El periodismo de moda a través de Instagram. Análisis de las cuentas de Telva, Vogue y Harper’s Bazaar España

El presente trabajo supone un recorrido desde los inicios del periodismo de moda en el siglo XVII hasta nuestros días. Sumado a esto, hemos llevado a cabo un análisis de contenido de las cuentas oficiales de Instagram de las tres principales revistas de moda en España: Telva, Harper’s Bazaar España y Vogue España.Universidad de Sevilla. Grado en Periodism

Geminivirus C2 protein represses genes involved in sulphur assimilation and this effect can be counteracted by jasmonate treatment

Geminiviruses are plant viruses that infect a broad range of crops and cause extensive losses worldwide, having an important economic impact. C2, a multifunctional pathogenicity factor encoded by geminiviruses, has been recently shown to suppress the responses to jasmonates in the host plant, which might at least partially explain its well-established role in pathogenicity. Sulphur is one of the essential macro-elements for plant life, and is considered to have a role in plant defence, in a phenomenon named sulphur-induced resistance (SIR) or sulphur-enhanced defence (SED). In this work, we show that geminivirus C2 protein represses the expression of genes involved in the sulphur assimilation pathway in Arabidopsis, but, interestingly, this effect can be neutralized by exogenous jasmonate treatment. These preliminary results may raise the idea that geminiviruses might be affecting sulphur metabolism, and maybe counteracting SIR/SED, through the manipulation of the jasmonate signalling pathway, which would define a novel strategy in plant-virus interactions and may unveil SIR/SED as an important player in the plant defence against viruses.Ministerio de Ciencia y Innovación/FEDER AGL2007-66062-C02-02/AGR AGL2010-22287-CO2European Regional Development Fund (ERDF) BIO2010-15201Junta de Andalucía BIO­27

Peripheral Inflammation Enhances Microglia Response and Nigral Dopaminergic Cell Death in an in vivo MPTP Model of Parkinson’s Disease

The impact of systemic inflammation in nigral dopaminergic cell loss remains unclear. Here, we have investigated the role of peripheral inflammation induced by systemic lipopolysaccharide (LPS) administration in the MPTP-based model of Parkinson’s disease. Brain inflammation, microglia and astroglia activation, disruption of the blood–brain barrier (BBB) and integrity of the nigrostriatal dopaminergic system were evaluated in response to i.p. injection of LPS, MPTP or the combination of both. Our results showed that combinative treatment exacerbates microglia activation and enhances (i) the appearance of galectin-3-positive microglia, recently identified as microglial disease-associated phenotypic marker, (ii) the up-regulation of pro-inflammatory cytokines, (iii) the occurrence of A1 neurotoxic astrocytes, (iv) the breakdown of the BBB, and (v) the loss of dopaminergic neurons in the substantia nigra. Microglia activation was triggered earlier than other degenerative events, suggesting that over-activation of microglia (including different polarization states) may induce dopaminergic neuron loss by itself, initiating the endless cycle of inflammation/degeneration. Our study revitalizes the importance of peripheral inflammation as a potential risk factor for Parkinson’s disease and raises the possibility of using new anti-inflammatory therapies to improve the course of neurodegenerative diseases, including those directly aimed at modulating the deleterious activity of disease-associated microglia.España MINECO y FEDER SAF2015-64171-

Formation of stimuli-responsive cyclophanes by self-assembly: the case of carbazole-based biradicals

Dynamic covalent bonds has recently received lot of attention because of their unique feature to become reversible under mild conditions.[1] In this context, π-conjugated biradical compounds has emerged as essential building blocks.[2] For instance, we have demonstrated that 2,7-dicyanomethylene-9-(2-ethylhexyl)carbazole biradical reversibly converts to a macrocycle cyclophane upon soft stimuli (temperature, pressure, light), showing strong chromic effects.[3] We now extent this study towards longer conjugated carbazole backbone (i.e., indolocarbazole shown in Figure 1), aiming at investigating how the elongation of the conjugated backbone impacts on the formation of stimuli-responsive cyclophanes. The self-assembly process is investigated both in solution and solid state by linking theory and experiments.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Causes of Non-Vaccination Against Covid-19 in Workers at a Spanish Hospital

