514 research outputs found
Serodiagnosis of infectious mononucleosis by using recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology
Four recombinant, diagnostically useful Epstein-Barr virus (EBV) proteins representative of the viral capsid antigen (p150), diffuse early antigen (p54), the major DNA-binding protein (p138), and the EBV nuclear antigen (p72) (W. Hinderer, H. Nebel-Schickel, H.H. Sonneborn, M. Motz, R. Kühbeck, and H. Wolf, J. Exp. Clin. Cancer Res. 7[Suppl.]:132, 1988) were used to set up individual enzyme-linked immunosorbent assays (ELISAs) for the qualitative and quantitative detection of immunoglobulin M (IgM) and IgG antibodies. In direct comparison with results obtained by standard immunofluorescence or immunoperoxidase assays, it was then shown that the recombinant EBV ELISAs provide the means for specific and sensitive serodiagnosis of infectious mononucleosis (IM) caused by EBV. The most useful markers in sera from such patients proved to be IgM antibodies against p54, p138, and p150. Additional positive markers for recent or ongoing IM apparently were IgG antibodies against p54 and p138. In contrast, anti-p72 IgG had a high preference for sera from healthy blood donors and, therefore, can be considered indicative of past exposure to the virus. Altogether, the individual ELISAs proved to be as specific and at least as sensitive for the diagnosis of IM as the currently available standard techniques are. Moreover, our findings suggest that, by combining individual test antigens, a workable ELISA system consisting of three assays (IgM against p54, p138, and p150; IgG against p54 and p138; and IgG against p72) can be established for the standardized rapid diagnosis of acute EBV infections
The Pauli equation with complex boundary conditions
We consider one-dimensional Pauli Hamiltonians in a bounded interval with
possibly non-self-adjoint Robin-type boundary conditions. We study the
influence of the spin-magnetic interaction on the interplay between the type of
boundary conditions and the spectrum. A special attention is paid to
PT-symmetric boundary conditions with the physical choice of the time-reversal
operator T.Comment: 16 pages, 4 figure
Implementing concurrent-training and nutritional strategies in professional football: a complex challenge for coaches and practitioners
Purpose: To study concurrent-training (CT) and nutritional practices within a professional soccer team. Methods: Twenty-one professional football players competing in the English professional league participated in this study (mean ± standard deviations [M ± SD] 26 ± 4 years, stature 1.84 ± 0.1 m, body mass 83 ± 7 kg, VO2max; 58 ± 3 ml · kg−1 · min−1). A range of internal and external training metrics, the organisation of CT (training sequence, training rest period between bouts) and the nutritional intake around CT (timing, type and quantity) was collected for 10 weeks. Results: CT; n = 17 (endurance-training [ET] + resistance-training [RT]; n = 11; RT + ET; n = 6) rest period between bouts was not consistent and varied depending on the sequence of CT (RT + ET, 75 ± 48 min; ET + RT; 60 ± 5 min; P = 0.04). sRPE of football-specific ET was higher in RT + ET (RT + ET, 7 ± 1; ET + RT, 6 ± 1; P = 0.05). The timing of meals around training was influenced by the organisation of CT. Subsequently, CHO consumption before training session one was significantly less in RT + ET (CHO 0.10 ± 0.5 g · kg−1 vs. CHO 0.45 ± 0.2 g · kg−1). Conclusion: The present data demonstrate that the organisation of CT (i.e., exercise order and/or recovery time between bouts) and nutrition (i.e., timing of meal intake) can be unsystematic in the applied environment. The organisation of training and nutrition might influence the players’ ability to perform high-intensity actions in secondary training sessions and could potentially impact acute metabolic processes associated with muscle recovery and muscle adaptation
Scattering in the PT-symmetric Coulomb potential
Scattering on the -symmetric Coulomb potential is studied along a
U-shaped trajectory circumventing the origin in the complex plane from
below. This trajectory reflects symmetry, sets the appropriate
boundary conditions for bound states and also allows the restoration of the
correct sign of the energy eigenvalues. Scattering states are composed from the
two linearly independent solutions valid for non-integer values of the 2L
parameter, which would correspond to the angular momentum in the usual
Hermitian setting. Transmission and reflection coefficients are written in
closed analytic form and it is shown that similarly to other -symmetric scattering systems the latter exhibit handedness effect.
Bound-state energies are recovered from the poles of the transmission
coefficients.Comment: Journal of Physics A: Mathematical and Theoretical 42 (2009) to
appea
Pseudospectra in non-Hermitian quantum mechanics
We propose giving the mathematical concept of the pseudospectrum a central
role in quantum mechanics with non-Hermitian operators. We relate
pseudospectral properties to quasi-Hermiticity, similarity to self-adjoint
operators, and basis properties of eigenfunctions. The abstract results are
illustrated by unexpected wild properties of operators familiar from
PT-symmetric quantum mechanics.Comment: version accepted for publication in J. Math. Phys.: criterion
excluding basis property (Proposition 6) added, unbounded time-evolution
discussed, new reference
Beta-delayed-neutron studies of Sb and I performed with trapped ions
Beta-delayed-neutron (n) spectroscopy was performed using the
Beta-decay Paul Trap and an array of radiation detectors. The n
branching ratios and energy spectra for Sb and I were
obtained by measuring the time of flight of recoil ions emerging from the
trapped ion cloud. These nuclei are located at the edge of an isotopic region
identified as having n branching ratios that impact the r-process
abundance pattern around the A~130 peak. For Sb and I,
n branching ratios of 14.6(11)%, 17.6(28)%, and 7.6(28)% were
determined, respectively. The n energy spectra obtained for Sb
and I are compared with results from direct neutron measurements, and
the n energy spectrum for Sb has been measured for the first
time
Non linear pseudo-bosons versus hidden Hermiticity. II: The case of unbounded operators
Parallels between the notions of nonlinear pseudobosons and of an apparent
non-Hermiticity of observables as shown in paper I (arXiv: 1109.0605) are
demonstrated to survive the transition to the quantum models based on the use
of unbounded metric in the Hilbert space of states.Comment: 21 p
- …