YoeB toxin is activated during thermal stress.

Type II toxin-antitoxin (TA) modules are thought to mediate stress-responses by temporarily suppressing protein synthesis while cells redirect transcription to adapt to environmental change. Here, we show that YoeB, a ribosome-dependent mRNase toxin, is activated in Escherichia coli cells grown at elevated temperatures. YoeB activation is dependent on Lon protease, suggesting that thermal stress promotes increased degradation of the YefM antitoxin. Though YefM is efficiently degraded in response to Lon overproduction, we find that Lon antigen levels do not increase during heat shock, indicating that another mechanism accounts for temperature-induced YefM proteolysis. These observations suggest that YefM/YoeB functions in adaptation to temperature stress. However, this response is distinct from previously described models of TA function. First, YoeB mRNase activity is maintained over several hours of culture at 42°C, indicating that thermal activation is not transient. Moreover, heat-activated YoeB does not induce growth arrest nor does it suppress global protein synthesis. In fact, E. coli cells proliferate more rapidly at elevated temperatures and instantaneously accelerate their growth rate in response to acute heat shock. We propose that heat-activated YoeB may serve a quality control function, facilitating the recycling of stalled translation complexes through ribosome rescue pathways

Comparing stenotic blood flow in three- and two-dimensional arterial renderings using computational fluid dynamics and multiphase mean age theory.

Over one million invasive coronary angiography procedures are performed annually in patients who experience chest pain or are known to have coronary artery disease. The procedure is carried out to ascertain the degree of arterial blockage (stenosis) that hinders blood flow to the heart. A cardiologist performing the procedure determines the physiological degree of a stenosis by either visual estimation, which is routine practice, or by invasively measuring fractional flow reserve (FFR), which is the current gold standard that has been demonstrated to improve patient outcomes and temper the cost of healthcare. Nevertheless, FFR is performed in only 10–20% of patients because it is invasive, expensive, and requires more radiation exposure. New computational methods utilizing three-dimensional renderings processed from coronary angiograms can provide an accurate, highly sensitive, non-invasive method to assess stenotic significance without using FFR. While beneficial, this technique requires intensive computer processing power and calculation runtimes on the order of several hours. An approach to reduce computational time involves alike computing of two-dimensional arterial slices cut from the three-dimensional source renderings. The main objective was to determine if two-dimensional processing can also provide an accurate and highly sensitive method to assess stenotic significance at a fraction of the computational expense. Blood flow was analyzed in five patient cases below and five patient cases above the commonly accepted FFR threshold value for intervention of 0.80. Following the generation of two orthogonal slices from DICOM-derived three-dimensional renderings, pulsing blood flow was simulated with CFD, and multiphase mean age theory was applied to calculate the mean age of red blood cells as a diagnostic metric. Two-dimensional processing typically exhibited a correlation with FFR only in the geometries of vertically-oriented slices. This was ascribed to the possibility of uncaptured stenotic blood flow characteristics in the limited testing of only two angles of a full arterial segment.Mean ages for the three-dimensional cases were many orders of magnitude higher than those of the corresponding two-dimensional cases. This was attributed to red blood cell collisions and distal recirculatory eddies near a stenosisbeing less expressed in the simplicity of the two-dimensional slices when compared to the complexity of the three-dimensional source renderings. A mean age threshold for determining stent intervention was estimated for the two-dimensional cases since limited sample size disallowed rigorous statistical analysis. The data suggested an arbitrary value equal to ~2.5. Nine out of ten cases correlated with FFR, with just one false negative diagnosis. In published virtual FFR techniques, false diagnosis typically occurs in 10–13% of the cases. Computational runtime for two-dimensional cases was less than 2% of the runtime for corresponding three-dimensional cases. Preliminary results indicate two-dimensional processing may efficiently detect and assess stenoses non-invasively, provided that it holds up to rigorous statistical analysis following testing of at least 80–100 more cases, plus several additional slice angles

First language use by adequate formal schooling adult English language learners in a university English language institute

In their pursuit of a better way of life, immigrants who arrive in this country are faced with many challenges. One important challenge is their need to adapt to a new society that requires them to learn English as a second language (ESL). As adult learners enter ESL programs, they are confronted with a common sense belief that the exclusion of their native language (L1) and the exclusive use of the target language (TL) in their instruction is the best approach to learn English. Whether immigrant students are educated or not in their home countries, they face the double task of learning to speak and understand a new language, while also learning how to read and write in that same language. The purpose of this study, therefore, was to discover if and how a group of L1 educated adult learners used their first language as they learned and developed academic English in a university English language institute. An additional objective of the study was to examine the perceptions this sample group of adult students had towards their L1 as a learning and linguistic resource. Seven Spanish-speaking participants with a high school and/or college level L1 education were selected to take part in this qualitative study. Findings indicated that, in spite of some negative opinions expressed towards their L1, this group of L1 educated students used and relied on their L1 to develop academic English

