Bacterias diazotroficas y solubilizadoras de fósforo aisladas de las especies forestles altoandinas colombianas Weinmannia tomentosa y Escallonia myrtilloides

Encenillo (Weinmannia tomentosa Lf) and pagoda (Escallonia myrtilloides Lf) are dominant native tree species that can be found in the forests of the complex Andean Guerrero (Cundinamarca, Colombia). Forty two phosphorus-solubilizing bacteria and 125 nitrogen-fixing strains were isolated from the rhizosphere of these trees, out of which the microbial strains presenting the best five results of dissolution of phosphate and acetylene reduction by plant species were selected. The diazotrophic strains selected were identified as close to Enterobacter gergoviae, Alcaligenes sp. (E13C and E12E strains), Enterobacter sp. (E14C.2 strain), Pseudomonas sp., Sphingomonas sp., Paenibacillus sp., Achromobacter sp., Flavobacterium adoratum, and Klebsiella pneumoniae, and as phosphate-solubilizingbacteria near to Pantoea agglomerans, Pantoea sp., Brevudimonas sp., Enterobacter sp. (E6C.1 strain), Burkholderia cepacia, Arthrobacter sp., Aeromicrobium sp., Corynebacterium sp., Pimelobacter sp. and Micrococcus sp. In order to perform preliminary in vivo tests with the chosen microorganisms, it was inoculated bacterial mixture to encenillo and pagoda seedlings and evaluated their longitudinal growth for three months under greenhouse conditions. The major averages were obtained with the inoculation of Enterobacter gergoviae and Pantoea sp. in encenillo seedlings (average increase of 51% and 57% greater than the controls without microbial inoculation and sterile molasses medium, respectively) and the mixture of all selected bacteria in pagoda (Stimulation of 16% and 32% compared to controls molasses and without bacterial inoculation, respectively). The results showed that all the combinations using microbial inocula significantly stimulated longitudinal growth (Duncan multiple range test P<0.05). The synergism shown by co-inoculation of diazotrophic bacteria and phosphate solubilizing bacteria indicate the potential of these microorganisms as plant growth promoters and biofertilizers.El encenillo (Weinmannia tomentosa L.f) y la pagoda (Escallonia myrtilloides L.f) son especies forestales nativas predominantes en bosques altoandinos del complejo Guerrero (Cundinamarca, Colombia). De la rizosfera de estos árboles se aislaron 42 bacterias solubilizadoras de fósforo y 125 fijadoras de nitrógeno, de las cuales se escogieron las cepas microbianas que presentaron los cinco mejores resultados de disolución de fosfato y reducción de acetileno por cada especie vegetal. Las cepas diazotróficas escogidas se identificaron como cercanas a Enterobacter gergoviae, Alcaligenes sp. (Cepas E12E y E13C), Enterobacter sp (cepa E14C.2), Pseudomonas sp., Sphingomonas sp., Paenibacillus sp., Achromobacter sp., Flavobacterium adoratum y Klebsiella pneumoniae; y como solubilizadoras de fósforo a Pantoea agglomerans, Pantoea sp., Brevudimonas sp., Enterobacter sp. (Cepa E6C.1), Burkholderia cepacia, Arthrobacter sp., Aeromicrobium sp., Corynebacterium sp., Pimelobacter sp. y Micrococcus sp. Se realizaron ensayos preliminares in vivo con los microorganismos escogidos y se inocularon diferentes combinaciones bacterianas en plántulas de encenillo y pagoda. Se evaluó el crecimiento longitudinal durante tres meses bajo condiciones de invernadero. Los mayores promedios se obtuvieron con la inoculación de Enterobacter gergoviae y Pantoea sp., en plántulas de encenillo (incremento promedio de 51% y 57% más que los controles sin ninguna inoculación microbiana y medio melaza estéril, respectivamente) y con la mezcla de todas las bacterias escogidas en pagoda (estimulación de 16% y 32%, con respecto a los controles melaza y sin inoculación bacteriana, respectivamente). Todas las combinaciones microbianas utilizadas estimularon significativamente el crecimiento longitudinal (prueba de Duncan P<0,05). El sinergismo mostrado por la co-inoculación de bacterias diazotróficas y solubilizadoras de fósforo indican el potencial de estos microorganismos como promotores de crecimiento vegetal y posibles biofertilizantes

BILROST: Handling Actuators of the Internet of Things through Tweets on Twitter using a Domain- Specific Language

