COW URINE: AN ELIXIR

Cow urine therapy and all traditional system from indian system of medicine have a strong scientific base. Cow urine has been described in ‘SushritaSamhita' and ‘AshtangaSangraha' to be the most effective substance/secretion of animal origin with innumerable therapeutic values. Ancient books on ayurveda states that consumption of cow urine increase resistance to diseases by upto 104percent. In India, drinking of cow urine has been practiced for thousands of years. Panchagavya is a term used in Ayurveda to describe five important substances obtained from cow namely Urine, Dung, Milk, Ghee and Curd. Many people use Panchgavya in some rituals (Puja) and for medicinal purposes. It also used in Yajur Veda for Ark as a medicine. By reviewing the literature and research articleswe reached at the conclusion that it has several different activities like antioxidant, antidiabetic, wound healing property,      immunomodulator, also act as bioenhancer to increase the efficacy of antibiotics, nutrients, anticancer drugs liketaxol. Keywords: DPHH,  fetal calf serum (FCS), NBT methodÂ

Macrophage Polarization Is Decisive for Chronic Bacterial Infection-Induced Carcinogenesis

Macrophages are the special cells of the immune system and play both immunological and physiological role. One of the peculiar characteristics of macrophages is that they are double-edged and highly plastic component of immune system. Due to this characteristic, they are responsible for both progressions as well control of a variety of inflammatory, infectious and metabolic diseases and cancer. These are found in the body in three major phenotypes, which are known as M0 (also known as naïve); M1 (classically activated macrophages); and/or M2 (alternatively activated macrophages) at normal physiological conditions. We have been exploring macrophages in context of bacterial infection and previously demonstrated that M2 polarization of M1 effector alveolar macrophages during chronic/persistent Chlamydia pneumonia, Mycobacterium tuberculosis and Helicobacter pylori pathogens are decisive for the infection induced cancer development in host. Since chronic infection with these pathogens has been associated with adenocarcinoma, therefore, we feel that disruption of macrophage plasticity plays crucial role in the host for the development of cancer. On the basis of this, we propose that in such pathological conditions, management of M1/M2 imbalance is paramount for minimizing the risk of developing cancer by chronic and persistent infection

Adrenal ganglioneuroma; a rare neurogenic tumor in a 25-year-old female

Ganglioneuromas (GNs) are rare benign tumors originating from the neural crest tissue. They are characteristically located in the posterior mediastinum and retroperitoneum; and are rarely found in the adrenal gland. GNs are usually hormonally inactive, and most of the cases are detected incidentally. We report a case of 25-year-old female who presented with pain abdomen in the right upper quadrant. Imaging studies showed a large well defined hypodense lesion with calcification measuring 14.5×11.5×11cm in the region of right adrenal gland and a possibility of adrenocortical carcinoma was suggested. The patient underwent right adrenalectomy and histopathological examination revealed ganglioneuroma. This report emphasizes that GN can be misdiagnosed preoperatively as the presenting symptoms are nonspecific and imaging characteristics are variable. Histopathological examination is the mainstay of diagnosis

A histopathological analysis of prevalence of various heart diseases: an autopsy study

Background: Objective was to study the histopathological spectrum of heart diseases in autopsy specimens, that play a major role as cause of death.Methods: During the period from October 2015 to October 2017. Total 170 medicolegal autopsies were received during this period. Out of 170, specimens of heart were 150. Nine specimens were autolyzed. So, 141 specimen of heart were included in the study. Gross and microscopic findings on H and E stained sections were studied.Results: out of 141 cases, 78 cases showed atherosclerosis, 20 cases showed features of myocardial infarction, myocardial hypertrophy was found in 10 cases, 5 cases revealed myocarditis, pericarditis in 4 cases and one case each of infective endocarditis and aortic stenosis. In 22 cases, there was no identifiable cause of death even after complete gross and microscopic autopsy was performed.Conclusions: Myocardial infarction due to atherosclerosis is probably the commonest finding in death cases subjected to medicolegal autopsies

