Determinação de elementos traço em carvão por espectrometria de absorção atômica de alta resolução com fonte contínua e por espectrometria de massa com plasma indutivamente acoplado e vaporização eletrotérmica

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-Graduação em Química.Neste trabalho, o desenvolvimento de método para determinação de Cd e Pb por espectrometria de absorção atômica de alta resolução com fonte contínua com introdução direta da amostra sólida (SS-HR-CS AAS) e para determinação de As, Cd, Pb e Tl por espectrometria de massa com plasma indutivamente acoplado e vaporização eletrotérmica (ETV-ICP-MS) é demonstrado para a análise de amostras certificadas de referência de carvão. Para a amostragem direta de sólidos com análise por HR-CS AAS, as amostram foram moídas e inseridas em alíquotas de massa de 0,6 a 1 mg. Temperaturas de pirólise inferiores a 600 ºC geram fundo contínuo devido à eliminação incompleta da matriz e temperaturas elevadas de atomização propiciam o aparecimento de fundo estruturado. Após calibração contra padrões aquosos em HNO3 0,5% v/v, a determinação foi realizada com uso de Ir como modificador permanente para Cd e sem uso de modificador para Pb, resultando em boa concordância entre valores de referência e valores determinados. Para análise por ETV-ICP-MS, as suspensões (5 mg mL-1) foram preparadas em HNO3 5% v/v. Temperatura de pirólise e vaporização e a vazão dos gases interno e carreador foram otimizados. Ru foi adicionado em solução, agindo como modificador e como carreador do aerossol. Após calibração externa com padrões aquosos, bons resultados foram obtidos na análise dos materiais de referência

Análise direta de sólidos por espectrometria de absorção atômica de alta resolução com fonte contínua e investigação de compostos organometálicos por análise direta em tempo real com espectrometria de massa

Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-Graduação em Química.O presente trabalho, em sua primeira parte, descreve o desenvolvimento de métodos analíticos para a determinação de elementos traço e consituintes minoritários por espectrometria de absorção atômica de alta resolução com fonte contínua (HR-CS AAS), com análise direta de amostras sólidas (SS). Inicialmente, são mostrado resultados para a determinação de Pb em amostras biológicas. O procedimento utilizou Ru como modificador permanente e calibração utilizando padrões aquosos, com bons resultados para a análise de sete materiais de referência certificados. A correção de fundo por mínimos quadrados (LSBC) demonstrou que o espectro molecular observado em temperaturas elevadas de atomização correspondia a monóxido de fósforo (PO). A determinação de Ag, por sua vez, foi conduzida em amostras geológicas, sem uso de modificadores. Para amostras de solos e sedimentos, calibração com padrões aquosos permitiu a obtenção de resultados concordantes com valores informados, ao passo que a análise de rochas e minérios requereu calibração com material de referência sólido. A LSBC foi conduzida para demonstrar a correção para fundo estruturado decorrente da molécula de SH. Em uma terceira aplicação, Cr e Fe foram simultaneamente determinados em amostras de alimentos por SS-HR-CS AAS, através da linha principal de Cr em 357,869 nm e de uma linha secundária de Fe em 358,119 nm. Bons resultados foram obtidos para a análise de 4 materiais de referência certificados, comprovando a boa exatidão para a análise simultânea. Por fim, nanotubos de carbono de parede simples e de paredes múltiplas foram analisados para a determinação de Ag, Co, Cr, Ni e Pb por SS-HR-CS AAS. A determinação de Ag e Pb procedeu de maneira análoga aos procedimentos anteriormente descritos, embora sem uso de modificador, ao passo que Co, Cr e Ni foram determinados de maneira simultânea, na região espectral em torno da linha secundária de Cr em 360,538 nm. Em função das concentrações elevadas, as asas das linhas foram utilizadas para propósitos analíticos, resultando em redução de sensibilidade e aumento de linearidade das curvas de calibração. Boa concordância foi obtida na comparação com resultados obtidos por procedimento de extração e análise por espectrometria de massa com plasma indutivamente acoplado (ICP-MS). Em todos os procedimentos, limites de detecção variando entre 0,002 µg g-1 (Ag) e 1,5 mg g-1 (Co em linha secundária) foram obtidos, sendo adequados aos métodos propostos. A segunda parte do trabalho envolveu a investigação de compostos organometálicos utilizando a espectrometria de massa (MS) com análise direta em tempo real (DART). Diversos compostos organometálicos de As, Fe, Hg, Pb, Se e Sn foram analisados por MS quanto aos sinais correspondentes obtidos por ionização pela fonte DART. Padrões de fragmentação colisional induzida foram também avaliados. Dois procedimentos distintos de amostragem, com algodão exposto ao plasma da fonte DART e com análise do headspace dos compostos puros e/ou dissolvidos em tolueno, foram utilizados. A amostragem por headspace permitiu obter sinais temporalmente estáveis, possibilitando avaliar os efeitos dos parâmetros operacionais da fonte DART sobre os sinais obtidos. Constatou-se que a ativação de dois dos eletrodos que compõem a fonte DART, com a função eliminar íons do fluxo de gás, causou diminuição na intensidade dos sinais para a maioria dos compostos, o que pode ser indício da participação de espécies iônicas do plasma na ionização dos diferentes compostos. Algumas tentativas de aplicação dos procedimentos descritos à detecção de organometálicos em amostras "reais" foram conduzidas, embora apenas sinais de baixa intensidade em razões m/z elevadas tenham sido detectados. The present work describes, in its first section, the development of analytical methods aiming to determine trace elements and minor constituents using high-resolution continuum source atomic absorption spectrometry (HR-CS AAS) and direct solid sampling (SS). Initially, results are shown for the determination of Pb in biological samples. The procedure employed Ru as permanent modifier and calibration against aqueous standards, resulting in good agreement between determined and certified values for the analysis of seven certified reference materials. Least-squares background correction is shown to demonstrate that the molecular spectrum observed at elevated atomization temperatures is due to the presence of the phosphorus monoxide (PO) molecule. The determination of Ag has also been carried out, without using chemical modifiers, in geological samples. For soil and sediment analysis, calibration against aqueous standards allowed good agreement between informed and determined values, whereas rock and ore analysis required calibration against a solid reference material. LSBC was employed in order to demonstrate that the molecular spectrum detected was due to the SH molecule. In a third independent procedure, Cr and Fe have been simultaneously determined in food samples using the main Cr line at 357.869 nm and a secondary Fe line at 358.119 nm. Good results were obtained for the analysis of four certified reference materials, attesting the good accuracy of the simultaneous determination procedure. Finally, single and multi-walled carbon nanotubes have been analyzed, aiming to determine Ag, Co, Cr, Ni and Pb by SS-HR-CS AAS. The determination of Ag and Pb was carried out similarly to the procedures previously described, except for the absence of chemical modifiers for Pb. Cobalt, Cr and Ni were determined simultaneously, in the spectral region in the vicinity of the secondary Cr line at 360.538 nm. Due to the high concentration found in the carbon nanotubes samples, line wings have been used for analytical purposes, resulting in reduced sensitivity and increased linear working range. Good agreement with the concentration of Co, Ni and Pb was obtained in comparison to the results determined by a microwave-assisted extraction procedure and inductively coupled plasma mass spectrometry (ICP-MS). All the developed methodologies resulted in detection limits ranging between 0.002 µg g-1 (Ag) and 1.5 mg g-1 (secondary Co line), which have proved to be adequate to the proposed analytical procedures. The second part of the work describes the investigation that has been carried out using organometallic compounds and mass spectrometry (MS) with direct analysis in real time (DART). Several organometallic compounds of As, Fe, Hg, Pb, Se and Sn have been used in the investigation, aiming to identify their corresponding mass spectra obtained using DART as the ion source. Induced fragmentation patterns have also been evaluated. Two distinct sampling procedures, using cotton exposed to the DART plasma or headspace sampling of pure or toluene-solutions of the organometallic compounds, have been employed. Headspace sampling allowed temporally stable signals to be obtained, allowing to evaluate the effect of DART operating parameters on the intensity and pattern of the signals obtained. Results have shown that the activation of two of the electrodes that integrate the DART source, which are supposed to remove ions from the gas stream, resulted in the reduction in signal intensity for most of the compounds investigated, which seems to indicate the ionic species of the plasma participate in the ionization process of the organometallic compounds. A few attempts to identify organometallic signals in 'real' samples have been carried out, although only low-intensity signals at high m/z ratios could be observed

Estudo de adesão e proliferação celular sobre superfícies de filmes poliméricos modificados por processo de plasma frio com descarga de barreira dielétrica

Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-Graduação em Química.Este trabalho descreve a síntese de filmes poliméricos, sua modificação e a avaliação quanto à adesão de espécies celulares. Filmes de poliestireno (PS) e poli (metacrilato de metila) (PMMA) e mistura com 1:1 de composição preparados por casting foram avaliados quanto ao crescimento e proliferação de fibroblastos L-929. Estudos envolvendo modificação da superfície destes polímeros foram desenvolvidos, com resultados promissores decorrentes da utilização de plasma frio através de descarga de barreira dielétrica (DBD) para induzir a modificação da superfície, após planejamento fatorial das condições experimentais. Alterações nas propriedades da superfície com relação às amostras não tratadas foram acompanhadas por medidas de ângulo de contato, energia de superfície, histerese, microscopia eletrônica de varredura (SEM) e SEM com espectrometria de energia dispersiva, microscopia de força atômica (AFM) e espectrometria fotoeletrônica de raios-X. Após o tratamento, excelente aderência e proliferação de células foram observadas em todos os filmes com maior proliferação celular no filme PS/PMMA 1:1. Filmes finos de zeína obtidos por spin coating foram também tratados por plasma frio com DBD. Após o tratamento, uma pequena variação de molhabilidade foi detectada, além de aumento de rugosidade. A adsorção de albumina de soro bovino na superfície dos filmes foi também avaliada. Todos os filmes apresentaram excelente adesão das células L-929 após os dois tratamentos. Resultados preliminares para filmes finos nanoestruturados formados a partir de dois copolímeros em bloco de poliestireno e poli(ácido acrílico) são também descritos. Ambos os filmes, após análise por AFM, apresentaram superfícies mais rugosas e uma significativa melhora na adesão e proliferação das células L-929, o que os torna potenciais biomateriais para aplicação biomédica.This work describes the synthesis of polymeric films, their surface modification and an evaluation of their ability to promote adhesion of cell species. Films formed with the casting technique from poly(styrene) (PS), poly (methyl methacrylate) (PMMA) and a 1:1 mixture of both were evaluated towards the growth and proliferation of L-929 fibroblasts. Studies involving the surface modification of the polymeric films were carried out, with promising results obtained from the use of cold plasma generated by a dielectric barrier discharge (DBD), following a factorial design for optimization of the operating parameters. Changes in the surface properties of the plasma-treated films were evaluated by means of contact angle, surface energy and hysteresis measurements, scanning electron microscopy (SEM) and energy-dispersive SEM, atomic force microscopy (AFM) and X-ray photoelectronic spectrometry. Substantial proliferation of cells was observed on the surface of all plasma-treated films, with a superior performance of the PS/PMMA 1:1 film. Thin films produced from zein using the spin coating technique were also submitted to DBD-cold plasma treatment. A minor variation in the wetability of the plasma-treated films was observed, in addition to increased rugosity. The effect of adsorption of bovine serum albumin on the polymeric film surface was also evaluated. Both surface-based treatments resulted in enhanced adhesion of L-929 cells on the polymeric films. Preliminary results for nanostructured thin films formed from two block copolymers of poly(styrene) and poly(acrylic acid) are also presented. AFM analysis of the surface of both plasma-treated films evidenced increased rugosity, which was accompanied by a substantial improvement in the adhesion and proliferation of L-929 cells, turning these polymeric films into promising biomaterials

Teaching of the Integrated Management of Childhood Illness strategy in undergraduate nursing programs

Objective: To describe and analyze the teaching of the Integrated Management of hildhood Illness (IMCI) strategy on Brazilian undergraduate nursing programs. Method: Integrating an international multicentric study, a cross-sectional online survey was conducted between May and October 2010 with 571 undergraduate nursing programs in Brazil Results: Responses were received from 142 programs, 75% private and 25% public. 64% of them included the IMCI strategy in the theoretical content, and 50% of the programs included IMCI as part of the students’ practical experience. The locations most used for practical teaching were primary health care units. The ‘treatment’ module was taught by the fewest number of programs, and few programs had access to the IMCI instructional manuals. All programs used exams for evaluation, and private institutions were more likely to include class participation as part of the evaluation. Teaching staff in public institutions were more likely to have received training in teaching IMCI. Conclusion: In spite of the relevance of the IMCI strategy in care of the child, its content is not addressed in all undergraduate programs in Brazil, and many programs do not have access to the IMCI teaching manuals and have not provide training in IMCI to their teaching staff

Ensino da estratégia Atenção Integrada às Doenças Prevalentes na Infância na graduação em enfermagem

