MENGA: a new comprehensive tool for the integration of neuroimaging data and the Allen human brain transcriptome atlas

Brain-wide mRNA mappings offer a great potential for neuroscience research as they can provide information about system proteomics. In a previous work we have correlated mRNA maps with the binding patterns of radioligands targeting specific molecular systems and imaged with positron emission tomography (PET) in unrelated control groups. This approach is potentially applicable to any imaging modality as long as an efficient procedure of imaging-genomic matching is provided. In the original work we considered mRNA brain maps of the whole human genome derived from the Allen human brain database (ABA) and we performed the analysis with a specific region-based segmentation with a resolution that was limited by the PET data parcellation. There we identified the need for a platform for imaging-genomic integration that should be usable with any imaging modalities and fully exploit the high resolution mapping of ABA dataset.In this work we present MENGA (Multimodal Environment for Neuroimaging and Genomic Analysis), a software platform that allows the investigation of the correlation patterns between neuroimaging data of any sort (both functional and structural) with mRNA gene expression profiles derived from the ABA database at high resolution.We applied MENGA to six different imaging datasets from three modalities (PET, single photon emission tomography and magnetic resonance imaging) targeting the dopamine and serotonin receptor systems and the myelin molecular structure. We further investigated imaging-genomic correlations in the case of mismatch between selected proteins and imaging targets

Defining the dialogue between sciences: A view on transdisciplinary perspective in the human sciences

The authors argue that interdisciplinarity, together with the more recent concept of transdisciplinarity, can be seen as a coherent attempt not so much to reas-semble the fragmented structure into a whole, as to create a fruitful collaboration and integration among different disciplines that takes into account their specificity. Background At the threshold of the Modern Age, a series of paradigm shifts in Western thought caused its fragmentation into a variety of academic subdisciplines. Such diversification can be considered the result of epistemological shifts and changes in the division of intellectual labor. Contribution Which semantic horizons can this new approach open, and on which theoretical foundations could a dialogue between disciplines be produced? The growing importance of this problem is evidenced by the emergence, during the last decades, of philosophical reflections on the interactions among different research fields. Findings The possibility of transdisciplinarity in modern science finds its theoretical premise in M. Foucault's seminal work on the organization of knowledge, The Order of Things, which hinted at the existence of gaps in the grid of knowledge, leading, as a result, to the possibility of creating transdisciplinary connections. Impact on Society The paper aims to contribute to the contemporary discussion of the need to overcome boundaries between disciplines. Consequently, it has both a methodo logical and theoretical impact, since all branches of knowledge aspiring to go beyond their traditional theoretical boundaries would benefit from a coherent theoretical perspective which tries to reconceptualize the transfer of knowledge from one field to another. Future Research The authors' critical discussion of transdisciplinarity aims to revive the French epistemological tradition that in the last decades has often been rejected by researchers as not being rigorous nor analytical. This choice is motivated by the belief that, despite such evident defects, at its bottom lies a genuine theoretical intention that does not take for granted the possibility of transcending the usual division of intellectual work. In addition, the authors offer a brief account of the Russian conception of transdisciplinarity, relatively little studied in the West, which is presumed to integrate and solve the difficulties of other similar mod-els

Multi-sensor analysis of extreme events in North-Eastern Italy

International audienceThe North-eastern part of Italy is known to be one of the most rainy regions in Europe. In this paper three extreme events are analysed, using a multi-sensor observing system including a weather radar and a dense telemetric network of surface stations, recording precipitation, wind, temperature and relative humidity. The cases examined comprise two long lasting rainfall events impacting two distinct areas, and a vigorous hail-producing thunderstorm event over the plains. In all cases, inter-comparison between remotely sensed and surface observations, including estimates and measures of precipitation and wind, helps to better understand the behaviour of the atmosphere, thus supporting operational fore- and now-casting. In the case of widespread precipitation, a relation is suggested between the wind speed and direction at medium/low levels with the location of the maximum precipitation relative to the mountains. This reflects the dynamical interaction between the mountain barrier and the atmospheric flux impinging upon it. This flux can be estimated by the automatic weather station of Mt. Cesen, a focal point for a now-casting of the rain in the Veneto Region. Analysis of strong thunderstorm activity makes extensive use of radar data. In the examined case the interaction of a sea breeze-like circulation with a mesoscale trough gave rise to a distinct convergence line that triggered a severe and long-lived hail-producing multi-cell thunderstorm. The hail was successfully detected by the radar's hail detection algorithm

