Diseño y construcción de un calorímetro de junkers para determinación del poder calorífico del biogás

Se presentan los primeros resultados obtenidos a través de un calorímetro de Junkers, diseñado y construido por el grupo G.I.D.E.R. (Grupo de Investigación y Desarrollo en Energías Renovables), utilizado para determinar el poder calorífico de gases. Este dispositivo consiste básicamente en un intercambiador de calor tipo serpentina vertical, de cobre, por la cual fluye agua en forma descendente, ubicada en el interior de un cilindro de acero donde circulan, en sentido contrario, los humos de la combustión. Primeramente se realizaron ensayos con un gas conocido como el butano con el fin de calibrar el aparato. Posteriormente se llevaron a cabo experiencias con metano resultando el poder calorífico del mismo 47341,2 Kj/Kg. Si bien los valores hallados en forma práctica difieren ligeramente de los extraídos de la bibliografía, los mismos se consideran satisfactorios, teniendo en cuenta que son los primeros resultados obtenidos con el prototipo construido.We present the first results obtained from a Junkers calorimeter, designed and built by the group G.I.D.E.R. (Research and Development Group on Renewable Energies), used to determine the calorific power of gases. This device is basically a vertical serpentine heat exchanger, of copper, water which flows downwards, located inside a steel cylinder, which circulate in the opposite direction, the smoke of combustion. First tests were performed with a gas known as butane to calibrate the device. Later experiments were conducted with methane resulting in a calorific power of 47341.2 KJ / KG. Although the values found in practically differ slightly from those from the literature, they are considered satisfactory, bearing in mind they are the first results obtained with the prototype built.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Diseño y construcción de un calorímetro de junkers para determinación del poder calorífico del biogás

Se presentan los primeros resultados obtenidos a través de un calorímetro de Junkers, diseñado y construido por el grupo G.I.D.E.R. (Grupo de Investigación y Desarrollo en Energías Renovables), utilizado para determinar el poder calorífico de gases. Este dispositivo consiste básicamente en un intercambiador de calor tipo serpentina vertical, de cobre, por la cual fluye agua en forma descendente, ubicada en el interior de un cilindro de acero donde circulan, en sentido contrario, los humos de la combustión. Primeramente se realizaron ensayos con un gas conocido como el butano con el fin de calibrar el aparato. Posteriormente se llevaron a cabo experiencias con metano resultando el poder calorífico del mismo 47341,2 Kj/Kg. Si bien los valores hallados en forma práctica difieren ligeramente de los extraídos de la bibliografía, los mismos se consideran satisfactorios, teniendo en cuenta que son los primeros resultados obtenidos con el prototipo construido.We present the first results obtained from a Junkers calorimeter, designed and built by the group G.I.D.E.R. (Research and Development Group on Renewable Energies), used to determine the calorific power of gases. This device is basically a vertical serpentine heat exchanger, of copper, water which flows downwards, located inside a steel cylinder, which circulate in the opposite direction, the smoke of combustion. First tests were performed with a gas known as butane to calibrate the device. Later experiments were conducted with methane resulting in a calorific power of 47341.2 KJ / KG. Although the values found in practically differ slightly from those from the literature, they are considered satisfactory, bearing in mind they are the first results obtained with the prototype built.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Dengue virus targets RBM10 deregulating host cell splicing and innate immune response

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact [email protected] experiments previously performed by our laboratories showed enrichment in intronic sequences and alterations in alternative splicing in dengue-infected human cells. The transcript of the SAT1 gene, of well-known antiviral action, displayed higher inclusion of exon 4 in infected cells, leading to an mRNA isoform that is degraded by non-sense mediated decay. SAT1 is a spermidine/spermine acetyl-transferase enzyme that decreases the reservoir of cellular polyamines, limiting viral replication. Delving into the molecular mechanism underlying SAT1 pre-mRNA splicing changes upon viral infection, we observed lower protein levels of RBM10, a splicing factor responsible for SAT1 exon 4 skipping. We found that the dengue polymerase NS5 interacts with RBM10 and its sole expression triggers RBM10 proteasome-mediated degradation. RBM10 over-expression in infected cells prevents SAT1 splicing changes and limits viral replication, while its knock-down enhances the splicing switch and also benefits viral replication, revealing an anti-viral role for RBM10. Consistently, RBM10 depletion attenuates expression of interferon and pro-inflammatory cytokines. In particular, we found that RBM10 interacts with viral RNA and RIG-I, and even promotes the ubiquitination of the latter, a crucial step for its activation. We propose RBM10 fulfills diverse pro-inflammatory, anti-viral tasks, besides its well-documented role in splicing regulation of apoptotic genes.Agencia Nacional de Promoción Científica y Tecnológica de Argentina (ANPCyT) [2014-2888, 2015-1731, 2017-0111 to A.S. and 2015-2555, 2017-1717 to A.V.G.]; Universidad de Buenos Aires, Argentina (UBACyT) [20020170100045BA to A.S.]; NIH (NIAID) [R01.AI095175 to A.V.G.]; Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET) [PIP 11220170100171CO to C.C.G]; B.P. has been a postdoctoral fellow from CONICET from 2017 to 2019 and is currently a postdoctoral fellow at the Institute of Cell Biology in the University of Bern, Switzerland; L.B. and M.E.G.S. are recipients of doctoral fellowships from CONICET; M.F.T. is a doctoral fellowship recipient from ANPCyT; N.G. has been an undergraduate fellowship recipient from the University of Buenos Aires (2018–2020); P.M. has been a doctoral fellow from CONICET (2015–2019) and is currently a postdoctoral fellow supported by H2020-Marie Sklodowska-Curie Research and Innovation Staff Exchanges [734825-LysoMod]; R.V.D. has been a visiting post-doctoral fellow at the Srebrow lab from IMM (Lisbon, Portugal) supported by the same program. A.S., A.V.G., C.C.G., N.G.I. and L.G.G. are career investigators from CONICET.info:eu-repo/semantics/publishedVersio

