Identification of novel bioactive peptides

Motivation: Targeting agents are the most commonly used strategies in therapeutics and diagnostic treatment.They can be classified in two main classes: chemicals and biologicals. Chemicals (farmaceutics and related) have been used for years, but nowadays biologicals experience an increasing demand because of their potentially higher specificity and affinity. Bioactive peptides from rational design can act as targeting agents that specifically interact with, and mostly inhibit, a biomolecule of interest (Seignauric et al., 2011).Methods: We have built a large library of peptides, and are screening for those that have biological activity, specially related to cell proliferation inhibition. The initial inconvenience is that small peptides cannot be expressed from single transcription unit as larger proteins, as they will be destroyed by cell proteases system. It is necessary to include the peptide library into a protein scaffold. We have designed a novel structure that includes the variable peptide sequence from the library between two mini-domains "EFLIVIKS" (one letter aminoacid code) able to form a noncovalent loop (Gururaja et al., 2010). In one of our constructs we have included a T7 promoter to allow conditional expression in bacteria. For its expression, we have designed two large nucleotide sequences (79 and 124 nucleotides, the large one includes the variable peptide sequence) that assemble by 20 complementary nucleotides in their 3' ends. By polymerization, both oligos strings are filled and form a two-chain structure, that will be inserted in a expression vector to obtain our peptide library. We transform bacteria (E. coli) and yeast (Schizosaccharomyces pombe) in order to identify those colonies excpressing the bioactive peptides that kill the cells or interrupt cell cycle

Secondary homotopy groups

Secondary homotopy groups supplement the structure of classical homotopy groups. They yield a track functor on the track category of pointed spaces compatible with fiber sequences, suspensions and loop spaces. They also yield algebraic models of homotopy types with homotopy groups concentrated in two consecutive dimensions.Comment: We added further commets and references to make the paper more easily readabl

The Second INTEGRAL AGN Catalogue

The INTEGRAL mission provides a large data set for studying the hard X-ray properties of AGN and allows testing of the unified scheme for AGN. We present analysis of INTEGRAL IBIS/ISGRI, JEM-X, and OMC data for 199 AGN supposedly detected by INTEGRAL above 20 keV. The data analysed here allow a significant spectral extraction on 148 objects and an optical variability study of 57 AGN. The slopes of the hard X-ray spectra of Seyfert 1 and Seyfert~2 galaxies are found to be consistent within the uncertainties, whereas higher cut-off energies and lower luminosities are measured for the more absorbed / type 2 AGN. The intermediate Seyfert 1.5 objects exhibit hard X-ray spectra consistent with those of Seyfert 1. When applying a Compton reflection model, the underlying continua appear the same in Seyfert 1 and 2 with photon index 2, and the reflection strength is about R = 1, when assuming different inclination angles. A significant correlation is found between the hard X-ray and optical luminosity and the mass of the central black hole in the sense that the more luminous objects appear to be more massive. There is also a general trend toward the absorbed sources and type 2 AGN having lower Eddington ratios. The black holemass appears to form a fundamental plane together with the optical and X-ray luminosity of the form Lv being proportional to Lx^0.6 M^0.2, similar to that found between radio luminosity Lr, Lx, and M. The unified model for Seyfert galaxies seems to hold, showing in hard X-rays that the central engine is the same in Seyfert 1 and 2, but seen under different inclination angles and absorption. (Abridged)Comment: 26 pages, 16 figures, accepted for publication in A&A. Corrections by language editor included in version