Bibliometrics of Indian veterinary science research output during 2001-2020

411-421The paper is an analysis of 7,056 papers published by Indian scientists during 2001-2020 in the discipline of veterinary sciences. The study indicates that there is a steep decline in the Indian research output in veterinary sciences after the two years block of 2009-2010. Most of the prolific institutions except Sri Venkateswara University, Tirupati were funded by Indian Council of Agricultural Research (ICAR) published about 71% of the total output. Of these, Indian Veterinary Research Institute (IVRI), Izatnagar produced the highest number of papers and Central Institute of Fisheries and Aquaculture (CIFA), Bhubaneswar had the highest value of CPP. Highest number of prolific authors was also from Indian Veterinary Research Institute (IVRI). About three-fourth of the papers were published in low impact factor journals. Of the total published papers, about one-third remained uncited. All the highly cited papers were written either in domestic or international collaboration. About 45% papers were published in journals of Indian origin and the remaining papers in journals originating from other countries. Among countries from abroad, highest number of papers was published in journals originating from USA and UK

Bibliometrics of Indian veterinary science research output during 2001-2020

The paper is an analysis of 7,056 papers published by Indian scientists during 2001-2020 in the discipline of veterinary sciences. The study indicates that there is a steep decline in the Indian research output in veterinary sciences after the two years block of 2009-2010. Most of the prolific institutions except Sri Venkateswara University, Tirupati were funded by Indian Council of Agricultural Research (ICAR) published about 71% of the total output. Of these, Indian Veterinary Research Institute (IVRI), Izatnagar produced the highest number of papers and Central Institute of Fisheries and Aquaculture (CIFA), Bhubaneswar had the highest value of CPP. Highest number of prolific authors was also from Indian Veterinary Research Institute (IVRI). About three-fourth of the papers were published in low impact factor journals. Of the total published papers, about one-third remained uncited. All the highly cited papers were written either in domestic or international collaboration. About 45% papers were published in journals of Indian origin and the remaining papers in journals originating from other countries. Among countries from abroad, highest number of papers was published in journals originating from USA and UK

Visual evoked potentials in children with type 1 diabetes mellitus

Background: Insulin-dependent (type 1) diabetes mellitus is associated with different degrees of neuropathies affecting peripheral as well as central neural pathways. The subclinical neurological involvement in diabetic children can be assessed by changes appearing in the electrophysiological parameters like Visual Evoked Potentials (VEPs). The objective of the study was to assess the effect of type I diabetes melitus on the visual evoked potentials in children.Methods: This cross-sectional case-control study was done on 30 children with type 1 diabetes mellitus of minimum two years duration as cases and 30 age and gender matched euglycemic children with normal HbA1c taken as controls. Visual Evoked Potentials (VEPs) were done on both the groups of children and the latencies (ms) of waves -N75, P100, N145 and amplitude (μV) of wave P100 were recorded. The data was compiled in the pre-designed proforma and statistically analysed using student t-test.Results: The increase in the mean latencies of waveforms of VEPs N75, P100 and amplitude P100 were found to be highly significant statistically (p<0.001) in both the eyes among the children with type 1 diabetes mellitus. The mean latency of waveform N145 was found to be statistically insignificant in the two groups (p>0.05).Conclusions: The type 1 diabetes does affect the visual pathways in children. Visual Evoked Potentials are helpful in the detection of early changes in the conduction across the neural pathways in the sub-clinical diseases

Serum Proteomic Signature of Human Chagasic Patients for the Identification of Novel Potential Protein Biomarkers of Disease

