Enzyme activities in aged conidia of N. crassa

Enzyme activities in aged conidia of N. crass

Ecohydrology: processes and implications for rangelands

This chapter is organized around the concept of ecohydrological processes that are explicitly tied to ecosystem services. Ecosystem services are benefits that people receive from ecosystems. We focus on (1) the regulating services of water distribution, water purification, and climate regulation; (2) the supporting services of water and nutrient cycling and soil protection and restoration; and (3) the provisioning services of water supply and biomass production. Regulating services are determined at the first critical juncture of the water cycle—on the soil surface, where water either infiltrates or becomes overland flow. Soil infiltrability is influenced by vegetation, grazing intensity, brush management, fire patterns, condition of biological soil crusts, and activity by fauna. At larger scales, water-regulating services are influenced by other factors, such as the nature and structure of riparian zones and the presence of shallow groundwater aquifers. Provisioning services are those goods or products that are directly produced from ecosystems, such as water, food, and fiber. Work over the last several decades has largely overturned the notion that water supply can be substantially increased by removal of shrubs. In riparian areas, surprisingly, removal of invasive, non-native woody plants appears to hold little potential for increasing water supply. Here, the primary factor appears to be that non-native plants use no more water than the native vegetation they displace. Clearly there is a close coupling between biota (both fauna and flora) and water on rangelands—which is why water-related ecosystem services are so strongly dependent on land management strategies.Fil: Wilcox, Bradford P.. Texas A&M University; Estados UnidosFil: Le Maitre, David. Council for Scientific and Industrial Research; SudáfricaFil: Jobbagy Gampel, Esteban Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; ArgentinaFil: Wang, Lixin. Indiana University; Estados UnidosFil: Breshears, David D.. University of Arizona; Estados Unido

Distribución espacial y controles ambientales de las represas (tajamares) en el Chaco Árido

Las regiones áridas tienen déficit hídrico a lo largo de todo el año, lo cual limita el crecimiento de la vegetación y la provisión de agua para bebida animal. El Chaco Árido (~10 Mha) sostiene una producción ganadera extensiva de baja inversión, basada en cosechar agua de lluvia en represas (tajamares) como principal fuente de abastecimiento de agua. En este trabajo se determinó la distribución espacial de las represas y su relación con el entorno biofísico (precipitación, vegetación, caminos) en el Chaco Árido. Para ello analizamos imágenes satelitales, información vectorial y análisis multivariados sobre una grilla de 135 celdas de 0.25°x0.25° cada una. En total identificamos 7920 represas (1 cada 1230 ha) y observamos densidades máximas de 1 represa cada 185 ha, pero también celdas sin represas, asociadas a la presencia de sierras, salinas y dunas. La densidad de represas fue mayor en celdas con mayor densidad de establecimientos ganaderos, caminos y carga animal (r=0.63, r=0.56 y r=0.51, respectivamente; P<0.01 en todos los casos), y en sitios con mayor precipitación media anual y menor variabilidad interanual (r=0.62 y r=-0.47, respectivamente; P<0.01 para ambos casos). Aunque la precipitación media anual fue el atributo que mejor se asoció a la distribución de las represas a escala regional, dicha relación fue más variable hacia los extremos del gradiente (árido y subhúmedo). Esto puede deberse a factores antrópicos tales como la baja rentabilidad de los sistemas ganaderos y la historia o el cambio en el uso del suelo. Los resultados de este trabajo representan un primer intento para dimensionar la importancia que tienen las represas en el Chaco Árido. Consideramos que este estudio puede ser útil para entender la producción ganadera y también para futuras investigaciones relacionadas con la conservación de la vida silvestre y el desarrollo de poblados rurales en la región.Spatial distribution and environmental controls of dams (small impoundments) in the Arid Chaco. Arid regions are characterized by water shortage throughout the year, which constrains both the vegetation growth and the supply of water for livestock consumption. The Arid Chaco (~10 Mha) hosts extensive and low-investment livestock systems based on rainwater harvesting stored in dams (small impoundments) as the main source of water supply. In this study, we characterized the spatial distribution of dams in the Arid Chaco and analyzed their relationship with biophysical variables (e.g. rainfall, vegetation, roads). For these purposes, we used satellite images, vector information and performed multivariate analysis on a spatial grid of 135 cells of 0.25°x0.25°. In total, 7920 dams were identified (1 dam every 1230 ha), with maximum densities of 1 dam every 185 ha but also cells without dams associated with the presence of mountains, salt flats and dunes. Dam density was higher in cells with higher densities of livestock establishments, roads and cale stock (r=0.63, r=0.56 and r=0.51, respectively; P<0.01 in all cases), and in cells with higher mean annual rainfall and lower interannual rainfall variability (r=0.62 and r=-0.47, respectively; P<0.01 for both cases). Although the mean annual rainfall was the best-associated variable with the distribution of dams at the regional scale, this relationship weakened towards the extremes of the gradient (arid and sub-humid). This may be due to anthropic factors such as the low profitability of livestock systems, the previous land uses or land-use changes. The results of this study represent a first aempt to assess the importance of impoundments in the Arid Chaco. We believe that this study may be useful not only to understand livestock production, but also for future studies related to the conservation of wildlife and the development of rural towns in the region.Instituto de Investigación Animal del Chaco SemiáridoFil: Niborski, Marcos J. Universidad Nacional de San Luis. Grupo de Estudios Ambientales-IMASL. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Niborski, Marcos J. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Manejo y Conservación de Suelos; ArgentinaFil: Murray, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Luis. Agencia De Extensión Rural San Luis; ArgentinaFil: Jobbágy, Esteban G. Universidad Nacional de San Luis. Grupo de Estudios Ambientales-IMASL. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nosetto, Marcelo D. Universidad Nacional de San Luis. Grupo de Estudios Ambientales-IMASL. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nosetto, Marcelo D. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias. Cátedra de Climatología; ArgentinaFil: Fernandez, Pedro David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Fernandez, Pedro David. Universidad Nacional de Tucumán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ecología Regional (IER); ArgentinaFil: Castellanos, George. Universidad Nacional de San Luis. Grupo de Estudios Ambientales-IMASL. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Magliano, Patricio N. Universidad Nacional de San Luis. Grupo de Estudios Ambientales-IMASL. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Magliano, Patricio N. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Biología; Argentin

