47 research outputs found

    A Role for Strain Differences in Waveforms of Ultrasonic Vocalizations during Male–Female Interaction

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    Male mice emit ultrasonic vocalizations (USVs) towards females during male–female interaction. It has been reported that USVs of adult male mice have the capability of attracting females. Although the waveform pattern of USVs is affected by genetic background, differences among strains with respect to USV and the effects of these differences on courtship behavior have not been analyzed fully. We analyzed USV patterns, as well as actual social behavior during USV recording, in 13 inbred mouse strains, which included laboratory and wild-derived strains. Significant effects of strain were observed for the frequency of USV emission, duration, and frequency of the waveform category. Principal component (PC) analysis showed that PC1 was related to frequency and duration, and PC2–4 were related to each waveform. In the comparison of USV patterns and behaviors among strains, wild-derived KJR mice displayed the highest scores for PC2–4, and female mice paired with KJR males did not emit rejection-related click sounds. It is assumed that the waveforms emitted by KJR males have a positive effect in male–female interaction. Therefore, we extracted waveforms in PC2–4 from the USV recordings of KJR mice to produce a sound file, "HIGH2-4". As a negative control, another sound file ("LOW2-4") was created by extracting waveforms in PC2-4 from strains with low scores for these components. In the playback experiments using these sound files, female mice were attracted to the speaker that played HIGH2-4 but not the speaker that played LOW2-4. These results highlight the role of strain differences in the waveforms of male USVs during male–female interaction. The results indicated that female mice use male USVs as information when selecting a suitable mate

    Epstein-Barr Virus LMP2A Reduces Hyperactivation Induced by LMP1 to Restore Normal B Cell Phenotype in Transgenic Mice

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    Epstein-Barr virus (EBV) latently infects most of the human population and is strongly associated with lymphoproliferative disorders. EBV encodes several latency proteins affecting B cell proliferation and survival, including latent membrane protein 2A (LMP2A) and the EBV oncoprotein LMP1. LMP1 and LMP2A signaling mimics CD40 and BCR signaling, respectively, and has been proposed to alter B cell functions including the ability of latently-infected B cells to access and transit the germinal center. In addition, several studies suggested a role for LMP2A modulation of LMP1 signaling in cell lines by alteration of TRAFs, signaling molecules used by LMP1. In this study, we investigated whether LMP1 and LMP2A co-expression in a transgenic mouse model alters B cell maturation and the response to antigen, and whether LMP2A modulates LMP1 function. Naïve LMP1/2A mice had similar lymphocyte populations and antibody production by flow cytometry and ELISA compared to controls. In the response to antigen, LMP2A expression in LMP1/2A animals rescued the impairment in germinal center generation promoted by LMP1. LMP1/2A animals produced high-affinity, class-switched antibody and plasma cells at levels similar to controls. In vitro, LMP1 upregulated activation markers and promoted B cell hyperproliferation, and co-expression of LMP2A restored a wild-type phenotype. By RT-PCR and immunoblot, LMP1 B cells demonstrated TRAF2 levels four-fold higher than non-transgenic controls, and co-expression of LMP2A restored TRAF2 levels to wild-type levels. No difference in TRAF3 levels was detected. While modulation of other TRAF family members remains to be assessed, normalization of the LMP1-induced B cell phenotype through LMP2A modulation of TRAF2 may be a pathway by which LMP2A controls B cell function. These findings identify an advance in the understanding of how Epstein-Barr virus can access the germinal center in vivo, a site critical for both the genesis of immunological memory and of virus-associated tumors

    Hidden Markov models and mouse ultrasonic vocalizations

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    (a) B6 mice differentially approached socially conditioned cues relative to novel cues (*orthogonal contrast for social approach vs

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    <p><b>Copyright information:</b></p><p>Taken from "Social reward among juvenile mice"</p><p></p><p>Genes, Brain, and Behavior 2007;6(7):661-671.</p><p>Published online Jan 2007</p><p>PMCID:PMC2040181.</p><p>© 2006 The Authors Journal compilation</p> novelty approach, = 0.03, social approach = time in social environment time in novel environment). (b) Unconditioned B6 mice did not respond differentially to the presentation of novel cues in the testing arena (novelty approach = time in novel environment time in familiar environment). (c) B6 mice approached novel environments only when the other peripheral compartment of the testing arena contained cues that predicted social isolation (orthogonal contrast for isolation aversion vs. novelty approach, < 0.001, isolation aversion = time in novel environment time in isolate environment). Data in each panel are presented as the mean ± SEM

    (a–c) Frequency distributions illustrate the number of mice (ordinate) expressing a particular preference score (abscissa) following (a) no conditioning, (b) social conditioning or (c) food conditioning

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    <p><b>Copyright information:</b></p><p>Taken from "Social reward among juvenile mice"</p><p></p><p>Genes, Brain, and Behavior 2007;6(7):661-671.</p><p>Published online Jan 2007</p><p>PMCID:PMC2040181.</p><p>© 2006 The Authors Journal compilation</p> Mice from all of the strains were included in the distributions. (a′–c′) Juvenile mice did not differentially approach or explore the environments (a′) without conditioning. (b′) Social conditioning resulted in a CPP for mice from three strains but not BALB mice (Tukey’s HSD tests, * < 0.05 for all tests comparing BALB with the other strains). (c′) Mice from all of the strains learned the conditioning contingency when food was used as a reward. Preference scores were calculated as the duration spent in the reward-paired (social or food) environment the duration spent in the reward-impoverished (isolation or food deprivation) environment. (a″–c″) There were strain-dependent differences in locomotor activity (a″) without conditioning, (b″) with social conditioning and (c″) with food conditioning. There was no difference in exploratory activity between BALB and B6 mice that were tested following the food conditioning procedure (Tukey’s HSD tests, * < 0.05 compared with all other strains, < 0.05 compared with the A strain, < 0.05 compared with the A and DBA strains). Data in panels a′–c′ and a″–c″ are presented as the mean ± SEM

    Lack of Visual Orienting to Biological Motion and Audiovisual Synchrony in 3-Year-Olds with Autism

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    It has been suggested that children with autism orient towards audiovisual synchrony (AVS) rather than biological motion and that the opposite pattern is to be expected in typical development. Here, we challenge this notion by showing that 3-year-old neurotypical children orient to AVS and to biological motion in point-light displays but that 3-year-old children with autism orient to neither of these types of information. Thus, our data suggest that two fundamental mechanisms are disrupted in young children with autism: one that supports orienting towards others' movements and one that supports orienting towards multimodally specified events. These impairments may have consequences for socio-cognitive development and brain organization
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