Provenance of Cambrian–Ordovician Siliciclastic Rocks of Southwestern Iberia: Insights into the Evolution of the North Gondwana Margin

This study makes a comparison between the populations of detrital zircons of the Cambrian sandstones from the Ossa–Morena Zone (OMZ) and the Ordovician quartzites from the southern domains of the Central Iberian Zone (S-CIZ) to identify the sediment sources during the development of North Gondwana basins (southwestern Iberia). The U–Pb results obtained for the lower Cambrian sandstones of the OMZ show a remarkable similarity to the detrital zircon ages of greywackes from the underlying OMZ Ediacaran basement (the Série Negra succession). However, there is a greater proportion of Cryogenian grains in the Cambrian rocks, whose main sources are: (1) the late Cadomian magmatic arcs (Ediacaran, ca. 635–545 Ma) which also contributed to filling the late Ediacaran basins of the OMZ; and (2) the early Cadomian arcs (Cryogenian, ca. 700–635 Ma). In the Lower Ordovician quartzites of the S-CIZ (the Armorican and Sarnelha formations), the age distribution of detrital zircons overlaps the population of detrital zircons of the underlying S-CIZ Ediacaran basement (the Beiras Group). However, there are some differences in the Sarnelhas quartzites, which have a population of detrital zircons similar to those of the Ediacaran greywackes and Cambrian sandstones of the OMZ. The Cambrian grains found in the Lower Ordovician quartzites fit the ages of magmatism representing the onset of rifting in North Gondwana that is registered in the OMZ but absent from the S-CIZ. The early Ordovician zircon grains are probably related to the magmatic event that preceded the passive margin stage of the Rheic Ocean, and are found in both the CIZ and OMZ

Prognostic value of hematogenous dissemination and biological profile of the tumor in early breast cancer patients: A prospective observational study

<p>Abstract</p> <p>Background</p> <p>The aim of this study was to investigate the incidence and prognostic value of disseminated tumor cells in bone marrow of breast carcinoma patients with early disease, and to analyze this finding in relation to lymph node involvement, determined by sentinel lymph node (SLN) biopsy analysis, and to prognostic factors of interest.</p> <p>Methods</p> <p>104 patients with operable (T < 3 cm) breast cancer and clinically- and sonographically-negative axillary lymph nodes were scheduled for SLN biopsy. Bone marrow aspirates were collected before the start of surgery from both iliac crests, and mononuclear cell layers were separated by density centrifugation (Lymphoprep). Slide preparations were then examined for the presence of disseminated tumor cells by immunocytochemistry with anti-cytokeratin antibodies (A45-B/B3). Lymphoscintigraphy was performed 2 hours after intratumor administration of 2 mCi (74 MBq) of 99mTc colloidal albumin. The SLN was evaluated for the presence of tumor cells by hematoxylin-eosin staining and, when negative, by immunocytochemistry using anti-cytokeratin antibody (CAM 5.2). Survival analyses and comparative analyses were performed on the results of bone marrow determinations, SLN biopsy, and known prognostic factors, including breast cancer subtypes according to the simplified classification based on ER, PR and HER2.</p> <p>Results</p> <p>Lymph node and hematogenous dissemination occur in one-third of patients with early-stage breast cancer, although not necessarily simultaneously. In our study, disseminated tumor cells were identified in 22% of bone marrow aspirates, whereas 28% of patients had axillary lymph node involvement. Simultaneous lymph node and bone marrow involvement was found in only 5 patients (nonsignificant). In the survival study (60 months), a higher, although nonsignificant rate of disease-related events (13%) was seen in patients with disseminated tumor cells in bone marrow, and a significant association of events was documented with the known, more aggressive tumor subtypes: triple negative receptor status (21%) and positive ERBB2 status (29%).</p> <p>Conclusions</p> <p>Tumor cell detection in bone marrow can be considered a valid prognostic parameter in patients with early disease. However, the classic prognostic factors remain highly relevant, and the newer breast cancer subtypes are also useful for this purpose.</p

