Dicer is required for female reproductive tract development and fertility in the mouse.

Dicer encodes a riboendonuclease required for microRNA biosynthesis. Dicer was inactivated in Müllerian duct mesenchyme-derived tissues of the reproductive tract of the mouse, using an Amhr2-Cre allele. Although Amhr2-Cre; Dicer conditional mutant males appeared normal and were fertile, mutant females were infertile. In adult mutant females, there was a reduction in the size of the oviducts and uterine horns. The oviducts were less coiled compared to controls and cysts formed at the isthmus near the uterotubal junction. Unfertilized, degenerate oocytes were commonly found within these cysts, indicating a defect in embryo transit. Beads transferred into the mutant oviduct failed to migrate into the uterus. In addition, blastocysts transferred directly into the mutant uterus did not result in pregnancy. Histological analysis demonstrated that the mutant uterus contained less glandular tissue and often the few glands that remained were found within the myometrium, an abnormal condition known as adenomyosis. In adult mutants, there was ectopic expression of Wnt4 and Wnt5a in the luminal epithelium (LE) and glandular epithelium (GE) of the uterus, and Wnt11 was ectopically expressed in GE. These results demonstrate that Dicer is necessary for postnatal differentiation of Müllerian duct mesenchyme-derived tissues of the female reproductive tract, suggesting that microRNAs are important regulators of female reproductive tract development and fertility

Optimización del Tratamiento Térmico de Productos Tubulares de Acero

[Optimization of Heat Treatment of Steel Tubular Products] El problema a abordar es el de la optimización del tratamiento térmico de productos tubulares de acero, presente en la empresa siderúrgica Tenaris. Más específicamente, se estudia el problema de dar un tratamiento adecuado a cargas retenidas en el horno de revenido. En este caso, el tratamiento térmico se compone de un primer calentamiento de los tubos a temperaturas cercanas a los 900°C, en el que se austeniza el material, seguido por un enfriamiento rápido en el que el material se transforma en martensita. Esta etapa constituye el templado del acero. Para lograr las propiedades metalúrgicas finales especificadas para el producto se realiza un segundo calentamiento a temperaturas entre 500 y 700°C, que se denomina revenido. Para simplificar el análisis, en este trabajo se considera una única propiedad metalúrgica, la dureza, que para el producto que se analiza debe estar en el rango de 270-300 Hv

Molecular and Functional Characterization and Expression Analysis of a Delta6 Fatty Acyl Desaturase cDNA of European Sea Bass (Dicentrarchus labrax L.)

The extent to which fish species can produce highly unsaturated fatty acids (HUFA) from C18 fatty acids varies with their complement of fatty acyl desaturase (FAD) enzymes. Marine fish are unable to produce HUFA at a significant rate due to apparent deficiencies in one or more enzymatic steps in the desaturation/ elongation pathway. It is not known if this is due to a lack of the genes or to tight regulation of the enzymatic activity in some of the transformation steps. In the present study, we report molecular cloning, cDNA, protein and functional analysis of a Δ6 FAD of European sea bass (Dicentrarchus labrax L.), and describe its tissue expression and nutritional regulation. An FAD cDNA contig sequence from brain tissue of sea bass was obtained by gene walking, and full-length cDNA was obtained by amplification using 5′end forward and 3′ end reversed primers. The full length of the sea bass FAD cDNA was 2089 bp, which included a 5′-UTR (untranslated region) of 267 bp, a 3′-UTR of 484 bp and an open reading frame (ORF) of 1338 bp, which specified a protein of 445 amino acids. The mRNA size, estimated by northern blot analysis was 2.1 kb, consistent with the cDNA. Transient expression of Δ6-FAD-EGFP in HeLa cells showed the protein compartmentalized to the endoplasmic reticulum. Functional expression in yeast showed the sea bass cDNA encoded a unifunctional Δ6 FAD enzyme. The sea bass FAD was more active towards 18:3n-3 with 14.5% being converted to 18:4n-3 compared to 5.6% of 18:2n-6 converted to 18:3n-6. Expression of the Δ6 FAD gene in the sea bass tissues showed a rank order of heart, brain, ovary, kidney, adipose tissue and liver as determined by RT-qPCR. Nutritional regulation of gene expression was studied. Diets containing partial substitution of fish oil with rapeseed or linseed oils induced up-regulation of the Δ6 FAD gene; whereas, a diet containing olive oil did not influence the expression. Similarly, when fish oil was partially replaced by blends of vegetable oils, one increased expression and one did not