Articulo original[ES] Introducción: En Ceuta, el 82,3% de la población ha recibido al menos una vacuna contra la COVID-19. El 17,7% es reticente a recibir ninguna vacuna relacionada con esta enfermedad. El objetivo de este estudio es conocer el grado de rechazo y las causas del mismo hacia la vacunación contra la COVID-19 en trabajadores del ámbito sanitario de Hospital Universitario de Ceuta; además, poder comparar estos resultados con las causas en diferentes países y con otros sanitarios del mundo. Método: Se realizó un estudio descriptivo transversal donde se revisó la base de datos de los trabajadores excluyen-do aquellos que sí habían recibido al menos una dosis de la vacuna. En aquellos donde no se tenía información o bien no habían recibido vacunación, se realizó una llamada telefónica. Se diseñó una base de datos con diferentes variables entre las que se incluyen: categoría profesional, sexo y edad. El análisis de los datos se llevó a cabo con Excel 2010.Resultados: De los 1009 trabajadores registrados, 17 de ellos confirmó no haber recibido ninguna vacuna: 6 enfermeros (35%), 6 médicos (35%), 3 administrativos (18%), 1 celador (6%) y 1 auxiliar de enfermería (6%). 35% lo atribuyó a causas médicas, 35% se encontraron reticentes a recibir la vacuna y 18% refirió tener anticuerpos contra el virus y por lo tanto, no deseó recibir la vacunación. Conclusiones: La causa más frecuente de no vacunación es el miedo (35%) tanto a los efectos secundarios como a la rapidez del desarrollo de la vacuna. [EN] Introduction: In Ceuta, 82.3% of the population has received at least one vaccine against COVID-19. 17.7% reject to receive any vaccine related to this disease. The objective of this study is to determine the degree of rejection and its causes towards vaccination against COVID-19 in health workers of the Hospital Universitario de Ceuta; In addition, to be able to compare these results with the causes in different countries and with other healthcare providers in the world. Method: A cross-sectional descriptive study was carried out where the database of workers was reviewed, exclud-ing those who had received at least one dose of the vaccine. In those where there was no information or had not received vaccination, a telephone call was made. A database was designed with different variables, including: pro-fessional category, sex and age. Data analysis was carried out with Excel 2010. Results: Of the 1,009 registered workers, 17 of them confirmed that they had not received any vaccine: 6 nurses (35%), 6 doctors (35%), 3 administrative staff (18%), 1 porter (6%), and 1 nursing assistant (6 %). 35% attributed it to medical causes, 35% were reluctant to receive the vaccine and 18% reported having antibodies against the virus and therefore did not want to receive the vaccination. Conclusions: The most frequent cause of non-vaccination is fear (35%) of both side effects and the speed of vaccine developmentN

Carbazole-based Diradicals for Dynamic Covalent Chemistry

Dynamic covalent chemistry (DCC) is focused on the creation of structural scaffolds based on chemical components that interact through strong but reversible bonds. In fact, dynamic covalent bonds receive lot of attention because of their unique feature to become reversible under mild conditions.1 conjugated diradical compounds has emerged as essential building blocks in DCC.2 In this work, we will review our most recent works on the formation of stimuli-responsive cyclophanes by self-assembly of carbazole-based diradicals. To this end, we use a combined experimental and theoretical approach that links vibrational spectroscopy with DFT calculations. In this sense, it is interesting to note that we have recently demonstrated the potential of a para-substituted carbazole with terminal dicyanomethylene (DCM) groups to act as building blocks in DCC.3 This quinoid carbazole monomer transforms to a macrocycle cyclophane upon soft external stimuli (temperature, pressure, light), showing strong chromic features. In addition, we have also recently explored how the different DCM substitution position affects the interesting chromoactive properties of carbazole compounds.4 Finally, we are currently exploring the effect of the elongation of the carbazole backbone on the formation of stimuli-responsive cyclophanes.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Multiresponsive chromic soft materials: formation of strongly coupled σ-dimers from IndoloCarbazole-based biradicaloids

