Large and variable genome size unrelated to serpentine adaptation but supportive of cryptic sexuality in Cenococcum geophilum

Estimations of genome size and its variation can provide valuable information regarding the genetic diversity of organisms and their adaptation potential to heterogeneous environments. We used flow cytometry to characterize the variation in genome size among 40 isolates of Cenococcum geophilum, an ectomycorrhizal fungus with a wide ecological and geographical distribution, obtained from two serpentine and two non-serpentine sites in Portugal. Besides determining the genome size and its intraspecies variation, we wanted to assess whether a relationship exists between genome size and the edaphic background of the C. geophilum isolates. Our results reveal C. geophilum to have one of the largest genome sizes so far measured in the Ascomycota, with a mean haploid genome size estimate of 0.208 pg (203 Mbp). However, no relationship was found between genome size and the edaphic background of the sampled isolates, indicating genetic and demographic processes to be more important for shaping the genome size variation in this species than environmental selection. The detection of variation in ploidy level among our isolates, including a single individual with both presumed haploid and diploid nuclei, provides supportive evidence for a possible cryptic sexual or parasexual cycle in C. geophilum (although other mechanisms may have caused this variation). The existence of such a cycle would have wide significance, explaining the high levels of genetic diversity and likelihood of recombination previously reported in this species, and adds to the increasing number of studies suggesting sexual cycles in previously assumed asexual fungi.This work was supported by research grant PTDC/BIA-BEC/100733/2008, funded by the European Union and the Fundação para a Ciência e a Tecnologia, awarded to Ludo Muller

Cell membrane and bioactive factors derived from mesenchymal stromal cells: Cell-free based therapy for inflammatory bowel diseases

Inflammatory bowel diseases (IBD) are chronic inflammatory disorders of the gastrointestinal tract associated with multifactorial conditions such as ulcerative colitis and Crohn's disease. Although the underlying mechanisms of IBD remain unclear, growing evidence has shown that dysregulated immune system reactions in genetically susceptible individuals contribute to mucosal inflammation. However, conventional treatments have been effective in inducing remission of IBD but not in preventing the relapse of them. In this way, mesenchymal stromal cells (MSC) therapy has been recognized as a promising treatment for IBD due to their immunomodulatory properties, ability to differentiate into several tissues, and homing to inflammatory sites. Even so, literature is conflicted regarding the location and persistence of MSC in the body after transplantation. For this reason, recent studies have focused on the paracrine effect of the biofactors secreted by MSC, especially in relation to the immunomodulatory potential of soluble factors (cytokines, chemokines, and growth factors) and extracellular vehicles that are involved in cell communication and in the transfer of cellular material, such as proteins, lipids, and nucleic acids. Moreover, treatment with interferon-γ, tumor necrosis factor-α, and interleukin- 1β causes MSC to express immunomodulatory molecules that mediate the suppression via cell-contact dependent mechanisms. Taken together, we present an overview of the role of bioactive factors and cell membrane proteins derived from MSC as a cell-free therapy that can improve IBD treatment

Non-linear QCD dynamics in two-photon interactions at high energies

Perturbative QCD predicts that the growth of the gluon density at high energies should saturate, forming a Color Glass Condensate (CGC), which is described in mean field approximation by the Balitsky-Kovchegov (BK) equation. In this paper we study the $\gamma \gamma$ interactions at high energies and estimate the main observables which will be probed at future linear colliders using the color dipole picture. We discuss in detail the dipole - dipole cross section and propose a new relation between this quantity and the dipole scattering amplitude. The total $\gamma \gamma$, $\gamma^{*} \gamma^{*}$ cross-sections and the real photon structure function $F_2^{\gamma}(x,Q^2)$ are calculated using the recent solution of the BK equation with running coupling constant and the predictions are compared with those obtained using phenomenological models for the dipole-dipole cross section and scattering amplitude. We demonstrate that these models are able to describe the LEP data at high energies, but predict a very different behavior for the observables at higher energies. Therefore we conclude that the study of $\gamma \gamma$ interactions can be useful to constrain the QCD dynamics.Comment: 11 pages, 5 figures. Version to be published in European Physical Journal

Semigroup Closures of Finite Rank Symmetric Inverse Semigroups

We introduce the notion of semigroup with a tight ideal series and investigate their closures in semitopological semigroups, particularly inverse semigroups with continuous inversion. As a corollary we show that the symmetric inverse semigroup of finite transformations $\mathscr{I}_\lambda^n$ of the rank $\leqslant n$ is algebraically closed in the class of (semi)topological inverse semigroups with continuous inversion. We also derive related results about the nonexistence of (partial) compactifications of classes of semigroups that we consider.Comment: With the participation of the new coauthor - Jimmie Lawson - the manuscript has been substantially revised and expanded. Accordingly, we have also changed the manuscript titl

