Modelo de selección de personal basado en la gestión del conocimiento en una pyme manufacturera colombiana

Este estudio tuvo como objetivo construir un modelo de selección de personal basado en la gestión del conocimiento que pueda ser aplicado en una PYME manufacturera haciendo que esta sea más eficiente en la elección de los candidatos, logrando retener y potenciar al talento humano para así encaminar a la empresa y que esta pueda posicionarse como una organización competitiva, en la que exista el desarrollo y fortalecimiento del conocimiento de cada empleado, siendo esto, el aspecto que hace valiosa y única a una empresa porque es lo que le pertenece a la misma. Para el desarrollo de esta investigación se revisaron conceptos y temáticas relacionados con la gestión de conocimiento, los activos intangibles, la selección de personal y las competencias, desde el contexto global actual. Para llevar a cabo esta investigación, se realizaron observaciones, entrevistas semi-estructuradas y un grupo focal, las cuales permitieron conocer las necesidades y dinámicas relacionales que evidenciaron la cultura organizacional y la transferencia de conocimiento. Teniendo en cuenta lo anterior, se crearon unas competencias organizacionales y se hallaron los cargos críticos de la PYME, lo cual permitió la construcción del modelo de selección de personal basado en la gestión del conocimiento.The study´s objective was build a personal selection model on knowledge management that can be applied in a PYME manufacturing company, making it more efficient on the selection of the people, achieving retain and enhance the human talent in order to steer the company to position itself as a competitive organization, in which exists the development and strengthen knowledge of each employees, that is the aspect that makes a valuable and unique company because it is what belongs to it. For the development of this research were reviewed concepts and issues related to knowledge management, intangible assets, personnel selection and competencies, from the current global context. To carry out this investigation, observations were made, semi-structured interviews and focus group, which allowed determining the needs and relational dynamics that demonstrated the organizational culture and knowledgetransfer. From this organizational competencies were created and found the critical positions of the PYME, which allowed the construction of the recruitment model based on knowledge management.Psicólogo (a)Pregrad

Mouse and human tau expression in different brain areas

Background: An increase in tau protein is believed to be necessary for tau aggregation. However, whether this is due to increased expression of the endogenous tau promoter or protein accumulation due to proteostasis failure remains uncertain. Objective: To analyze the expression of GFP protein under endogenous tau promoter across different ages and within different brain areas. Methods: We have measured direct expression of Mapt gene promotor by western blot and immunofluorescence, by means of a commercial tau knock-out mice generated by integrating GFP-encoding cDNA into exon 1 of the Mapt gene. Besides, we have analyzed the MAPT gene expression in human samples. Results: Mapt expression is similar in the cortex, hippocampus, and cerebellum in mice and in human samples although some differences exist between dentate gyrus and CA1 hippocampal areas in mice. Besides, we have analyzed the murine Mapt gene expression during aging (at 2, 6, 12, and 18 moths) and no differences in endogenous tau promoter expression were observed. Conclusion: Our results suggest that Mapt promoter activity is similar in the brain areas studied and, therefore, tau accumulation due to aging is likely due to proteostasis failure rather than occurring at the transcriptional levelWork in the laboratory of JA is funded by grants from the Spanish Ministry of Economy and Competitiveness (PGC-2018-09177-B-100). Work in the laboratory of FH is funded by grants from the Spanish Ministry of Economy and Competitiveness (Ministerio de Economía, Industria y Competitividad, Gobierno de España, PID2020-113204GB-I00) and was co-financed from the Comunidad de Madrid through Structural Funds of the European Union [S2017/BMD-3700 (NEUROMETAB-CM)

Dysregulation of Connexin expression plays a pivotal role in psoriasis

Background: Psoriasis, a chronic inflammatory disease affecting 2–3% of the population, is characterised by epidermal hyperplasia, a sustained pro-inflammatory immune response and is primarily a T-cell driven disease. Previous work determined that Connexin26 is upregulated in psoriatic tissue. This study extends these findings. Methods: Biopsies spanning psoriatic plaque (PP) and non-involved tissue (PN) were compared to normal controls (NN). RNA was isolated and subject to real-time PCR to determine gene expression profiles, including GJB2/CX26, GJB6/CX30 and GJA1/CX43. Protein expression was assessed by immunohistochemistry. Keratinocytes and fibroblasts were isolated and used in 3D organotypic models. The pro-inflammatory status of fibroblasts and 3D cultures was assessed via ELISA and RnD cytokine arrays in the presence or absence of the connexin channel blocker Gap27. Results: Connexin26 expression is dramatically enhanced at both transcriptional and translational level in PP and PN tissue compared to NN (>100x). In contrast, CX43 gene expression is not affected, but the protein is post-translationally modified and accumulates in psoriatic tissue. Fibroblasts isolated from psoriatic patients had a higher inflammatory index than normal fibroblasts and drove normal keratinocytes to adopt a “psoriatic phenotype” in a 3D-organotypic model. Exposure of normal fibroblasts to the pro-inflammatory mediator peptidoglycan, isolated from Staphylococcus aureus enhanced cytokine release, an event protected by Gap27. Conclusion: dysregulation of the connexin26:43 expression profile in psoriatic tissue contributes to an imbalance of cellular events. Inhibition of connexin signalling reduces pro-inflammatory events and may hold therapeutic benefit

