Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications.

Based on recent results, the determination of the easily accessible red blood cell (RBC) membrane proteins may provide new diagnostic possibilities for assessing mutations, polymorphisms or regulatory alterations in diseases. However, the analysis of the current mass spectrometry-based proteomics datasets and other major databases indicates inconsistencies-the results show large scattering and only a limited overlap for the identified RBC membrane proteins. Here, we applied membrane-specific proteomics studies in human RBC, compared these results with the data in the literature, and generated a comprehensive and expandable database using all available data sources. The integrated web database now refers to proteomic, genetic and medical databases as well, and contains an unexpected large number of validated membrane proteins previously thought to be specific for other tissues and/or related to major human diseases. Since the determination of protein expression in RBC provides a method to indicate pathological alterations, our database should facilitate the development of RBC membrane biomarker platforms and provide a unique resource to aid related further research and diagnostics

Governing a shared hidden resource: A review of governance mechanisms for transboundary groundwater security

© 2017 Elsevier B.V. Globally, groundwater is by far the largest store of liquid freshwater, making it a key component of a secure water supply. However, over the past few decades the amount of usable groundwater available around the world has rapidly decreased. This depletion is caused primarily by mismanagement (e.g., overpumping, contamination, and under-regulation), but also by reduced natural recharge due to climate change and urbanization. Management of groundwater resources is particularly challenging for the nearly 600 aquifers that are transboundary, meaning that they extend across international political borders. To understand how governance mechanisms can reduce water insecurity in transboundary groundwater contexts, we review key literature from what we view as the most relevant fields: groundwater management, water security, international water law and international water governance. We then formulate a set of recommendations for improved groundwater governance that can address the specific physical nature of groundwater systems, enhance water security, and apply to transboundary groundwater settings. We argue that groundwater governance in transboundary contexts requires processes that (1) enhance context-specific and flexible international mechanisms; (2) address the perpetual need for groundwater data and information; (3) prioritize the precautionary principle and pollution prevention, in particular; (4) where appropriate, integrate governance of surface and subsurface water and land; and (5) expand institutional capacity, especially of binational or multinational actors

Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells

Vascular derivatives of human embryonic stem cells (hESC) are being developed as sources of tissue-specific cells for organ regeneration. However, identity of developmental pathways that modulate the specification of endothelial cells is not known yet. We studied phosphatidylinositol 3-kinase (PI3K)-Forkhead box O transcription factor 1A (FOXO1A) pathways during differentiation of hESC toward endothelial lineage and on proliferation, maturation, and cell death of hESC-derived endothelial cells (hESC-EC). During differentiation of hESC, expression of FOXO1A transcription factor was linked to the expression of a cluster of angiogenesis- and vascular remodeling-related genes. PI3K inhibitor LY294002 activated FOXO1A and induced formation of CD31(+) hESC-EC. In contrast, differentiating hESC with silenced FOXO1A by small interfering RNA (siRNA) showed lower mRNA levels of CD31 and angiopoietin2. LY294002 decreased proliferative activity of purified hESC-EC, while FOXO1A siRNA increased their proliferation. LY294002 inhibits migration and tube formation of hESC-EC; in contrast, FOXO1A siRNA increased in vitro tube formation activity of hESC-EC. After in vivo conditioning of cells in athymic nude rats, cells retain their low FOXO1A expression levels. PI3K/FOXO1A pathway is important for function and survival of hESC-EC and in the regulation of endothelial cell fate. Understanding these properties of hESC-EC may help in future applications for treatment of injured organs

Societal Adaptation to Decadal Climate Variability in the United States

The search for evidence of decadal climatic variability (DCV) has a very long history. In the past decade, a research community has coalesced around a series of roughly biennial workshops that have emphasized description of past DCV events; their causes and their teleconnections responsible for droughts, floods, and warm and cold spells around the world; and recently, the predictability of DCV events. Researchers studying climate change put great emphasis on prospective impacts, but the DCV community has yet to do so. To begin rectifying this deficiency, a short but ambitious workshop was convened in Waikoloa, near Kona, Hawaii, from 26-28 April 2007. This workshop, sponsored by the Center for Research on the Changing Earth System (CRCES), NOAA, the U.S. Geological Survey, and the U.S. Army Corps of Engineers, brought together climatologists and sectoral specialists representing agriculture, water resources, economics, the insurance industry, and developing country interests

A meta-analysis comparing the effectiveness of alternate day fasting, the 5:2 diet, and time-restricted eating for weight loss.

