Aspectos da disciplina geral da multipropriedade imobiliária

O presente trabalho tem por objetivo a análise da disciplina da Multipropriedade irradiada pela Lei 13.777/2018, com reflexos no código civil de 2002 e na Lei de Registros Públicos. Esta análise ocorre baseada no conceito de Time Sharing definido como um parcelamento temporal do Imóvel. Serão analisadas as divergências doutrinárias presentes no período que antecede à implementação da Lei. Além disso pretende observar os institutos da Lei, mais especificamente as disposições gerais, a instituição da multipropriedade e os direitos e obrigações do multiproprietário. Por fim, pretende se analisar o cabimento e forma de aplicação de uma multipropriedade sobre bens móveis

Pré-qualificação de fornecedores e padronização de marcas na Saúde Pública Brasileira:: um estudo exploratório

O objetivo desse artigo é realizar uma análise exploratória de modelos de pré-qualificação e padronização de marcas em unidades de saúde pública no Brasil. Para isso, foi realizado um estudo de caso múltiplo, em três instituições públicas da esfera federal do setor de saúde, para identificação de semelhanças e diferenças entre os procedimentos adotados, através da realização de entrevistas semiestruturadas e análise documental. Entre os resultados encontrados, pode-se destacar i) a existência de uma análise documental, tanto do ponto vista da regularidade cadastral da empresa quanto do ponto de vista da regularidade sanitária do produto; ii) uma análise técnica de amostras e das condições de fabricação do fornecedor; iii) a forte relação entre esses processos e as ações de pré e pós-mercado da Agência Nacional de Vigilância Sanitária, iv) a formalização de unidades organizacionais dedicadas a essa atividade e v) as formas distintas de entendimento das organizações quanto aos conceitos de pré-qualificação de fornecedores e padronização de marcas

Lymphotoxin-β Receptor Signaling through NF-κB2-RelB Pathway Reprograms Adipocyte Precursors as Lymph Node Stromal Cells

SummaryLymph node development during embryogenesis involves lymphotoxin-β receptor engagement and subsequent differentiation of a poorly defined population of mesenchymal cells into lymphoid tissue organizer cells. Here, we showed that embryonic mesenchymal cells with characteristics of adipocyte precursors present in the microenvironment of lymph nodes gave rise to lymph node organizer cells. Signaling through the lymphotoxin-β receptor controlled the fate of adipocyte precursor cells by blocking adipogenesis and instead promoting lymphoid tissue stromal cell differentiation. This effect involved activation of the NF-κB2-RelB signaling pathway and inhibition of the expression of the key adipogenic factors Pparγ and Cebpα. In vivo organogenesis assays show that embryonic and adult adipocyte precursor cells can migrate into newborn lymph nodes and differentiate into a variety of lymph node stromal cells. Thus, we propose that adipose tissues act as a source of lymphoid stroma for lymph nodes and other lymphoid structures associated with fat

Defective lymphoid organogenesis underlies the immune deficiency caused by a heterozygous S32I mutation in IκBα.

Patients with ectodermal dysplasia with immunodeficiency (ED-ID) caused by mutations in the inhibitor of NF-κB α (IκBα) are susceptible to severe recurrent infections, despite normal T and B cell numbers and intact in vitro lymphocyte function. Moreover, the outcome of hematopoietic stem cell transplantation (HSCT) in these patients is poor despite good engraftment. Mice heterozygous for the IκBα S32I mutation found in patients exhibited typical features of ED-ID. Strikingly, the mice lacked lymph nodes, Peyer's patches, splenic marginal zones, and follicular dendritic cells and failed to develop contact hypersensitivity (CHS) or form germinal centers (GCs), all features not previously recognized in patients and typical of defective noncanonical NF-κB signaling. Lymphotoxin β receptor (LTβR)-driven induction of chemokines and adhesion molecules mediated by both canonical and noncanonical NF-κB pathways was impaired, and levels of p100 were markedly diminished in the mutant. IκBα mutant → Rag2(-/-), but not WT→IκBα mutant, bone marrow chimeras formed proper lymphoid organs and developed CHS and GCs. Defective architectural cell function explains the immunodeficiency and poor outcome of HSCT in patients with IκBα deficiency and suggests that correction of this niche is critical for reconstituting their immune function

