Metabolic Responses to Carbohydrate Ingestion during Exercise: Associations between Carbohydrate Dose and Endurance Performance

Carbohydrate (CHO) ingestion during exercise lasting less than three hours improves endurance exercise performance but there is still debate about the optimal dose. We utilised stable isotopes and blood metabolite profiles to further examine metabolic responses to CHO (glucose only) ingestion in the 20–64 g·h−1 range, and to determine the association with performance outcome. In a double-blind, randomized cross-over design, male cyclists (n = 20, mean ± SD, age 34 ± 10 years, mass 75.8 ± 9 kg, peak power output 394 ± 36 W, VO2max 62 ± 9 mL·kg−1·min−1) completed four main experimental trials. Each trial involved a two-hour constant load ride (185 ± 25 W) followed by a time trial, where one of three CHO beverages, or a control (water), were administered every 15 min, providing 0, 20, 39 or 64 g CHO·h−1. Dual glucose tracer techniques, indirect calorimetry and blood analyses were used to determine glucose kinetics, exogenous CHO oxidation (EXO), endogenous CHO and fat oxidation; and metabolite responses. Regression analysis revealed that total exogenous CHO oxidised in the second hour of exercise, and suppression of serum NEFA concentration provided the best prediction model of performance outcome. However, the model could only explain ~19% of the variance in performance outcome. The present data demonstrate that consuming ~40 g·h−1 of CHO appears to be the minimum ingestion rate required to induce metabolic effects that are sufficient to impact upon performance outcome. These data highlight a lack of performance benefit and few changes in metabolic outcomes beyond an ingestion rate of 39 g·h−1. Further work is required to explore dose-response effects of CHO feeding and associations between multiple metabolic parameters and subsequent performance outcome

Extension to order β23\beta^{23} of the high-temperature expansions for the spin-1/2 Ising model on the simple-cubic and the body-centered-cubic lattices

Using a renormalized linked-cluster-expansion method, we have extended to order $\beta^{23}$ the high-temperature series for the susceptibility $\chi$ and the second-moment correlation length $\xi$ of the spin-1/2 Ising models on the sc and the bcc lattices. A study of these expansions yields updated direct estimates of universal parameters, such as exponents and amplitude ratios, which characterize the critical behavior of $\chi$ and $\xi$. Our best estimates for the inverse critical temperatures are $\beta^{sc}_c=0.221654(1)$ and $\beta^{bcc}_c=0.1573725(6)$. For the susceptibility exponent we get $\gamma=1.2375(6)$ and for the correlation length exponent we get $\nu=0.6302(4)$. The ratio of the critical amplitudes of $\chi$ above and below the critical temperature is estimated to be $C_+/C_-=4.762(8)$. The analogous ratio for $\xi$ is estimated to be $f_+/f_-=1.963(8)$. For the correction-to-scaling amplitude ratio we obtain $a^+_{\xi}/a^+_{\chi}=0.87(6)$.Comment: Misprints corrected, 8 pages, latex, no figure

Critical exponents and equation of state of the three-dimensional Heisenberg universality class

We improve the theoretical estimates of the critical exponents for the three-dimensional Heisenberg universality class. We find gamma=1.3960(9), nu=0.7112(5), eta=0.0375(5), alpha=-0.1336(15), beta=0.3689(3), and delta=4.783(3). We consider an improved lattice phi^4 Hamiltonian with suppressed leading scaling corrections. Our results are obtained by combining Monte Carlo simulations based on finite-size scaling methods and high-temperature expansions. The critical exponents are computed from high-temperature expansions specialized to the phi^4 improved model. By the same technique we determine the coefficients of the small-magnetization expansion of the equation of state. This expansion is extended analytically by means of approximate parametric representations, obtaining the equation of state in the whole critical region. We also determine a number of universal amplitude ratios.Comment: 40 pages, final version. In publication in Phys. Rev.

(Sub)mm Interferometry Applications in Star Formation Research

This contribution gives an overview about various applications of (sub)mm interferometry in star formation research. The topics covered are molecular outflows, accretion disks, fragmentation and chemical properties of low- and high-mass star-forming regions. A short outlook on the capabilities of ALMA is given as well.Comment: 20 pages, 7 figures, in proceedings to "2nd European School on Jets from Young Star: High Angular Resolution Observations". A high-resolution version of the paper can be found at http://www.mpia.de/homes/beuther/papers.htm

Twisted carotenoids do not support efficient intramolecular singlet fission in the orange carotenoid protein