In recent years, many investigations have appeared that combine the Internet of Things and Social Networks. Some of them addressed the interconnection of objects as Social Networks interconnect people, and others addressed the connection between objects and people. However, they usually used interfaces created for that purpose instead of using familiar interfaces for users. Why not integrate Smart Objects in traditional Social Networks? Why not control Smart Objects through natural interactions in Social Networks? The goal of this paper is to make easier to create applications that allow non-experts users to control Smart Objects actuators through Social Networks through the proposal of a novel approach to connect objects and people using Social Networks. This proposal will address how to use Twitter so that objects could perform actions based on Twitter users’ posts. Moreover, it will be presented a Domain-Specific language that could help in the task of defining the actions that objects could perform when people publish specific content on Twitter

A review about Smart Objects, Sensors, and Actuators

Smart Objects and the Internet of Things are two ideas which describe the future, walk together, and complement each other. Thus, the interconnection among objects can make them more intelligent or expand their intelligence to unsuspected limits. This could be achieved with a new network that interconnects each object around the world. However, to achieve this goal, the objects need a network that supports heterogeneous and ubiquitous objects, a network where exists more traffic among objects than among humans, but supporting for both types. For these reasons, both concepts are very close. Cities, houses, cars, machines, or any another object that can sense, respond, work, or make easier the lives of their owner. This is a part of the future, an immediate future. Notwithstanding, first of all, there are to resolve a series of problems. The most important problem is the heterogeneity of objects. This article is going to show a theoretical frame and the related work about Smart Object. The article will explain what are Smart Objects, doing emphasis in their difference with Not- Smart Objects. After, we will present one of the different object classification system, in our opinion, the most complete

Innovative acrylic thermoplastic composites versus conventional composites: Improving the impact performances

This study focuses on the benefits to the mechanical performance against impact loading offered by glass fiber reinforced (GFR) acrylic thermoplastic polymers, based on new room temperature cure methyl-methacrylate (MMA) matrix. Glass fiber reinforcement is a common solution for a wide variety of engineering applications based on thermoset matrices. However, its use presents some disadvantages such as adequate control of manufacturing temperature, problematic recycling and low damage tolerance. In contrast, acrylic polymers presents a high potential as an alternative matrix for thermoset composites due to their superior mechanical properties, manufacturing at low temperatures and recycled possibilities. In order to compare the mechanical behavior under impact loading of acrylic thermoplastic composites versus conventional composites, Charpy impact test and low velocity impact tests are carried out. The GFR acrylic laminate composites considered are compared to conventional composites manufactured with epoxy and polyester resins in terms of impact resistance and damage evolution. This study covers an impact energy rate from 10 to 60 J and analyses the maximum load, deflection, absorbed energy and associated damage, showing a better performance of the new GPR. acrylic thermoplastic polymers with respect to conventional GFR composites

Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.Fil: Schiaffarino, Olivia. Universidad Complutense de Madrid; EspañaFil: Valdivieso González, David. Hospital Universitario 12 de Octubre; España. Universidad Complutense de Madrid; EspañaFil: García Pérez, Inés M.. Universidad Complutense de Madrid; EspañaFil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur; ArgentinaFil: Almendro Vedia, Víctor G.. Universidad Complutense de Madrid; España. Hospital Doce de Octubre, Madrid; EspañaFil: Natale, Paolo. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; EspañaFil: López Montero, Iván. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; Españ

Minimized natural versions of fungal ribotoxins show improved active site plasticity

Fungal ribotoxins are highly specific extracellular RNases which cleave a single phosphodiester bond at the ribosomal sarcin-ricin loop, inhibiting protein biosynthesis by interfering with elongation factors. Most ribotoxins show high degree of conservation, with similar sizes and amino acid sequence identities above 85%. Only two exceptions are known: Hirsutellin A and anisoplin, produced by the entomopathogenic fungi Hirsutella thompsonii and Metarhizium anisopliae, respectively. Both proteins are similar but smaller than the other known ribotoxins (130 vs 150 amino acids), displaying only about 25% sequence identity with them. They can be considered minimized natural versions of their larger counterparts, best represented by α-sarcin. The conserved α-sarcin active site residue Tyr48 has been replaced by the geometrically equivalent Asp, present in the minimized ribotoxins, to produce and characterize the corresponding mutant. As a control, the inverse anisoplin mutant (D43Y) has been also studied. The results show how the smaller versions of ribotoxins represent an optimum compromise among conformational freedom, stability, specificity, and active-site plasticity which allow these toxic proteins to accommodate the characteristic abilities of ribotoxins into a shorter amino acid sequence and more stable structure of intermediate size between that of other nontoxic fungal RNases and previously known larger ribotoxins

EBD-ULL: una herramienta para la evaluación del rendimiento de sentencias SQL sobre diferentes sistemas gestores de bases de datos