In vitro decidualisation of canine uterine stromal cells

BACKGROUND: The uterine response to the presence of embryos is poorly understood in the domestic dog (Canis familiaris). The intimate embryo-maternal cross-talk, which begins following the hatching of blastocysts and embryo attachment leads to strong structural and functional remodelling of the uterus. A part of this process is decidualisation, comprising morphological and biochemical changes that result in formation of maternal stroma-derived decidual cells. These are an integral part of the canine placenta materna, which together with the maternal vascular endothelium are the only cells of the canine endotheliochorial placenta able to resist trophoblast invasion. These cells are also the only ones within the canine placenta expressing the progesterone receptor (PGR). Understanding the decidualisation process thus appears essential for understanding canine reproductive physiology. METHODS: Here, we investigated the capability of canine uterine stromal cells to decidualise in vitro, thereby serving as a canine model of decidualisation. A dbcAMP-mediated approach was chosen during a time course of 24 - 72 h. Tissue material from six (n = 6) healthy, dioestric bitches was used (approximately 2 weeks after ovulation). Cells were characterized by differential staining, nearly 100 % of which were vimentin-positive. Scanning and transmission electron microscope analyses were applied, and morphological changes were recorded with a live cell imaging microscope. Expression of several decidualisation markers was investigated. RESULTS: The in vitro cultured stromal cells acquired characteristics of decidual cells when incubated with 0.5 mM dbcAMP for 72 h. Their shape changed from elongated to rounded, while ultrastructural analysis revealed higher numbers of mitochondria and secretory follicles, and an increased proliferation rate. Elevated expression levels of IGF1, IGF2, PRLR and ERα were observed in decidualised cells; PRL and ERβ remained mostly below the detection limit, while PGR remained unaffected. The expression of smooth muscle α actin (αSMA), another decidualisation marker, was strongly induced. Among prostaglandin system members, levels of COX2 (PTGS2) and of PGE2-synthase (PTGES) were upregulated. Expression of the PGE2 receptors, PTGER2 and PTGER4, was clearly detectable. CONCLUSION: An in vitro decidualisation model with canine uterine stromal cells was successfully established, allowing future, more detailed studies to be undertaken on the underlying molecular and endocrine mechanisms of canine decidualisation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12958-015-0066-4) contains supplementary material, which is available to authorized users

Biology and Signal Transduction pathways of the Lymphotoxin-αβ/LTβR system

This review focuses on the biological functions and signalling pathways activated by Lymphotoxin α (LTα)/Lymphotoxin β (LTβ) and their receptor LTβR. Genetic mouse models shed light on crucial roles for LT/LTβR to build and to maintain the architecture of lymphoid organs and to ensure an adapted immune response against invading pathogens. However, chronic inflammation, autoimmunity, cell death or cancer development are disorders that occur when the LT/LTβR system is twisted. Biological inhibitors, such as antagonist antibodies or decoy receptors, have been developed and used in clinical trials for diseases associated to the LT/LTβR system. Recent progress in the understanding of cellular trafficking and NF-κB signaling pathways downstream of LTα/LTβ may bring new opportunities to develop therapeutics that target the pathological functions of these cytokines

BMI-associated gene variants in FTO and cardiometabolic and brain disease: obesity or pleiotropy?

Pathophysiology, epidemiology and therapy of agein

Estruturação e análise de viabilidade de uma camiseteria voltada ao mercado de eventos universitários do Rio de Janeiro

O mercado de entretenimento universitário, em especial os eventos focados em disputas esportivas, apresenta crescimento significativo e grandes oportunidades de atuação sobre o ponto de vista empreendedor. Sendo assim, os autores deste estudo buscam entender e contextualizar o mercado de jogos universitários do Rio de Janeiro, a fim de propor um empreendimento que explore as demandas reprimidas deste mercado ainda em estágio inicial de desenvolvimento. Este trabalho utiliza a ferramenta Business Model Canvas e indicadores clássicos de análise de investimentos para apresentar e avaliar o empreendimento proposto