Rapid spectral transition of the black hole binary V404 Cyg

During the June 2015 outburst of the black hole binary V404 Cyg, rapid changes in the X-ray brightness and spectra were common. The INTEGRAL monitoring campaign detected spectacular Eddington-limited X-ray flares, but also rapid variations at much lower flux levels. On 2015 June 21 at 20 h 50 min, the 3-10 keV JEM-X data as well as simultaneous optical data started to display a gradual brightening from one of these low-flux states. This was followed 15 min later by an order-of-magnitude increase of flux in the 20-40 keV IBIS/ISGRI light curve in just 15 s. The best-fitting model for both the pre- and post-transition spectra required a Compton-thick partially covering absorber. The absorber parameters remained constant, but the spectral slope varied significantly during the event, with the photon index decreasing from $\Gamma \approx 3.7$ to $\Gamma \approx 2.3$. We propose that the rapid 20-40 keV flux increase was either caused by a spectral state transition that was hidden from our direct view, or that there was a sudden reduction in the amount of Compton down-scattering of the primary X-ray emission in the disk outflow.Comment: 6 pages, 2 figures, accepted to A&

Street lighting energy efficiency

Un estudio sobre la optimización de la eficiencia energética en el alumbrado público de la Municipalidad de S. M. de Tucumán fue efectuado basado en la aplicación de relojes astronómicos, balastos electrónicos dimerizables para lámparas de descarga de Sodio de alta presión de 150W/100W y luminarias de alta eficiencia. Durante 45 días, 273 puntos de luz conectados a 6 redes de alumbrado independientes fueron monitoreados, registrando el consumo, tiempos de funcionamiento, apagados nocturnos, encendidos diurnos y parámetros eléctricos de calidad. Los resultados indican que es posible una importante reducción en el consumo, entre un 11% y un 43% mejorando la calidad del servicio y reduciendo el contenido de armónicos que se introducen a la red de alumbrado a TDH A ≤ 6,5%. El trabajo describe la experiencia y el análisis de los resultados obtenidos.A study of street lighting energy efficiency was carried out in S. M. Tucumán City, based on the application of astronomical clocks, electronic ballasts (control gear) for high pressure sodium 150W/100W lamps and high efficiency luminaires. During 45 days, 273 luminaires connected to 6 independent electric networks were monitored recording: energy consumption, switching on/off times, night lamps outages, day on lamps and electrical quality parameters. The results indicate that it is possible a significant reduction in consumption, between 11% and 43% while improving service quality and reducing the harmonic content being introduced to the network lighting by TDH A ≤ 6.5%. The paper describes the experience and the analysis of the results.Fil: Tapia Garzón J.. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Luminotecnia, Luz y Visión; ArgentinaFil: Manzano, Eduardo Roberto. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Luminotecnia, Luz y Visión; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Investigación en Luz, Ambiente y Visión. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Instituto de Investigación en Luz, Ambiente y Visión; ArgentinaFil: Gao S.. Municipalidad de S. M. Tucuman - Dirección Alumbrado; ArgentinaFil: Rojas M.. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Luminotecnia, Luz y Visión; ArgentinaFil: De Nobrega M.. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Luminotecnia, Luz y Visión; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Investigación en Luz, Ambiente y Visión. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Instituto de Investigación en Luz, Ambiente y Visión; Argentin

Assessment of Peak Water Usage among Residential Consumers across Several Drinking Water Service Areas