Singlet exciton fission is the spin-allowed generation of two triplet electronic excited states from a singlet state. Intramolecular singlet fission has been suggested to occur on individual carotenoid molecules within protein complexes provided that the conjugated backbone is twisted out of plane. However, this hypothesis has been forwarded only in protein complexes containing multiple carotenoids and bacteriochlorophylls in close contact. To test the hypothesis on twisted carotenoids in a “minimal” one-carotenoid system, we study the orange carotenoid protein (OCP). OCP exists in two forms: in its orange form (OCPo), the single bound carotenoid is twisted, whereas in its red form (OCPr), the carotenoid is planar. To enable room-temperature spectroscopy on canthaxanthin-binding OCPo and OCPr without laser-induced photoconversion, we trap them in a trehalose glass. Using transient absorption spectroscopy, we show that there is no evidence of long-lived triplet generation through intramolecular singlet fission despite the canthaxanthin twist in OCPo

Variation of the IMF

(abridged) The {stellar IMF} has been found to be essentially invariant. While some apparent differences are seen, the uncertainties inherent to this game do not allow a firm conclusion to be made that the IMF varies systematically with conditions. The IMF integrated over entire galaxies, however, is another matter. Chemical and photometric properties of various galaxies do hint at {galaxial IMFs} being steeper than the stellar IMF, as is also deduced from direct star-count analysis in the MW. These results are sensitive to the modelling of stellar populations and to corrections for stellar evolution, and are thus also uncertain. However, by realising that galaxies are made from dissolving star clusters, star clusters being viewed as {the fundamental building blocks of galaxies}, the result is found that galaxial IMFs must be significantly steeper than the stellar IMF, because the former results from a folding of the latter with the star-cluster mass function. Furthermore, this notion leads to the important insight that galaxial IMFs must vary with galaxy mass, and that the galaxial IMF is a strongly varying function of the star-formation history for galaxies that have assembled only a small mass in stars. Cosmological implications of this are discussed.Comment: 13 pages, to appear in IMFat50: The Initial Mass Function 50 years later, ed: E. Corbelli, F. Palla, and H. Zinnecker, Kluwer Academic Publishers; a meeting held at the Abbazia di Spineto, Tuscany, Italy -- May 16-20, 200

Loss of Grem1-lineage chondrogenic progenitor cells causes osteoarthritis

Published online: 31 October 2023Osteoarthritis (OA) is characterised by an irreversible degeneration of articular cartilage. Here we show that the BMP-antagonist Gremlin 1 (Grem1) marks a bipotent chondrogenic and osteogenic progenitor cell population within the articular surface. Notably, these progenitors are depleted by injury-induced OA and increasing age. OA is also caused by ablation of Grem1 cells in mice. Transcriptomic and functional analysis in mice found that articular surface Grem1-lineage cells are dependent on Foxo1 and ablation of Foxo1 in Grem1-lineage cells caused OA. FGFR3 signalling was confirmed as a promising therapeutic pathway by administration of pathway activator, FGF18, resulting in Grem1-lineage chondrocyte progenitor cell proliferation, increased cartilage thickness and reduced OA. These findings suggest that OA, in part, is caused by mechanical, developmental or age-related attrition of Grem1 expressing articular cartilage progenitor cells. These cells, and the FGFR3 signalling pathway that sustains them, may be effective future targets for biological management of OA.Jia Q. Ng, Toghrul H. Jafarov, Christopher B. Little, Tongtong Wang, Abdullah M. Ali, YanMa, Georgette A. Radford, Laura Vrbanac, Mari Ichinose, Samuel Whittle, David J. Hunter, Tamsin R. M. Lannagan, Nobumi Suzuki, JarradM. Goyne, Hiroki Kobayashi, Timothy C. Wang, David R. Haynes, Danijela Menicanin, Stan Gronthos, Daniel L. Worthley, Susan L. Woods and Siddhartha Mukherje

Generalized biomolecular modeling and design with RoseTTAFold All-Atom

Deep learning methods have revolutionized protein structure prediction and design but are currently limited to protein-only systems. We describe RoseTTAFold All-Atom (RFAA) which combines a residue-based representation of amino acids and DNA bases with an atomic representation of all other groups to model assemblies containing proteins, nucleic acids, small molecules, metals, and covalent modifications given their sequences and chemical structures. By fine tuning on denoising tasks we obtain RFdiffusionAA, which builds protein structures around small molecules. Starting from random distributions of amino acid residues surrounding target small molecules, we design and experimentally validate, through crystallography and binding measurements, proteins that bind the cardiac disease therapeutic digoxigenin, the enzymatic cofactor heme, and the light harvesting molecule bilin