Demonstration of rapid-scan two-dimensional laser velocimetry in the Langley Vortex Research Facility for research in aerial applications

Tests were conducted to demonstrate a rapid scan two dimensional laser velocimeter (LV) measurement technique for aerial applications research. The LV system is capable of simultaneously measuring both vertical and axial flow velocity components in a near or far field vortex system. Velocity profiles were successfully measured in the wake vortex of a representative agricultural aircraft model, with the vortex system rapidly transporting in ground effect. Results indicate that the laser velocimetry technique can provide quantitative information of wake vortex characteristics in ground effect

A Scanning laser-velocimeter technique for measuring two-dimensional wake-vortex velocity distributions

A rapid scanning two dimensional laser velocimeter (LV) has been used to measure simultaneously the vortex vertical and axial velocity distributions in the Langley Vortex Research Facility. This system utilized a two dimensional Bragg cell for removing flow direction ambiguity by translating the optical frequency for each velocity component, which was separated by band-pass filters. A rotational scan mechanism provided an incremental rapid scan to compensate for the large displacement of the vortex with time. The data were processed with a digital counter and an on-line minicomputer. Vaporized kerosene (0.5 micron to 5 micron particle sizes) was used for flow visualization and LV scattering centers. The overall measured mean-velocity uncertainity is less than 2 percent. These measurements were obtained from ensemble averaging of individual realizations

Laser Doppler velocimetry application in the Langley 0.3-meter Transonic Cryogenic Tunnel

The problems and the potential use of a nonintrusive flow velocity measuring technique in the Langley 0.3 meter transonic cryogenic tunnel (TCT) were investigated. A laser velocimeter (LV) was used. It was concluded that free-stream velocity measurements can be successfully made in the Langley 0.3-m TCT using a low-power (15-mW) LV system. The measured and calculated mean velocities typically agreed within one percent. The overall normalized standard deviation was less than one percent. Tunnel vibration and temperature had no detrimental effects on the optical system. It is recommended that the LV work should be further investigated for future use in the Langley 0.3-m TCT

Laser velocimeter application to oscillatory liquid flows

A laser velocimeter technique was used to measure the mean velocity and the frequency characteristics of an oscillatory flow component generated with a rotating flapper in liquid flow system at Reynolds numbers approximating 93,000. The velocity information was processed in the frequency domain using a tracker whose output was used to determine the flow spectrum. This was accomplished with the use of an autocorrelator/Fourier transform analyzer and a spectrum averaging analyzer where induced flow oscillations up to 40 Hz were detected. Tests were conducted at a mean flow velocity of approximately 2 m/s. The experimental results show that the laser velocimeter can provide quantitative information such as liquid flow velocity and frequency spectrum with a possible application to cryogenic fluid flows

Laser velocimetry technique applied to the Langley 0.3 meter transonic cryogenic tunnel

A low power laser velocimeter operating in the forward scatter mode was used to measure free stream mean velocities in the Langley 0.3 Meter Transonic Cryogenic Tunnel. Velocity ranging from 51 to 235 m/s was measured. Measurements were obtained for a variety of nominal tunnel conditions: Mach numbers from 0.20 to 0.77, total temperatures from 100 to 250 K, and pressures from 101 to 152 kPa. Particles were not injected to augment the existing Mie scattering materials. Liquid nitrogen droplets were the existing liqht scattering material. Tunnel vibrations and thermal effects had no detrimental effects on the optical system

"I am your mother and your father!": In vitro derived gametes and the ethics of solo reproduction