OBJECTIVE The objective of this meta-analysis was to compare the effectiveness of different intermittent fasting (IF) regimens on weight loss, in the general population, and compare these to traditional caloric energy restriction (CER). METHODS Three databases were searched from 2011 to June 2021 for randomized controlled trials (RCTs) that assessed weight loss and IF, including alternate day fasting (ADF), the 5:2 diet, and time-restricted eating (TRE). A random effect network analysis was used to compare the effectiveness between the three regimens. Meta-regression analysis was presented as weighted mean differences of body weight loss. RESULTS The exploratory random effects network analysis of 24 RCTs (n = 1768) ranked ADF as the most effective, followed by CER and TRE. The meta-analysis showed that IF regimens resulted in similar weight loss to CER (mean difference 0.26 kg, 95% CI: -0.31 to 0.84; p = 0.37). Compliance was generally high (>80%) in trials shorter than 3 months. CONCLUSIONS The present meta-analysis concludes that IF is comparable to CER and a promising alternative for weight loss. Among the three regimens, ADF showed the highest effectiveness for weight loss, followed by CER and TRE. Further well-powered RCTs with longer durations of intervention are required to draw solid conclusions

Dialogic science-policy networks for water security governance in the arid Americas

Addressing wicked problems challenging water security requires participation from multiple stakeholders, often with conflicting visions, complicating the attainment of water-security goals and heightening the need for integrative and effective science-policy interfaces. Sustained multi-stakeholder dialogues within science-policy networks can improve adaptive governance and water system resilience. This paper describes what we define as “dialogic science-policy networks,” or interactions -- both in structural and procedural terms -- between scientists and policy-makers that are: 1) interdisciplinary, 2) international (here, inter-American), 3) cross-sectoral, 4) open, 5) continual and iterative in the long-term, and 6) flexible. By fostering these types of interactions, dialogic networks achieve what we call the 4-I criteria for effective science-policy dialogues: inclusivity, involvement, interaction, and influence. Here we present several water-security research and action projects where some of these attributes may be present. Among these, a more comprehensive form of a dialogic network was intentionally created via AQUASEC, a virtual center and network initially fostered by a series of grants from the Inter-American Institute for Global Change Research. Subsequently, AQUASEC has significantly expanded to other regions through direct linkages and additional program support for the International Water Security Network, supported by Lloyd's Register Foundation and other sources. This paper highlights major scientific and policy achievements of a notable suite of science-policy networks, shared practices, methods, and knowledge integrating science and policy, as well as the main barriers overcome in network development. An important gap that remains for future research is the assessment and evaluation of dialogic science-policy networks' long-term outcomes.Fil: Lutz Ley, America N.. El Colegio de Sonora; MéxicoFil: Scott, Christopher A.. University of Arizona; Estados UnidosFil: Wilder, Margaret. University of Arizona; Estados UnidosFil: Varady, Robert G.. University of Arizona; Estados UnidosFil: Ocampo Melgar, Anahi. Universidad de Chile.; ChileFil: Lara Valencia, Francisco. Arizona State University; Estados UnidosFil: Zuniga Teran, Adriana. University of Arizona; Estados UnidosFil: Buechler, Stephanie. University of Arizona; Estados UnidosFil: Díaz Caravantes, Rolando. El Colegio de Sonora; MéxicoFil: Ribeiro Neto, Alfredo. El Colegio de Sonora; MéxicoFil: Pineda Pablos, Nicólas. El Colegio de Sonora; MéxicoFil: Martin, Facundo Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; Argentin

The decline and rise of neighbourhoods: the importance of neighbourhood governance

There is a substantial literature on the explanation of neighbourhood change. Most of this literature concentrates on identifying factors and developments behind processes of decline. This paper reviews the literature, focusing on the identification of patterns of neighbourhood change, and argues that the concept of neighbourhood governance is a missing link in attempts to explain these patterns. Including neighbourhood governance in the explanations of neighbourhood change and decline will produce better explanatory models and, finally, a better view about what is actually steering neighbourhood change