59 research outputs found
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Modeling the resolution of inexpensive, novel non-seismic geophysical monitoring tools to monitor CO2 injection into coal beds
A sensitivity study of gravity and electromagnetic (EM) techniques, and amplitude vs. angle (AVA) analysis for CO{sub 2} movement in coal beds was based on the SECARB pilot test planned in the Black Warrior basin in Alabama. In the area of interest, coalbed methane is produced mainly from the Black Creek, Mary Lee, and Pratt coal zones at depths between 400 and 700 m and approximately 3 m thick on average. The permeability of coal in the Black Warrior basin decreases exponentially with depth as overburden stress increases. The permeability of the top layer is 100 mD, while the permeability of the deepest layer is around 1 mD. The pilot field test will include injecting a total of 1000 tons of CO{sub 2} into these three coal zones ({approx}300 tons to each zone). The density, sonic and resistivity well-logs from a deep disposal well a couple of miles from the pilot test site were used to create background (pre-injection) models. Our laboratory measurements of seismic velocity and electrical resistivity as a function of CO{sub 2} saturation on coal core samples were used to provide a link between the coalbed CO{sub 2} flow simulation models and the geophysical models. The sensitivity studies showed that while the response to the 300 tons of CO{sub 2} injected into a single layer wouldn't produce measurable surface response for either gravity or EM, the response due to an industrial-size injection would produce measurable surface signal for both techniques. Gravity inversion results illustrated that, provided we can collect high-quality gravity data in the field and we have some a priori information about the depth of the reservoir, we can recover the spatial location of CO{sub 2} plume correctly, although with the smoothing constraint of the inversion, the area was slightly overestimated, resulting in an underestimated value of density change. AVA analysis showed that by inverting seismic and EM data jointly, much better estimates of CO{sub 2} saturation can be obtained, especially in the third injection zone, where seismic AVA data fail to detect the high CO{sub 2} saturation. Analysis of spatial resolution and detectability limits show that gravity and EM measurements could, under certain circumstances, be used as a lower-cost alternative to seismic measurements
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UXO detection and identification based on intrinsic target polarizabilities: A case history
Electromagnetic induction data parameterized in time dependent object intrinsic polarizabilities allow discrimination of unexploded ordnance (UXO) from false targets (scrap metal). Data from a cart-mounted system designed for discrimination of UXO with 20 mm to 155 mm diameters are used. Discrimination of UXO from irregular scrap metal is based on the principal dipole polarizabilities of a target. A near-intact UXO displays a single major polarizability coincident with the long axis of the object and two equal smaller transverse polarizabilities, whereas metal scraps have distinct polarizability signatures that rarely mimic those of elongated symmetric bodies. Based on a training data set of known targets, object identification was made by estimating the probability that an object is a single UXO. Our test survey took place on a military base where both 4.2-inch mortar shells and scrap metal were present. The results show that we detected and discriminated correctly all 4.2-inch mortars, and in that process we added 7%, and 17%, respectively, of dry holes (digging scrap) to the total number of excavations in two different survey modes. We also demonstrated a mode of operation that might be more cost effective than the current practice
EIF2S3 Mutations Associated with Severe X-Linked Intellectual Disability Syndrome MEHMO
Impairment of translation initiation and its regulation within the integrated stress response (ISR) and related unfolded-protein response has been identified as a cause of several multisystemic syndromes. Here, we link MEHMO syndrome, whose genetic etiology was unknown, to this group of disorders. MEHMO is a rare X-linked syndrome characterized by profound intellectual disability, epilepsy, hypogonadism and hypogenitalism, microcephaly, and obesity. We have identified a C-terminal frameshift mutation (Ile465Serfs) in the EIF2S3 gene in three families with MEHMO syndrome and a novel maternally inherited missense EIF2S3 variant (c.324T>A; p.Ser108Arg) in another male patient with less severe clinical symptoms. The EIF2S3 gene encodes the gamma subunit of eukaryotic translation initiation factor 2 (eIF2), crucial for initiation of protein synthesis and regulation of the ISR. Studies in patient fibroblasts confirm increased ISR activation due to the Ile465Serfs mutation and functional assays in yeast demonstrate that the Ile465Serfs mutation impairs eIF2gamma function to a greater extent than tested missense mutations, consistent with the more severe clinical phenotype of the Ile465Serfs male mutation carriers. Thus, we propose that more severe EIF2S3 mutations cause the full MEHMO phenotype, while less deleterious mutations cause a milder form of the syndrome with only a subset of the symptoms
Identification of a Novel Ī²-Cell Glucokinase (GCK) Promoter Mutation (ā71G>C) That Modulates GCK Gene Expression Through Loss of Allele-Specific Sp1 Binding Causing Mild Fasting Hyperglycemia in Humans