In this paper, we will discuss the prospect of human reproduction achieved with gametes originating from only one person. According to statements by a minority of scientists working on the generation of gametes in vitro, it may become possible to create eggs from men’s non-reproductive cells and sperm from women’s. This would enable, at least in principle, the creation of an embryo from cells obtained from only one individual: ‘solo reproduction’. We will consider what might motivate people to reproduce in this way, and the implications that solo reproduction might have for ethics and policy. We suggest that such an innovation is unlikely to revolutionise reproduction and parenting. Indeed, in some respects it is less revolutionary than in vitro fertilisation as a whole. Furthermore, we show that solo reproduction with in vitro created gametes is not necessarily any more ethically problematic than gamete donation—and probably less so. Where appropriate, we draw parallels with the debate surrounding reproductive cloning. We note that solo reproduction may serve to perpetuate reductive geneticised accounts of reproduction, and that this may indeed be ethically questionable. However, in this it is not unique among other technologies of assisted reproduction, many of which focus on genetic transmission. It is for this reason that a ban on solo reproduction might be inconsistent with continuing to permit other kinds of reproduction that also bear the potential to strengthen attachment to a geneticised account of reproduction. Our claim is that there are at least as good reasons to pursue research towards enabling solo reproduction, and eventually to introduce solo reproduction as an option for fertility treatment, as there are to do so for other infertility related purposes

Quantitative Analysis of Immune Infiltrates in Primary Melanoma.

Novel methods to analyze the tumor microenvironment (TME) are urgently needed to stratify melanoma patients for adjuvant immunotherapy. Tumor-infiltrating lymphocyte (TIL) analysis, by conventional pathologic methods, is predictive but is insufficiently precise for clinical application. Quantitative multiplex immunofluorescence (qmIF) allows for evaluation of the TME using multiparameter phenotyping, tissue segmentation, and quantitative spatial analysis (qSA). Given that CD3+CD8+ cytotoxic lymphocytes (CTLs) promote antitumor immunity, whereas CD68+ macrophages impair immunity, we hypothesized that quantification and spatial analysis of macrophages and CTLs would correlate with clinical outcome. We applied qmIF to 104 primary stage II to III melanoma tumors and found that CTLs were closer in proximity to activated (CD68+HLA-DR+) macrophages than nonactivated (CD68+HLA-DR-) macrophages (P < 0.0001). CTLs were further in proximity from proliferating SOX10+ melanoma cells than nonproliferating ones (P < 0.0001). In 64 patients with known cause of death, we found that high CTL and low macrophage density in the stroma (P = 0.0038 and P = 0.0006, respectively) correlated with disease-specific survival (DSS), but the correlation was less significant for CTL and macrophage density in the tumor (P = 0.0147 and P = 0.0426, respectively). DSS correlation was strongest for stromal HLA-DR+ CTLs (P = 0.0005). CTL distance to HLA-DR- macrophages associated with poor DSS (P = 0.0016), whereas distance to Ki67- tumor cells associated inversely with DSS (P = 0.0006). A low CTL/macrophage ratio in the stroma conferred a hazard ratio (HR) of 3.719 for death from melanoma and correlated with shortened overall survival (OS) in the complete 104 patient cohort by Cox analysis (P = 0.009) and merits further development as a biomarker for clinical application

Lesbian and Heterosexual Two-Parent Families: Adolescent–Parent Relationship Quality and Adolescent Well-Being

This study compared 51 adolescents from intact two-mother planned lesbian families (all conceived through donor insemination) with 51 adolescents from intact mother-father families on their relationships with their parents (parental control, disclosure to parents, and adolescent-parent relationship quality), psychological adjustment (self-esteem, social anxiety, and conduct problems), and substance usage (consumption of tobacco, alcohol, and marijuana/hashish). The adolescents (average age 16 years) were matched on demographic characteristics (age, gender, educational level, country of birth, parental birth country) with a sample from a large school-based survey, and data were collected by means of adolescent self-reports. Analyses indicated that adolescents in both family types had positive relationships with their parents, which were favorably associated with psychological well-being. On the assessments of psychological adjustment and substance use, family type was significantly associated only with self-esteem and conduct problems: Adolescents with lesbian mothers had higher levels of self-esteem and lower levels of conduct problems than their counterparts in heterosexual-parent families. Overall, the findings indicate that adolescents from intact two-mother lesbian families are comparable to those in a matched comparison group with intact mother-father families. The few differences found on psychological well-being favored the adolescents in lesbian two-mother families