Depletion of UDP-Glucose and UDP-Galactose Using a Degron System Leads to Growth Cessation of Leishmania major

Interconversion of UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal) by the UDP-Glc 4´-epimerase intimately connects the biosynthesis of these two nucleotide sugars. Their de novo biosynthesis involves transformation of glucose-6-phosphate into glucose-1-phosphate by the phosphoglucomutase and subsequent activation into UDP-Glc by the specific UDP-Glc pyrophosphorylase (UGP). Besides UGP, Leishmania parasites express an uncommon UDP-sugar pyrophosphorylase (USP) able to activate both galactose-1-phosphate and glucose-1-phosphate in vitro. Targeted gene deletion of UGP alone was previously shown to principally affect expression of lipophosphoglycan, resulting in a reduced virulence. Since our attempts to delete both UGP and USP failed, deletion of UGP was combined with conditional destabilisation of USP to control the biosynthesis of UDP-Glc and UDP-Gal. Stabilisation of the enzyme produced by a single USP allele was sufficient to maintain the steady-state pools of these two nucleotide sugars and preserve almost normal glycoinositolphospholipids galactosylation, but at the apparent expense of lipophosphoglycan biosynthesis. However, under destabilising conditions, the absence of both UGP and USP resulted in depletion of UDP-Glc and UDP-Gal and led to growth cessation and cell death, suggesting that either or both of these metabolites is/are essential

Octonionic Selfduality for SuperMembranes

In this work we study the recently introduced octonionic duality for membranes. Restricting the self - duality equations to seven space dimensions, we provide various forms for them which exhibit the symmetries of the octonionic and quaternionic structure. These forms may turn to be useful for the question of the integrability of this system. Introducing a consistent quadratic Poisson algebra of functions on the membrane we are able to factorize the time dependence of the self - duality equations. We further give the general linear embeddings of the three dimensional system into the seven dimensional one using the invariance of the self-duality equations under the exceptional group G_2.Comment: Latex file, 21 page

Euler Top Dynamics of Nambu-Goto P-Branes

We propose a method to obtain new exact solutions of spinning p-branes in flat space-times for any p, which manifest themselves as higher dimensional Euler Tops and minimize their energy functional. We provide concrete examples for the case of spherical topology S^{2}, S^{3} and rotational symmetry \prod_{i}SO(q_{i}). In the case of toroidal topology T^{2}, T^{3} the rotational symmetry is \prod SU(q_{i}) and m target dimensions are compactified on the torus T^{m} . By double dimensional reduction the Light Cone Hamiltonians of T^{2}, T^{3} reduce to those of closed string S^{1} and T^{2} membranes respectively. The solutions are interpreted as non-perturbative spinning soliton states of type IIA-IIB superstrings.Comment: 33 pages, LATEX; more typos corrected; some equation numbering correction

Astroparticle Physics with a Customized Low-Background Broad Energy Germanium Detector

The MAJORANA Collaboration is building the MAJORANA DEMONSTRATOR, a 60 kg array of high purity germanium detectors housed in an ultra-low background shield at the Sanford Underground Laboratory in Lead, SD. The MAJORANA DEMONSTRATOR will search for neutrinoless double-beta decay of 76Ge while demonstrating the feasibility of a tonne-scale experiment. It may also carry out a dark matter search in the 1-10 GeV/c^2 mass range. We have found that customized Broad Energy Germanium (BEGe) detectors produced by Canberra have several desirable features for a neutrinoless double-beta decay experiment, including low electronic noise, excellent pulse shape analysis capabilities, and simple fabrication. We have deployed a customized BEGe, the MAJORANA Low-Background BEGe at Kimballton (MALBEK), in a low-background cryostat and shield at the Kimballton Underground Research Facility in Virginia. This paper will focus on the detector characteristics and measurements that can be performed with such a radiation detector in a low-background environment.Comment: Submitted to NIMA Proceedings, SORMA XII. 9 pages, 4 figure

The SABRE project and the SABRE Proof-of-Principle

SABRE aims to directly measure the annual modulation of the dark matter interaction rate with NaI(Tl) crystals. A modulation compatible with the standard hypothesis, in which our Galaxy is immersed in a dark matter halo, has been measured by the DAMA experiment in the same target material. Other direct detection experiments, using different target materials, seem to exclude the interpretation of such modulation in the simplest scenario of WIMP-nucleon elastic scattering. The SABRE experiment aims to carry out an independent search with sufficient sensitivity to confirm or refute the DAMA claim. The goal of the SABRE experiment is to achieve the lowest background rate for a NaI(Tl) experiment (order of 0.1 cpd/kg/keVee in the energy region of interest for dark matter). This challenging goal could be achievable by operating high-purity crystals inside a liquid scintillator veto for active background rejection. In addition, twin detectors will be located in the northern and southern hemispheres to identify possible contributions to the modulation from seasonal or site-related effects. The SABRE project includes an initial Proof-of-Principle phase at LNGS (Italy), to assess the radio-purity of the crystals and the efficiency of the liquid scintillator veto. This paper describes the general concept of SABRE and the expected sensitivity to WIMP annual modulation.M. Antonello … A.G. Williams … et al

