Effect of hair shearing on live performance and carcass traits of growing rabbits under hot ambient temperature

The aim of the study was to examine the effect of hair shearing in growing rabbits reared at high ambient temperature. The live performance and carcass traits of growing rabbits reared at 20°C (not sheared, C, n=50) or at 28°C (not sheared, H, n=50, or sheared at 5, 7 and 9 wk, HS, n=50) were compared. The ambient temperature and relative humidity were 20.5±1.1°C and 54±11% in the 20°C room and 28.8±0.2°C and 35±8% in 28°C room, respectively. Feed intake of H and HS groups decreased by 29.0 and 20.4%, respectively, compared to C rabbits (P<0.001). The same data for weight gain were 24.6 and 16.9% (P<0.001), and for body weight at 12 wk were 16.8 and 11.5% (P<0.001). At the same time, the feed conversion ratio improved (C: 3.53, HS: 3.34, H: 3.31; P<0.001). Nevertheless, the mortality rate of rabbits was not affected by the studied treatment and was overall low (0-4%). No differences were observed in dressing out percentages either (ratio of chilled carcass (CC) to the slaughter weight: 61.6-61.9%). The ratio of liver to CC differed among the experimental groups, with the highest value recorded in C group and the lowest in H group; HS rabbits showed intermediate results (C: 4.86%, HS: 4.27%, H: 3.91%; P<0.001). Lower ratios of fat deposits to reference carcass were also observed in rabbits kept at high ambient temperature (perirenal fat: C: 2.59%, HS: 1.82%, H: 1.60%; P<0.001; scapular fat: C: 0.89%, HS: 0.66%, H: 0.51%; P<0.001). It can be concluded that the negative effect of higher ambient temperature (28 vs. 20°C) on production in growing rabbits can be reduced significantly by hair shearing

Exclusive neuronal expression of SUCLA2 in the human brain

SUCLA2 encodes the ATP-forming subunit (A-SUCL-) of succinyl-CoA ligase, an enzyme of the citric acid cycle. Mutations in SUCLA2 lead to a mitochondrial disorder manifesting as encephalomyopathy with dystonia, deafness and lesions in the basal ganglia. Despite the distinct brain pathology associated with SUCLA2 mutations, the precise localization of SUCLA2 protein has never been investigated. Here we show that immunoreactivity of A-SUCL- in surgical human cortical tissue samples was present exclusively in neurons, identified by their morphology and visualized by double labeling with a fluorescent Nissl dye. A-SUCL- immunoreactivity co-localized >99% with that of the d subunit of the mitochondrial F0-F1 ATP synthase. Specificity of the anti-A-SUCL- antiserum was verified by the absence of labeling in fibroblasts from a patient with a complete deletion of SUCLA2. A-SUCL- immunoreactivity was absent in glial cells, identified by antibodies directed against the glial markers GFAP and S100. Furthermore, in situ hybridization histochemistry demonstrated that SUCLA2 mRNA was present in Nissl-labeled neurons but not glial cells labeled with S100. Immunoreactivity of the GTP-forming subunit (G-SUCL-) encoded by SUCLG2, or in situ hybridization histochemistry for SUCLG2 mRNA could not be demonstrated in either neurons or astrocytes. Western blotting of post mortem brain samples revealed minor G-SUCL- immunoreactivity that was however, not upregulated in samples obtained from diabetic versus non-diabetic patients, as has been described for murine brain. Our work establishes that SUCLA2 is expressed exclusively in neurons in the human cerebral cortex

Alterations in voltage-sensing of the mitochondrial permeability transition pore in ANT1-deficient cells