OpenMutt - 3D Printed Robotic Quadruped

The objective of the OpenMutt project is to build a modular, open-source quadruped as a multidisciplinary research testbed for students and faculty. The design is based on proven models, including the MIT Mini-Cheetah, NYU Open Dynamic Robot, and Bruton’s openDogV3, with modifications to decrease manufacturing time and cost. OpenMutt utilizes 12 brushless motors, each attached to a cycloidal gearbox for actuation. The quarter model has three degrees of freedom, translational and rotational. A remote control will be used for general movement with impedance and PID controllers for torque and joint control. The majority of parts were additively manufactured with Fused Deposition Modeling(FDM) printers using Polylactic Acid(PLA) and Thermoplastic Polyurethane(TPU). A power supply will be used for quarter model testing, while the full model will use an onboard battery with the battery-management system (BMS). Due to the 13:1 gear ratio of the cycloidal gearbox, motors like the ones selected are adaptable to the model. The purpose behind the application of these methods is to ensure a platform that is easy to construct, iterate and learn with

OpenMutt - 3D Printed Robotic Quadruped

Embry-Riddle Aeronautical University is seeking a robotic dog as a research avenue for different biomechanical designs, control systems, and robotic designs for experimentation and study. The quadruped is based on several open-source platforms including James Bruton’s openDogV3, the MIT Mini-Cheetah, and the NYU Open Dynamic Robot Initiative. The implementation of this research will begin with a quarter model, consisting of a singular leg from the hip to the foot. The leg will be mounted on a benchtop test stand that allows for controlled movement and accessible experimentation. The leg will be separate from the full-model quadruped strictly for experimentation and any full-model revisions. The OpenMutt’s quarter model uses 3 Brushless DC Electric Motors (BLDC) attached to 3 cycloidal gearboxes as its main form of actuation. The majority of parts were manufactured using Polylactic Acid (PLA). Some leg testing has already been completed, but a synchronized movement is yet to be completed

Reheating constraints and consistency relations of the Starobinsky model and some of its generalizations

Building on the success of the Starobinsky model in describing the inflationary period of the universe, we investigate two simple generalizations of this model and their constraints imposed by the reheating epoch. The first generalization takes the form $R^{2p}$, while the second is the $\alpha$-Starobinsky model. We first focus on the case where $p=1$ or equivalently, $\alpha=1$, which corresponds to the original Starobinsky model. We derive exact consistency relations between observables and cosmological quantities, without neglecting any terms, and impose the reheating condition $0 < \omega_{re} < 0.25$, where $\omega_{re}$ is the equation of state parameter at the end of reheating. This allows us to obtain new bounds for $n_s$ and $r$ that satisfy this condition and apply them to other observables and cosmological quantities. We repeat this process for the cases where $p \neq 1$ and $\alpha \neq 1$ and find that these generalizations only result in minor modifications of the Starobinsky model, including the potential and the bounds on observables and cosmological quantities.Comment: 17 pages, 2 figure

The SSS phase of RS Ophiuchi observed with Chandra and XMM-Newton I.: Data and preliminary Modeling