[EN] Public drinking water service providers must comprehensively understand and effectively characterise user demands, especially during peak hours, which not only impact the maximum demand within the distribution network but also determine the dimensions of interior networks within buildings. Residential consumers show different consumption patterns based on socioeconomic factors, spatial location, climatic conditions and the consistency and quality of service delivered by public service providers. This study focused on assessing 1,317,584 users distributed across four distinct service areas in Bogota, Colombia. To achieve this, a stratified random sampling of 1233 residential subscribers was conducted and 320 reference digital Y290 Aquabus micro-meters were installed to characterise the four service areas. The installations were grouped into sets of 320 users until the entire sample of 1233 subscribers was encompassed. The results demonstrated that the rational method provided the most accurate fit for estimating the probable maximum flow rates compared to the values measured and, consequently, is the most suitable method for application within the region of interest. However, whereas the Hunter Unal method displayed a reasonable fit, it tended to underestimate the size of internal networks within buildings. The remaining methods, such as the British, square root, simultaneity, Hunter, NTC 1500 Hunter and Chilean methods, did not yield significant adjustments and tended to overestimate the probable maximum flow rates as well as the internal networks within buildings. The results indicate that, depending on the method used to calculate the probable maximum flow or design flow of the internal network, there can be a deviation factor when compared to the actual peak flow measured (real maximum flow). This deviation factor ranges from 0.79 (calculated less than measured) to 3.77 (calculated greater than measured). Additionally, a sizing case study was conducted, which involved applying all methods to a scenario involving a residential user. This study aimed to determine the variation expected in the estimation of the diameter of the supply pipe to the internal network when using the flow results from different methods. This analysis serves to conclude the research.Garzón-Orduña, AJ.; Coronado-Hernández, OE.; Ortiz, RO.; Arrieta-Pastrana, A.; Fuertes-Miquel, VS. (2024). Assessment of Peak Water Usage among Residential Consumers across Several Drinking Water Service Areas. Sustainability. 16(4):1-31. https://doi.org/10.3390/su1604160113116

Inhomogeneity correction of magnetic resonance images by minimization of intensity overlapping

Proceeding of: IEEE 2003 International Conference on Image Processing (ICIP), Barcelona, Spain, 14-17 Sept. 2003This work presents a new algorithm (NIC; Non uniform Intensity Correclion) for the correction of intensity inhomogeneities in magnetic resonance images. The algorithm has been validated by means of realistic phantom images and a set of 24 real images. Evaluation using previously proposed phantom images for inhomogeneity correction algorithms allowed us to obtain results fully comparable to the previous literature on the topic. This new algorithm was also compared, using a real image dataset, to other widely used methods which are freely available in the Internet (N3, SPM'99 and SPM2). Standard quality criteria have been used for determining the goodness of the different methods. The new algorithm showed better results removing the intensity inhomogeneities and did not produce degradation when used on images free from this artifact

A major star formation region in the receding tip of the stellar Galactic bar

We present an analysis of the optical spectroscopy of 58 stars in the Galactic plane at $l=27$\arcdeg, where a prominent excess in the flux distribution and star counts have been observed in several spectral regions, in particular in the Two Micron Galactic Survey (TMGS) catalog. The sources were selected from the TMGS, to have a $K$ magnitude brighter than +5 mag and be within 2 degrees of the Galactic plane. More than 60% of the spectra correspond to stars of luminosity class I, and a significant proportion of the remainder are very late giants which would also be fast evolving. This very high concentration of young sources points to the existence of a major star formation region in the Galactic plane, located just inside the assumed origin of the Scutum spiral arm. Such regions can form due to the concentrations of shocked gas where a galactic bar meets a spiral arm, as is observed at the ends of the bars of face-on external galaxies. Thus, the presence of a massive star formation region is very strong supporting evidence for the presence of a bar in our Galaxy.Comment: 13 pages (latex) + 4 figures (eps), accepted in ApJ Let

Long-term optical and X-ray variability of the Be/X-ray binary H 1145-619: Discovery of an ongoing retrograde density wave

Context. Multiwavelength monitoring of Be/X-ray binaries is crucial to understand the mechanisms producing their outbursts. H 1145-619 is one of these systems, which has recently displayed X-ray activity

Syngenta Enogen Feed Corn Containing an Alpha Amylase Expression Trait Improves Digestibility in Growing Calf Diets

Objective: To evaluate the digestibility parameters of growing cattle when fed Enogen Feed corn. Study Description: Seven cannulated Holstein steers were used to determine the effects on digestibility when fed Enogen Feed corn (Syngenta) as whole-corn or processed as dry-rolled at ad libitum intake. The Bottom Line: When Enogen Feed corn was fed in an ad libitum fashion to growing calves, dry matter and organic matter are digested to a greater extent relative to yellow corn