Prevention of vertical transmission of hepatitis B virus infection

Hepatitis B virus (HBV) is the leading cause of chronic viral hepatitis. Annually, almost two million children younger than 5 years acquire the infection, mostly through vertical or horizontal transmission in early life. Vertical transmission of HBV is a high efficacy phenomenon ranging, in the absence of any preventive interventions, from 70% to 90% for hepatitis e antigen positive mothers and from 10% to 40% for hepatitis e antigen-negative mothers. Maternal viraemia is a preeminent risk factor for vertical transmission of HBV. Maternal screening is the first step to prevent vertical transmission of HBV. Hepatitis B passive and active immunoprophylaxis at birth together with antiviral treatment of highly viraemic mothers are the key strategies for global elimination of HBV infection. Strategies are needed to promote implementation of birth-dose vaccination and hepatitis B immunoglobulins in low- and middle-income countries where the prevalence of the infection is at the highest

Treatment of multidrug-resistant and extensively drug-resistant tuberculosis in children: The role of bedaquiline and delamanid

Multidrug-resistant (MDR) tuberculosis (TB) has been emerging at an alarming rate over the last few years. It has been estimated that about 3% of all pediatric TB is MDR, meaning about 30,000 cases each year. Although most children with MDR-TB can be successfully treated, up to five years ago effective treatment was associated with a high incidence of severe adverse effects and patients with extensively drug-resistant (XDR) TB had limited treatment options and no standard regimen. The main objective of this manuscript is to discuss our present knowledge of the management of MDR-and XDR-TB in children, focusing on the characteristics and available evidence on the use of two promising new drugs: bedaquiline and delamanid. PubMed was used to search for all of the studies published up to November 2020 using key words such as “bedaquiline” and “delamanid” and “children” and “multidrug-resistant tuberculosis” and “extensively drug-resistant tuberculosis”. The search was limited to articles published in English and providing evidence-based data. Although data on pediatric population are limited and more studies are needed to confirm the efficacy and safety of bedaquiline and delamanid, their use in children with MDR-TB/XDR-TB appears to have good tolerability and efficacy. However, more evidence on these new anti-TB drugs is needed to better guide their use in children in order to design effective shorter regimens and reduce adverse effects, drug interactions, and therapeutic failure

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Hoffa’s fat pad thickness: a measurement method with sagittal MRI sequences

Background: Hoffa’s fat pad is a structure located within the fibrous joint capsule of the knee joint, but outside the synovial cavity. It plays an important biomechanical and metabolic role in knee joint, reducing the impact of forces generated by loading and producing cytokines. Changes in its size can induce modifications in the knee homeostasis. However, a great variability exists regarding its measurements. This work aims to evaluate the reliability of a measurement method of Hoffa’s fat pad dimensions through MRI. Methods: 3T sagittal IW 2D TSE fat-suppressed MRI sequences, taken from the OAI (Osteoarthritis initiative) database, of 191 male and female patients, aged between 40 and 80 years, were analysed; a manual measurement of the thickness of Hoffa’s fat pad of each subject was then performed by two different readers. The interobserver reliability and intraobserver reliability of the measurements were described by coefficient of variation (CV), Pearson correlation and Bland–Altman plots. Results: All statistical analyses have shown that not significant intra- or interobservers differences were evident (intraobserver CV % for the first observer was 2.17% for the right knee and 2.24% for the left knee, while for the second observer 2.31% for the right knee and 2.24% for the left knee; linear correlation was for the first observer r = 0.96 for the right knee and r = 0.96 for the left knee, while for the second observer r = 0.97 for the right knee and r = 0.96 for the left knee; in addition, the interobserver CV % was 1.25% for the right knee and 1.21% for the left knee and a high interobserver linear correlation was found: r = 0.97 for the right knee and r = 0.96 for the left knee). All results suggest that this manual measurement method of Hoffa’s fat pad thickness can be performed with satisfactory intra- and interobserver reliability. Conclusions: Hoffa’s fat pad thickness can be measured, using sagittal MRI images, with this manual method that represents, for his high reliability, an effective means for the study of this anatomical structure

Efficacy of topical imiquimod 3.75% in the treatment of actinic keratosis of the scalp in immunosuppressed patients: our case series

AbstractBackground: Actinic keratoses (AK) represent common cutaneous lesions, appearing in 'Field cancerization areas' and potentially evolving toward invasive neoplasm. Immunosuppressed patients ..