Dipole model for double meson production in two-photon interactions at high energies

In this work the double vector meson production in two-photon interactions at high energies is investigated considering saturation physics. We extend the color dipole picture for this process and study the energy and virtuality dependence of the forward differential cross section. Comparison with previous results is presented and the contribution of the different photon polarizations is estimated.Comment: 20 pages, 8 figures, 1 table. Version to be published in European Physical Jornal

Unitarity Corrections to the Proton Structure Functions through the Dipole Picture

We study the dipole picture for the description of the deep inelastic scattering, focusing on the structure functions which are driven directly by the gluon distribution. One performs estimates using the effective dipole cross section given by the Glauber-Mueller approach in QCD, which encodes the corrections due to the unitarity effects associated with the saturation phenomenon. We also address issues about frame invariance of the calculations when analysing the observables.Comment: 16 pages, 8 figures. Version to be published in Phys. Rev.

Non-linear QCD dynamics and exclusive production in epep collisions

The exclusive processes in electron-proton ($ep$) interactions are an important tool to investigate the QCD dynamics at high energies as they are in general driven by the gluon content of proton which is strongly subject to parton saturation effects. In this paper we compute the cross sections for the exclusive vector meson production as well as the deeply virtual Compton scattering (DVCS) relying on the color dipole approach and considering the numerical solution of the Balitsky-Kovchegov equation including running coupling corrections. We show that the small-$x$ evolution given by this evolution equation is able to describe the DESY-HERA data and is relevant for the physics of the exclusive observables in future electron-proton colliders and in photoproduction processes to be measured in coherent interactions at the LHC.Comment: 6 pages, 4 figure

Physics of Seismo-electromagnetic Phenomena

The seismo-electromagnetic phenomena (SEMG) are integrated in a relatively recent research field that studies diverse phenomena such as: unusual seismo-electrical signals [1], abnormal ultra-low-frequency (ULF) seismo-electromagnetic emissions [2], very-low-frequency (VLF) and low-frequency (LF) radiobroadcast anomalies associated with ionosphere perturbations [3], variation of total electron content of the ionosphere [4], and atypical infrared emissions [5], all related with the preparatory stage of impending earthquakes. In the past, like many other branches of science like Quantum Mechanics, SEMG have been responsible for intense debates about its credibility, in this case concerning its applicability to short-term earthquake prediction [6]. In fact, the development of a truly pre-quake forecasting system is still an elusive plan, but SEM emissions are now a very well established effect extensively reported in literature. Nevertheless, much of the Physics implicated is still not fully understood. Thus, our main effort is directed towards a systematic field observation of SEMG effects and the development of both constructive theoretical models and laboratorial experiments to promote a better understanding of the Physics engaged in these phenomena. In this presentation we will present a sum up of our recent achievements [7,8,9], focusing future work and improvements. [1] A. Konstantaras, et al., On the electric field transient anomaly observed at the time of the Kythira M=6.9 earthquake on January 2006, Nat. Hazards Earth Syst. Sci. 7, 677 (2007). [2] T. Bleier, et al., Investigation of ULF magnetic pulsations, air conductivity changes, and infra red signatures associated with the 30 October Alum Rock M5.4 earthquake, Nat. Hazards Earth Syst. Sci. 9, 585 (2009). [3] P. Biagi, et al., An overview on preseismic anomalies in LF radio signals revealed in Italy by wavelet analysis, Annals of Geophysics 51, 237 (2008). [4] V. Chauhan, et al., Ultra-low-frequency (ULF) and total electron content (TEC) anomalies observed at Agra and their association with regional earthquakes, Journal of Geodynamics 48, 68 (2009). [5] D. Ouzounov, et al., Outgoing long wave radiation variability from IR satellite data prior to major earthquakes, Tectonophysics 431, 211 (2007). [6] S. Uyeda, et al., Short-term earthquake prediction: Current status of seismo-electromagnetics, Tectonophysics 470, 205 (2009). [7] H.G. Silva, et al., Atmospheric electrical field anomalies associated with seismic activity, Nat. Hazards Earth Syst. Sci. 11, 987 (2011). [8] H. G. Silva, et al., Electric transport in different granitic rocks, EGU General Assembly 2011 (EGU 2011), 3-8 April 2011, Vienna (Austria). [9] H.G. Silva, et al., Piezoelectric effect during solid fracture causing electromagnetic emissions, International Conference on Computational Modelling of Fracture and Failure (CFRAC 2011), 6-8 June 2011, Barcelona (Spain)