Town Of Great Barrington, Massachusetts Annual Reports For The Fiscal Year 2016 July 1, 2015 to June 30, 2016

SABRE (Sodium-iodide with Active Background REjection) is a direct dark matter search experiment based on an array of radio-pure NaI(Tl) crystals surrounded by a liquid scintillator veto. Twin SABRE experiments in the Northern and Southern Hemispheres will differentiate a dark matter signal from seasonal and local effects. The experiment is currently in a Proof-of-Principle (PoP) phase, whose goal is to demonstrate that the background rate is low enough to carry out an independent search for a dark matter signal, with sufficient sensitivity to confirm or refute the DAMA result during the following full-scale experimental phase. The impact of background radiation from the detector materials and the experimental site needs to be carefully investigated, including both intrinsic and cosmogenically activated radioactivity. Based on the best knowledge of the most relevant sources of background, we have performed a detailed Monte Carlo study evaluating the expected background in the dark matter search spectral region. The simulation model described in this paper guides the design of the full-scale experiment and will be fundamental for the interpretation of the measured background and hence for the extraction of a possible dark matter signal

Signatures of muonic activation in the Majorana Demonstrator

Experiments searching for very rare processes such as neutrinoless double-beta decay require a detailed understanding of all sources of background. Signals from radioactive impurities present in construction and detector materials can be suppressed using a number of well-understood techniques. Background from in situ cosmogenic interactions can be reduced by siting an experiment deep underground. However, the next generation of such experiments have unprecedented sensitivity goals of 1028 years half-life with background rates of 10-5cts/(keV kg yr) in the region of interest. To achieve these goals, the remaining cosmogenic background must be well understood. In the work presented here, Majorana Demonstrator data are used to search for decay signatures of metastable germanium isotopes. Contributions to the region of interest in energy and time are estimated using simulations and compared to Demonstrator data. Correlated time-delayed signals are used to identify decay signatures of isotopes produced in the germanium detectors. A good agreement between expected and measured rate is found and different simulation frameworks are used to estimate the uncertainties of the predictions. The simulation campaign is then extended to characterize the background for the LEGEND experiment, a proposed tonne-scale effort searching for neutrinoless double-beta decay in Ge76

Experimental study of C 13 (α,n) O 16 reactions in the Majorana Demonstrator calibration data

Neutron captures and delayed decays of reaction products are common sources of backgrounds in ultrarare event searches. In this work, we studied C13(α,n)O16 reactions induced by α particles emitted within the calibration sources of the Majorana Demonstrator. These sources are thorium-based calibration standards enclosed in carbon-rich materials. The reaction rate was estimated by using the 6129-keV γ rays emitted from the excited O16 states that are populated when the incoming α particles exceed the reaction Q value. Thanks to the excellent energy performance of the Demonstrator's germanium detectors, these characteristic photons can be clearly observed in the calibration data. Facilitated by Geant4 simulations, a comparison between the observed 6129-keV photon rates and predictions by a talys-based software was performed. The measurements and predictions were found to be consistent, albeit with large statistical uncertainties. This agreement provides support for background projections from (α,n) reactions in future double-beta decay search efforts

The Majorana Demonstrator: A Search for Neutrinoless Double-beta Decay of 76Ge

Neutrinoless double-beta (0νββ) decay is a hypothesized process where in some even-even nuclei it might be possible for two neutrons to simultaneously decay into two protons and two electrons without emitting neutrinos. This is possible only if neutrinos are Majorana particles, i.e. fermions that are their own antiparticles. Neutrinos being Majorana particles would explicitly violate lepton number conservation, and might play a role in the matter-antimatter asymmetry in the universe. The observation of neutrinoless double-beta decay would also provide complementary information related to neutrino masses. The Majorana Collaboration is constructing the MAJORANA DEMONSTRATOR, with a total of 40-kg Germanium detectors, to search for the 0νββ decay of 76Ge and to demonstrate a background rate at or below 3 counts/(ROI•t•y) in the 4 keV region of interest (ROI) around the 2039 keV Q-value for 76Ge 0νββ decay. In this paper, we discuss the physics of neutrinoless double beta decay and then focus on the MAJORANA DEMONSTRATOR, including its design and approach to achieve ultra-low backgrounds and the status of the experiment

The Majorana Demonstrator readout electronics system

The Majorana Demonstrator comprises two arrays of high-purity germanium detectors constructed to search for neutrinoless double-beta decay in 76Ge and other physics beyond the Standard Model. Its readout electronics were designed to have low electronic noise, and radioactive backgrounds were minimized by using low-mass components and low-radioactivity materials near the detectors. This paper provides a description of all components of the Majorana Demonstrator readout electronics, spanning the front-end electronics and internal cabling, back-end electronics, digitizer, and power supplies, along with the grounding scheme. The spectroscopic performance achieved with these readout electronics is also demonstrated