2,077 research outputs found
Specific postgraduate programs and the transfer of the Panama Canal
This research examines the relevance and context of postgraduate programs offered by the University of Panama, specifically focusing on the âMasterâs Program in Maritime Industries with an Emphasis on Port Management, Multimodal Transportation, and Logisticsâ offered by the Institute of the Canal and International Studies. The study employs Guillermo Brionesâ specific reference model and adapts the âEvaluation Guide for Postgraduate Programsâ from the Ibero American University Postgraduate Association (AUIP). The analysis centers on three dimensions: Academic, linkage, and geographical-political. The results reveal a robust academic relationship between undergraduate and postgraduate programs, with high approval rates from coordinators (66.7%) and students (60%). Regarding linkage, the program demonstrates significant engagement with the business-productive sector, as 40% of respondents indicated. It also exhibits a favorable consideration of international trade rules, with 100% of coordinators affirming that program content incorporates international trade treaties. Furthermore, program graduates reported working in fields related to their education, with more definitive and positive responses than coordinators. This suggests a potential lack of data and ongoing connection between graduates and the institution that provided the program
Comparison of Two Lidar Methods of Wind Measurement by Cloud Tracking
We measured the horizontal wind speed vector with two separate lidar cloud tracking techniques. Data were taken during two measurement campaigns: HOLO-1, at Utah State University (USU), Utah, and HOLO-2 at St. Anselm College, New Hampshire. Army Research Office Lidar (AROL-2), Holographic Airborne Rotating Lidar Instrument (HARLIE), and a wide-angle camera were used during HOLO-1. Prototype Holographic Atmospheric Scanner for Environmental Remote Sensing (PHASERS) also participated in HOLO-2. Two measurement methods are described, and selected results from the two HOLO campaigns are shown
Optical Light Curve of the Type Ia Supernova 1998bu in M96 and the Supernova Calibration of the Hubble Constant
We present the UBVRI light curves of the Type Ia supernova SN 1998bu which
appeared in the nearby galaxy M96 (NGC 3368). M96 is a spiral galaxy in the Leo
I group which has a Cepheid-based distance. Our photometry allows us to
calculate the absolute magnitude and reddening of this supernova. These data,
when combined with measurements of the four other well-observed supernovae with
Cepheid based distances, allow us to calculate the Hubble constant with respect
to the Hubble flow defined by the distant Calan/Tololo Type Ia sample. We find
a Hubble constant of 64.0 +/- 2.2(internal) +/- 3.5(external) km/s/Mpc,
consistent with most previous estimates based on Type Ia supernovae. We note
that the two well-observed Type Ia supernovae in Fornax, if placed at the
Cepheid distance to the possible Fornax spiral NGC 1365, are apparently too
faint with respect to the Calan/Tololo sample calibrated with the five Type Ia
supernovae with Cepheid distances to the host galaxies.Comment: AAS LaTeX, 20 pages, 4 figures, 6 tables, accepted for publication in
the Astronomical Journal. Figure 1 (finding chart) not include
Carcinoma neuroendocrino de tiroides calcitonina negativa: Reporte de caso y revisiĂłn de la literatura
Medullary thyroid carcinoma (MTC) is a relatively rare neuroendocrine tumor that originates in the parafollicular C cells of the thyroid gland. It is characterized by the synthesis and secretion of calcitonin. Usually, serum calcitonin is used as part of the diagnosis and follow-up of these patients. Few cases of MTC with negative calcitonin have been reported worldwide, whose diagnosis is a clinical challenge.El carcinoma medular de tiroides (MTC) es un tumor neuroendocrino relativamente raro que se origina en las cĂ©lulas C para foliculares de la glĂĄndula tiroides. Se caracteriza por la sĂntesis y secreciĂłn de calcitonina. Por lo general, la calcitonina sĂ©rica se utiliza como parte del diagnĂłstico y seguimiento de estos pacientes. Se han notificado pocos casos de MTC con calcitonina negativa en todo el mundo, cuyo diagnĂłstico es un desafĂo clĂnico