OBJECTIVE: To describe and analyze the teaching of the Integrated Management of Childhood Illness (IMCI) strategy on Brazilian undergraduate nursing programs. METHOD: Integrating an international multicentric study, a cross-sectional online survey was conducted between May and October 2010 with 571 undergraduate nursing programs in Brazil RESULTS: Responses were received from 142 programs, 75% private and 25% public. 64% of them included the IMCI strategy in the theoretical content, and 50% of the programs included IMCI as part of the students' practical experience. The locations most used for practical teaching were primary health care units. The 'treatment' module was taught by the fewest number of programs, and few programs had access to the IMCI instructional manuals. All programs used exams for evaluation, and private institutions were more likely to include class participation as part of the evaluation. Teaching staff in public institutions were more likely to have received training in teaching IMCI. CONCLUSION: In spite of the relevance of the IMCI strategy in care of the child, its content is not addressed in all undergraduate programs in Brazil, and many programs do not have access to the IMCI teaching manuals and have not provide training in IMCI to their teaching staff.OBJETIVO: se describió y analizó la enseñanza de la estrategia Atención Integrada a las Enfermedades Prevalentes de la Infancia en la formación de licenciados en enfermería en Brasil. MÉTODO: estudio transversal que integró una investigación internacional multicéntrica. Fueron invitados a acceder al cuestionario electrónico, 571 cursos identificados en el país. La recolección de datos fue realizada de mayo a octubre de 2010. RESULTADOS: 142 cursos respondieron al cuestionario, siendo tres cuartos privados. La estrategia Atención Integrada a las Enfermedades Prevalentes de la Infancia integraba el contenido teórico de 64% y práctico de 50% de los cursos. La práctica ocurría principalmente en servicios de atención primaria. El módulo tratamiento era el menos enseñado y pocos cursos poseían los manuales de la estrategia. Todos evaluaban los estudiantes con pruebas; y en las privadas si valoraba además la participación en clase. Las instituciones públicas tenían más docentes capacitados en la estrategia. CONCLUSÍON: a pesar de su relevancia en el cuidado al niño, la estrategia Atención Integrada a las Enfermedades Prevalentes de la Infancia no está incorporada en todos los cursos de licenciatura en enfermería en Brasil y falta capacitación docente y material didáctico para hacerlo.OBJETIVO: descrever e analisar o ensino da Estratégia Atenção Integrada às Doenças Prevalentes na Infância, praticado na graduação em enfermagem no Brasil. MÉTODO: estudo transversal que integrou pesquisa internacional. Foram convidados a acessar o questionário eletrônico 571 cursos de graduação identificados no país. A coleta de dados foi realizada de maio a outubro de 2010. RESULTADOS: responderam ao questionário 142 cursos, sendo 3/4 (três quartos) privados, com algumas diferenças do ensino entre as instituições públicas e privadas. A estratégia Atenção Integrada às Doenças Prevalentes na Infância integrava o conteúdo teórico de 64% dos cursos e o conteúdo prático de 50%. Unidades básicas de saúde eram os locais mais usados para ensino prático. O módulo tratamento era o menos ensinado e poucos cursos possuíam os manuais da estratégia. Todos utilizavam provas para avaliação, mas a participação em aulas era mais considerada nas privadas. Houve diferença somente quanto à capacitação docente na estratégia, com maioria nas instituições públicas. CONCLUSÃO: apesar da relevância da estratégia Atenção Integrada às Doenças Prevalentes na Infância no cuidado à criança, seu conteúdo não é abordado em todos os cursos de graduação e constatam-se falta de capacitação docente e de material didático

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Background: Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. // Methods: We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung's disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. // Findings: We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung's disease) from 264 hospitals (89 in high-income countries, 166 in middle-income countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in low-income countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. // Interpretation: Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between low-income, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Teaching of the Integrated Management of Childhood Illness strategy in undergraduate nursing programs Ense&#241;anza de la estrategia Atenci&#243;n Integrada a las Enfermedades Prevalentes de la Infancia en enfermer&#237;a Ensino da estrat&#233;gia Aten&#231;&#227;o Integrada &#224;s Doen&#231;as Prevalentes na Inf&#226;ncia na gradua&#231;&#227;o em enfermagem