OBJECTIVE: Inactivating mutations in glucokinase (GCK) cause mild fasting hyperglycemia. Identification of a GCK mutation has implications for treatment and prognosis; therefore, it is important to identify these individuals. A significant number of patients have a phenotype suggesting a defect in glucokinase but no abnormality of GCK. We hypothesized that the GCK beta-cell promoter region, which currently is not routinely screened, could contain pathogenic mutations; therefore, we sequenced this region in 60 such probands. RESEARCH DESIGN AND METHODS: The beta-cell GCK promoter was sequenced in patient DNA. The effect of the identified novel mutation on GCK promoter activity was assessed using a luciferase reporter gene expression system. Electrophoretic mobility shift assays (EMSAs) were used to determine the impact of the mutation on Sp1 binding. RESULTS: A novel -71G>C mutation was identified in a nonconserved region of the human promoter sequence in six apparently unrelated probands. Family testing established cosegregation with fasting hyperglycemia (> or = 5.5 mmol/l) in 39 affected individuals. Haplotype analysis in the U.K. family and four of the Slovakian families demonstrated that the mutation had arisen independently. The mutation maps to a potential transcriptional activator binding site for Sp1. Reporter assays demonstrated that the mutation reduces promoter activity by up to fourfold. EMSAs demonstrated a dramatic reduction in Sp1 binding to the promoter sequence corresponding to the mutant allele. CONCLUSIONS: A novel beta-cell GCK promoter mutation was identified that significantly reduces gene expression in vitro through loss of regulation by Sp1. To ensure correct diagnosis of potential GCK-MODY (maturity-onset diabetes of the young) cases, analysis of the beta-cell GCK promoter should be included
Mutations in HNF1A Result in Marked Alterations of Plasma Glycan Profile
A recent genome-wide association study identified hepatocyte nuclear factor 1-Ī± (HNF1A) as a key regulator of fucosylation. We hypothesized that loss-of-function HNF1A mutations causal for maturity-onset diabetes of the young (MODY) would display altered fucosylation of N-linked glycans on plasma proteins and that glycan biomarkers could improve the efficiency of a diagnosis of HNF1A-MODY. In a pilot comparison of 33 subjects with HNF1A-MODY and 41 subjects with type 2 diabetes, 15 of 29 glycan measurements differed between the two groups. The DG9-glycan index, which is the ratio of fucosylated to nonfucosylated triantennary glycans, provided optimum discrimination in the pilot study and was examined further among additional subjects with HNF1A-MODY (n = 188), glucokinase (GCK)-MODY (n = 118), hepatocyte nuclear factor 4-Ī± (HNF4A)-MODY (n = 40), type 1 diabetes (n = 98), type 2 diabetes (n = 167), and nondiabetic controls (n = 98). The DG9-glycan index was markedly lower in HNF1A-MODY than in controls or other diabetes subtypes, offered good discrimination between HNF1A-MODY and both type 1 and type 2 diabetes (C statistic ā„ 0.90), and enabled us to detect three previously undetected HNF1A mutations in patients with diabetes. In conclusion, glycan profiles are altered substantially in HNF1A-MODY, and the DG9-glycan index has potential clinical value as a diagnostic biomarker of HNF1A dysfunction
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Demonstration Report: Handheld UXO Discriminator, SERDP No. MR-1667
In 2003, the Defense Science Board observed: 'The problem is that instruments that can detect the buried UXOs also detect numerous scrap metal objects and other artifacts, which leads to an enormous amount of expensive digging. Typically 100 holes may be dug before a real UXO is unearthed! The Task Force assessment is that much of this wasteful digging can be eliminated by the use of more advanced technology instruments that exploit modern digital processing and advanced multi-mode sensors to achieve an improved level of discrimination of scrap from UXOs.' In keeping with these remarks and with prior funding (UX-1225, MM-0437, and MM-0838), the LBNL group has successfully designed and built the cart-mounted Berkeley UXO Discriminator (BUD) and demonstrated its performance at various test sites (cf. Gasperikova et al., 2007, 2008, and 2009). Because hand-held systems have the advantage of being lightweight, compact, portable, and deployable under most site conditions, they are particularly useful in areas of dense vegetation or challenging terrain. In heavily wooded areas or areas with steep or uneven terrain, hand-held sensors may be the only suitable device for UXO detection and discrimination because it can be carried through spaces that the operator could walk through or at least approach. Furthermore, it is desirable to find and characterize a metallic object without the need to accurately locate the sensors at multiple positions around the target. The ideal system would thus locate and characterize the target from a single position of the sensor and indicate to the operator where to flag the target for subsequent study. Based on these considerations, we designed and built a sensor package in a shape of a 14-in (0.35 m) cube. This hand-held prototype incorporates the key features of the cart-mounted system - (a) three orthogonal transmitters and ten pairs of receivers, and (b) difference or gradient measurements that significantly reduce the ambient and motion noise, and greatly enhance the sensitivity to the gradients of the target. The system characterizes the target from a single position. Results from a local test site were in a good agreement with theoretical performance calculations of such a device. This survey was designed to demonstrate performance of the system under realistic survey conditions at the Aberdeen Proving Ground (APG) in Aberdeen, MD. The survey was preformed in an area with known items ('Calibration Grid'), and in a seeded blind test area (the 'Blind Test Grid'). The ground truth for the surveys conducted on the Blind Test Grid was withheld from the testers. Only the graded test scores based on target detection and target classification were provided. For more information, see http://aec.army.mil/usaec/technology/uxo01c03.html
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ESTCP Munitions Response Live Site Demonstration at Former Camp Beale, CA: LBNL Hand-Held UXO Discriminator MR-201166 Demonstration Report
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