First evidence for postzygotic reproductive isolation between two populations of Eurasian perch (Perca fluviatilis L.) within Lake Constance

<p>Abstract</p> <p>Background</p> <p>The evolution of reproductive traits, such as hybrid incompatibility (postzygotic isolation) and species recognition (prezygotic isolation), have shown their key role in speciation. Theoretical modeling has recently predicted that close linkage between genes controlling pre- and postzygotic reproductive isolation could accelerate the conditions for speciation. Postzygotic isolation could develop during the sympatric speciation process contributing to the divergence of populations. Using hybrid fitness as a measure of postzygotic reproductive isolation, we empirically studied population divergence in perch (<it>Perca fluviatilis </it>L.) from two genetically divergent populations within a lake.</p> <p>Results</p> <p>During spawning time of perch we artificially created parental offspring and F₁hybrids of the two populations and studied fertilization rate and hatching success under laboratory conditions. The combined fitness measure (product of fertilization rate and hatching success) of F₁hybrids was significantly reduced compared to offspring from within population crosses.</p> <p>Conclusion</p> <p>Our results suggest intrinsic genetic incompatibility between the two populations and indicate that population divergence between two populations of perch inhabiting the same lake may indeed be promoted by postzygotic isolation.</p

Kin and population recognition in sympatric Lake Constance perch (Perca fluviatilis L.) : can assortative shoaling drive population divergence?

Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Springer-Verlag GmbH for personal use, not for redistribution. The definitive version was published in Behavioral Ecology and Sociobiology 59 (2006): 461-468, doi:10.1007/s00265-005-0070-3.Prior studies have shown that perch (Perca fluviatilis L.) of Lake Constance belong to two genetically different but sympatric populations, and that local aggregations of juveniles and adults contain closely related kin. In this study we analysed the genetic structure of pelagic perch larvae to investigate, if kin structured shoals already exist during early ontogenetic development or might be the result of homing to natal sites. Analysis of the gene frequencies at five microsatellite loci revealed that 3 out of 5 pelagic aggregations of larvae showed significant accumulation of kin. To investigate possible mechanisms of shoal formation, we tested if perch use olfactory cues to recognize their kin. Choice tests in a fluviarium showed preference for odours of unfamiliar kin versus unfamiliar non-kin. Additionally, we showed that perch could differentiate between the odours of the two sympatric populations and significantly preferred unfamiliar and unrelated conspecifics of their own over the foreign population. Our results present a behavioural mechanism that could lead to the observed formation of kin structured shoals in perch. We further discuss if the ability to discriminate between the own and the foreign population could result in assortative mating within populations and thus form the basis of “socially mediated speciation” in perch.Funding was provided by Deutsche Forschungsgemeinschaft within the collaborative research center SFB 454, Littoral of Lake Constance, the “Fonds der Chemischen Industrie”, University of Konstanz, and the “Konrad Adenauer-Stiftung”

Characterization of EST derived SSRs from the bay scallop, Argopecten irradians

Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Blackwell Publishing for personal use, not for redistribution. The definitive version was published in Molecular Ecology Notes 5 (2005): 567-568, doi:10.1111/j.1471-8286.2005.00996.x.Interest in bay scallop conservation has resulted in organized stock enhancement efforts and increased attention to fisheries management issues. Genetic markers can facilitate the monitoring of enhancement efforts, characterization of wild populations, and optimize hatchery practices. We have identified eight polymorphic simple sequence repeat markers including one dinucleotide, six trinucleotide and one compound dinucleotide repeats, in expressed sequence tags generated from multiple bay scallop cDNA libraries. The numbers of alleles range from two to five. The expected and observed heterozygosities range from 0.093 to 0.720 and 0.095 to 0.600, respectively.This project was supported by National Research Initiative Competitive Grant no. 2003-35206-12834 from the USDA Cooperative State Research, Education, and Extension Service and by a grant from the County of Barnstable Massachusetts, USA (both to SBR)

Characterization of proper optimal elements with variable ordering structures

In vector optimization with a variable ordering structure the partial ordering defined by a convex cone is replaced by a whole family of convex cones, one associated with each element of the space. As these vector optimization problems are not only of interest in applications but also mathematical challenging, in recent publications it was started to develop a comprehensive theory. In doing that also notions of proper efficiency where generalized to variable ordering structures. In this paper we study the relations between several types of proper optimality notions, among others based on local and global approximations of the considered sets. We give scalarization results based on new functionals defined by elements from the dual cones which allow characterizations also in the nonconvex case

On classes of set optimization problems which are reducible to vector optimization problems and its impact on numerical test instances

Set optimization with the set approach has recently gained increasing interest due to its practical relevance. In this problem class one studies optimization problems with a set-valued objective map and defines optimality based on a direct comparison of the images of the objective function, which are sets here. Meanwhile, in the literature a wide range of theoretical tools as scalarization approaches and derivative concepts as well as first numerical algorithms are available. These numerical algorithms require on the one hand test instances where the optimal solution sets are known. On the other hand, in most examples and test instances in the literature only set-valued maps with a very simple structure are used. We study in this paper such special set-valued maps and we show that some of them are such simple that they can equivalently be expressed as a vector optimization problem. Thus we try to start drawing a line between simple set-valued problems and such problems which have no representation as multiobjective problems. Those having a representation can be used for defining test instances for numerical algorithms with easy verifiable optimal solution set

Impact of cyclones on hard coral and metapopulation structure, connectivity and genetic diversity of coral reef fish

