Translocation of Cyclin C During Oxidative Stress Is Regulated by Interactions with Multiple Trafficking Proteins

Eukaryotic cells take cues from their environment and interpret them to enact a response. External stresses can produce a decision between adjusting to behaviors which promote surviving the stress, or enacting a cell death program. The decision to undergo programmed cell death (PCD) is controlled by a complex interaction between nuclear and mitochondrial signals. The mitochondria are highly dynamic organelles that constantly undergo fission and fusion. However, a dramatic shift in mitochondrial morphology toward fission occurs early in the PCD process. We have identified the transcription factor cyclin C as the biochemical trigger for stress‐induced mitochondrial hyper‐fragmentation in yeast (Cooper et al., 2014 Dev. Cell) and mammalian (Wang et al., 2015, MCB) cells

Rapid South Atlantic spreading changes and coeval vertical motion in surrounding continents: Evidence for temporal changes of pressure-driven upper mantle flow

International audienceThe South Atlantic region displays (1) a topographic gradient across the basin, with Africa elevated relative to South America, (2) a bimodal spreading history with fast spreading rates in Late Cretaceous and Eo-Oligocene, and (3) episodic regional uplift events in the adjacent continents concentrated in Late Cretaceous and Oligocene. Here we show that these observations can be linked by dynamic processes within Earth's mantle, through temporal changes in asthenosphere flow beneath the region. The topographic gradient implies westward, pressure-driven mantle flow beneath the basin, while the rapid spreading rate changes, on order 10 million years, require significant decoupling of regional plate motion from the large-scale mantle buoyancy distribution through a mechanically weak asthenosphere. Andean topographic growth in late Miocene can explain the most recent South Atlantic spreading velocity reduction, arising from increased plate boundary forcing associated with the newly elevated topography. But this mechanism is unlikely to explain the Late Cretaceous/Tertiary spreading variations, as changes in Andean paleoelevation at the time are small. We propose an unsteady pressure-driven flow component in the asthenosphere beneath the South Atlantic region to explain the Late Cretaceous/Tertiary spreading rate variations. Temporal changes in mantle flow due to temporal changes in regional mantle pressure gradients imply a correlation of horizontal and vertical motions: we find that this prediction from our models agrees with geologic and geophysical observations of the South Atlantic region, including episodes of passive margin uplift, regional basin reactivation, and magmatic activity

Mechanisms of root reinforcement in soils:An experimental methodology using four-dimensional X-ray computed tomography and digital volume correlation

Vegetation on railway or highway slopes can improve slope stability through the generation of soil pore water suctions by plant transpiration and mechanical soil reinforcement by the roots. To incorporate the enhanced shearing resistance and stiffness of root-reinforced soils in stability calculations, it is necessary to understand and quantify its effectiveness. This requires integrated and sophisticated experimental and multiscale modelling approaches to develop an understanding of the processes at different length scales, from individual root-soil interaction through to full soil-profile or slope scale. One of the challenges with multiscale models is ensuring that they sufficiently closely represent real behaviour. This requires calibration against detailed high-quality and data-rich experiments. This study presents a novel experimental methodology, which combines in situ direct shear loading of a willow root reinforced soil with X-ray computed tomography to capture the 3D chronology of soil and root deformation within the shear zone. Digital volume correlation (DVC) analysis was applied to the computed tomography (CT) dataset to obtain full-field 3D displacement and strain information. This paper demonstrates the feasibility and discusses the challenges associated with DVC experiments on root-reinforced soils

Hillslope and stream connectivity: simulation of concentration-discharge patterns using the HYDRUS model