Chagas disease is initiated upon infection by Trypanosoma cruzi. Among the health consequences is a decline in heart function, and the pathophysiological mechanisms underlying this manifestation are not well understood. To explore the possible mechanisms, we employed IgY LC10 affinity chromatography in conjunction with ProteomeLab PF2D and two-dimensional gel electrophoresis (2D-GE) to resolve the proteome signature of high-abundance and low-abundance serum proteins in chagasic patients. MALDI-TOF MS/MS analysis yielded 80 and 14 differentially expressed proteins associated with cardiomyopathy of chagasic and other etiologies, respectively. The extent of oxidative stress-induced carbonyl modifications of the differentially expressed proteins (n=26) was increased and coupled with a depression of antioxidant proteins. Functional annotation of the top networks developed by Ingenuity Pathway Analysis of proteome database identified dysregulation of inflammation/acute phase response signaling and lipid metabolism relevant to production of prostaglandins and arachidonic acid in chagasic patients. Overlay of the major networks identified prothrombin and plasminogen at a nodal position with connectivity to proteome signature indicative of heart disease (i.e. thrombosis, angiogenesis, vasodilatation of blood vessels or the aorta, increased permeability of blood vessel and endothelial tubes), and inflammatory responses (e.g., platelet aggregation, complement activation, phagocytes activation and migration). The detection of cardiac proteins (myosin light chain 2, myosin heavy chain 11) and increased levels of vinculin and plasminogen provided a comprehensive set of biomarkers of cardiac muscle injury and development of clinical Chagas disease in human patients. These results provide an impetus for biomarker validation in large cohorts of clinically characterized chagasic patientsFil: Wen, Jian Jun. University of Texas Medical Branch; Estados UnidosFil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Nuñez, Sonia. Provincia de Salta. Hospital San Bernardo; ArgentinaFil: Gupta, Shivali. University of Texas Medical Branch; Estados UnidosFil: Nuñez Burgos, Federico. Provincia de Salta. Hospital San Bernardo; ArgentinaFil: Garg, Nisha Jain. University of Texas Medical Branch; Estados Unido

Identification and Characterization of Trimethylamine-N-oxide Uptake and Efflux Transporters

Trimethylamine-N-oxide (TMAO) is a recently identified predictor of cardiovascular and chronic kidney disease. TMAO is primarily generated through gut-microbiome mediated conversion of dietary choline and carnitine to TMA, which is converted to TMAO by hepatic flavin monooxygenase 3 (FMO3) and subsequently undergoes renal elimination. We investigated the role of uptake and efflux drug transporters in TMAO disposition in vitro and in vivo. After screening a large array of uptake transporters, we show organic cation transporter 2 (OCT2) is the key transporter for TMAO cellular uptake. In Oct1/2 knockout mice, we observed increased plasma TMAO levels with reduced renal retention, suggesting the importance of Oct2 in facilitating the uptake of TMAO into renal tubular cells in vivo. Multiple transporters of the ATP-binding cassette (ABC) family, including ABCG2 (BCRP) and ABCB1 (MDR1), were capable of TMAO efflux. In human subjects, clinical, dietary, and pharmacogenetic covariates were evaluated for contribution to TMAO levels in a cohort of dyslipidemic patients (n = 405). Interestingly, genetic variation in ABCG2, but not other transporters, appeared to play a role in modulating TMAO exposure

Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease

Chagas disease is the most common cause of congestive heart failure related deaths among young adults in the endemic areas of South and Central America and Mexico. Diagnosis and treatment of T. cruzi infection has remained difficult and challenging after 100 years of its identification. In >95% of human cases, T. cruzi infection remains undiagnosed until several years later when chronic evolution of progressive disease results in clinical symptoms associated with cardiac damage. Diagnosis generally depends on the measurement of T. cruzi'specific antibodies that can result in false positives. A conclusive diagnosis of T. cruzi infection thus often requires multiple serological tests, in combination with epidemiological data and clinical symptoms. In this study, we investigated the antibody response to TcG1, TcG2, and TcG4 in clinically characterized chagasic patients. These antigens were identified as vaccine candidates and shown to elicit protective immunity to T. cruzi and Chagas disease in experimental animals. Our data show the serology test developed using the TcGmix (multiplex ELISA) is a significantly better alternative to epimastigote extracts currently used in T. cruzi serodiagnosis or the trypomastigote lysate used in this study for comparison purposes.Fil: Gupta, Shivali. University Of Texas Medical Branch. Department Of Pathology; Estados UnidosFil: Wan, Xianxu. University Of Texas Medical Branch. Department Of Pathology; Estados UnidosFil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; ArgentinaFil: Martinez Sellers, Valena C.. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Silva, Trevor S.. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Assiah, Dadjah. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Dhiman, Monisha. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Nuñez, Sonia. Provincia de Salta. Hospital Público de Gestion Descentralizada San Bernardo; ArgentinaFil: Petersen, John R.. University Of Texas Medical Branch. Department of Pathology; Estados UnidosFil: Vazquez Chagoyán, Juan C.. Universidad Autónoma de Estado de México ; MéxicoFil: Estrada Franco, Jose G.. Universidad Autónoma de Estado de México; MéxicoFil: Garg, Nisha Jain. University Of Texas Medical Branch. Department of Pathology; Estados Unido