Cyclones have one of the greatest effects on the biodiversity of coral reefs and the associated species. But it is unknown how stochastic alterations in habitat structure influence metapopulation structure, connectivity and genetic diversity. From 1993 to 2018, the reefs of the Capricorn Bunker Reef group in the southern part of the Great Barrier Reef were impacted by three tropical cyclones including cyclone Hamish (2009, category 5). This resulted in substantial loss of live habitat-forming coral and coral reef fish communities. Within 6–8 years after cyclones had devastated, live hard corals recovered by 50–60%. We show the relationship between hard coral cover and the abundance of the neon damselfish (Pomacentrus coelestis), the first fish colonizing destroyed reefs. We present the first long-term (2008–2015 years corresponding to 16–24 generations of P. coelestis) population genetic study to understand the impact of cyclones on the meta-population structure, connectivity and genetic diversity of the neon damselfish. After the cyclone, we observed the largest change in the genetic structure at reef populations compared to other years. Simultaneously, allelic richness of genetic microsatellite markers dropped indicating a great loss of genetic diversity, which increased again in subsequent years. Over years, metapopulation dynamics were characterized by high connectivity among fish populations associated with the Capricorn Bunker reefs (2200 km2); however, despite high exchange, genetic patchiness was observed with annual strong genetic divergence between populations among reefs. Some broad similarities in the genetic structure in 2015 could be explained by dispersal from a source reef and the related expansion of local populations. This study has shown that alternating cyclone-driven changes and subsequent recovery phases of coral habitat can greatly influence patterns of reef fish connectivity. The frequency of disturbances determines abundance of fish and genetic diversity within species

Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus

Crustaceans increasingly suffer from the black spot shell disease syndrome, which principally results from bacterial breakdown of their chitinous exoskeleton. Since Cancer pagurus is highly susceptible to this disease, we compared the bacterial communities of black spot affected and non-affected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA. Within each spot, bacterial communities of affected areas were less diverse compared to communities from non-affected areas. Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas. This indicates a reduced and shifted microbial community composition caused by the black spot disease. Different communities found in black spots likely indicate different stages of the disease. In affected areas, Flavobacteriaceae rose up to one of the most abundant and active families, due to massive increase of Aquimarina spp., suggesting a significant role in shell disease syndrome. We isolated 75 bacterial strains from diseased and healthy areas, which primarily affiliated with Proteobacteria and Bacteroidetes, thus reflecting the dominant phyla detected by amplicon sequencing. The ability to degrade chitin was mainly found for Gammaproteobacteria and Aquimarina spp. within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria. Furthermore, one third of the isolates showed antagonistic properties. The combination of bacterial community analysis and the physiological properties of the isolates provides insights into a functional complex epibacterial community on the carapace of C. pagurus. Importance In recent years, the shell disease syndrome was detected for several ecologically and economically important crustacean species. Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas. Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell. By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus we could demonstrate that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases. Furthermore, the community composition dramatically changed, with some taxa rising to high relative abundances and showing increased activity, indicating a strong participation in shell disease. Characterization of bacterial isolates obtained from affected and non-affected spots provided deeper insights in their physiological properties and thus the possible role within the microbiome

Optimality conditions for set optimization using a directional derivative based on generalized Steiner sets

Set-optimization has attracted increasing interest in the last years, as for instance uncertain multiobjective optimization problems lead to such problems with a set- valued objective function. Thereby, from a practical point of view, most of all the so-called set approach is of interest. However, optimality conditions for these problems, for instance using directional derivatives, are still very limited. The key aspect for a useful directional derivative is the definition of a useful set difference for the evaluation of the numerator in the difference quotient. We present here a new set difference which avoids the use of a convex hull and which applies to arbitrary convex sets, and not to strictly convex sets only. The new set difference is based on the new concept of generalized Steiner sets. We introduce the Banach space of generalized Steiner sets as well as an embedding of convex sets in this space using Steiner points. In this Banach space we can easily define a difference and a directional derivative. We use the latter for new optimality conditions for set optimization. Numerical examples illustrate the new concepts

A modification of the [alpha]BB method for box-constrained optimization and an application to inverse kinematics

For many practical applications it is important to determine not only a numerical approximation of one but a representation of the whole set of globally optimal solutions of a non-convex optimization problem. Then one element of this representation may be chosen based on additional information which cannot be formulated as a mathematical function or within a hierarchical problem formulation. We present such an application in the field of robotic design. This application problem can be modeled as a smooth box-constrained optimization problem. For determining a representation of the global optimal solution set with a predefined quality we modify the well known BB method. We illustrate the properties and give a proof for the finiteness and correctness of our modified BB method

Finding Nemo’s clock reveals switch from nocturnal to diurnal activity

Timing mechanisms play a key role in the biology of coral reef fish. Typically, fish larvae leave their reef after hatching, stay for a period in the open ocean before returning to the reef for settlement. During this dispersal, larvae use a time-compensated sun compass for orientation. However, the timing of settlement and how coral reef fish keep track of time via endogenous timing mechanisms is poorly understood. Here, we have studied the behavioural and genetic basis of diel rhythms in the clown anemonefish Amphiprion ocellaris. We document a behavioural shift from nocturnal larvae to diurnal adults, while juveniles show an intermediate pattern of activity which potentially indicates flexibility in the timing of settlement on a host anemone. qRTPCR analysis of six core circadian clock genes (bmal1, clocka, cry1b, per1b, per2, per3) reveals rhythmic gene expression patterns that are comparable in larvae and juveniles, and so do not reflect the corresponding activity changes. By establishing an embryonic cell line, we demonstrate that clown anemonefish possess an endogenous clock with similar properties to that of the zebrafish circadian clock. Furthermore, our study provides a first basis to study the multi-layered interaction of clocks from fish, anemones and their zooxanthellae endosymbionts