Geographic Variation in Wood Specific Gravity: Effects of Latitude, Temperature, and Precipitation

Wood basic specific gravity (SG) was compared at sites located along a gradient from 52°N latitude to the equator. Mean SG increased by 0.0049 per °C mean annual temperature (MAT), and decreased by 0.00017 per cm of mean annual precipitation (MAP). Considered alone, MAT was a better predictor of mean SG across the temperate zone (3-22°C MAT, latitude north of 29°N; r2 = 0.80) than it was across the entire MAT range (r2 = 0.62) or across warm tropical sites alone (MAT > 23°C; r2 = 0.33, p = 0.67). In contrast, MAP considered alone was a better predictor of mean SG in the warm tropical sites (r2 = 0.62) than across all sites (r2 = 0.04, p = 0.39).Variability in SG among the sites was compared using two measures of dispersion: range and standard deviation. As MAT increased across the temperate zone, maximum SG increased and minimum SG remained constant, resulting in an increase in SG range; SG standard deviation, however, remained constant. Both SG range and SG standard deviation increased dramatically in the warm tropical zone relative to the temperate zone, demonstrating that variability in SG in the warm tropics is much greater than would be predicted from greater species richness alone

Extreme Radial Changes in Wood Specific Gravity in Some Tropical Pioneers

Twelve Hampea appendiculata, six Heliocarpus appendiculatus, and twelve Ochroma pyramidale trees from tropical wet forest in Costa Rica were sampled across their radii. Wood from all three species increased linearly in specific gravity from pith to bark. The magnitude of the increase was about 0.1 units of specific gravity per 10 cm of radius, although there were differences between the species and between trees within each species. All three species colonize clearings and disturbed sites, and these extreme changes in specific gravity may be associated with the pioneer habit in the wet forest

Adsorption geometry and electronic structure of iron phthalocyanine on Ag surfaces: A LEED and photoelectron momentum mapping study

We present a comprehensive study of the adsorption behavior of iron phthalocyanine on the low-index crystal faces of silver. By combining measurements of the reciprocal space by means of photoelectron momentum mapping and low energy electron diffraction, the real space adsorption geometries are reconstructed. At monolayer coverage ordered superstructures exist on all studied surfaces containing one molecule in the unit cell in case of Ag(100) and Ag(111), and two molecules per unit cell for Ag(110). The azimuthal tilt angle of the molecules against the high symmetry directions of the substrate is derived from the photoelectron momentum maps. A comparative analysis of the momentum patterns on the substrates with different symmetry indicates that both constituents of the twofold degenerate FePc lowest unoccupied molecular orbital are occupied by charge transfer from the substrate at the interface

An in vitro alveolar macrophage assay for predicting the short-term inhalation toxicity of nanomaterials

Additional file 1: Table S1. Comparison of significant in vitro LOAECs (significant as compared to the negative benchmark material corundum) to NOAECs and LOAECs recorded in rat STISs. Table S2. Bioactivity of four types of CeO2 NMs in rat STISs as compared to cellular effects recorded in the in vitro NR8383 AM assay

Temperature induced spin coherence dissipation in quantum dots

The temperature dependence of electron spin coherence in singly negatively charged (In,Ga)As/GaAs quantum dots is studied by time-resolved Faraday rotation. The decoherence time T2 is constant on a microsecond scale for temperatures below 20 K, for higher temperatures it shows a surprisingly sharp drop into the nanoseconds range. The decrease cannot be explained through inelastic scattering with phonons, and may be related with elastic scattering due to phonon-mediated fluctuations of the hyperfine interaction.Comment: 4 pages, 2 figure

Cellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G

Background: The activating immunoreceptor NKG2D is expressed on Natural Killer (NK) cells and subsets of T cells. NKG2D contributes to anti-tumour and anti-viral immune responses in vitro and in vivo. The ligands for NKG2D in humans are diverse proteins of the MIC and ULBP/RAET families that are upregulated on the surface of virally infected cells and tumours. Two splicing variants of ULBP5/RAET1G have been cloned previously, but not extensively characterised. Methodology/Principal Findings: We pursue a number of approaches to characterise the expression, trafficking, and function of the two isoforms of ULBP5/RAET1G. We show that both transcripts are frequently expressed in cell lines derived from epithelial cancers, and in primary breast cancers. The full-length transcript, RAET1G1, is predicted to encode a molecule with transmembrane and cytoplasmic domains that are unique amongst NKG2D ligands. Using specific anti-RAET1G1 antiserum to stain tissue microarrays we show that RAET1G1 expression is highly restricted in normal tissues. RAET1G1 was expressed at a low level in normal gastrointestinal epithelial cells in a similar pattern to MICA. Both RAET1G1 and MICA showed increased expression in the gut of patients with celiac disease. In contrast to healthy tissues the RAET1G1 antiserum stained a wide variety or different primary tumour sections. Both endogenously expressed and transfected RAET1G1 was mainly found inside the cell, with a minority of the protein reaching the cell surface. Conversely the truncated splicing variant of RAET1G2 was shown to encode a soluble molecule that could be secreted from cells. Secreted RAET1G2 was shown to downregulate NKG2D receptor expression on NK cells and hence may represent a novel tumour immune evasion strategy. Conclusions/Significance: We demonstrate that the expression patterns of ULBP5RAET1G are very similar to the well-characterised NKG2D ligand, MICA. However the two isoforms of ULBP5/RAET1G have very different cellular localisations that are likely to reflect unique functionality

