Gender in agricultural mechanization: Key guiding questions

This tool describes case studies of gender implications in mechanization in RTB crops, illustrating both positive and negative outcomes. GENNOVATE research has shown that the beneficiaries of mechanization tend to be the wealthier rather than the poorer farmers and more often men than women. However, results also reveal that women have strong interest in mechanization as a way to improve their own circumstances

Innovación agrícola pro-pobre para la seguridad y soberanía alimentaria en la región andina: el caso IssAndes en Ecuador.

El proyecto "Innovación para la seguridad y la soberanía alimentaria en la región andina"- IssAndes, se inició en marzo del 2011 y culminó en marzo del 2015. El proyecto fue ejecutado en Bolivia, Ecuador, Perú y Colombia, con socios de agricultura, salud y educación en cada país. Fue coordinado por el Centro Internacional de la Papa (CIP) y financiado por la Unión Europea a través del Fondo Internacional de Desarrollo Agrícola (FIDA). El objetivo de IssAndes fue fortalecer la innovación para la seguridad alimentaria a diferentes niveles territoriales (local, nacional y regional) en la región andina, en respuesta a las necesidades de los grupos rurales vulnerables y así contribuir a la reducción de la desnutrición de niño/as menores de tres años mediante mejoras en la alimentación y nutrición de las familias. En el Ecuador, el proyecto se implementó en las provincias de Chimborazo, Tungurahua y Cotopaxi, en un total de 10 cantones y 17 parroquias, llegando a 44 comunidades con 1135 familias vinculadas al rubro papa. El proyecto tuvo un total de siete socios a nivel nacional (Instituto Nacional Autónomo de Investigaciones Agropecuarias - INIAP, Escuela Superior Politécnica de Chimborazo - ESPOCH, Visión Mundial, Estrategia Acción Nutrición - EAN, Oficina para Estudios del Agro - OfiAgro, Centro Latinoamericano para el Desarrollo Rural-RIMISP y la Fundación Minga para la Acción Rural y la Cooperación - MARCO) y dos socios a nivel regional (CIP y el Instituto de Investigación Nutricional - IIN). IssAndes estructuró sus acciones de investigación y desarrollo en cuatro componentes: i) Contribución de la papa a la nutrición y salud; ii) Mejoras en los sistemas de producción basados en papa; iii) Educación nutricional para el cambio de comportamiento; e iv) Incidencia pública y política

Replant problems in South Tyrol: role of fungal pathogens and microbial populations in conventional and organic apple orchards

South Tyrol, the main Italian apple growing area, is characterised by an highly intensive soil cultivation. Previous investigations shows the existence of replant disorders although it has not been evaluated which are the main causes. A survey has been carried out in this area with two main aims I) to evaluate the role of soil borne pathogens in apple replant disease and II) to evaluate the effect of soil management toward soil borne pathogens causing replant diseases. The experimental sites were chosen in order to obtain three couples of contiguous conventional and organic apple orchards. Soil sickness test with young apple plants gave a significant growth reduction in all soil samples if compared to a peat control. Among all root colonising fungi (Fusarium oxysporum, F. solani, Aphanomyces sp., Cy/incrocarpon sp., Rhizoctonia sp. and Pythium sp.) some Rhizoctonia solani strains and all Pythium spp. were the most pathogenic. In all cases organic management seems to reduce the soil sickness severity caused by root rot fungal pathogens

Pasta consumption and connected dietary habits: Associations with glucose control, adiposity measures, and cardiovascular risk factors in people with type 2 diabetes—TOSCA.IT study

