting system reaches ambient conditions operability. The actual limitations and challenges are discussed

Preventing Farnesylation of the Dynein Adaptor Spindly Contributes to the Mitotic Defects Caused by Farnesyltransferase Inhibitors

The clinical interest in farnesyltransferase inhibitors (FTIs) makes it important to understand how these compounds affect cellular processes involving farnesylated proteins. Mitotic abnormalities observed after treatment with FTIs have so far been attributed to defects in the farnesylation of the outer kinetochore proteins CENP-E and CENP-F, which are involved in chromosome congression and spindle assembly checkpoint signaling. Here we identify the cytoplasmic dynein adaptor Spindly as an additional component of the outer kinetochore that is modified by farnesyltransferase (FTase). We show that farnesylation of Spindly is essential for its localization, and thus for the proper localization of dynein and its cofactor dynactin, to prometaphase kinetochores and that Spindly kinetochore recruitment is more severely affected by FTase inhibition than kinetochore recruitment of CENP-E and CENP-F. Molecular replacement experiments show that both Spindly and CENP-E farnesylation are required for efficient chromosome congression. The identification of Spindly as a new mitotic substrate of FTase provides insight into the causes of the mitotic phenotypes observed with FTase inhibitors

Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle

The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres and the central spindle in mitosis. Here, we describe Borealin, a novel member of the complex. Approximately half of Aurora B in mitotic cells is complexed with INCENP, Borealin, and Survivin; and Borealin binds Survivin and INCENP in vitro. A second complex contains Aurora B and INCENP, but no Borealin or Survivin. Depletion of Borealin by RNA interference delays mitotic progression and results in kinetochore–spindle misattachments and an increase in bipolar spindles associated with ectopic asters. The extra poles, which apparently form after chromosomes achieve a bipolar orientation, severely disrupt the partitioning of chromosomes in anaphase. Borealin depletion has little effect on histone H3 serine10 phosphorylation. These results implicate the chromosomal passenger holocomplex in the maintenance of spindle integrity and suggest that histone H3 serine10 phosphorylation is performed by an Aurora B–INCENP subcomplex