Influence of morning maternal care on the behavioural responses of 8-week-old Beagle puppies to new environmental and social stimuli

In mammals, maternal care represents a major constituent of the early-life environment and its influence on individual development has been documented in rodents, non-human primates, humans and recently in adult dogs. The quality and quantity of mother-offspring interactions exerts a multilevel regulation upon the physiological, cognitive, and behavioural development of the offspring. For example, in rats variations in maternal behaviour, such as mother-pup body contact and the amount of licking towards pups in the nest during the early days after parturition, influences the endocrine, emotional, and behavioural responses to stress in the offspring. This produces long-term consequences, which may remain into adulthood and can be transmitted to subsequent generations. Literature about maternal care in dogs and its effect on puppy behaviour is still scarce, although the topic is receiving a growing interest. The aim of the present study was to determine the effects of morning maternal care on behavioural responses of puppies to new environmental and social stimuli. In order to achieve this, maternal care (licking, ano-genital licking, nursing and mother-puppy contact) was assessed in eight litters of domestic dogs living in standard rearing conditions during the first three weeks post-partum. Puppies were subjected to two behavioural tests (arena and isolation tests) at 58-60 days of age, and their behavioural responses were video recorded and analysed. Data was analysed using multivariate analyses (PCA, PLS).During the isolation test, a higher level of maternal care was associated with more exploration and a higher latency to emit the first yelp; on the contrary, a lower level of maternal care was associated with increased locomotion, distress vocalisations and destructive behaviours directed at the enclosure.These results, comparable to those reported in laboratory rat models and to some extent to those recently reported in dog literature, highlight the importance of maternal care on the behavioural development of domestic dog puppies

Development of the dog attachment insecurity screening inventory (D-aisi):A pilot study on a sample of female owners

SIMPLE SUMMARY: The Strange Situation Procedure is a laboratory test originally designed to assess the quality of a child’s attachment bond to their mother and is widely used in dogs to assess their attachment bond towards the owner. However, the SSP is time consuming and limits the amount and variety of obtainable data. In order to overcome these limitations, we adapted a three-dimensional parent-report scale, named the Attachment Insecurity Screening Inventory (AISI) 6–12, originally developed to assess 6- to 12-year-old children’s attachment insecurity, to dog–owner dyads, and we assessed scale consistency and validity. A first statistical analysis performed on the responses provided by 524 female owners to the online questionnaire revealed five scale dimensions named “physical contact”, “control”, separation anxiety”, “owner as emotional support”, and “owner as a source of positive emotion”. However, a further forced extraction of three components resulted in subscales that mirrored the ones reported for the original AISI in terms of item composition (i.e., ambivalent, avoidant, and disorganized). The three subscales also had satisfactory to good measures of internal reliability. The final scale was named the Dog Attachment Insecurity Screening Inventory (D-AISI). Although promising, it needs to be refined and tested for more validity measures. ABSTRACT: To date, the Strange Situation Procedure is the only tool available to investigate the quality of the dog’s attachment bond towards the owner. This study aimed to adapt a parent-report scale, named the Attachment Insecurity Screening Inventory (AISI) 6–12, originally designed to assess 6- to 12-year-old children’s attachment insecurity, to dog–owner dyads and assess measures of consistency and validity. The online questionnaire was completed by 524 female dog owners. Principal component analysis (PCA) revealed five components named, respectively, “physical contact”, “control”, “separation anxiety”, “owner as emotional support”, and “owner as a source of positive emotion”. Because of the three-factor structure of the original AISI, a PCA with a pre-fixed set of three factors was also performed. The resulting subscales mirrored the ones found for the original scale (i.e., ambivalent, avoidant, and disorganized), although four items did not fit the model. Internal reliability appeared to be satisfying for the ambivalent and the disorganized subscales, and good for the avoidant subscale. The theoretical background and the results of this study suggest that the three-dimensional model represents a better solution for the interpretation of the Dog Attachment Insecurity Screening Inventory (D-AISI). Although promising, this scale requires refinement and assessment of additional validity measures

Poly(Alkylene 2,5-thiophenedicarboxylate) polyesters: A new class of bio-based high-performance polymers for sustainable packaging