Papilomatosis mĂșltiple de la mama. A propĂłsito de un caso
We present the case of a patient who comes to the Breast Oncology Surgery Service for presenting a small palpable lump in the right breast associated with nipple discharge. Mammography, breast ultrasound and magnetic resonance imaging workup revealed multiple nodular lesions in the right breast associated with ductal ectasia that compromised a large volume of breast. A single lesion suspicious for malignancy, was found in the left breast. The clinical and imaging diagnosis was multiple papillomatosis, findings that were confirmed by percutaneous biopsies of the most representative nodules in the right breast and the single lesion in the left breast. The importance of presenting this case lies in the fact that, multiple papillomatosis is an infrequent pathology, considered a high-risk lesion, with the possibility of being associated with a malignant neoplasm or becoming malignant over time, and there is no defined consensus for its management and follow-up.Se presenta el caso de una paciente que acude al Servicio de CirugĂa OncolĂłgica de Mamas, por presentar un pequeño tumor palpable en mama derecha asociado a telorrea. En los exĂĄmenes diagnĂłsticos de mamografĂa, ecografĂa mamaria y resonancia magnĂ©tica, se evidenciaron mĂșltiples lesiones nodulares en mama derecha asociados a ectasia ductal que comprometĂan gran parte del tejido glandular. En la mama izquierda se encontrĂł una lesiĂłn Ășnica, sospechosa de malignidad. El diagnĂłstico clĂnico y por imĂĄgenes fue de papilomatosis mĂșltiple, hallazgos que fueron confirmados mediante biopsias percutĂĄneas de los nĂłdulos mĂĄs representativos en mama derecha y de la lesiĂłn Ășnica de mama izquierda. La importancia de presentar este caso es porque la papilomatosis mĂșltiple es una patologĂa infrecuente, considerada como lesiĂłn de alto riesgo, con posibilidad de estar asociada a neoplasia maligna o a malignizarse en el tiempo, no habiendo un consenso definido para su manejo y seguimiento
Development of COVID-19 monoclonal antibodies and recombinant proteins as reagents for biomedical research and diagnostic test
Since SARS-COV-2 virus spread worldwide and COVID-19 turned rapidly into a pandemic illness, the necessity for vaccines and diagnostic tests became crucial. The viral surface is decorated with Spike, the major antigenic determinant and main target for vaccine development. Within Spike, the receptor binding domain (RBD), constitutes the main target of highly neutralizing antibodies found in COVID-19 convalescent plasma. Besides vaccination, another important aspect of Spike (and RBD) is their use as immunogen for the development of poli- and monoclonal antibodies (mAbs) for therapeutic and diagnostic purposes. Here we report the development and preliminary biochemical characterization of a set of monoclonal antibodies against the Spike RBD domain along with the recombinant expression of two mayor COVID-19 protein reagents: the viral Spike RBD domain and the extracellular domain of the human receptor ACE2. RBD and the extracellular domain of ACE2 (aa 1-740) were obtained through transient gene transfection (TGE) in two different mammalian cell culture systems: HEK293T adherent monolayers and Expi293 suspension cultures. Due to its low cost and ease scale-up, all transfections were carried with polyethyleneimine (PEI). Expressed proteins were purified from culture supernatants by immobilized metal affinity chromatography. Anti-RBD mAbs were developed from two different immunization schemes: one aimed to elicit antibodies with viral neutralizing potential, and the other with the ability to recognize denatured RBD for routinary lab immunoassays. To achieve this, the first group of mice was immunized with RBD in aluminium salts (RBD/Al) and the other with RBD emulsified in Freunds adyuvant (RBD/FA). Polyclonal and monoclonal antibody reactivities against native or denatured RBD forms were then assessed by ELISA. Complete RBD denaturation was followed by intrinsic fluorescence spectral changes upon different physicochemical stress treatments. As expected, RBD/Al immunized mice