Effects of simulated altitude (normobaric hypoxia) on cardiorespiratory parameters and circulating endothelial precursors in healthy subjects

<p>Abstract</p> <p>Background</p> <p>Circulating Endothelial Precursors (PB-EPCs) are involved in the maintenance of the endothelial compartment being promptly mobilized after injuries of the vascular endothelium, but the effects of a brief normobaric hypoxia on PB-EPCs in healthy subjects are scarcely studied.</p> <p>Methods</p> <p>Clinical and molecular parameters were investigated in healthy subjects (n = 8) in basal conditions (T0) and after 1 h of normobaric hypoxia (T1), with Inspiratory Fraction of Oxygen set at 11.2% simulating 4850 mt of altitude. Blood samples were obtained at T0 and T1, as well as 7 days after hypoxia (T2).</p> <p>Results</p> <p>In all studied subjects we observed a prompt and significant increase in PB-EPCs, with a return to basal value at T2. The induction of hypoxia was confirmed by Alveolar Oxygen Partial Pressure (PAO₂) and Spot Oxygen Saturation decreases. Heart rate increased, but arterial pressure and respiratory response were unaffected. The change in PB-EPCs percent from T0 to T1 was inversely related to PAO₂at T1. Rapid (T1) increases in serum levels of hepatocyte growth factor and erythropoietin, as well as in cellular PB-EPCs-expression of Hypoxia Inducible Factor-1α were observed.</p> <p>Conclusion</p> <p>In conclusion, the endothelial compartment seems quite responsive to standardized brief hypoxia, possibly important for PB-EPCs activation and recruitment.</p

Release of soluble vascular endothelial growth factor receptor-1 (sFlt-1) during coronary artery bypass surgery

<p>Abstract</p> <p>Background</p> <p>This study was conducted to follow plasma concentrations of sFlt-1 and sKDR, two soluble forms of the vascular endothelial growth factor (VEGF) receptor in patients undergoing coronary artery bypass graft (CABG) surgery with extracorporeal circulation (ECC).</p> <p>Methods</p> <p>Plasma samples were obtained before, during and after surgery in 15 patients scheduled to undergo CABG. Levels of sFlt-1 and KDR levels were investigated using specific ELISA.</p> <p>Results</p> <p>A 75-fold increase of sFlt-1 was found during cardiac surgery, sFlt-1 levels returning to pre-operative values at the 6^thpost-operative hour. In contrast sKDR levels did not change during surgery. The ECC-derived sFlt-1 was functional as judge by its inhibitory effect on the VEGF mitogenic response in human umbilical vein endothelial cells (HUVECs). Kinetic experiments revealed sFlt-1 release immediately after the beginning of ECC suggesting a proteolysis of its membrane form (mFlt-1) rather than an elevated transcription/translation process. Flow cytometry analysis highlighted no effect of ECC on the shedding of mFlt-1 on platelets and leukocytes suggesting vascular endothelial cell as a putative cell source for the ECC-derived sFlt-1.</p> <p>Conclusion</p> <p>sFlt-1 is released during CABG with ECC. It might be suggested that sFlt-1 production, by neutralizing VEGF and/or by inactivating membrane-bound Flt-1 and KDR receptors, might play a role in the occurrence of post-CABG complication.</p