Religious pro-sociality? Experimental evidence from a sample of 766 Spaniards

This study explores the relationship between several personal religion-related variables and social behaviour, using three paradigmatic economic games: the dictator (DG), ultimatum (UG), and trust (TG) games. A large carefully designed sample of the urban adult population in Granada (Spain) is employed (N = 766). From participants' decisions in these games we obtain measures of altruism, bargaining behaviour and sense of fairness/equality, trust, and positive reciprocity. Three dimensions of religiosity are examined: (i) religious denomination; (ii) intensity of religiosity, measured by active participation at church services; and (iii) conversion out into a different denomination than the one raised in. The major results are: (i) individuals with “no religion” made decisions closer to rational selfish behaviour in the DG and the UG compared to those who affiliate with a “standard” religious denomination; (ii) among Catholics, intensity of religiosity is the key variable that affects social behaviour insofar as religiously-active individuals are generally more pro-social than non-active ones; and (iii) the religion raised in seems to have no effect on pro-sociality, beyond the effect of the current measures of religiosity. Importantly, behaviour in the TG is not predicted by any of the religion-related variables we analyse. While the results partially support the notion of religious pro-sociality, on the other hand, they also highlight the importance of closely examining the multidimensional nature of both religiosity and pro-social behaviour

A Six-Sigma DMAIC Approach to Improve the Sales Process of a Technology Start-Up

Despite the adoption of Six-Sigma in different service sectors, its application in the Sales function of a Technology-based Start-up has not been explored. This paper deploys an action research-based study methodology and conducts a thorough analysis of a Technology Start-up company in Indonesia, using Six-Sigma principles and the Define-Measure-Analyse-Improve-Control (DMAIC) approach. Statistical validation of the causes of problems helped to formulate a strategy that may have otherwise not been possible. The results of the study and proposed solutions confirm the potential benefits of adopting Six-Sigma in the Sales function of technology start-ups to reduce, particularly, customer waiting time. The novelty of this research lies in the fact that it applies Six-Sigma in a transactional process such as sales, which earlier studies have not explored in depth. This paper can be employed as a reference for organisations to undertake and guide specific process improvement projects similar to the one presented

Ocular Fundus Photography as an Educational Tool

The proficiency of nonophthalmologists with direct ophthalmoscopy is poor, which has prompted a search for alternative technologies to examine the ocular fundus. Although ocular fundus photography has existed for decades, its use has been traditionally restricted to ophthalmology clinical care settings and textbooks. Recent research has shown a role for nonmydriatic fundus photography in nonophthalmic settings, encouraging more widespread adoption of fundus photography technology. Recent studies have also affirmed the role of fundus photography as an adjunct or alternative to direct ophthalmoscopy in undergraduate medical education. In this review, the authors examine the use of ocular fundus photography as an educational tool and suggest future applications for this important technology. Novel applications of fundus photography as an educational tool have the potential to resurrect the dying art of funduscopy

BCHS 2526 Health Disparities Research: Methods and Interventions

This course will examine the challenges and methods in the implementation of health disparities research and interventions. It is intended to both complement and expand the knowledge gained in other BCHS courses by focusing specifically on minority/underserved populations. The course will explore readings and foster discussions that will include: ethics and research in minority/underserved communities; issues, barriers and facilitators to engaging minority/underserved communities in health research; examining basic research questions in minority health; understanding the application of research findings to program development; how to integrate theory and research, and issues and challenges of program implementation. An overarching goal is to increase awareness and knowledge of research on minority health, as well as unique issues to consider when engaging in public health research and practice in these communities. The purpose of this class is to introduce basic issues, challenges, and opportunities that are encountered in health disparities research and interventions. Current research and resources from minority health and health disparities literature will provide the background for student assignments

Determinants of disaffiliation: an international study

Using a dataset of 15,000 subjects from 32 Western countries, the current study examines individuals who were raised in a certain religion and, at some stage of their lives, left it. Currently, they define their religious affiliation as ‘no religion’. A battery of explanatory variables (country-specific, personal attributes and marriage variables) was employed to test for determinants of this decision. It was found that the tendency of individuals to leave their religion—the most extreme symptom of secularization—is strongly correlated with their liberal beliefs and with parental and spousal religious characteristics. Moreover, country characteristics, as well as personal socio-demographic features seem to be much less relevant, except for the religious diversity of the country that has a positive effect on disaffiliation

Intergenerational transmission of ‘religious capital’. Evidence from Spain

This paper examines intergenerational transmission of ‘religious capital’ from parents to their offspring within an economic framework. The analytical tool is a ‘production function of religiosity’ where parental religious inputs serve as factors of production. The database used is based on a large-scale survey that was conducted in 1998 in Spain. In addition to information on the religious affiliation of the respondent and his parents, it has detailed data on two dimensions of the individual’s religious performance: church attendance and prayer. It also includes information on the mother’s and father’s church attendance when the respondent was a child, as well as the respondent’s participation in mass services at the age of 12. Socio-economic background data are also available. The core findings are: (i) parental religious inputs significantly affect individuals’ religiosity; (ii) interestingly, the route of intergenerational transmission is from mother to daughter and from father to son; and (iii) current mass participation of respondents is more affected by parental- than by own childhood mass attendance