Abstract: Nutrient concentrations and loads in streamflow are sensitive to rapidly changing stream chemistry and discharge during storms. Mechanistic models that can simulate water and solute movement at hillslope scales could be useful for predicting concentration-discharge (C-Q) patterns and thereby improve our quantitative understanding of terrestrial-aquatic linkages for targeted catchment management. Our objective was to use the HYDRUS model to represent hydro-biogeochemical processes in soils that drive seepage of water and solutes from soil profiles into streams. Specifically we compared measurements in the literature with HYDRUS outputs using two methods for simulating runoff. This model predicts runoff (R) as rainfall that is instantaneously in excess of infiltration, but it is not designed to route runoff as overland flow. Post-HYDRUS addition of seepage to runoff was used to simulate the delivery of dissolved or particulate constituents to a stream (method A). Alternatively, we demonstrated how simulations using HYDRUS could include a hypothetical layer at the top of the soil profile with extremely high porosity and hydraulic conductivity that enabled overland flow and down-slope infiltration, but in this case only dissolved constituents could be considered (method B). These methods were evaluated by comparing the simulated temporal patterns of discharge and concentration with observed patterns. The catchments considered were in Slovenia (4210 ha) and in Australia (11.9 ha). Methods A and B were shown to adequately simulate some aspects of published discharge-concentration patterns, e.g. runoff dilution or concentration effects, but the temporal patterns of discharge for both methods did not precisely match those measured at small time-steps (e.g. 15 minutes). This limitation was due mainly to inadequate simulation of the down-slope movement of runoff and down-slope infiltration of a portion of this runoff. Method A was generally more useful than method B. Despite this limitation, both methods, if used carefully, should be adequate for many purposes, especially when simulating longer time-steps. Additional hypothetical simulations illustrated the significance of soil hydraulic conductivity, soil water content, and vertical gradients in solute concentrations in soil. Two temporal types of dischargeconcentration patterns were observed; short-term hysteresis caused by runoff during and shortly after a rainfall event, and longer-term trends associated with infiltration and seepage. Clockwise and anti-clockwise hysteresis was demonstrated to be potentially due to the temporal asynchrony of peak discharge and peak concentration in runoff. Simulations also demonstrated advantages over using the more common approach of a 2-or 3-component mixing model. Our results suggest that the HYDRUS model will be useful for the mechanistic simulation of within-soil processes that are needed to predict discharge-concentration patterns at hillslope scales

Growth and collapse of a deeply eroded orogen : insights from structural, geophysical, and geochronological constraints on the Pan-African evolution of NE Mozambique

This paper presents results of a large multidiciplinary geological mapping project in NE Mozambique, with a focus on the structural evolution of this part of the East African Orogen (EAO). It integrates field structural studies with geophysical interpretations and presents new geochronological data. The tectonic architecture of NE Mozambique can be subdivided into five megatectonic units on the basis of lithology, structure and geochronology: unit 1, Paleoproterozoic Ponta Messuli Complex in the extreme NW corner of NE Mozambique, which represents the local NW foreland to the EAO; unit 2, a collage of Mesoproterozoic metamorphic complexes, which forms the basement to unit 3, a stack of Neoproterozoic, NW directed imbricate thrust nappes named here the ‘‘Cabo Delgado Nappe Complex’’ (CDNC); unit 4, restricted Neoproterozoic metasedimentary basins; and unit 5, two exotic Neoproterozoic granulite me´ lange complexes. The units were assembled during a long and complex history of NWdirected shortening, which commenced with nappe stacking and emplacement of the CDNC over the Mesoproterozoic basement terranes toward the NW foreland. It is proposed that the CDNC and the Eastern Granulites farther north in Tanzania are remnants of Neoproterozoic volcanic arcs and microcontinents formed ‘‘outboard’’ of the Mesoproterozoic continent after 596 ± 11 Ma. Field and potential field geophysical data show that the nappes were folded by regional-scale NE–SW trending folds that formed in response to a later stage of the same shortening episode and this episode gave rise to the Lurio Belt, a prominent structural feature of northern Mozambique and a key element (often as suture zone) in many Gondwana reconstructions. The Lurio Belt is here interpreted as a structure generated during folding of the CDNC during later stages of the progressive shortening event. It is, however, a repeatedly reactivated shear zone, probably at the site of an older (Mesoproterozoic?) discontinuity, with an intense pure shear deformation history. It is cored by strongly attenuated lenses of a granulitic tectonic me´lange, the Ocua Complex (megatectonic unit 5) and is intruded by Late Pan-African granitoids of the Malema Suite. The compressional phase of the orogen was postdated by NW–SE directed extension. New U-Pb zircon and monazite dates show that extension was initiated at circa 540 Ma in the eastern Lurio Belt. It is argued that extension was the result of a major episode of orogenic collapse of the EAO, initiated by gravitational instabilities resulting from crustal thickening during the shortening phase