Cut-off point for WHOQOL-bref as a measure of quality of life of older adults

OBJECTIVE To propose a cut-off for the World Health Organization Quality of Life-Bref (WHOQOL-bref) as a predictor of quality of life in older adults.METHODS Cross-sectional study with 391 older adults registered in the Northwest Health District in Belo Horizonte, MG, Southeastern Brazil, between October 8, 2010 and May 23, 2011. The older adults’ quality of life was measured using the WHOQOL-bref. The analysis was rationalized by outlining two extreme and simultaneous groups according to perceived quality of life and satisfaction with health (quality of life good/satisfactory – good or very good self-reported quality of life and being satisfied or very satisfied with health – G5; and poor/very poor quality of life – poor or very poor self-reported quality of life and feeling dissatisfied or very dissatisfied with health – G6). A Receiver-Operating Characteristic curve (ROC) was created to assess the diagnostic ability of different cut-off points of the WHOQOL-bref.RESULTS ROC curve analysis indicated a critical value 60 as the optimal cut-off point for assessing perceived quality of life and satisfaction with health. The area under the curve was 0.758, with a sensitivity of 76.8% and specificity of 63.8% for a cut-off of ≥ 60 for overall quality of life (G5) and sensitivity 95.0% and specificity of 54.4% for a cut-off of < 60 for overall quality of life (G6).CONCLUSIONS Diagnostic interpretation of the ROC curve revealed that cut-off < 60 for overall quality of life obtained excellent sensitivity and negative predictive value for tracking older adults with probable worse quality of life and dissatisfied with health

Renal Thrombotic Microangiopathy in Mice with Combined Deletion of Endocytic Recycling Regulators EHD3 and EHD4

Eps15 Homology Domain-containing 3 (EHD3), a member of the EHD protein family that regulates endocytic recycling, is the first protein reported to be specifically expressed in the glomerular endothelium in the kidney; therefore we generated Ehd3–/– mice and assessed renal development and pathology. Ehd3–/– animals showed no overt defects, and exhibited no proteinuria or glomerular pathology. However, as the expression of EHD4, a related family member, was elevated in the glomerular endothelium of Ehd3–/– mice and suggested functional compensation, we generated and analyzed Ehd3–/–; Ehd4–/– mice. These mice were smaller, possessed smaller and paler kidneys, were proteinuric and died between 3–24 weeks of age. Detailed analyses of Ehd3–/–; Ehd4–/– kidneys demonstrated thrombotic microangiopathy (TMA)-like glomerular lesions including thickening and duplication of glomerular basement membrane, endothelial swelling and loss of fenestrations. Other changes included segmental podocyte foot process effacement, mesangial interposition, and abnormal podocytic and mesangial marker expression. The glomerular lesions observed were strikingly similar to those seen in human pre-eclampsia and mouse models of reduced VEGF expression. As altered glomerular endothelial VEGFR2 expression and localization and increased apoptosis was observed in the absence of EHD3 and EHD4, we propose that EHD-mediated endocytic traffic of key surface receptors such as VEGFR2 is essential for physiological control of glomerular function. Furthermore, Ehd3–/–; Ehd4–/– mice provide a unique model to elucidate mechanisms of glomerular endothelial injury which is observed in a wide variety of human renal and extra-renal diseases

Comparison of the ligand‐binding properties of fluorescent VEGF‐A isoforms to VEGF receptor 2 in living cells and membrane preparations using NanoBRET

Background and Purpose: Vascular Endothelial Growth Factor A (VEGF-A) is a key mediator of angiogenesis. A striking feature of the binding of a fluorescent analogue of VEGF165a to NanoLuciferase-tagged VEGF Receptor 2 (VEGFR2) in living cells is that the bioluminescence resonance energy transfer (BRET) signal is not sustained and declines over time. This may be secondary to receptor internalisation. Here we have compared the binding of three fluorescent VEGF-A isoforms to VEGFR2 in cells and isolated membrane preparations.Experimental Approach: Ligand binding kinetics were monitored in both intact HEK293T cells and membranes (expressing NanoLuciferase tagged VEGFR2) using BRET between the tagged receptor and fluorescent analogues of VEGF165a, VEGF165b and VEGF121a. VEGFR2 endocytosis in intact cells expressing VEGFR2 was monitored by following the appearance of fluorescent ligand-associated receptors in intracellular endosomes using automated quantitative imaging.Key Results: Quantitiative analysis of the effect of fluorescent VEGF-A isoforms onVEGFR2 endocytosis in cells demonstrated that they produced a rapid and potent translocation of ligand-bound VEGFR2 into intracellular endosomes. NanoBRET can be used to monitor the kinetics of the binding of fluorescent VEGF-A isoforms to VEGFR2. In isolated membrane preparations, ligand binding association curves were maintained for the duration of the 90 minute experiment. Measurement of koff at pH 6.0 in membrane preparations indicated shorter ligand residence times than those obtained at pH 7.4.Conclusions and Implications: These studies suggest that rapid VEGF-A isoform-induced receptor endocytosis shortens agonist residence times on the receptor (1/koff) as VEGFR2 moves from the plasma membrane to intracellular endosomes