OBJECTIVE: To describe and analyze the teaching of the Integrated Management of Childhood Illness (IMCI) strategy on Brazilian undergraduate nursing programs. METHOD: Integrating an international multicentric study, a cross-sectional online survey was conducted between May and October 2010 with 571 undergraduate nursing programs in Brazil RESULTS: Responses were received from 142 programs, 75% private and 25% public. 64% of them included the IMCI strategy in the theoretical content, and 50% of the programs included IMCI as part of the students' practical experience. The locations most used for practical teaching were primary health care units. The 'treatment' module was taught by the fewest number of programs, and few programs had access to the IMCI instructional manuals. All programs used exams for evaluation, and private institutions were more likely to include class participation as part of the evaluation. Teaching staff in public institutions were more likely to have received training in teaching IMCI. CONCLUSION: In spite of the relevance of the IMCI strategy in care of the child, its content is not addressed in all undergraduate programs in Brazil, and many programs do not have access to the IMCI teaching manuals and have not provide training in IMCI to their teaching staff.<br> OBJETIVO: se describi&#243; y analiz&#243; la ense&#241;anza de la estrategia Atenci&#243;n Integrada a las Enfermedades Prevalentes de la Infancia en la formaci&#243;n de licenciados en enfermer&#237;a en Brasil. M&#201;TODO: estudio transversal que integr&#243; una investigaci&#243;n internacional multic&#233;ntrica. Fueron invitados a acceder al cuestionario electr&#243;nico, 571 cursos identificados en el pa&#237;s. La recolecci&#243;n de datos fue realizada de mayo a octubre de 2010. RESULTADOS: 142 cursos respondieron al cuestionario, siendo tres cuartos privados. La estrategia Atenci&#243;n Integrada a las Enfermedades Prevalentes de la Infancia integraba el contenido te&#243;rico de 64% y pr&#225;ctico de 50% de los cursos. La pr&#225;ctica ocurr&#237;a principalmente en servicios de atenci&#243;n primaria. El m&#243;dulo tratamiento era el menos ense&#241;ado y pocos cursos pose&#237;an los manuales de la estrategia. Todos evaluaban los estudiantes con pruebas; y en las privadas si valoraba adem&#225;s la participaci&#243;n en clase. Las instituciones p&#250;blicas ten&#237;an m&#225;s docentes capacitados en la estrategia. CONCLUS&#205;ON: a pesar de su relevancia en el cuidado al ni&#241;o, la estrategia Atenci&#243;n Integrada a las Enfermedades Prevalentes de la Infancia no est&#225; incorporada en todos los cursos de licenciatura en enfermer&#237;a en Brasil y falta capacitaci&#243;n docente y material did&#225;ctico para hacerlo.<br> OBJETIVO: descrever e analisar o ensino da Estrat&#233;gia Aten&#231;&#227;o Integrada &#224;s Doen&#231;as Prevalentes na Inf&#226;ncia, praticado na gradua&#231;&#227;o em enfermagem no Brasil. M&#201;TODO: estudo transversal que integrou pesquisa internacional. Foram convidados a acessar o question&#225;rio eletr&#244;nico 571 cursos de gradua&#231;&#227;o identificados no pa&#237;s. A coleta de dados foi realizada de maio a outubro de 2010. RESULTADOS: responderam ao question&#225;rio 142 cursos, sendo 3/4 (tr&#234;s quartos) privados, com algumas diferen&#231;as do ensino entre as institui&#231;&#245;es p&#250;blicas e privadas. A estrat&#233;gia Aten&#231;&#227;o Integrada &#224;s Doen&#231;as Prevalentes na Inf&#226;ncia integrava o conte&#250;do te&#243;rico de 64% dos cursos e o conte&#250;do pr&#225;tico de 50%. Unidades b&#225;sicas de sa&#250;de eram os locais mais usados para ensino pr&#225;tico. O m&#243;dulo tratamento era o menos ensinado e poucos cursos possu&#237;am os manuais da estrat&#233;gia. Todos utilizavam provas para avalia&#231;&#227;o, mas a participa&#231;&#227;o em aulas era mais considerada nas privadas. Houve diferen&#231;a somente quanto &#224; capacita&#231;&#227;o docente na estrat&#233;gia, com maioria nas institui&#231;&#245;es p&#250;blicas. CONCLUS&#195;O: apesar da relev&#226;ncia da estrat&#233;gia Aten&#231;&#227;o Integrada &#224;s Doen&#231;as Prevalentes na Inf&#226;ncia no cuidado &#224; crian&#231;a, seu conte&#250;do n&#227;o &#233; abordado em todos os cursos de gradua&#231;&#227;o e constatam-se falta de capacita&#231;&#227;o docente e de material did&#225;tico