Archivos, posicionada en la senda del impacto

Con este bagaje, comenzamos 2023, un año en el que nuestra revista cumple un cuarto de siglo llevando a cabo la misión para la que fue concebida, misión que los distintos comités editoriales que hemos participado consideramos un acierto: contribuir a la transferencia de conocimiento útil para avanzar en la mejora de la salud de las personas trabajadora

Novel compound shows in vivo anthelmintic activity in gerbils and sheep infected by Haemonchus contortus

[EN] The control of gastrointestinal nematodes in livestock is becoming increasingly difficult due to the limited number of available drugs and the rapid development of anthelmintic resistance. Therefore, it is imperative to develop new anthelmintics that are effective against nematodes. Under this context, we tested the potential toxicity of three compounds in mice and their potential anthelmintic efficacy in Mongolian gerbils infected with Haemonchus contortus. The compounds were selected from previous in vitro experiments: two diamine (AAD-1 and AAD-2) and one benzimidazole (2aBZ) derivatives. 2aBZ was also selected to test its efficacy in sheep. In Mongolian gerbils, the benzimidazole reduced the percentage of pre-adults present in the stomach of gerbils by 95% at a dose of 200 mg/kg. In sheep, there was a 99% reduction in the number of eggs shed in faeces after 7 days at a dose of 120 mg/kg and a 95% reduction in the number of worm adults present in the abomasum. In conclusion, 2aBZ could be considered a promising candidate for the treatment of helminth infections in small ruminantsSIFinancial support came from MINECO: RETOS (AGL2016-79813-C2-1R/2R) and MICINN/AEI (PID2020- 119035RB-100). EVG was funded by FPU17/00627, FPU17/05346; VCGA, MAB, MCP and LGP are recipients of Junta de Castilla y León (JCyL) (LE082-18, LE051-18, LE135-19, LE096-20, respectively) and MMV by the Spanish “Ramon y Cajal” Programme (Ministerio de Economía y competitividad; MMV, RYC-2015-18368

Percepción de universitarios y bachilleres respecto del aprovechamiento tras 17 meses de confinamiento por covid-19

The objective of the work was to know the feelings of university students and high school students after 17 months confinement by COVID-19, focus to experience online classes. It is a quantitative study, an online survey of 648 students was used as a data collection instrument and descriptive statistics were used for the analysis. The results show the perceived advantages and disadvantages, as well as their level of academic achievement, 54 percent of students mention that it decreased. The analysis showed interesting data about physical distractors, 323 students answer the cell phone, 324 lose interest due to the poor quality of the network. 464 consider saving in transportation and food expenses an advantage, 462 saved in travel time and 392 say they have discovered new skills in the use of information technology. 77 percent mention that the workload increased; surprisingly, 78 percent have not stopped attending virtual classes. After analyzing the results, the need for the teacher to implement effective strategies to capture, maintain attention and interact with students in the present moment of the virtual class is perceived.El objetivo del trabajo fue conocer el sentir de los alumnos universitarios y bachilleres luego de 17 meses de confinamiento por COVID-19, con respecto de la experiencia y aprovechamiento de las clases en línea. Es un estudio cuantitativo, se empleó como instrumento de recolección de datos una encuesta en línea a 648 discentes y se utilizó estadística descriptiva para el análisis de la información.  Los resultados muestran las ventajas y desventajas percibidas, así como su nivel de aprovechamiento académico dónde, el 54 por ciento manifiesta que disminuyó. Con respecto de los distractores 323 alumnos atienden el celular, 324 pierden interés por la mala calidad de la red. 464 consideran una ventaja el ahorro en el gasto de transporte y alimento, 462 ahorraron en tiempo de traslado y 392 dicen haber descubierto nuevas habilidades en el uso de las TIC.  El 77 por ciento manifiesta que aumentó la carga de trabajo; sorprendentemente, el 78 por ciento no ha dejado de asistir a las clases virtuales. Luego del análisis de los resultados se percibe la necesidad de que el docente implemente estrategias efectivas para captar, mantener la atención e interactuar con los estudiantes en el momento presente de la clase virtual

El Sinemuriense superior: cronozonas Obtusum y Oxynotum en Asturias,España. Ammonoideos y correlación con otras cuencas del oeste de Europa

