CYTOTOXICITY EVALUATION OF CARBON NANOMATERIALS ON HUMAN CELL LINES USING MTT ASSAY

Objective: The aim of this study was to evaluate and compare the in vitro toxicity of three carbon nano particles on five different cell lines. Methods: Human alveolar epithelial (A549) cells, hepatocytes (Hep G2 cells), human embryonic kidney cells, HCT 116, and intestinal (P407 cells) cells were exposed to multi walled carbon nanotubes, carbon nano fibres and carbon nano rods. The adverse effects of carbon nano particles were analyzed after 48 h incubation with different cell lines using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: Incubation of carbon nano particles with different cells produced a concentration-dependent inhibition of growth of the cells. The TC50 values (toxic concentration 50, i. e., concentration of particles inducing 50% cell mortality) of three nano particles was found to be in the range 28.29–46.35 µg/mL, and less than that of quartz (known toxic agent, 30.24-54.95 µg/mL). Conclusion: The results indicating the greater cytotoxic effect of carbon nano particles than quartz particles

CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES

Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colon (Caco-2) cell cultures in relevance to human risk assessmentMethods: The different concentrations of test TNP 20 and ZNP 20 1-300 µg/ml were exposed to determine the cell viability reduction on four human cell lines after 48 h post exposure using 3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mitochondrial membrane activities of the viable cells were determined with intensity of formazon formation by interpreting ELISA absorbance values at 470 nm.Results: The percent of cytotoxicity was determined by comparing percentage of cell viability reduction of test with that of control. The ZNP 20 produced higher cytotoxicity at the doses 100 (p<0.05) and 300 (p<0.001) µg/ml significantly on tested four human skin (HaCaT), lung (A549), liver (Hep G2) and colon (Caco-2) cells compared to TNP 20. The tested NP induced lesser cytotoxicity at lower concentrations with 1 and 3µg/ml in all the tested four cell lines. The induced cytotoxicity was an indicator for increased intracellular reactive oxygen species which further cause's major cell damage and cell death.Conclusion: The tested NP were induced greater cytotoxicity in the colon, Liver, lung and skin cells at higher concentrations 100 and 300 µg/ml significantly. The cytotoxicity order of TNP 20 and ZNP 20 at the highest dose (300µg/ml) were concluded as Caco-2>Hep G2>A549>HaCaT for 48 h post exposed cells

Zn(II), Cd(II) and Hg(II) metal complexes of 2-aminonicotinaldehyde: Synthesis, crystal structure, biological evaluation and molecular docking study

In this report, the ligand, 2-aminonicotinaldehyde (ANA) and its metal complexes Zn(ANA)(2)Cl-2], Cd (ANA)(2)Cl-2] and Hg(ANA)(2)Cl-2] have been synthesized and characterized by analytical and various spectroscopic (IR, Electronic, H-1 and C-13 NMR) studies. The single crystal X-ray diffraction studies of ANA and Zn(ANA)(2)Cl-2] complex have been discussed. It was found that intra/intermolecular hydrogen bonding exists in both compounds and N-atom of pyridine ring of ANA coordinated to the metal ion in tetrahedral fashion. Metal chlorides, ANA and its complexes were subjected to biological screening, antioxidant, in vitro cytotoxicity and EGFR (Epidermal growth factor receptor) targeting molecular docking studies which indicated the synergistic effect of metal complexes on the biological activity than the free ligand and metal chlorides. (C) 2017 Elsevier B.V. All rights reserved