Bioethanol Production From Tropical Marine Microalgae Ambon Bay Navicula sp. of The Inner Ambon Bay Strain

Microalgae are photosynthetic microorganisms that contain carbohydrates can be converted into glucose through a hydrolysis process so that they can be used as raw materials for bioethanol production. Navicula sp. strain TAD is a type of marine microalgae that is spread in the inner Ambon Bay (TAD) sea waters and is used to manufacture bioethanol. This study aims to determine the content of carbohydrates and bioethanol produced from tropical marine microalgae Ambon bay Navicula sp. strain TAD. The research was conducted through several stages, namely the cultivation of Navicula sp. strain TAD to obtain biomass, determine carbohydrate content in biomass, hydrolyze biomass and manufacture bioethanol through a fermentation process using Saccharomyces cerevisiae. The results showed that Navicula sp. strain TAD cultivation for seven days had the highest cell density of 72.167 × 105 ± 0.946 cells  mL-1 with dry biomass of 0.933 ± 0.062 g and productivity of 0.166 ± 0.011 gL-1 hour-1. The sulfuric acid hydrolysis process was analyzed for a sugar content of 146.5695 ± 0.758 mg. A fermentation process follows the results of the hydrolysis to produce bioethanol. The bioethanol content obtained was 6.357%. These indicated that Navicula sp. strain TAD has the potential to produce bioethano

Bioethanol Production From Tropical Marine Microalgae Ambon Bay Navicula sp. of The Inner Ambon Bay Strain

Microalgae are photosynthetic microorganisms that contain carbohydrates can be converted into glucose through a hydrolysis process so that they can be used as raw materials for bioethanol production. Navicula sp. strain TAD is a type of marine microalgae that is spread in the inner Ambon Bay (TAD) sea waters and is used to manufacture bioethanol. This study aims to determine the content of carbohydrates and bioethanol produced from tropical marine microalgae Ambon bay Navicula sp. strain TAD. The research was conducted through several stages, namely the cultivation of Navicula sp. strain TAD to obtain biomass, determine carbohydrate content in biomass, hydrolyze biomass and manufacture bioethanol through a fermentation process using Saccharomyces cerevisiae. The results showed that Navicula sp. strain TAD cultivation for seven days had the highest cell density of 72.167 × 105 ± 0.946 cells  mL-1 with dry biomass of 0.933 ± 0.062 g and productivity of 0.166 ± 0.011 gL-1 hour-1. The sulfuric acid hydrolysis process was analyzed for a sugar content of 146.5695 ± 0.758 mg. A fermentation process follows the results of the hydrolysis to produce bioethanol. The bioethanol content obtained was 6.357%. These indicated that Navicula sp. strain TAD has the potential to produce bioethano

Faktor Risiko dengan Angka Kejadian Post Operative Nausea and Vomiting pada Pasien yang Dilakukan Anestesi Spinal di RSUD Dr. M. Haulussy Ambon dan RS Bhayangkara Ambon Tahun 2022

