4,340 research outputs found
Location and Conformation of the LK alpha 14 Peptide in Water/Ethanol Mixtures
It is widely recognized that solvation is one of the major factors determining structure and functionality of proteins and long peptides, however it is a formidable challenge to address it both experimentally and computationally. For this reason, simple peptides are used to study fundamental aspects of solvation. It is well established that alcohols can change the peptide conformation and tuning of the alcohol content in solution can dramatically affect folding and, as a consequence, the function of the peptide. In this work, we focus on the leucine and lysine based LK alpha 14 peptide designed to adopt an alpha-helical conformation at an apolar-polar interface. We investigate LK alpha 14 peptide's bulk and interfacial behavior in water/ethanol mixtures combining a suite of experimental techniques (namely, circular dichroism and nuclear magnetic resonance spectroscopy for the bulk solution, surface pressure measurements and vibrational sum frequency generation spectroscopy for the air-solution interface) with molecular dynamics simulations. We observe that ethanol highly affects both the peptide location and conformation. At low ethanol content LK alpha 14 lacks a clear secondary structure in bulk and shows a clear preference to reside at the air-solution interface. When the ethanol content in solution increases, the peptide's interfacial affinity is markedly reduced and the peptide approaches a stable alpha-helical conformation in bulk facilitated by the amphiphilic nature of the ethanol molecules
Assessing the vulnerability of buildings to tsunami in Sydney
Australia is vulnerable to the impacts of tsunamis and exposure along the SE coast of New South Wales is especially high. Significantly, this is the same area reported to have been affected by repeated large magnitude tsunamis during the Holocene. Efforts are under way to complete probabilistic risk assessments for the region but local government planners and emergency risk managers need information now about building vulnerability in order to develop appropriate risk management strategies. We use the newly revised PTVA-3 Model (Dall'Osso et al., 2009) to assess the relative vulnerability of buildings to damage from a "worst case tsunami" defined by our latest understanding of regional risk – something never before undertaken in Australia. We present selected results from an investigation of building vulnerability within the local government area of Manly – an iconic coastal area of Sydney. We show that a significant proportion of buildings (in particular, residential structures) are classified as having "High" and "Very High" Relative Vulnerability Index scores. Furthermore, other important buildings (e.g., schools, nursing homes and transport structures) are also vulnerable to damage. Our results have serious implications for immediate emergency risk management, longer-term land-use zoning and development, and building design and construction standards. Based on the work undertaken here, we recommend further detailed assessment of the vulnerability of coastal buildings in at risk areas, development of appropriate risk management strategies and a detailed program of community engagement to increase overall resilience
Corrigendum to "A revised (PTVA) model for assessing the vulnerability of buildings to tsunami damage" published in Nat. Hazards Earth Syst. Sci., 9, 1557–1565, 2009"
No abstract available
Noise reduction in muon tomography for detecting high density objects
The muon tomography technique, based on multiple Coulomb scattering of cosmic
ray muons, has been proposed as a tool to detect the presence of high density
objects inside closed volumes. In this paper a new and innovative method is
presented to handle the density fluctuations (noise) of reconstructed images, a
well known problem of this technique. The effectiveness of our method is
evaluated using experimental data obtained with a muon tomography prototype
located at the Legnaro National Laboratories (LNL) of the Istituto Nazionale di
Fisica Nucleare (INFN). The results reported in this paper, obtained with real
cosmic ray data, show that with appropriate image filtering and muon momentum
classification, the muon tomography technique can detect high density
materials, such as lead, albeit surrounded by light or medium density material,
in short times. A comparison with algorithms published in literature is also
presented
In Situ Label-Free Study of Protein Adsorption on Nanoparticles
[Image: see text] Improving the design of nanoparticles for use as drug carriers or biosensors requires a better understanding of the protein–nanoparticle interaction. Here, we present a new tool to investigate this interaction in situ and without additional labeling of the proteins and/or nanoparticles. By combining nonresonant second-harmonic light scattering with a modified Langmuir model, we show that it is possible to gain insight into the adsorption behavior of blood proteins, namely fibrinogen, human serum albumin, and transferrin, onto negatively charged polystyrene nanoparticles. The modified Langmuir model gives us access to the maximum amount of adsorbed protein, the apparent binding constant, and Gibbs free energy. Furthermore, we employ the method to investigate the influence of the nanoparticle size on the adsorption of human serum albumin and find that the amount of adsorbed protein increases more than the surface area per nanoparticle for larger diameters
String tension in gonihedric 3D Ising models
For the 3D gonihedric Ising models defined by Savvidy and Wegner the bare
string tension is zero and the energy of a spin interface depends only on the
number of bends and self-intersections, in antithesis to the standard
nearest-neighbour 3D Ising action. When the parameter kappa weighting the
self-intersections is small the model has a first order transition and when it
is larger the transition is continuous.
In this paper we investigate the scaling of the renormalized string tension,
which is entirely generated by fluctuations, using Monte Carlo simulations This
allows us to obtain an estimate for the critical exponents alpha and nu using
both finite-size-scaling and data collapse for the scaling function.Comment: Latex + postscript figures. 8 pages text plus 7 figures, spurious
extra figure now removed
Optical absorption and photoluminescence spectroscopy of the growth of silver nanoparticles
Results obtained from the optical absorption and photoluminescence (PL)
spectroscopy experiments have shown the formation of excitons in the
silver-exchanged glass samples. These findings are reported here for the first
time. Further, we investigate the dramatic changes in the photoemission
properties of the silver-exchanged glass samples as a function of postannealing
temperature. Observed changes are thought to be due to the structural
rearrangements of silver and oxygen bonding during the heat treatments of the
glass matrix. In fact, photoelectron spectroscopy does reveal these chemical
transformations of silver-exchanged soda glass samples caused by the thermal
effects of annealing in a high vacuum atmosphere. An important correlation
between temperature-induced changes of the PL intensity and thermal growth of
the silver nanoparticles has been established in this Letter through precise
spectroscopic studies.Comment: 15 pages,4 figures,PDF fil
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