More than just a gut feeling : constraint-based genome-scale metabolic models for predicting functions of human intestinal microbes

The human gut is colonized with a myriad of microbes, with substantial interpersonal variation. This complex ecosystem is an integral part of the gastrointestinal tract and plays a major role in the maintenance of homeostasis. Its dysfunction has been correlated to a wide array of diseases, but the understanding of causal mechanisms is hampered by the limited amount of cultured microbes, poor understanding of phenotypes, and the limited knowledge about interspecies interactions. Genome-scale metabolic models (GEMs) have been used in many different fields, ranging from metabolic engineering to the prediction of interspecies interactions. We provide showcase examples for the application of GEMs for gut microbes and focus on (i) the prediction of minimal, synthetic, or defined media; (ii) the prediction of possible functions and phenotypes; and (iii) the prediction of interspecies interactions. All three applications are key in understanding the role of individual species in the gut ecosystem as well as the role of the microbiota as a whole. Using GEMs in the described fashions has led to designs of minimal growth media, an increased understanding of microbial phenotypes and their influence on the host immune system, and dietary interventions to improve human health. Ultimately, an increased understanding of the gut ecosystem will enable targeted interventions in gut microbial composition to restore homeostasis and appropriate host-microbe crosstalk.Peer reviewe

Bacteriological examination in place in five European countries to assess carcass fitness for consumption during meat inspection

In the European Union, bacteriological examination (BE) can be used as a decision support tool for an individual slaughter animal, if a clear decision regarding fitness for human consumption cannot be reached after performing the post-mortem meat inspection at the abattoir. The mandatory use of BE started already in the beginning of 20th century and the methods have since evolved in the different countries using it. Although still in use, discussions have taken place on whether BE is still a useful part of meat inspection. Currently, there is no European consensus regarding how to set up the methods or how to interpret the results. Still, there is a need to avoid unnecessary food waste, while at the same time guaranteeing food safety. In this descriptive study, we mapped the BE methods currently used in five European countries, namely Denmark, Finland, Germany, Italy and the Netherlands. The results show there is considerable variation between the countries regarding the specific analyses, sample matrices and media used. There is also variation in the indications when BE should be performed as well as when the results lead to condemnation. Although the results will be interpreted together with the pathological findings in the carcass, clearly written instructions should be available on how to interpret the results and when to perform condemnation. BE is used more often for cattle than for pigs, and e.g., in Denmark, BE is not used for pigs due to costs. Although BE can still be used to detect animals with a generalised infection at the time of slaughter, other methods that would be easier to standardise and accredit should be developed

Adult Lysophosphatidic Acid Receptor 1-Deficient Rats with Hyperoxia-Induced Neonatal Chronic Lung Disease Are Protected against Lipopolysaccharide-Induced Acute Lung Injury

Developmen

Global Networks of Trade and Bits

Considerable efforts have been made in recent years to produce detailed topologies of the Internet. Although Internet topology data have been brought to the attention of a wide and somewhat diverse audience of scholars, so far they have been overlooked by economists. In this paper, we suggest that such data could be effectively treated as a proxy to characterize the size of the "digital economy" at country level and outsourcing: thus, we analyse the topological structure of the network of trade in digital services (trade in bits) and compare it with that of the more traditional flow of manufactured goods across countries. To perform meaningful comparisons across networks with different characteristics, we define a stochastic benchmark for the number of connections among each country-pair, based on hypergeometric distribution. Original data are thus filtered by means of different thresholds, so that we only focus on the strongest links, i.e., statistically significant links. We find that trade in bits displays a sparser and less hierarchical network structure, which is more similar to trade in high-skill manufactured goods than total trade. Lastly, distance plays a more prominent role in shaping the network of international trade in physical goods than trade in digital services.Comment: 25 pages, 6 figure

Two shades of Green? The electorates of GreenLeft and the Party for the Animals

The Netherlands has two electorally significant parties that might be considered to be part of the Green' family: GreenLeft and the Party for the Animals. These two parties appeal to different niches of the Green electorate, identified on the basis of issue dimensions, demographics, and their trust in government. GreenLeft tends to attract voters from the traditional Green niche: those with egalitarian, cosmopolitan, environmentalist, and libertarian values. The Party for the Animals attracts another type of Green voter: significantly less cosmopolitan and evincing lower levels of political trust.</p