developed an antibody response shifted to native RBD while those immunized with RBD/FA showed a high response against both forms of the protein. In accordance with the observed polyclonal response, RBD/FA derived mAbs recognize both, native and denatured RBD. On the contrary, hybridomas generated from the RBD/Al protocol mostly recognize RBD in its native state. Further ELISA binding assays revealed that all RBD/FA derived mAbs can form a trimeric complex with ACE2 and RBD, denoting they would not have viral neutralizing activity. ELISA competition assays with the RBD/ACE2 complex aimed to determine the neutralization potential of the RBD/Al derived mAbs are under way. Overall, the anti-Spike RBD mAbs and the recombinant RBD and ACE2 proteins presented here constitute valuable tools for diverse COVID-19 academic research projects and local immunity surveillance testing.Fil: Acuña Intrieri, M. E. Universidad Nacional de San Martin. Centro de Rediseño E Ingenieria de Proteinas.; ArgentinaFil: Deriane, M.A. Universidad Nacional de San Martin. Centro de Rediseño E Ingenieria de Proteinas.; ArgentinaFil: Miller, C.. Universidad Nacional de San Martin. Centro de Rediseño E Ingenieria de Proteinas.; ArgentinaFil: Czibener, Cecilia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de Investigaciones BiotecnolĂłgicas. Universidad Nacional de San MartĂn. Instituto de Investigaciones BiotecnolĂłgicas; ArgentinaFil: Correa, E.. No especifĂca;Fil: Cragnaz, L.. No especifĂca;Fil: Guerra, L.. No especifĂca;Fil: Rodriguez, S.. No especifĂca;Fil: Goldbaum, F.A.. Universidad Nacional de San Martin. Centro de Rediseño E Ingenieria de Proteinas.; ArgentinaFil: Seigelchifer, M.. No especifĂca;Fil: Comerci, Diego JosĂ©. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de Investigaciones BiotecnolĂłgicas. Universidad Nacional de San MartĂn. Instituto de Investigaciones BiotecnolĂłgicas; ArgentinaFil: Montagna, Georgina Nuri. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de Investigaciones BiotecnolĂłgicas. Universidad Nacional de San MartĂn. Instituto de Investigaciones BiotecnolĂłgicas; ArgentinaFil: Cerutti, Maria Laura. Universidad Nacional de San Martin. Centro de Rediseño E Ingenieria de Proteinas.; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research y XVI Annual Meeting of the Argentinean Society for General MicrobiologyVirtualArgentinaSociedad Argentina de InvestigaciĂłn BioquĂmica y BiologĂa MolecularAsociaciĂłn Civil de MicrobiologĂa Genera
The Gray Needle: Large Grains in the HD 15115 Debris Disk from LBT/PISCES/Ks and LBTI/LMIRcam/L' Adaptive Optics Imaging
We present diffraction-limited \ks band and \lprime adaptive optics images of
the edge-on debris disk around the nearby F2 star HD 15115, obtained with a
single 8.4 m primary mirror at the Large Binocular Telescope. At \ks band the
disk is detected at signal-to-noise per resolution element (SNRE) \about 3-8
from \about 1-2\fasec 5 (45-113 AU) on the western side, and from \about
1.2-2\fasec 1 (63-90 AU) on the east. At \lprime the disk is detected at SNRE
\about 2.5 from \about 1-1\fasec 45 (45-90 AU) on both sides, implying more
symmetric disk structure at 3.8 \microns . At both wavelengths the disk has a
bow-like shape and is offset from the star to the north by a few AU. A surface
brightness asymmetry exists between the two sides of the disk at \ks band, but
not at \lprime . The surface brightness at \ks band declines inside 1\asec
(\about 45 AU), which may be indicative of a gap in the disk near 1\asec. The
\ks - \lprime disk color, after removal of the stellar color, is mostly grey
for both sides of the disk. This suggests that scattered light is coming from
large dust grains, with 3-10 \microns -sized grains on the east side and 1-10
\microns dust grains on the west. This may suggest that the west side is
composed of smaller dust grains than the east side, which would support the
interpretation that the disk is being dynamically affected by interactions with
the local interstellar medium.Comment: Apj-accepted March 27 2012; minor correction
Mycobacterium ulcerans Disease, Peru
Eight adult patients with Buruli ulcer were seen in a recent 10-year period
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