The phase of Super-Soft-Source (SSS) emission of the sixth recorded outburst of the recurrent nova RS Oph was observed twice with Chandra and once with XMM-Newton. The observations were taken on days 39.7, 54.0, and 66.9 after outburst. We confirm a 35-sec period on day 54.0 and found that it originates from the SSS emission and not from the shock. We discus the bound-free absorption by neutral elements in the line of sight, resonance absorption lines plus self-absorbed emission line components, collisionally excited emission lines from the shock, He-like intersystem lines, and spectral changes during an episode of high-amplitude variability. We find a decrease of the oxygen K-shell absorption edge that can be explained by photoionization of oxygen. The absorption component has average velocities of -1286+-267 km/s on day 39.7 and of -771+-65 km/s on day 66.9. The wavelengths of the emission line components are consistent with their rest wavelengths as confirmed by measurements of non-self absorbed He-like intersystem lines. We have evidence that these lines originate from the shock rather than the outer layers of the outflow and may be photoexcited in addition to collisional excitations. We found collisionally excited emission lines that are fading at wavelengths shorter than 15A that originate from the radiatively cooling shock. On day 39.5 we find a systematic blue shift of -526+-114 km/s from these lines. We found anomalous He-like f/i ratios which indicates either high densities or significant UV radiation near the plasma where the emission lines are formed. During the phase of strong variability the spectral hardness light curve overlies the total light curve when shifted by 1000sec. This can be explained by photoionization of neutral oxygen in the line of sight if the densities of order 10^{10}-10^{11} cm^{-3}.Comment: 16 pages, 10 figures, 4 tables. Accepted by ApJ; v2: Co-author Woodward adde

Pollen segmentation and feature evaluation for automatic classification in bright-field microscopy

14 págs.; 10 figs.; 7 tabs.; 1 app.© 2014 Elsevier B.V. Besides the well-established healthy properties of pollen, palynology and apiculture are of extreme importance to avoid hard and fast unbalances in our ecosystems. To support such disciplines computer vision comes to alleviate tedious recognition tasks. In this paper we present an applied study of the state of the art in pattern recognition techniques to describe, analyze, and classify pollen grains in an extensive dataset specifically collected (15 types, 120 samples/type). We also propose a novel contour-inner segmentation of grains, improving 50% of accuracy. In addition to published morphological, statistical, and textural descriptors, we introduce a new descriptor to measure the grain's contour profile and a logGabor implementation not tested before for this purpose. We found a significant improvement for certain combinations of descriptors, providing an overall accuracy above 99%. Finally, some palynological features that are still difficult to be integrated in computer systems are discussed.This work has been supported by the European project APIFRESH FP7-SME-2008-2 ‘‘Developing European standards for bee pollen and royal jelly: quality, safety and authenticity’’ and we would like to thank to Mr. Walter Haefeker, President of the European Professional Beekeepers Association (EPBA). J. Victor Marcos is a ‘‘Juan de la Cierva’’ research fellow funded by the Spanish Ministry of Economy and Competitiveness. Rodrigo Nava thanks Consejo Nacional de Ciencia y Tecnología (CONACYT) and PAPIIT Grant IG100814.Peer Reviewe

Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells

Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.Fil: Wu, Ling. University of California at Los Angeles; Estados UnidosFil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados UnidosFil: Kyupelyan, Levon. University of California at Los Angeles; Estados UnidosFil: Latour, Brooke. University of California at Los Angeles; Estados UnidosFil: Gonzalez, Stephanie. University of California at Los Angeles; Estados UnidosFil: Shah, Saumya. University of California at Los Angeles; Estados UnidosFil: Galic, Zoran. University of California at Los Angeles; Estados UnidosFil: Ge, Sundi. University of California at Los Angeles; Estados UnidosFil: Zhu, Yuhua. University of California at Los Angeles; Estados UnidosFil: Petrigliano, Frank A.. University of California at Los Angeles; Estados UnidosFil: Nsair, Ali. University of California at Los Angeles; Estados UnidosFil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Li, Xinmin. University of California at Los Angeles; Estados UnidosFil: Lyons, Karen M.. University of California at Los Angeles; Estados UnidosFil: Crooks, Gay M.. University of California at Los Angeles; Estados UnidosFil: McAllister, David R.. University of California at Los Angeles; Estados UnidosFil: Van Handel, Ben. Novogenix Laboratories; Estados UnidosFil: Adams, John S.. University of California at Los Angeles; Estados UnidosFil: Evseenko, Denis. University of California at Los Angeles; Estados Unido