Effects of Aging and Cyclosporin A on Collagen Turnover in Human Gingiva

BACKGROUND: WE AIMED AT CHARACTERIZING THE AGING GINGIVA ANALYZING: i) collagen content and turnover in human gingival tissues and fibroblasts obtained from healthy young and aging subjects. ii) the effect of cyclosporin A administration in human cultured gingival fibroblasts obtained from aging compared to young subjects. METHODS: Morphological analysis was performed on haematoxylin-eosin and Sirius red stained paraffin-embedded gingival biopsies from young and aging healthy subjects. The expression of the main genes and proteins involved in collagen turnover were determined by real time PCR, dot blot and SDS-zymography on cultured young and aging gingival fibroblasts, and after cyclosporin A administration. RESULTS: Our results suggest that in healthy aged people, gingival connective tissue is characterized by a similar collagen content and turnover. Collagen turnover pathways are similarly affected by cyclosporin A treatment in young and aging gingival fibroblasts. CONCLUSIONS: Cyclosporin A administration affects gingival collagen turnover pathways in young and aging fibroblasts at the same extent, suggesting that during aging cyclosporin A administration is not related to relevant collagen turnover modifications

Meta-Profiles of Gene Expression during Aging: Limited Similarities between Mouse and Human and an Unexpectedly Decreased Inflammatory Signature

Background: Skin aging is associated with intrinsic processes that compromise the structure of the extracellular matrix while promoting loss of functional and regenerative capacity. These processes are accompanied by a large-scale shift in gene expression, but underlying mechanisms are not understood and conservation of these mechanisms between humans and mice is uncertain. Results: We used genome-wide expression profiling to investigate the aging skin transcriptome. In humans, age-related shifts in gene expression were sex-specific. In females, aging increased expression of transcripts associated with T-cells, B-cells and dendritic cells, and decreased expression of genes in regions with elevated Zeb1, AP-2 and YY1 motif density. In males, however, these effects were contrasting or absent. When age-associated gene expression patterns in human skin were compared to those in tail skin from CB6F1 mice, overall human-mouse correspondence was weak. Moreover, inflammatory gene expression patterns were not induced with aging of mouse tail skin, and well-known aging biomarkers were in fact decreased (e.g., Clec7a, Lyz1 and Lyz2). These unexpected patterns and weak human-mouse correspondence may be due to decreased abundance of antigen presenting cells in mouse tail skin with age. Conclusions: Aging is generally associated with a pro-inflammatory state, but we have identified an exception to this pattern with aging of CB6F1 mouse tail skin. Aging therefore does not uniformly heighten inflammatory status across all mouse tissues. Furthermore, we identified both intercellular and intracellular mechanisms of transcriptome aging, including those that are sex- and species-specific

Effects of a vegetable extract from Lupinus albus (LU105) on the production of matrix metalloproteinases (MMP1, MMP2, MMP9) and tissue inhibitor of metalloproteinases (TIMP1, TIMP2) by human gingival fibroblasts in culture

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Protective effect of a vegetable extract from Lupinus albus (LU 105) on human gingival elastic fibers degradation by human leukocyte elastase

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Thermal ion measurements on board Interball Auroral Probe by the Hyperboloid experiment

Hyperboloid is a multi-directional mass spectrometer measuring ion distribution functions in the auroral and polar magnetosphere of the Earth in the thermal and suprathermal energy range. The instrument encompasses two analyzers containing a total of 26 entrance windows, and viewing in two almost mutually perpendicular half-planes. The nominal angular resolution is defined by the field of view of individual windows ≈13° × 12.5°. Energy analysis is performed using spherical electrostatic analyzers providing differential measurements between 1 and 80 eV. An ion beam emitter (RON experiment) and/or a potential bias applied to Hyperboloid entrance surface are used to counteract adverse effects of spacecraft potential and thus enable ion measurements down to very low energies. A magnetic analyzer focuses ions on one of four micro-channel plate (MCP) detectors, depending on their mass/charge ratio. Normal modes of operation enable to measure H+, He+, O++, and O+ simultaneously. An automatic MCP gain control software is used to adapt the instrument to the great flux dynamics encountered between spacecraft perigee (700 km) and apogee (20 000 km). Distribution functions in the main analyzer half-plane are obtained after a complete scan of windows and energies with temporal resolution between one and a few seconds. Three-dimensional (3D) distributions are measured in one spacecraft spin period (120 s). The secondary analyzer has a much smaller geometrical factor, but offers partial access to the 3D dependence of the distributions with a few seconds temporal resolution. Preliminary results are presented. Simultaneous, local heating of both H+ and O+ ions resulting in conical distributions below 80 eV is observed up to 3 Earth's radii altitudes. The thermal ion signatures associated with large-scale nightside magnetospheric boundaries are investigated and a new ion outflow feature is identified associated to the polar edge of the auroral oval. Detailed distribution functions of injected magnetosheath ions and ouflowing cleft fountain ions are measured down to a few eVs in the dayside.Key words. Ionosphere (auroral ionosphere; particle acceleration; ionosphere-magnetosphere interactions) &nbsp

Thermal ion measurements on board Interball Auroral Probe by the Hyperboloid experiment

International audienceHyperboloid is a multi-directional mass spectrometer measuring ion distribution functions in the auroral and polar magnetosphere of the Earth in the thermal and suprathermal energy range. The instrument encompasses two analyzers containing a total of 26 entrance windows, and viewing in two almost mutually perpendicular half-planes. The nominal angular resolution is defined by the field of view of individual windows ˜13° × 12.5°. Energy analysis is performed using spherical electrostatic analyzers providing differential measurements between 1 and 80 eV. An ion beam emitter (RON experiment) and/or a potential bias applied to Hyperboloid entrance surface are used to counteract adverse effects of spacecraft potential and thus enable ion measurements down to very low energies. A magnetic analyzer focuses ions on one of four micro-channel plate (MCP) detectors, depending on their mass/charge ratio. Normal modes of operation enable to measure H+, He+, O++, and O+ simultaneously. An automatic MCP gain control software is used to adapt the instrument to the great flux dynamics encountered between spacecraft perigee (700 km) and apogee (20 000 km). Distribution functions in the main analyzer half-plane are obtained after a complete scan of windows and energies with temporal resolution between one and a few seconds. Three-dimensional (3D) distributions are measured in one spacecraft spin period (120 s). The secondary analyzer has a much smaller geometrical factor, but offers partial access to the 3D dependence of the distributions with a few seconds temporal resolution. Preliminary results are presented. Simultaneous, local heating of both H+ and O+ ions resulting in conical distributions below 80 eV is observed up to 3 Earth's radii altitudes. The thermal ion signatures associated with large-scale nightside magnetospheric boundaries are investigated and a new ion outflow feature is identified associated to the polar edge of the auroral oval. Detailed distribution functions of injected magnetosheath ions and ouflowing cleft fountain ions are measured down to a few eVs in the dayside