The probability of mitochondrial permeability transition (mPT) pore opening is inversely related to the magnitude of the proton electrochemical gradient. The module conferring sensitivity of the pore to this gradient has not been identified. We investigated mPT's voltage-sensing properties elicited by calcimycin or H2O2 in human fibroblasts exhibiting partial or complete lack of ANT1 and in C2C12 myotubes with knocked-down ANT1 expression. mPT onset was assessed by measuring in situ mitochondrial volume using the 'thinness ratio' and the 'cobalt-calcein' technique. De-energization hastened calcimycin-induced swelling in control and partially-expressing ANT1 fibroblasts, but not in cells lacking ANT1, despite greater losses of mitochondrial membrane potential. Matrix Ca(2+) levels measured by X-rhod-1 or mitochondrially-targeted ratiometric biosensor 4mtD3cpv, or ADP-ATP exchange rates did not differ among cell types. ANT1-null fibroblasts were also resistant to H2O2-induced mitochondrial swelling. Permeabilized C2C12 myotubes with knocked-down ANT1 exhibited higher calcium uptake capacity and voltage-thresholds of mPT opening inferred from cytochrome c release, but intact cells showed no differences in calcimycin-induced onset of mPT, irrespective of energization and ANT1 expression, albeit the number of cells undergoing mPT increased less significantly upon chemically-induced hypoxia than control cells. We conclude that ANT1 confers sensitivity of the pore to the electrochemical gradient

Alterations in voltage-sensing of the mitochondrial permeability transition pore in ANT1-deficient cells

The probability of mitochondrial permeability transition (mPT) pore opening is inversely related to the magnitude of the proton electrochemical gradient. The module conferring sensitivity of the pore to this gradient has not been identified. We investigated mPT's voltage-sensing properties elicited by calcimycin or H2O2 in human fibroblasts exhibiting partial or complete lack of ANT1 and in C2C12 myotubes with knocked-down ANT1 expression. mPT onset was assessed by measuring in situ mitochondrial volume using the 'thinness ratio' and the 'cobalt-calcein' technique. De-energization hastened calcimycin-induced swelling in control and partially-expressing ANT1 fibroblasts, but not in cells lacking ANT1, despite greater losses of mitochondrial membrane potential. Matrix Ca(2+) levels measured by X-rhod-1 or mitochondrially-targeted ratiometric biosensor 4mtD3cpv, or ADP-ATP exchange rates did not differ among cell types. ANT1-null fibroblasts were also resistant to H2O2-induced mitochondrial swelling. Permeabilized C2C12 myotubes with knocked-down ANT1 exhibited higher calcium uptake capacity and voltage-thresholds of mPT opening inferred from cytochrome c release, but intact cells showed no differences in calcimycin-induced onset of mPT, irrespective of energization and ANT1 expression, albeit the number of cells undergoing mPT increased less significantly upon chemically-induced hypoxia than control cells. We conclude that ANT1 confers sensitivity of the pore to the electrochemical gradient

Bioinformatic Characterization of P-Type ATPases Encoded Within the Fully Sequenced Genomes of 26 Eukaryotes

P-type ATPases play essential roles in numerous processes, which in humans include nerve impulse propagation, relaxation of muscle fibers, secretion and absorption in the kidney, acidification of the stomach and nutrient absorption in the intestine. Published evidence suggests that uncharacterized families of P-type ATPases with novel specificities exist. In this study, the fully sequenced genomes of 26 eukaryotes, including animals, plants, fungi and unicellular eukaryotes, were analyzed for P-type ATPases. We report the organismal distributions, phylogenetic relationships, probable topologies and conserved motifs of nine functionally characterized families and 13 uncharacterized families of these enzyme transporters. We have classified these proteins according to the conventions of the functional and phylogenetic IUBMB-approved transporter classification system (www.tcdb.org, Saier et al. in Nucleic Acids Res 34:181–186, 2006; Nucleic Acids Res 37:274–278, 2009)

Coral Uptake of Inorganic Phosphorus and Nitrogen Negatively Affected by Simultaneous Changes in Temperature and pH

