1,175 research outputs found
Weevil resistant sweetpotato through biotechnology.
This flyer summarizes the objectives, achievements and plans of CIP’s work to address the problem of weevils through development of weevil resistant sweetpotato varieties through breeding and biotechnology. It outlines the Bt-RNAi combined gene technology being applied, and the progress made between 2014 and 2015 towards achieving the project objectives
Ex-post analysis of landraces sympatric to a commercial variety in the center of origin of the potato failed to detect gene flow.
Genetic transformatin of potato with a triple R gene construct to confer resistance to late blight.
The cultivated potato, Solanum tuberosum is affected by a variety of diseases with late blight (LB) caused by Phytophthora infestans being the most severe. The disease is mostly controlled by the application of large quantities of fungicides, which represent a financial burden on farmers indeveloping countries and pose risks to both human health and the environment. A more effective and environmentally friendly strategy to prevent damages caused by P. infestans is to use resistant potato cultivars. In the early days of breeding for LB resistance, a small number of resistance (R) genes from the wild Mexican species, Solanum demissum, were introgressed into modern potato varieties. These genes conferred race-specific resistance, which was rapidly overcome by new isolates of the pathogen. Recently, a number of new R genes have been identified and cloned from several wild potato species .Taking advantage of genetic engineering, our strategy is to use three of these new R genes (RB, Rpiblb2 and Rpi-vnt1.1) in a triple gene construct, p CIP 99, based on the hypothesis that simultaneous mutation for pathogenicity against all three genes is unlikely, and therefore the resistance conferred by the construct should be durable. We plan to deploy the three stacked R genes into the potato variety “Victoria” (known in Kenya and Uganda as “Asante”). To date, we have produced more than 100 transgenic events which are currently being characterized. The pathogen population will be characterized concurrently to assess the expected durability of this resistance
Prevalence of Gestational Diabetes in Hospitals in the City of Lubumbashi in the Democratic Republic of Congo: Case of the Jason Sendwe General Reference Hospital in 2015 to 2019
Gestational diabetes is defined as a carbohydrate tolerance disorder leading to hyperglycaemia of varying severity, occurring or first diagnosed during pregnancy, regardless of the treatment required and the course of the postpartum period. Its prevalence has been estimated at between 2.2% and 8.8% of pregnancies, depending on the populations studied and the screening criteria used. We determined the prevalence of gestational diabetes at the Jason Sendwe General Referral Hospital. A descriptive cross-sectional study was carried out from January 2015 to December 2019 at Sendwe Hospital. It concerned 48 pregnant women from 24 weeks of pregnancy. The diagnosis of gestational diabetes was made by blood glucose obtained with the O'Sullivan test or by fasting blood glucose. The prevalence of gestational diabetes was 2.15%. Modifiable and non-modifiable factors associated with gestational diabetes in this study are age ≥ 35 years, parity, history of macrosomia, and history of type 2 diabetes in the surrogates of the pregnancies. The prevalence rate remains high and shows that gestational diabetes is a public health problem in the city of Lubumbashi. The O'Sullivan test is a valid alternative for its good detection. We found that age, especially after 35 years is the major determinant of gestational diabetes in the population living in Lubumbashi
Organization of phenylalanine ammonia lyase (PAL), acidic PR-5 and osmotin-like (OSM) defence-response gene families in the potato genome
Exploiting the use of biotechnology in sweetpotato for improved nutrition and food security: Progress and future outlook.
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