In the present study, 100% bio-based polyesters of 2,5-thiophenedicarboxylic acid were synthesized via two-stage melt polycondensation using glycols containing 3 to 6 methylene groups. The so-prepared samples were characterised from the molecular point of view and processed into free-standing thin films. Afterward, both the purified powders and the films were subjected to structural and thermal characterisation. In the case of thin films, mechanical response and barrier properties to O2 and CO2 were also evaluated. From the results obtained, it emerged that the length of glycolic sub-units is an effective tool to modulate the chain mobility and, in turn, the kind and amount of ordered phases developed in the samples. In addition to the usual amorphous and 3D crystalline phases, in all the samples investigated it was possible to evidence a further phase characterised by a lower degree of order (mesophase) than the crystalline one, whose amount is strictly related to the glycol sub-unit length. The relative fraction of all these phases is responsible for the different mechanical and barrier performances. Last, but not least, a comparison between thiophene-based homopolymers and their furan-based homologues was carried out

Heterogeneity of neuroinflammatory responses in amyotrophic lateral sclerosis: A challenge or an opportunity?

Amyotrophic Lateral Sclerosis (ALS) is a complex pathology: (i) the neurodegeneration is chronic and progressive; it starts focally in specific central nervous system (CNS) areas and spreads to different districts; (ii) multiple cell types further than motor neurons (i.e., glial/immune system cells) are actively involved in the disease; (iii) both neurosupportive and neurotoxic neuroinflammatory responses were identified. Microglia cells (a key player of neuroinflammation in the CNS) attracted great interest as potential target cell population that could be modulated to counteract disease progression, at least in preclinical ALS models. However, the heterogeneous/multifaceted microglia cell responses occurring in different CNS districts during the disease represent a hurdle for clinical translation of single-drug therapies. To address this issue, over the past ten years, several studies attempted to dissect the complexity of microglia responses in ALS. In this review, we shall summarize these results highlighting how the heterogeneous signature displayed by ALS microglia reflects not only the extent of neuronal demise in different regions of the CNS, but also variable engagement in the attempts to cope with the neuronal damage. We shall discuss novel avenues opened by the advent of single-cell and spatial transcriptomics technologies, underlining the potential for discovery of novel therapeutic targets, as well as more specific diagnostic/prognostic not-invasive markers of neuroinflammation

Bicolor Pixels from a Single Active Molecular Material by Surface-Tension-Driven Deposition†

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Characterisation of the Galactic thin disc with Corot targets

We use kinematical and chemical properties of 754 Corot stars to characterise the stellar populations of the Milky Way disc in three beams close the Galactic plane. From the atmospheric parameters derived in Gazzano et al. (2010) with the Matisse algorithm, we derived stellar distances using isochrones. Combining these data with proper motions, we provide the complete kinematical description of stars in three Corot fields. Finally, we used kinematical criteria to identify the Galactic populations in our sample and study their characteristics, particularly their chemistry. Comparing our kinematics with the Besancon Galactic model, we show that, within 3-sigma, simulated and observed kinematical distributions are in good agreement. We study the characteristics of the thin disc, finding a correlation that is significant at a value of 2-sigma between the V-velocity component and the metallicity for two different radial distance bins (8-9kpc and 9-10kpc; but not for the most inner bin 7-8kpc, probably because of the uncertainties in the abundances) which could be interpreted as radial migration evidence. We also measured a radial metallicity gradient value of -0.097+/-0.015dex/kpc with giant stars, and -0.053+/-0.015dex/kpc with dwarfs. Finally, we identified metal-rich stars with peculiar high [alpha/Fe] values in the directions pointing to the inner part of the Galaxy. Applying the same methodology to the planet-hosting stars detected by Corot shows that they mainly belong to the thin disc population with normal chemical and kinematical properties.Comment: Astronomy & Astrophysics, in pres

Entangling quantum-logic gate operated with an ultrabright semiconductor single-photon source

We demonstrate the unambiguous entangling operation of a photonic quantum-logic gate driven by an ultrabright solid-state single-photon source. Indistinguishable single photons emitted by a single semiconductor quantum dot in a micropillar optical cavity are used as target and control qubits. For a source brightness of 0.56 photons per pulse, the measured truth table has an overlap with the ideal case of 68.4 +/- 0.5%, increasing to 73.0 +/- 1.6% for a source brightness of 0.17 photons per pulse. The gate is entangling: At a source brightness of 0.48, the Bell-state fidelity is above the entangling threshold of 50% and reaches 71.0 +/- 3.6% for a source brightness of 0.15