Abstrak Pendahuluan: Postoperative Nausea and Vomiting (PONV) atau mual dan muntah pascaoperasi merupakan efek samping yang sering terjadi setelah dilakukan tindakan anestesi. PONV dihubungkan dengan berbagai faktor, dan faktor tersebut dikategorikan ke dalam faktor risiko yang berhubungan dengan pasien, pembedahan, dan anestesi. PONV dapat terjadi pada laki-laki dan perempuan di semua kalangan usia. Meski belum diketahui secara pasti, kebiasaan merokok dipercaya dapat mencegah terjadinya PONV. Selain itu, pemberian profilaksis ondansetron juga dapat mencegah terjadinya PONV. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan faktor risiko dengan angka kejadian PONV pada pasien yang dilakukan anestesi spinal di RSUD Dr. M. Haulussy Ambon dan RS Bhayangkara Ambon tahun 2022. Metode: Penelitian ini merupakan penelitian analitik observasional dengan pendekatan cross sectional yang dilakukan pada Februari-Maret 2022 dengan menggunakan teknik pengambilan sampel total sampling sebanyak 43 pasien yang menjalani pembedahan lower abdomen dan dilakukan anestesi spinal di RSUD Dr. M. Haulussy Ambon dan RS Bhayangkara Ambon. Instrumen yang digunakan adalah kuesioner yang diambil dari artikel “Simplified Postoperative Nausea and Vomiting Impact Scale for Audit and Post-Discharge Review dalam British Journal of Anaesthesia” yang telah dimodifikasi. Data dianalisis dengan menggunakan uji Chi-Square untuk mengetahui hubungan faktor risiko dengan angka kejadian PONV pada pasien yang dilakukan anestesi spinal. Hasil: Hasil penelitian menunjukan bahwa ada hubungan yang signifikan antara umur (P=0,027), jenis kelamin (P=0,008), riwayat merokok (P=0,011), dan riwayat profilaksis ondansetron (P=0,005) dengan kejadian PONV pada pasien pascaoperasi lower abdomen dengan anestesi spinal. Kesimpulan: Penelitian ini menunjukkan pentingnya mengetahui faktor risiko untuk mewaspadai terjadinya PONV pascaoperasi lower abdomen dengan anestesi spinal di RSUD Dr. M. Haulussy Ambon dan RS Bhayangkara Ambon. Kata Kunci: PONV, Umur, Jenis Kelamin, Riwayat Merokok, Profilaksis Ondansetron       Abstract Introduction: Postoperative Nausea and Vomiting (PONV) is a side effect that often occurs after anesthesia. PONV is associated with various factors, these factors are categorized into risk factors related to patient, surgery, and anesthesia. PONV can occur in men and women of all ages. Although it is not known for certain, smoking is believed to prevent the occurrence of PONV. In addition, prophylactic administration of ondansetron can also prevent the occurrence of PONV. Objective: This study aims to determine the relationship between risk factors and the incidence of PONV in patients undergoing spinal anesthesia at Dr. M. Haulussy Ambon and Bhayangkara Hospital Ambon in 2022. Methods: This study is an observational analytic study with a cross sectional approach which was conducted in February-March 2022 by using a total sampling technique of 43 patients who underwent lower abdominal surgery and underwent spinal anesthesia at Dr. Hospital. M. Haulussy Ambon and Bhayangkara Hospital Ambon. The instrument used is a questionnaire taken from the article “Simplified Postoperative Nausea and Vomiting Impact Scale for Audit and Post-Discharge Review in the British Journal of Anaesthesia” that has been modified. Data were analyzed using the Chi-Square test to determine the relationship between risk factors and the incidence of PONV in patients undergoing spinal anesthesia. Results: The results showed that there was a significant relationship between age (P=0.027), gender (P=0.008), smoking history (P=0.011), and history of ondansetron prophylaxis (P=0.005) with the incidence of PONV in patients after lower abdominal surgery. with spinal anesthesia. Conclusions: This study shows the importance of knowing the risk factors to be aware of the occurrence of PONV after lower abdominal surgery with spinal anesthesia at Dr. M. Haulussy Ambon and Bhayangkara Hospital Ambon. Key words: PONV, Age, Gender, Smoking, Ondansetron Prophylaxi

Bioethanol Production From Tropical Marine Microalgae Ambon Bay Navicula sp. of The Inner Ambon Bay Strain

Microalgae are photosynthetic microorganisms that contain carbohydrates can be converted into glucose through a hydrolysis process so that they can be used as raw materials for bioethanol production. Navicula sp. strain TAD is a type of marine microalgae that is spread in the inner Ambon Bay (TAD) sea waters and is used to manufacture bioethanol. This study aims to determine the content of carbohydrates and bioethanol produced from tropical marine microalgae Ambon bay Navicula sp. strain TAD. The research was conducted through several stages, namely the cultivation of Navicula sp. strain TAD to obtain biomass, determine carbohydrate content in biomass, hydrolyze biomass and manufacture bioethanol through a fermentation process using Saccharomyces cerevisiae. The results showed that Navicula sp. strain TAD cultivation for seven days had the highest cell density of 72.167 × 105 ± 0.946 cells  mL-1 with dry biomass of 0.933 ± 0.062 g and productivity of 0.166 ± 0.011 gL-1 hour-1. The sulfuric acid hydrolysis process was analyzed for a sugar content of 146.5695 ± 0.758 mg. A fermentation process follows the results of the hydrolysis to produce bioethanol. The bioethanol content obtained was 6.357%. These indicated that Navicula sp. strain TAD has the potential to produce bioethano