Dynamic covalent chemistry is focused on the creation of structural scaffolds based on chemical components that interact through strong but reversible bonds. In fact, dynamic covalent bonds receive lot of attention because of their unique feature to become reversible under mild conditions.1 π-conjugated biradical compounds has emerged as essential building blocks in DCC (dynamic covalent chemistry).2 We have recently demonstrated the potential of a para-substituted carbazole with terminal dicyanomethylene groups to act as building blocks in DCC.3 In fact, this quinoid carbazole monomer transform to a macrocycle cyclophane upon soft external stimuli (temperature, pressure, light), showing strong chromic features. Here, we explore the effect of the elongation of the carbazole backbone on the formation of stimuli-responsive cyclophanes by self-assembly. To this end, we use a join experimental and theoretical approach that links vibrational spectroscopy (Raman and IR) with DFT calculationsUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Differential glycosylation and extracellular vesicle biogenesis in HEK293 upon transient transfection

Vaccine therapies based on virus-like particles (VLPs) are currently increasing relevance due to the strong immune response they elicit and their manufacture advantages when compared to traditional biopharmaceuticals. During VLP production using mammalian cell-based platforms, different extracellular vesicles (EVs) are coproduced, leading to the need of a complex downstream purification process. Currently there is no effective and efficient method to separate VLPs from EVs which share very similar density and physicochemical properties1,2. Different methods to characterize the EV composition and their protein content and glycosylation signature are used in this work to further understand their biochemical nature. First, a sucrose cushion ultracentrifugation was carried out to isolate the VLP fraction, also containing co-purified EVs. Following, a multiplexed quantitative proteomic approach was used to characterize the VLP-copurified secretome. Three conditions were studied, a non-transfected condition, transiently transfected with an empty plasmid (mock) and with a plasmid for Gag VLP production. Please click Download on the upper right corner to see the full abstract

Assessment of the sensitivity and specificity of serological (IFAT) and molecular (direct PCR) techniques for diagnosis of leishmaniasis in lagomorphs using a Bayesian approach

Leishmaniasis, caused by Leishmania infantum, is a vector-borne zoonotic disease that is endemic to the Mediterranean basin. The potential of rabbits and hares to serve as competent reservoirs for the disease has recently been demonstrated, although assessment of the importance of their role on disease dynamics is hampered by the absence of quantitative knowledge on the accuracy of diagnostic techniques in these species. A Bayesian latent-class model was used here to estimate the sensitivity and specificity of the Immuno-fluorescence antibody test (IFAT) in serum and a Leishmania-nested PCR (Ln-PCR) in skin for samples collected from 217 rabbits and 70 hares from two different populations in the region of Madrid, Spain. A two-population model, assuming conditional independence between test results and incorporating prior information on the performance of the tests in other animal species obtained from the literature, was used. Two alternative cut-off values were assumed for the interpretation of the IFAT results: 1/50 for conservative and 1/25 for sensitive interpretation. Results suggest that sensitivity and specificity of the IFAT were around 70–80%, whereas the Ln-PCR was highly specific (96%) but had a limited sensitivity (28.9% applying the conservative interpretation and 21.3% with the sensitive one). Prevalence was higher in the rabbit population (50.5% and 72.6%, for the conservative and sensitive interpretation, respectively) than in hares (6.7% and 13.2%). Our results demonstrate that the IFAT may be a useful screening tool for diagnosis of leishmaniasis in rabbits and hares. These results will help to design and implement surveillance programmes in wild species, with the ultimate objective of early detecting and preventing incursions of the disease into domestic and human populations