AGAPEROS: Searching for variable stars in the LMC Bar with the Pixel Method. I. Detection, astrometry and cross-identification

We extend the work developed in previous papers on microlensing with a selection of variable stars. We use the Pixel Method to select variable stars on a set of 2.5 x 10**6 pixel light curves in the LMC Bar presented elsewhere. The previous treatment was done in order to optimise the detection of long timescale variations (larger than a few days) and we further optimise our analysis for the selection of Long Timescale and Long Period Variables (LT&LPV). We choose to perform a selection of variable objects as comprehensive as possible, independent of periodicity and of their position on the colour magnitude diagram. We detail the different thresholds successively applied to the light curves, which allow to produce a catalogue of 632 variable objects. We present a table with the coordinate of each variable, its EROS magnitudes at one epoch and an indicator of blending in both colours, together with a finding chart. A cross-correlation with various catalogues shows that 90% of those variable objects were undetected before, thus enlarging the sample of LT&LPV previously known in this area by a factor of 10. Due to the limitations of both the Pixel Method and the data set, additional data -- namely a longer baseline and near infrared photometry -- are required to further characterise these variable stars, as will be addressed in subsequent papers.Comment: 11 pages with 10 figure

Atomic transfers between implanted bioceramics and tissues in orthopaedics surgery

We study transfers of ions and debris from bioceramics implanted in bone sites. A contamination of surrounding tissues may play a major role in aseptic loosening of the implant. For these reasons, bioceramics require studies of biocompatibility and biofunctionality . So, in addition to in vitro studies of bioceramics, it is essential to implant them in vivo to know body reactions. We measured the concentration of mineral elements at different time intervals after implantation over a whole cross-section. We found a discontinuity of the mineral elements (Ca, P, Sr, Zn, Fe) at the interface between the implant and the receiver. The osseous attack is not global but, on the contrary, centripetal. Moreover, the fit of the concentration time course indicates that the kinetics of ossification is different for each atomic element and characterizes a distinct biological phenomeno

DENIS-P J104814.7-395606.1: An M9 dwarfs at about 4 pc

We present the discovery of a previously unknown member of the immediate solar neighbourhood, DENIS-P J104814.7-395606.1 (hereafter DENIS 1048-39), identified while mining the DENIS database for new nearby stars. A HIRES echelle spectrum obtained with the 10-m Keck telescope shows that it is an M9 dwarf. DENIS 1048-39 has a very bright apparent magnitude (I=12.67) for its spectral type and colour (I-J=3.07), and is therefore very nearby. If it is single its distance is only 4.1 +- 0.6pc, ranking it as between our twelfth and fortyth closest neighbour. It is also the closest star or brown dwarf with a spectral type later than M7V. Its proper motion was determined through comparison of Sky atlas Schmidt plates, scanned by the MAMA microdensitometer, with the DENIS images. At 1.53"/yr it further attests the closeness of DENIS 1048-39, and hence its dwarf status. These characteristics make it an obvious target for further detailed studies.Comment: In press in A&A Letter

Determination of proper motions and membership of the open star cluster NGC2548

Absolute proper motions, their corresponding errors and membership probabilities of 501 stars in the intermediate-age open cluster NGC2548 region are determined from MAMA measurements of 10 photographic plates. The plates have the maximum epoch difference of 82 years and they were taken with the double astrograph at Zo-Se station of Shanghai Observatory, which has an aperture of 40 cm and a plate scale of 30"/mm. The average proper motion precision is 1.18 mas/yr. These proper motions are used to determine the membership probabilities of stars in the region. The number of stars with membership probabilities higher than 0.7 is 165

Subzero nonfreezing storage of rat hepatocytes using UW solution and 1,4-butanediol. II- functional testing on rewarming and gene expression of urea cycle enzymes

In the present study we have analyzed the viability and metabolic competence of isolated rat hepatocytes subjected first, to subzero nonfreezing storage (up to 120 h at -4 oC) in modified University of Wisconsin (UW) solution with 8% 1,4-butanediol, and then to a normothermic rewarming step (KHR media, 37 oC, up to 120 min, carbogen atmosphere). Results were compared with hepatocytes stored up to 120 h at 0oC in modified UW solution and with freshly isolated hepatic cells. We have found that only cell suspensions stored in subzero nonfreezing conditions were able to finish the rewarming period with a viability comparable with the control group. Also, we have investigated the enzyme activities and the relative expression at messenger RNAs levels of two of the Urea cycle (UC) enzymes: Carbamyl phosphate synthetase I (CPSI) and ornithine transcarbamylase (OTC), during 60 min of rewarming. Results were compared with the ammonium removal efficiency of the three groups. In conclusion: These data indicated that hepatocytes preserved under cold or subzero conditions up to 120 h followed by 60 min of rewarming, maintain UC enzymes at levels similar to freshly isolated hepatocytes, allowing their use in bioartificial liver devices