Measuring socio-demographic differences in volunteers with a value-based index: illustration in a mega event

The phenomenon of volunteering can be analysed as a consumer experience through the concept of value as a trade-off between benefits and costs. In event volunteering, both the expected value (pre-experienced) and the perceived value (post-experienced) of volunteering can be assessed. With this purpose, an online quantitative survey is conducted with a sample of 711 volunteers in a religious mega event, with questions related to five dimensions of their experience: efficiency, social value, play, spirituality and time spent. These five scales, properly tested are used for building a multidimensional index of both the expected and perceived value of the volunteer experience. ANOVAs test show significant differences on the index in both moments upon the socio-demographic profiles: negative expectations/experience balance by age, contrasted results by sex, and more experienced volunteers being more critical with the value experienced. Implications for event managers are proposed, in line with the motivation of volunteers

Non-motor symptom burden in patients with Parkinson's disease with impulse control disorders and compulsive behaviours : results from the COPPADIS cohort

The study was aimed at analysing the frequency of impulse control disorders (ICDs) and compulsive behaviours (CBs) in patients with Parkinson's disease (PD) and in control subjects (CS) as well as the relationship between ICDs/CBs and motor, nonmotor features and dopaminergic treatment in PD patients. Data came from COPPADIS-2015, an observational, descriptive, nationwide (Spain) study. We used the validated Questionnaire for Impulsive-Compulsive Disorders in Parkinson's Disease-Rating Scale (QUIP-RS) for ICD/CB screening. The association between demographic data and ICDs/CBs was analyzed in both groups. In PD, this relationship was evaluated using clinical features and treatment-related data. As result, 613 PD patients (mean age 62.47 ± 9.09 years, 59.87% men) and 179 CS (mean age 60.84 ± 8.33 years, 47.48% men) were included. ICDs and CBs were more frequent in PD (ICDs 12.7% vs. 1.6%, p < 0.001; CBs 7.18% vs. 1.67%, p = 0.01). PD patients had more frequent previous ICDs history, premorbid impulsive personality and antidepressant treatment (p < 0.05) compared with CS. In PD, patients with ICDs/CBs presented younger age at disease onset, more frequent history of previous ICDs and premorbid personality (p < 0.05), as well as higher comorbidity with nonmotor symptoms, including depression and poor quality of life. Treatment with dopamine agonists increased the risk of ICDs/CBs, being dose dependent (p < 0.05). As conclusions, ICDs and CBs were more frequent in patients with PD than in CS. More nonmotor symptoms were present in patients with PD who had ICDs/CBs compared with those without. Dopamine agonists have a prominent effect on ICDs/CBs, which could be influenced by dose

Anales del III Congreso Internacional de Vivienda y Ciudad "Debate en torno a la nueva agenda urbana"

Acta de congresoEl III Congreso Internacional de Vivienda y Ciudad “Debates en torno a la NUEVa Agenda Urbana”, ha sido una apuesta de alto compromiso por acercar los debates centrales y urgentes que tensionan el pleno ejercicio del derecho a la ciudad. Para ello las instituciones organizadoras (INVIHAB –Instituto de Investigación de Vivienda y Hábitat y MGyDH-Maestría en Gestión y Desarrollo Habitacional-1), hemos convidado un espacio que se concretó con potencia en un debate transdisciplinario. Convocó a intelectuales de prestigio internacional, investigadores, académicos y gestores estatales, y en una metodología de innovación articuló las voces académicas con las de las organizaciones sociales y/o barriales en el Foro de las Organizaciones Sociales que tuvo su espacio propio para dar voz a quienes están trabajando en los desafíos para garantizar los derechos a la vivienda y los bienes urbanos en nuestras ciudades del Siglo XXI

A non-coding RNA network involved in KSHV tumorigenesis

Regulatory pathways involving non-coding RNAs (ncRNAs) such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA) have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors, we have analyzed the host and viral transcriptome to uncover the role lncRNA-miRNA-mRNA driven networks in KSHV tumorigenesis. The integration of the differentially expressed ncRNAs, with an exhaustive computational analysis of their experimentally supported targets, led us to dissect complex networks integrated by the cancer-related lncRNAs Malat, Neat1, H19, Meg3 and their associated miRNA-target pairs. These networks would modulate pathways related to KSHV pathogenesis, such as viral carcinogenesis, p53 signaling, RNA surveillance, and Cell cycle control. Finally, the ncRNA-mRNA analysis allowed us to develop signatures that can be used to an appropriate identification of druggable gene or networks defining relevant AIDS-KS therapeutic targets.Centro de Investigaciones Inmunológicas Básicas y Aplicada