The effects of ocean acidification and elevated seawater temperature on coral calcification and photosynthesis have been extensively investigated over the last two decades, whereas they are still unknown on nutrient uptake, despite their importance for coral energetics. We therefore studied the separate and combined impacts of increases in temperature and pCO2 on phosphate, ammonium, and nitrate uptake rates by the scleractinian coral S. pistillata. Three experiments were performed, during 10 days i) at three pHT conditions (8.1, 7.8, and 7.5) and normal temperature (26°C), ii) at three temperature conditions (26°, 29°C, and 33°C) and normal pHT (8.1), and iii) at three pHT conditions (8.1, 7.8, and 7.5) and elevated temperature (33°C). After 10 days of incubation, corals had not bleached, as protein, chlorophyll, and zooxanthellae contents were the same in all treatments. However, photosynthetic rates significantly decreased at 33°C, and were further reduced for the pHT 7.5. The photosynthetic efficiency of PSII was only decreased by elevated temperature. Nutrient uptake rates were not affected by a change in pH alone. Conversely, elevated temperature (33°C) alone induced an increase in phosphate uptake but a severe decrease in nitrate and ammonium uptake rates, even leading to a release of nitrogen into seawater. Combination of high temperature (33°C) and low pHT (7.5) resulted in a significant decrease in phosphate and nitrate uptake rates compared to control corals (26°C, pHT = 8.1). These results indicate that both inorganic nitrogen and phosphorus metabolism may be negatively affected by the cumulative effects of ocean warming and acidification

The Mitochondrial Targets of Neuroprotective Drug Vinpocetine on Primary Neuron Cultures, Brain Capillary Endothelial Cells, Synaptosomes, and Brain Mitochondria

Vinpocetine is considered as neuroprotectant drug and used for treatment of brain ischemia and cognitive deficiencies for decades. A number of enzymes, channels and receptors can bind vinpocetine, however the mechanisms of many effects' are still not clear. The present study investigated the effects of vinpocetine from the mitochondrial bioenergetic aspects. In primary brain capillary endothelial cells the purinergic receptor-stimulated mitochondrial Ca2+ uptake and efflux were studied. Vinpocetine exerted a partial inhibition on the mitochondrial calcium efflux. In rodent brain synaptosomes vinpocetine (30 μM) inhibited respiration in uncoupler stimulated synaptosomes and decreased H2O2 release from the nerve terminals in resting and in complex I inhibited conditions, respectively. In isolated rat brain mitochondria using either complex I or complex II substrates leak respiration was stimulated, but ADP-induced respiration was inhibited by vinpocetine. The stimulation of oxidation was associated with a small extent of membrane depolarization. Mitochondrial H2O2 production was inhibited by vinpocetine under all conditions investigated. The most pronounced effects were detected with the complex II substrate succinate. Vinpocetine also mitigated both Ca2+-induced mitochondrial Ca2+-release and Ca2+-induced mitochondrial swelling. It lowered the rate of mitochondrial ATP synthesis, while increasing ATPase activity. These results indicate more than a single mitochondrial target of this vinca alkaloid. The relevance of the affected mitochondrial mechanisms in the anti ischemic effect of vinpocetine is discussed

EFFECT OF DIETARY SUPPLEMENTATION OF CHESTUNT HYDROLISABLE TANNIN ON DIGESTIVE EFFICIENCY, GROWTH PERFORMANCE AND MEAT QUALITY IN GROWING RABBITS

The aim of the study was to evaluate the effect of the dietary supplementation of chestnut hydrolysable tannin on the feed digestibility and nutritive value, mortality and growth performance, fatty acid profile and TBARS of cooked Longissimus dorsi (LD) meat. From the age of 18 days the rabbits were fed pellets supplemented with coccidiostat (CC) or with tannin (400 g/100 kg: T400). At weaning (35 d of age) within both groups 5 dietary sub-groups were formed: medicated-free (C0), containing coccidiostatic (CC), or supplemented with increasing levels of chestnut tannin (T200, T400 and T600). Rabbits fed the 5 diets ad libitum until slaughter at 11 weeks of age. The coefficient of total tract apparent digestibility (CTTAD) of nutrients was not affected by the tannin inclusion, substantially, with exception of CTTAD of hemicelluloses, that was lower in T400 and T600 compared to C0 and T200 (P<0.001), the CTTAD of Ca, lower in T400 vs C0 and CC (P<0.05) and CTTAD of DP-to-DE ratio, lowest in CC and T600 (P<0.001). Dietary tannin significantly modified the CTTAD of FA classes. At weaning, the live weight of CC rabbits was significantly higher than that of the T400 rabbits (974 vs 940 g, P<0.05). The other variables related to growth performance, health status and carcass traits were not affected by the tannin supplementation. The T400 diet fed before weaning seemed to be more effective than the 3 tannin levels administered during fattening period on FA profile change in the LD muscle. T400 diet fed before weaning increased SFA and MUFA (P<0.01) and decreased PUFA (P<0.05), decreasing the n-6/n-3 ratio (P<0.01), however. When tannin-supplemented diets were fed during fattening, only T600 diet showed significantly higher (P<0.05) SFA and MUFA contents compared to CC diet. In conclusion, the dietary inclusion of chestnut hydrolysable tannin doesn\u2019t provides improvements in health status, diet nutritive value, growth performance and carcass traits. Dietary hydrolysable tannin supplementation may play a role on lipid metabolism

