Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology

Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes

An efficient synthesis and physico-chemical properties of 2'-O-D-ribofuranosylnucleosides, minor tRNA components

A high yield preparation of 9-(2-O-beta-D-ribofuranosyl-beta-D-ribofuranosyl)adenine, guanine- and the pyrimidine analogs (cytosine, thymine and uracil base moiety) has been achieved, and the conformational properties of the ring systems were investigated using NMR spectroscopy and X-ray.status: publishe

An efficient synthesis and physico-chemical properties of 2'-O-D-ribofuranosylnucleosides, minor tRNA components.

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[Synthesis, Structure and Some Biochemical-properties of 3'-branched Thymidines and Their 5'-phosphate Derivatives]

A full scheme of synthesizing 3'-C-methyl-2'-deoxynucleosides and 3'-C-methylidene-28,3'-dideoxythymidine has been developed by using 2-deoxyribose. The stereoselectivity of the Grignard reagent's attachment to 2-deoxyfuranose 3-ulosides determined by the substitute configuration at Cl and the condensation stereoselectivity of 3-C-methyl-2-deoxyfuranosides with silylated thymine dependent on the configuration of the hydroxyl or-OBz group at C3 have been studied. The structure of the resultant compounds has been evidenced by H-1 and C-13 NMR, UV spectroscopies and C, H, and N analysis. The C2'-endo-C1-exo-conformation, the anti-conformation of the thymine base in relation to the glycoside bond and the gosh+-conformation in relation to the C4'-C5' bond are characteristic of the structure of 3'-C-methyl-2'-deoxythymidine in the crystal. 3'-C-Metyl-2'-deoxythymidine-5'-triphosphate exhibited the properties of the competitive inhibitor against 2'-deoxythimidine 5'-triphosphate in the synthesis of DNA catalyzed by various DNA-polymerases and reverse transcriptases. But none of these enzymes incorporated this compound into the growing DNA chain. At the same time 3'-C-methylidene-2',3'-dideoxythymidine-5'-triphosphate was incorporated into the 3'-end of the chain of DNA catalyzed by HIV reverse transcriptase, though the latter having a low efficacy. 3'-C-Methyl-2'-deoxythymidine failed to suppress HIV-1 production in the cultured MT-4 cells, its 5'-phosphite exhibiting a low activity under the same conditions

Verfahrenstechnische Untersuchung der Aufreinigung von monoklonalen Antikörpern mittels eines neuen Membranverfahrens

This chapter provides an overview of fluorescent labelling of different reactants related to the biochemistry of motor proteins. The fluorescent properties of different labels and the advantages and disadvantages of the labelling methods are discussed. This will allow for a careful selection of fluorescent proteins for different applications relating to motor proteins