Se estudia la cronoestratigrafía, biosedimentación y, en particular, la sucesión de los ammonoideos que, con un registro relativamente pobre, se encuentra en varias secciones del Sinemuriense superior (cronozonas Obtusum y Oxynotum) de la costa de Asturias, entre la Playa de Peñarrubia (Gijón) y la Playa de Vega (Ribadesella). Si se exceptúa el límite inferior de la cronozona Obtusum, todas las unidades cronoestratigráficas han podido ser caracterizadas con bastante precisión. En la cronozona Obtusum (subcronozonas Obtusum y Stellare) se suceden en el tiempo siete especies de Asteroceras (A. aff. confusum-A. confusum-A. obtusum-A. cf. margaritoides-A. stellare-A. blakei) y Aegasteroceras (A. sagittarium), que de forma puntual pueden estar asociadas a escasos Epophioceras, Xipheroceras, Promicroceras y Cymbites. La subcronozona Denotatus se caracteriza por la sucesión de tres especies de Eparietites (E. undaries-E. impendens-E. glaber) asociadas a raros Promicroceras. En la cronozona Oxynotum se registran Palaeoechioceras, Gagaticeras (G. cf. exortum-G. gagateum) y Oxynoticeras (O. simpsoni) en la subcronozona Simpsoni y Oxynoticeras (O. oxynotum) asociado a Cheltonia (C. accipitris) en la subcronozona Oxynotum. En la parte media y superior de esta subcronozona, se registran asimismo escasos Paroxynoticeras y escasos Bifericeras y Gleviceras. En el aspecto paleobiogeográfico, la sucesión obtenida es típica de la Provincia del NO Europeo con la inclusión de algunos elementos más propios de la Provincia Mediterránea en la parte media y superior de la cronozona Obtusum (Asteroceras cf. suevicum, E. glaber) y en la parte media de la cronozona Oxynotum (Paroxynoticeras salisburgense)

Immunomodulation of mesenchymal stem cells in discogenic pain

13 p.BACKGROUND CONTEXT: Back pain is a highly prevalent health problem in the world today and has a great economic impact on health-care budgets. Intervertebral disc (IVD) degeneration has been identified as a main cause of back pain. Inflammatory cytokines produced by macrophages or disc cells in an inflammatory environment play an important role in painful progressive degeneration of IVD. Mesenchymal stem cells (MSCs) have shown to have immunosuppressive and antiinflammatory properties. Mesenchymal stem cells express a variety of chemokines and cytokines receptors having tropism to inflammation sites. PURPOSE: This study aimed to develop an in vitro controlled and standardized model of inflammation and degeneration of IVD with rat cells and to evaluate the protective and immunomodulatory effect of conditioned medium (CM) from the culture of MSCs to improve the conditions presented in herniated disc and discogenic pain processes. STUDY DESIGN: This is an experimental study. METHODS: In this study, an in vitro model of inflammation and degeneration of IVD has been developed, as well as the effectiveness of CM from the culture of MSCs. RESULTS: Conditioned medium from MSCs downregulated the expression of various proinflammatory cytokines produced in the pathogenesis of discogenic pain such as interleukin (IL)-1β, IL-6, IL-17, and tumor necrosis factor (TNF). CONCLUSION: Mesenchymal stem cells represent a promising alternative strategy in the treatment of IVD degeneration inasmuch as there is currently no treatment which leads to a complete remission of long-term pain in the absence of drug

Stratigraphy and Biochronostratigraphy of the Lower Pliensbachian (Jurassic) from the Asturian basin (Northern Spain)

A stratigraphic study of the carbonate deposits from the interval between the uppermost Sinemurian and the base of the Upper Pliensbachian is presented herein. For this purpose, four expanded sections of the Asturian Basin, in Northern Spain, were sampled. The sections are located between Rodiles E section (Villaviciosa municipality) and Playa de Vega section (Ribadesella municipality). The ammonites recorded in over 100 successive levels of this sector enabled the substantial improvement of the biochronostratigraphic scale of reference; making it comparable with those defined for other basins of the Iberian Peninsula and other areas of the western Tethys. The lower boundary of the Pliensbachian is indicated by the first appearance of Gemmellaroceras aff. aenigmaticum (Gemmellaro) below the first appearance of the genus Apoderoceras. The upper boundary is defined by the first record of the Protogrammoceras. (Matteiceras) subgenus which in some levels is associated with Amaltheus stokesi (Sowerby). The boundaries between the zones and subzones were able to be established accurately in most cases. The ammonite assemblages are similar to those established for the NW European Province. Some intervals, however, include species typical of the Mediterranean Province. Among these, the Taylori Subchronozone (Gemmellaroceras) and the transition between the Jamesoni Subchronozone and the Valdani Subchronozone (Tropidoceras) can be highlighted. Additionally, taking into consideration the succession between of ammonite genera and species, 27 horizons were identified, 24 of which correspond to the Lower Pliensbachian, and the other 3 to the uppermost Sinemurian and the lowermost part of the Upper Pliensbachian. Moreover, the identification of 3 of the Lower Pliensbachian horizons were based upon the evolution of the Mediterranean Tropidoceras