The effect of the feed supplemented by different tannin levels on the production and carcass traits of growing rabbits

The objective of the experiment was to analyze the production and carcass traits of growing rabbits fed by medicament free and by supplemented (with coccidiostaticum, or with 3 different tannin levels) pellet, respectively. From the age of 18 days the rabbits were fed by medicament free pellet supplemented by coccidiostaticum (Cc), or by tannin (400g/100kg: T400). At weaning (35 days of age) within both groups 5 subgroups were formed: medicament free (CO), and supplemented pellet containing coccidiostaticum (Cc), or different levels of tannin (Farmatan) (T200, T400 and T600). At weaning the body weight of the Cc rabbits was significantly higher than that of the T400 rabbits (974 vs. 940g, P<0.05). Except for this finding the fed pellet type did not influence the growing rabbits\u2019 production (weight gain, body weight, feed consumption, feed conversion ratio, mortality). The pellet type fed prior to weaning significantly affected the perirenal fat weight (Cc = 28.9 and T400 = 26.3 g; P<0.05). The rabbits fed by tannin supplemented pellet after weaning had a higher mid part ratio compared to the reference carcass (P = 0.1). As the mortality rate was low in all groups (CO, T200 and T600 = 5.9%, T400 = 1.4%, Cc = 0%, NS), it can be concluded that no positive effect can be expected when the analyzed population is healthy and the housing conditions and the feed compositions are adequate

Effect of feeding (only pellet or pellet plus hay) on productive and carcass traits of growing rabbits

The aim of the experiment was to examine the effect of feeding on productive performance and carcass traits, to get information about the difference between growing rabbits fed by only pellet and pellet plus hay. Pannon Ka does were inseminated with semen of Pannon Large and Hungarian Giant bucks. The crossbred rabbits (n=336) were weaned at 5 weeks of age. Half of them were housed in cages, the other half in pens (Cage: 3 rabbits/cage, Pen: 14 rabbits/pen, the stocking density was the same: 16 rabbits/m2 in each group). Two subgroups were formed based on the feeding method. Data (between 5 and 12 wk) were evaluated by multi-factor analysis of variance, but only the effect of feeding was calculated. The differences in body weight between Pellet and P+hay rabbits were significant from 9 weeks of age, in favour of Pellet group. It increased from 68 g at 9 weeks to 76 g at 12 weeks. The differences in weight gain and feed intake were significant between 5-12 weeks (11 and 1.3 g/day, respectively), in favour of Pellet rabbits. However, there were no significant differences in feed conversion ratio and mortality. The dressing out percentage was 0.4-0.7 % higher in Pellet group than in P+Hay rabbits. The ratio of hind part to reference carcass was higher in P+Hay group, and that of perirenal and scapular fat were higher in Pellet group. Feeding method did not influence the ratio of fore and mid parts to reference carcass. However, ratio of ear lesions was affected by feeding method (8.3 and 20.6 %; P=0.003, in pellet and P+hay, respectively). It can be concluded that feeding growing rabbits with pellet plus hay resulted significantly, but slightly lower body weight and longer fattening period to reach the same weight. On the other hand, animals fed by pellet plus hay realized less ear lesions