An update on the potential for male contraception : emerging options

The human population continues to grow and is estimated to rise to 10.1 billion by the end of the century. Therefore, there is still an unmet need for safe and highly effective contraceptive options for both men and women. Current options available to men include withdrawal, condoms, and vasectomy. Methods in development fall into two categories: hormonal and nonhormonal. This review will provide an overview of the testosterone combinations and immunocontraception of hormonal targets. Nonhormonal immunocontraception of sperm proteins will also be examined, together with the use of agents to disrupt other sperm-associated targets and pathways. The categories focused on include epididymal proteins, testicular kinases, epigenetic reader proteins, opioids, lonidamine derivatives, retinoic acid, microRNAs associated with spermatogenesis, and plant extracts. Considering these developments, the number of options available to men is likely to increase in the near future

Growth and yield responses to amending the sugarcane monoculture: interactions between break history and nitrogen fertiliser

Experiments were established in the Burdekin Irrigation Area in North Queensland, Australia, to measure whether yield improvements from breaking the sugarcane monoculture or fumigating the soil could be modified by the application of different rates of nitrogen (N) fertiliser. Experiments were conducted in consecutive crop cycles (phase 1, planted 1998; phase 2, planted 2001) using the variety Q117, with the interaction between N applications and rotation histories discussed for the two plant crops. Histories consisted of alternate crop, bare fallow or mixed grass-legume pastures for periods of 42-66 months, compared with continuous cane as plough-out replant without (PORP) or with (PORP-F) soil fumigation. The N strategies involved combinations of N rates (0-180kgN/ha) and application times (at planting, 90 days after planting (DAP) or split between these times) in phase 1 and N rates (0-300kgN/ha) in phase 2. Histories had differing effects on N available to the cane crop and hence on response to N fertiliser. Some combinations of history and N rate were N-limited and strong linear relationships between biomass production or cane yield and crop N content could be developed. Critical N contents for biomass production (R2≤0.93) and fresh-weight cane yield (R2≤0.88) were 1.42 and 0.57kgN/t, respectively. Application of N fertiliser was shown to have significant impacts on both tiller addition and the retention of tillers to produce harvestable stalks. However, the application of fertiliser N had limited (phase 1) or no (phase 2) capacity to provide the quantum of yield response in soil health benefits associated with breaking the sugarcane monoculture. Increasing N application rates above that required to optimise crop yield resulted in significant decreases in sugar content of cane and thus lower sugar yields. Yield increases solely from improved soil health (i.e. exclusive of N response) constituted advantages averaging 15% (phase 1) to 20% (phase 2) compared with PORP. These effects were manifest early in the establishment of primary shoots in the plant crops, although the longevity of these benefits was limited. Replanting cane after a 3-year crop cycle (plant, 1st and 2nd ratoon) on land that had been under pasture, crop, bare fallow or PORP-F histories (phase 2, cycle 2) showed carryover effects of histories on N availability and fertiliser N responsiveness, but limited yield impacts attributable to residual soil health benefits. These results reinforce the importance of crop rotation during breaks between sugarcane cycles to maintain soil health and improve crop productivity

Improving screening recall services for women with false-positive mammograms: a comparison of qualitative evidence with UK guidelines.

Published onlineResearch Support, Non-U.S. Gov'tOBJECTIVES: To gain an understanding of the views of women with false-positive screening mammograms of screening recall services, their ideas for service improvements and how these compare with current UK guidelines. METHODS: Inductive qualitative content analysis of semistructured interviews of 21 women who had false-positive screening mammograms. These were then compared with UK National Health Service (NHS) guidelines. RESULTS: Participants' concerns about mammography screening recall services focused on issues of communication and choice. Many of the issues raised indicated that the 1998 NHS Breast Screening Programme guidelines on improving the quality of written information sent to women who are recalled, had not been fully implemented. This included being told a clear reason for recall, who may attend with them, the length of appointment, who they will see and what tests will be carried out. Additionally women voiced a need for: reassurance that a swift appointment did not imply they had cancer; choice about invasive assessment or watchful waiting; the offer of a follow-up mammogram for those uncertain about the validity of their all-clear and an extension of the role of the clinical nurse specialist, outlined in the 2012 NHS Breast Screening Programme (NHSBSP) guidelines, to include availability at the clinic after the all-clear for women with false-positive mammograms. CONCLUSIONS: It is time the NHSBSP 1998 recall information guidelines were fully implemented. Additionally, the further suggestions from this research, including extending the role of the clinical nurses from the 2012 NHSBSP guidelines, should be considered. These actions have the potential to reduce the anxiety of being recalled.This paper presents independent research funded by the National Institute for Health Research (NIHR). This was partially through their Collaboration for Leadership in Applied Health Research and Care (CLAHRC) for the South West Peninsula and partially through the HTA Programme

