Predicting the prices of cards in the game Magic with machine learning

V tem diplomskem delu smo preučevali trende gibanja cen pri kartah igre Magic: The Gathering in pri tem uporabili najbolj primerne metode strojnega ućenja. Cilj je bil izdelati napovedni model za cene kart. Naša naloga je bila identiciranje pomembnih virov, pridobivanje potrebnih podatkov, njihova pretvorba v računalniku razumljivo obliko ter izbira primernega algoritma. Model, ki smo ga ustvarili, se je izkazal za zanesljivega s 61% točnostjo napovedi gibanja cene pri zelo redkih kartah, medtem ko smo pri redkih kartah dosegli le 52% točnost, kar ni preseglo niti privzete točnosti. Pri nalogi smo uporabili metodo podpornih vektorjev ter si pomagali z orodjem Weka. S podatki, ki smo jih pridobili, smo naredili še nekaj poizkusov in tako poiskali nekaj novih odvisnosti med podatki, ki jih prej nismo poznali.This thesis is a study of Magic: The Gathering card price fluctuations using the most appropriate machine learning methods. The goal was to construct a predictive model for card prices. This required us to identify crucial attributes, gather necessary data, convert it to a machine-readable format and select a suitable learning algorithm for the task. The resulting model was effective, attaining a 61 % price trend accuracy with mythic rare cards, while it was less successful with rare cards with only 52% accuracy, which failed to beat default accuracy. Support vector machines algorithms and the machine learning toolbox Weka were used to achieve these results, which were applied in further experiments that led to the discovery of previously unknown data dependencies

Use of a trabecular metal implant in ankle arthrodesis after failed total ankle replacement: A short-term follow-up of 13 patients

Patients and methods 13 patients with a migrated or loose total ankle implant underwent arthrodesis with the use of a retrograde intramedullary nail through a trabecular metal Tibial Cone. The mean follow-up time was 1.4 (0.6-3.4) years. Results At the last examination, 7 patients were pain-free, while 5 had some residual pain but were satisfied with the procedure. 1 patient was dissatisfied and experienced pain and swelling when walking. The implant-bone interfaces showed no radiographic zones or gaps in any patient, indicating union. Interpretation The method is a new way of simplifying and overcoming some of the problems of performing arthrodesis after failed total ankle replacement

Severe hematopoietic stem cell inflammation compromises chronic granulomatous disease gene therapy

X-linked chronic granulomatous disease (CGD) is associated with defective phagocytosis, life-threatening infections, and inflammatory complications. We performed a clinical trial of lentivirus-based gene therapy in four patients (NCT02757911). Two patients show stable engraftment and clinical benefits, whereas the other two have progressively lost gene-corrected cells. Single-cell transcriptomic analysis reveals a significantly lower frequency of hematopoietic stem cells (HSCs) in CGD patients, especially in the two patients with defective engraftment. These two present a profound change in HSC status, a high interferon score, and elevated myeloid progenitor frequency. We use elastic-net logistic regression to identify a set of 51 interferon genes and transcription factors that predict the failure of HSC engraftment. In one patient, an aberrant HSC state with elevated CEBPβ expression drives HSC exhaustion, as demonstrated by low repopulation in a xenotransplantation model. Targeted treatments to protect HSCs, coupled to targeted gene expression screening, might improve clinical outcomes in CGD

Simulated Microgravity Compromises Mouse Oocyte Maturation by Disrupting Meiotic Spindle Organization and Inducing Cytoplasmic Blebbing

In the present study, we discovered that mouse oocyte maturation was inhibited by simulated microgravity via disturbing spindle organization. We cultured mouse oocytes under microgravity condition simulated by NASA's rotary cell culture system, examined the maturation rate and observed the spindle morphology (organization of cytoskeleton) during the mouse oocytes meiotic maturation. While the rate of germinal vesicle breakdown did not differ between 1 g gravity and simulated microgravity, rate of oocyte maturation decreased significantly in simulated microgravity. The rate of maturation was 8.94% in simulated microgravity and was 73.0% in 1 g gravity. The results show that the maturation of mouse oocytes in vitro was inhibited by the simulated microgravity. The spindle morphology observation shows that the microtubules and chromosomes can not form a complete spindle during oocyte meiotic maturation under simulated microgravity. And the disorder of γ-tubulin may partially result in disorganization of microtubules under simulated microgravity. These observations suggest that the meiotic spindle organization is gravity dependent. Although the spindle organization was disrupted by simulated microgravity, the function and organization of microfilaments were not pronouncedly affected by simulated microgravity. And we found that simulated microgravity induced oocytes cytoplasmic blebbing via an unknown mechanism. Transmission electron microscope detection showed that the components of the blebs were identified with the cytoplasm. Collectively, these results indicated that the simulated microgravity inhibits mouse oocyte maturation via disturbing spindle organization and inducing cytoplasmic blebbing

MCT Expression and Lactate Influx/Efflux in Tanycytes Involved in Glia-Neuron Metabolic Interaction

Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes

Présence en France de deux parasites d'origine est-asiatique : Diplozoon nipponicum Goto, 1891 (Monogenea) et Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda) chez Cyprinus carpio (Teleostei, Cyprinidae)

Nous signalons pour la première fois en France la présence de Diplozoon nipponicum Goto, 1891 (Plathelminthe, Monogenea) et de Bothriocephalus acheilognathi Yamaguti, 1934 (Plathelminthe, Cestoda) tous deux récoltés chez Cyprinus carpio en élevage semi-intensif en Camargue. Ces deux parasites sont d'origine est-asiatique. Après avoir rappelé leur importance relative en pathologie de poissons, nous discutons des modalités de leur introduction en France