Analysis of dark matter profiles in the halos of spiral galaxies

The distribution of dark matter in the low surface brightness spiral galaxies U5750, U11454, U11819, and U11648 is analyzed by utilizing well-established density profiles from existing literature. These profiles include the Beta, Brownstein, Burkert, exponential sphere, pseudo-isothermal, and Persic profiles. For simplicity, we assume spherically symmetric distribution of dark matter in the considered galaxies, without accounting for their intricate structural complexities. For each density profile, we fit the rotation curve data to the aforementioned galaxies. Employing Markov Chain Monte Carlo statistical analyses, we infer model-free parameters from the rotation curves, namely the characteristic (central) density and scale radius. This enables us to estimate the total dark matter mass within the galactic halos. To identify the best-fit profile, we employ the Bayesian Information Criterion. Additionally, we adopt the Levenberg-Marquardt nonlinear least squares approach for the purpose of comparison and completeness. Through our statistical analysis, we offer a physical interpretation behind the selection of specific profiles. This interpretation is based on the insights obtained from the analyses

The investigation of chlorpyrifos (Cpy) detection of PEDOT:PSS-MXene(Ti2CTX)-BSA-GO composite using P-ISFET reduction method

MXenes are two-dimensional materials that are attractive for applications as sensors because they possess high conductivity, super hydrophilicity and high surface area. There already exist substantial researches on the use of Ti3C2Tx based MXenes as electrochemical biosensors, but in contrast Ti2CTX based MXenes are rarely discussed due to their inherent resistance instability. However, the use of Ti2CTX based MXenes is still worth exploring as theoretical studies have shown that Ti2CTX possesses a significantly lower bandgap compared to many other MXenes structures. Herein, this study examines the use of Ti2CTX MXene structures in a P-channel ion-sensitive field-effect transistor (P-ISFET) for the detection of Chlorpyrifos (Cpy). Compositing the PEDOT:PSS thin film with delaminated Ti2CTX MXenes flakes with graphene oxide (GO) and bovine serum albumin (BSA) allows it to maintain its sheet resistance at around 100 kOhm for 3 days. Interestingly when using the composite thin film, the minimum threshold voltage required to observe Cpy electroreduction is −0.1 V. This is much lower than that when using titanium dioxide (TiO2), which is −1.5 V. Composite thin films containing Ti2CTX MXene are found to detect Cpy with higher sensitivity compared to thin films without MXene. This is because the presence of Mxene in the PEDOT:PSS composite thin films improves the surface area available for Cpy detection. This study highlights the potential of Ti2CTx MXene-BSA composite as a promising 2D material for enzyme-free CPY detection

Knowledge network modelling to support decision-making for strategic intervention in IT project-oriented change management

This is the Accepted Manuscript version of an article published by Taylor & Francis in Journal of Decision Systems on 20 March 2014, available online: http://www.tandfonline.com/doi/abs/10.1080/12460125.2014.886499.This paper focuses on knowledge management to enhance decision support systems for strategic intervention in information technology (IT) project-oriented change management. It proposes a model of change management knowledge networks (CMKNM) to support decision by tackling three existing issues: insufficient knowledge traceability based on the relationships between knowledge elements and key factors, lack of procedural knowledge to provide adequate policies to guide changes, and lack of ‘lessons learned’ documentation in knowledge bases. A qualitative method was used to investigate issues surrounding knowledge mobilisation and knowledge networks. Empirical study was undertaken with industries to test the CMKNM. Results are presented from the empirical study on the key factors influencing knowledge mobilisation in IT project-oriented change management, knowledge networks and connections. The CMKNM model allows key knowledge mobilisation factors to be aligned with each other; it also defines the connections between knowledge networks allowing knowledge to be mobilised by tracing knowledge channels to support decision.Peer reviewe