Citizen OBservatory WEB (COBWEB): A Generic Infrastructure Platform to Facilitate the Collection of Citizen Science data for Environmental Monitoring

The mass uptake of internet connected, GPS enabled mobile devices has resulted in a surge of citizens active in making a huge variety of environmental observations.  The use and reuse potential of these data is significant but currently compromised by a lack of interoperability.  Useable standards either don’t exist, are neglected, poorly understood or tooling is unavailable.  Large volumes of data are being created but exist in silos.  This is a complex problem requiring sophisticated solutions balanced with the need to present sometimes unsophisticated users with comprehensible and useable software.  COBWEB has addressed this challenge by using the UNESCO World Network of Biosphere Reserves as a testbed for researching and developing a generic crowdsourcing infrastructure platform for environmental monitoring.   The solution arrived at provides tools for the creation of mobile Applications which generate data compliant with open interoperability standards and facilitate integration with Spatial Data Infrastructures.  COBWEB is a research project and the components of the COBWEB platform are at different Technology Readiness Levels. This paper outlines how the overall solution was arrived at, describes the main components developed and points to quality assurance, integration of sensors, interoperability and associated standardisation as key areas requiring further attention.

Mitochondrial DNA Regionalism and Historical Demography in the Extant Populations of Chirocephalus kerkyrensis (Branchiopoda: Anostraca)

Background: Mediterranean temporary water bodies are important reservoirs of biodiversity and host a unique assemblage of diapausing aquatic invertebrates. These environments are currently vanishing because of increasing human pressure. Chirocephalus kerkyrensis is a fairy shrimp typical of temporary water bodies in Mediterranean plain forests and has undergone a substantial decline in number of populations in recent years due to habitat loss. We assessed patterns of genetic connectivity and phylogeographic history in the seven extant populations of the species from Albania, Corfu Is. (Greece), Southern and Central Italy. Methodology/Principal Findings: We analyzed sequence variation at two mitochondrial DNA genes (Cytochrome Oxidase I and 16s rRNA) in all the known populations of C. kerkyrensis. We used multiple phylogenetic, phylogeographic and coalescence-based approaches to assess connectivity and historical demography across the whole distribution range of the species. C. kerkyrensis is genetically subdivided into three main mitochondrial lineages; two of them are geographically localized (Corfu Is. and Central Italy) and one encompasses a wide geographic area (Albania and Southern Italy). Most of the detected genetic variation (<81%) is apportioned among the aforementioned lineages. Conclusions/Significance: Multiple analyses of mismatch distributions consistently supported both past demographic and spatial expansions with the former predating the latter; demographic expansions were consistently placed during interglacial warm phases of the Pleistocene while spatial expansions were restricted to cold periods. Coalescence methods revealed a scenario of past isolation with low levels of gene flow in line with what is already known for other co-distributed fairy shrimps and suggest drift as the prevailing force in promoting local divergence. We recommend that these evolutionary trajectories should be taken in proper consideration in any effort aimed at protecting Mediterranean temporary water bodies

Adenylyl Cyclase Plays a Regulatory Role in Development, Stress Resistance and Secondary Metabolism in Fusarium fujikuroi

The ascomycete fungus Fusarium fujikuroi (Gibberella fujikuroi MP-C) produces secondary metabolites of biotechnological interest, such as gibberellins, bikaverin, and carotenoids. Production of these metabolites is regulated by nitrogen availability and, in a specific manner, by other environmental signals, such as light in the case of the carotenoid pathway. A complex regulatory network controlling these processes is recently emerging from the alterations of metabolite production found through the mutation of different regulatory genes. Here we show the effect of the targeted mutation of the acyA gene of F. fujikuroi, coding for adenylyl cyclase. Mutants lacking the catalytic domain of the AcyA protein showed different phenotypic alterations, including reduced growth, enhanced production of unidentified red pigments, reduced production of gibberellins and partially derepressed carotenoid biosynthesis in the dark. The phenotype differs in some aspects from that of similar mutants of the close relatives F. proliferatum and F. verticillioides: contrary to what was observed in these species, ΔacyA mutants of F. fujikuroi showed enhanced sensitivity to oxidative stress (H2O2), but no change in heavy metal resistance or in the ability to colonize tomato tissue, indicating a high versatility in the regulatory roles played by cAMP in this fungal group