La necesidad de evaluar el rendimiento de las sentencias SQL sobre diferentes gestores de bases de datos reales nos ha motivado a realizar una herramienta en la que los alumnos puedan comprobar empíricamente la teoría de la optimización de bases de datos. Las consultas, actualizaciones (inserciones, borrados,...) y demás instrucciones que los alumnos realizan en los problemas de clase, son facilitadas a la herramienta para comprobar su rendimiento. Las asignaturas de Bases de Datos y de Ingeniería del Software ofrecen al alumno la base conceptual necesaria para su utilización. La herramienta, desarrollada bajo la plataforma Java, permite evaluar no sólo diferentes sistemas gestores de bases de datos sino también diferentes arquitecturas

Thermoresponsive Polymeric Nanolenses Magnify the Thermal Sensitivity of Single Upconverting Nanoparticles

Lanthanide-based upconverting nanoparticles (UCNPs) are trustworthy workhorses in luminescent nanothermometry. The use of UCNPs-based nanothermometers has enabled the determination of the thermal properties of cell membranes and monitoring of in vivo thermal therapies in real time. However, UCNPs boast low thermal sensitivity and brightness, which, along with the difficulty in controlling individual UCNP remotely, make them less than ideal nanothermometers at the single-particle level. In this work, it is shown how these problems can be elegantly solved using a thermoresponsive polymeric coating. Upon decorating the surface of NaYF4:Er3+,Yb3+ UCNPs with poly(N-isopropylacrylamide) (PNIPAM), a >10-fold enhancement in optical forces is observed, allowing stable trapping and manipulation of a single UCNP in the physiological temperature range (20–45 °C). This optical force improvement is accompanied by a significant enhancement of the thermal sensitivity— a maximum value of 8% °C+1 at 32 °C induced by the collapse of PNIPAM. Numerical simulations reveal that the enhancement in thermal sensitivity mainly stems from the high-refractive-index polymeric coating that behaves as a nanolens of high numerical aperture. The results in this work demonstrate how UCNP nanothermometers can be further improved by an adequate surface decoration and open a new avenue toward highly sensitive single-particle nanothermometryThis work was supported by the Ministerio de Ciencia e Innovación de España (PID2019-106211RB-I00 PID2019-105195RA-I00 and MAT2017- 83111R), by the Comunidad de Madrid (S2017/BMD-3867 RENIM-CM), co-financed by European Structural and Investment Fund and by the Universidad Autónoma de Madrid and Comunidad Autónoma de Madrid (SI1/PJI/2019-00052 and PR38/21-36 ANTICIPA-CM). D.L. acknowledges a scholarship from the China Scholarship Council (201808350097). J.R.B. acknowledges the support from Carl Tryggers Foundation (CTS18:229). M.I.M acknowledges financial support from the Spanish Ministerio de Ciencia e Innovación, through the “María de Maeztu” Programme for Units of Excellence in R&D (CEX2018-000805-M) and the MELODIA PGC2018-095777-B-C22 proje

A fast model to resolve the velocity-space of fast-ion losses detected in ASDEX Upgrade and MAST Upgrade

A forward model to resolve the fast-ion loss velocity-space on a fast-ion loss detector (FILD) probe head (FILDSIM) has been extended, making it possible to perform real-time analysis of the FILD data ("real-time FILDSIM"). Parametric pre-processing with FILDSIM enables real-time mapping of the raw FILD measurements to the velocity-space of the fast-ion distribution reaching the FILD probe, which depends on the local magnetic field at the probe head. Such parametric pre-processing facilitates the study of fast-ion losses in stages of the discharge other than the flat-top, such as the ramp-up phase when changes in the local magnetic field at the probe head cannot be neglected. Real-time FILDSIM has been applied to the existing and newly installed FILDs in ASDEX Upgrade and will be used for the forthcoming FILD in MAST-Upgrade. Due to the larger size of the MAST-U FILD probe, the approximation used in FILDSIM of a uniform magnetic field in the FILD region has been generalised to the case of a non-uniform field, with gyro-orbits calculated numerically in this field.Universidad de Sevilla PP2016-7145Comunidad Europea de Energía Atómica (EURATOM) 633305

Receptores homodinos a 300 GHz basados en tecnología CMOS

Using CMOS transistors for terahertz detection is currently a disruptive technology that offers the direct integration of a terahertz detector with video preamplifiers. The detectors are based on the resistive mixer concept and its performance mainly depends on the following parameters: type of antenna, electrical parameters (gate to drain capacitor and channel length of the CMOS device) and foundry. Two different 300 GHz detectors are discussed: a single transistor detector with a broadband antenna and a differential pair driven by a resonant patch antenna