Background: Pasta is a refined carbohydrate with a low glycemic index. Whether pasta shares the metabolic advantages of other low glycemic index foods has not really been investigated. The aim of this study is to document, in people with type-2 diabetes, the consumption of pasta, the connected dietary habits, and the association with glucose control, measures of adiposity, and major cardiovascular risk factors. Methods: We studied 2562 participants. The dietary habits were assessed with the European Prospective Investigation into Cancer and Nutrition (EPIC) questionnaire. Sex-specific quartiles of pasta consumption were created in order to explore the study aims. Results: A higher pasta consumption was associated with a lower intake of proteins, total and saturated fat, cholesterol, added sugar, and fiber. Glucose control, body mass index, prevalence of obesity, and visceral obesity were not significantly different across the quartiles of pasta intake. No relation was found with LDL cholesterol and triglycerides, but there was an inverse relation with HDL-cholesterol. Systolic blood pressure increased with pasta consumption; but this relation was not confirmed after correction for confounders. Conclusions: In people with type-2 diabetes, the consumption of pasta, within the limits recommended for total carbohydrates intake, is not associated with worsening of glucose control, measures of adiposity, and major cardiovascular risk factors

Immunoinformatic analysis of the SARS-CoV-2 envelope protein as a strategy to assess cross-protection against COVID-19

Envelope protein of coronaviruses is a structural protein existing in both monomeric and homo-pentameric form. It has been related to a multitude of roles including virus infection, replication, dissemination and immune response stimulation. In the present study, we employed an immunoinformatic approach to investigate the major immunogenic domains of the SARS-CoV-2 envelope protein and map them among the homologue proteins of coronaviruses with tropism for animal species that are closely inter-related with the human beings population all over the world. Also, when not available, we predicted the envelope protein structural folding and mapped SARS-CoV-2 epitopes. Envelope sequences alignment provides evidence of high sequence homology for some of the investigated virus specimens; while the structural mapping of epitopes resulted in the interesting maintenance of the structural folding and epitope sequence localization also in the envelope proteins scoring a lower alignment score. In line with the One-Health approach, our evidences provide a molecular structural rationale for a potential role of taxonomically related coronaviruses in conferring protection from SARS-CoV-2 infection and identifying potential candidates for the development of diagnostic tools and prophylactic-oriented strategies

Hormone replacement therapy enhances IGF-1 signaling in skeletal muscle by diminishing miR-182 and miR-223 expressions: a study on postmenopausal monozygotic twin pairs

MiRNAs are fine-tuning modifiers of skeletal muscle regulation, but knowledge of their hormonal control is lacking. We used a co-twin case-control study design, that is, monozygotic postmenopausal twin pairs discordant for estrogen-based hormone replacement therapy (HRT) to explore estrogen-dependent skeletal muscle regulation via miRNAs. MiRNA profiles were determined from vastus lateralis muscle of nine healthy 54-62-years-old monozygotic female twin pairs discordant for HRT (median 7 years). MCF-7 cells, human myoblast cultures and mouse muscle experiments were used to confirm estrogen's causal role on the expression of specific miRNAs, their target mRNAs and proteins and finally the activation of related signaling pathway. Of the 230 miRNAs expressed at detectable levels in muscle samples, qPCR confirmed significantly lower miR-182, miR-223 and miR-142-3p expressions in HRT using than in their nonusing co-twins. Insulin/IGF-1 signaling emerged one common pathway targeted by these miRNAs. IGF-1R and FOXO3A mRNA and protein were more abundantly expressed in muscle samples of HRT users than nonusers. In vitro assays confirmed effective targeting of miR-182 and miR-223 on IGF-1R and FOXO3A mRNA as well as a dose-dependent miR-182 and miR-223 down-regulations concomitantly with up-regulation of FOXO3A and IGF-1R expression. Novel finding is the postmenopausal HRT-reduced miRs-182, miR-223 and miR-142-3p expression in female skeletal muscle. The observed miRNA-mediated enhancement of the target genes' IGF-1R and FOXO3A expression as well as the activation of insulin/IGF-1 pathway signaling via phosphorylation of AKT and mTOR is an important mechanism for positive estrogen impact on skeletal muscle of postmenopausal women