A spill over effect of entrepreneurial orientation on technological innovativeness:an outlook of universities and research based spin offs

partially_open5siBy shifting towards Romer’s (Am Econ Rev 94:1002–1037, 1986) economy and so the spread of knowledge economy, universities started to adopt a collaborative approach with their entrepreneurial ecosystem. They turn out to be risk taker, autonomous, proactive, competitive, and innovative. In a nutshell, they are entrepreneurial oriented with the aim to generate new innovative ventures, known as research-based spin offs. Doubly, this has induced an improvement of technology transfer and the degree of entrepreneurship in the current knowledge economy. However there still is a paucity of studies on the spill over effect of entrepreneurial orientated universities and research-based spin off on technology transfer need to be more explored. Therefore, the article investigates the link between entrepreneurial orientation and such spill overs by offering an outlook of two universities and two research-based spin offs in the United Kingdom. The scope is to provide a deep view of technological innovativeness in a research context, entrepreneurial oriented. Our research suggests that entrepreneurial attitude has become an imperative to succeed in the context where British institutions currently operate. Entrepreneurship brings the necessary technological innovation to the university and its students, which results in better positioning of the university at national and international levels, with the subsequent impact on their ability to attract not only new students and academics but also funding to conduct their research.openScuotto, Veronica; Del Giudice, Manlio; Garcia-Perez, Alexeis; Orlando, Beatrice; Ciampi, FrancescoScuotto, Veronica; Del Giudice, Manlio; Garcia-Perez, Alexeis; Orlando, Beatrice; Ciampi, Francesc

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Evidence on the magnitude of the economic, health and population effects of palm cooking oil consumption: an integrated modelling approach with Thailand as a case study

BACKGROUND: Palm oil’s high yields, consequent low cost and highly versatile properties as a cooking oil and food ingredient have resulted in its thorough infiltration of the food sector in some countries. Longitudinal studies have associated palm oil’s high saturated fatty acid content with non-communicable disease, but neither the economic or disease burdens have been assessed previously. // METHODS: This novel palm oil-focussed disease burden assessment employs a fully integrated health, macroeconomic and demographic Computable General Equilibrium Model for Thailand with nine regional (urban/rural) households. Nutritional changes from food consumption are endogenously translated into health (myocardial infarction (MI) and stroke) and population outcomes and are fed back into the macroeconomic model as health and caregiver-related productive labour supply effects and healthcare costs to generate holistic 2016–2035 burden estimates. Model scenarios mirror the replacement of palm cooking oil with other dietary oils and are compared with simulated total Thai health and macroeconomic burdens for MI and stroke. // RESULTS: Replacing consumption of palm cooking oil with other dietary oils could reduce MI/stroke incident cases by 8280/2639 and cumulative deaths by 4683/894 over 20 years, removing approximately 0.5% of the total Thai burden of MI/stroke. This palm cooking oil replacement would reduce consumption shares of saturated/monounsaturated fatty acids in Thai household consumption by 6.5%/3% and increase polyunsaturated fatty acid consumption shares by 14%, yielding a 1.74% decrease in the population-wide total-to-HDL cholesterol ratio after 20 years. The macroeconomic burden that would be removed is US$308mn, approximately 0.44% of the total burden of MI/stroke on Thailand’s economy or 0.003% of cumulative 20-year GDP. Bangkok and Central region households benefit most from removal of disease burdens. // CONCLUSIONS: Simulations indicate that consumption of palm cooking oil, rather than other dietary oils, imposes a negative health burden (MI and stroke) and associated economic burden on a high consuming country, such as Thailand. Integrated sectoral model frameworks to assess these burdens are possible, and burden estimates from our simulated direct replacement of palm cooking oil indicate that using these frameworks both for broader analyses of dietary palm oil use and total burden analyses of other diseases may also be beneficial