Effect of vine vigour of Vitis vinifera cv. Nebbiolo clones on wine acidity and quality

The grapevine cv. Nebbiolo grown in northern Italy produces high-quality red wines, of which Barolo and Barbaresco are the best known. During a clonal selection project, clones of this variety were assessed for their agronomical and enological value. Different degrees of vegetative vigour were found among them, and this was related to modifications of must and wine composition, with particular respect to the acidity. Over 4 years of observations, vigorous clones produced musts and wines of higher pH, regardless of the amount of titratable acidity. This was associated with a higher malic acid content in the juice and with a higher concentration of potassium in the wine. In addition, wines from vigorous clones showed an unbalanced ratio of colour components. They ranked at the lowest score in the sensory evaluation tests

Tc=21K in epitaxial FeSe0.5Te0.5 thin films with biaxial compressive strain

High purity epitaxial FeSe0.5Te0.5 thin films with different thickness were grown by Pulsed Laser Ablation on different substrates. By varying the film thickness, Tc up to 21K were observed, significantly larger than the bulk value. Structural analyses indicated that the a axis changes significantly with the film thickness and is linearly related to the Tc. The latter result indicates the important role of the compressive strain in enhancing Tc. Tc is also related to both the Fe-(Se,Te) bond length and angle, suggesting the possibility of further enhancement

Temperature- and doping-dependent nanoscale Schottky barrier height at the Au/Nb:SrTiO3interface

We use ballistic electron emission microscopy to investigate prototypical Au/Nb-doped SrTiO3(NSTO) Schottky barrier diodes for different temperatures and doping levels. To this end, ultrathin Au overlayers are thermally evaporated onto TiO2-terminated NSTO single crystal substrates. We show that at room temperature, regardless of the nominal doping, rectification is controlled by a spatially inhomogeneous Schottky barrier height (SBH), which varies on a length scale of tens of nanometers according to a Gaussian distribution with a mean value of 1.29-1.34 eV and the standard deviation in the range of 80-100 meV. At lower temperatures, however, doping effects become relevant. In particular, junctions with a low Nb content of 0.01 and 0.05 wt. % show an 3c300 meV decrease in the mean SBH from room temperature to 80 K, which can be explained by an electrostatic analysis assuming a temperature-dependent dielectric permittivity for NSTO. In contrast, this model fails to predict the weaker temperature dependence of SBH for junctions based on 0.5 wt. % NSTO. Our nanoscale investigation demands to reassess conventional models for the NSTO polarizability in high-intensity electric fields. Furthermore, it contributes to the comprehension and prediction of transport in metal/SrTiO3junctions and devices

William R. Brinkley:A giant in biomedical research and public policy

Susan A. Gerbi, Robert E. Palazzo, William C. Earnshaw, and William T. Schrader discuss the life and achievements of William R. Brinkley, who passed away on November 10, 2020

U4 snRNA nucleolar localization requires the NHPX/15.5-kD protein binding site but not Sm protein or U6 snRNA association

All small nuclear RNAs (snRNAs) of the [U4/U6.U5] tri-snRNP localize transiently to nucleoli, as visualized by microscopy after injection of fluorescein-labeled transcripts into Xenopus laevis oocyte nuclei. Here, we demonstrate that these RNAs traffic to nucleoli independently of one another, because U4 snRNA deleted in the U6 base-pairing region still localizes to nucleoli. Furthermore, depletion of endogenous U6 snRNA does not affect nucleolar localization of injected U4 or U5. The wild-type U4 transcripts used here are functional: they exhibit normal nucleocytoplasmic traffic, associate with Sm proteins, form the [U4/U6] di-snRNP, and localize to nucleoli and Cajal bodies. The nucleolar localization element (NoLE) of U4 snRNA was mapped by mutagenesis. Neither the 5′-cap nor the 3′-region of U4, which includes the Sm protein binding site, are essential for nucleolar localization. The only region in U4 snRNA required for nucleolar localization is the 5′-proximal stem loop, which contains the binding site for the NHPX/15.5-kD protein. Even mutation of just five nucleotides, essential for binding this protein, impaired U4 nucleolar localization. Intriguingly, the NHPX/15.5-kD protein also binds the nucleolar localization element of box C/D small nucleolar RNAs, suggesting that this protein might mediate nucleolar localization of several small RNAs

High quality epitaxial FeSe0.5Te0.5 thin films grown on SrTiO3 substrates by pulsed laser deposition

Superconducting epitaxial FeSe0.5Te0.5 thin films were prepared on SrTiO3 (001) substrates by pulsed laser deposition. The high purity of the phase, the quality of the growth and the epitaxy were studied with different experimental techniques: X-rays diffraction, reflection high energy electron diffraction, scanning tunnelling microscopy and atomic force microscopy. The substrate temperature during the deposition was found to be the main parameter governing sample morphology and superconducting critical temperature. Films obtained in the optimal conditions show an epitaxial growth with c axis perpendicular to the film surface and the a and b axis parallel to the substrates one, without the evidence of any other orientation. Moreover, such films show a metallic behavior over the whole measured temperature range and critical temperature above 17K, which is higher than the target one.Comment: 10 pages including 4 figure

Assessement of sensory firmness and crunchiness of table grapes by acoustic and mechanical properties.

Background and Aims The instrumental measurement of crunchiness in tablegrapes has been the subject of little research in spite of the great relevance of this sensory texture trait to consumer preference. Therefore, our aim was to evaluate the potential of several mechanical and acoustic properties to assess the perceived firmness and crunchiness of tablegrape cultivars. Methods and Results The ripening effect was minimised by densimetric sorting of the berries before testing. The textural quality of seven tablegrape cultivars was evaluated by sensory analysis. Furthermore, three mechanical tests (texture profile analysis, cutting and denture) were performed on the berry flesh or on whole berries, and the acoustic emission produced was recorded simultaneously. Correlation studies showed strong and significant relationships between sensory texture attributes and instrumental parameters, particularly for the denture test. Nevertheless, satisfactory predictive accuracy for the perceived crunchiness required multivariate linear regression involving both mechanical and acoustic properties resulting from the denture test performed on whole berries. In this case, residual predictive interquartile amplitude was higher than 2. Most of the reliable models developed for perceived firmness are fairly recommended not for quantitative purposes but for fast screening (1.6 < residual predictive interquartile amplitude < 2). Conclusions The standardised protocol proposed permits more objective and quantitative sensory data to be obtained for firmness and crunchiness of tablegrapes. Significance of the Study A combined mechanical–acoustic strategy has not previously been used in tablegrapes and represents a powerful tool for a more complete and exhaustive texture characterisation, particularly firmness and crunchiness, by means of a more objective and standardised protocol