The D3/2 Neutron Scattering Resonance in the Shell Model of O16

Model Hamiltonian for neutron scattering resonance in shell model of molecular oxyge

Images in cardiovascular medicine : multiphoton microscopy for three-dimensional imaging of lymphocyte recruitment into apolipoprotein-E-deficient mouse carotid artery

Two recent elegant studies have shown that in apolipoprotein-E– deficient mice, the lamina adventitia is a major site of arterial wall inflammation associated with lymphocyte infiltration into atherosclerotic arteries and with formation of adventitial lymphoid-like tissues.1,2 These results suggest that lymphocyte responses in the lamina adventitia may play a crucial role in atherosclerosis development.1,

The usability of climate information in sub-national planning in India, Kenya and Uganda: the role of social learning and intermediary organisations

Research on using climate information has often focused on the interaction between users and producers and the technical fit of information for real decision-making. However, due to resource and capacity constraints within both user and producer communities, this approach will not always be feasible or indeed necessary depending on the decisions at hand. These contexts have been relatively under-explored by scholars, and this paper provides an original empirical contribution using three case studies of sub-national governments in India, Kenya and Uganda. In the paper, we analyse how social learning supports changing the usability of climate information and the role of intermediary organisations in these processes. Firstly, the paper shows that intermediaries often choose to build the commitment to project aims rather than using climate information as an entry point to working on climate change, and this allows them to instigate challenging learning processes. Secondly, there are barriers to iterative processes and critical reflection with government stakeholders but these processes can gain traction when built into institutional practices such as formal M&E processes. Lastly, social learning can broaden the framing of climate change from a single sector issue to a multi-sectoral one. We conclude by arguing that bringing together scholarship on social learning with that on the usability of climate information can deepen understanding of the dynamic context in which the information becomes usable. The evidence from the case studies shows that learning processes can alter this context across scales

Preventing unintentional injuries to children under 15 years in the outdoors: a systematic review of the effectiveness of educational programs.

This is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record.Unintentional injuries to children in the outdoors have a significant impact on child mortality, development and healthcare costs. This paper presents the findings of a systematic review about the effectiveness of programs that provided information, advice or education about the prevention of unintentional injuries to children under 15 years during outdoor play and leisure.Centre for Public Health Excellence, National Institute for Health and Clinical Excellence (UK

Antigen depot is not required for alum adjuvanticity

Alum adjuvants have been in continuous clinical use for more than 80 yr. While the prevailing theory has been that depot formation and the associated slow release of antigen and/or inflammation are responsible for alum enhancement of antigen presentation and subsequent T- and B-cell responses, this has never been formally proven. To examine antigen persistence, we used the chimeric fluorescent protein EαGFP, which allows assessment of antigen presentation in situ, using the Y-Ae antibody. We demonstrate that alum and/or CpG adjuvants induced similar uptake of antigen, and in all cases, GFP signal did not persist beyond 24 h in draining lymph node antigen-presenting cells. Antigen presentation was first detectable on B cells within 6–12 h of antigen administration, followed by conventional dendritic cells (DCs) at 12–24 h, then finally plasmacytoid DCs at 48 h or later. Again, alum and/or CpG adjuvants did not have an effect on the magnitude or sequence of this response; furthermore, they induced similar antigen-specific T-cell activation in vivo. Notably, removal of the injection site and associated alum depot, as early as 2 h after administration, had no appreciable effect on antigen-specific T- and B-cell responses. This study clearly rules out a role for depot formation in alum adjuvant activity