Evidence for non-merger co-evolution of galaxies and their supermassive black holes

Recent observational and theoretical studies have suggested that supermassive black holes (SMBHs) grow mostly through non-merger (`secular') processes. Since galaxy mergers lead to dynamical bulge growth, the only way to observationally isolate non-merger growth is to study galaxies with low bulge-to-total mass ratio (e.g. B/T < 10%). However, bulge growth can also occur due to secular processes, such as disk instabilities, making disk-dominated selections a somewhat incomplete way to select merger-free systems. Here we use the Horizon-AGN simulation to select simulated galaxies which have not undergone a merger since z = 2, regardless of bulge mass, and investigate their location on typical black hole-galaxy scaling relations in comparison to galaxies with merger dominated histories. While the existence of these correlations has long been interpreted as co-evolution of galaxies and their SMBHs driven by galaxy mergers, we show here that they persist even in the absence of mergers. We find that the correlations between SMBH mass and both total mass and stellar velocity dispersion are independent of B/T ratio for both merger-free and merger-dominated galaxies. In addition, the bulge mass and SMBH mass correlation is still apparent for merger-free galaxies, the intercept for which is dependent on B/T. Galaxy mergers reduce the scatter around the scaling relations, with merger-free systems showing broader scatter. We show that for merger-free galaxies, the co-evolution is dominated by radio-mode feedback, and suggest that the long periods of time between galaxy mergers make an important contribution to the co-evolution between galaxies and SMBHs in all galaxies.Comment: RJS and RSB are joint first authors. 12 pages, 7 figures, submitted to MNRA

Supermassive black holes in merger-free galaxies have higher spins which are preferentially aligned with their host galaxy

Here we use the Horizon-AGN simulation to test whether the spins of SMBHs in merger-free galaxies are higher. We select samples using an observationally motivated bulge-to-total mass ratio of < 0.1, along with two simulation motivated thresholds selecting galaxies which have not undergone a galaxy merger since z = 2, and those SMBHs with < 10% of their mass due to SMBH mergers. We find higher spins (> 5{\sigma} ) in all three samples compared to the rest of the population. In addition, we find that SMBHs with their growth dominated by BH mergers following galaxy mergers, are less likely to be aligned with their galaxy spin than those that have grown through accretion in the absence of galaxy mergers (3.4{\sigma} ). We discuss the implications this has for the impact of active galactic nuclei (AGN) feedback, finding that merger-free SMBHs spend on average 91% of their lifetimes since z = 2 in a radio mode of feedback (88% for merger-dominated galaxies). Given that previous observational and theoretical works have concluded that merger-free processes dominate SMBH-galaxy co-evolution, our results suggest that this co-evolution could be regulated by radio mode AGN feedback.Comment: RSB and RJS are joint first authors. Submitted to MNRAS, 10 page

Black-wattle growth in reponse to application of nitrogen, phosphorus and potassium

Due to the lack of information about Black-wattle fertilization, this study evaluated black-wattle plants growth in response to different fertilization levels of nitrogen, phosphorus and potassium six years after implantation. The statistical design used was a randomized blocks with trifatorial distribution. Total height (m), diameter at breast height (DBH) (cm) and stem volume with bark (m³ ha-1) were evaluated. Black-wattle showed a positive and significant growth response to N and P (interaction) fertilizations and absence for K. m To obtain the maximum development of black-wattle, for the soil and climate condition studied, it is required the use of the maximum dose of nitrogen (40.0 kg ha-1 N) and 78.9 kg ha-1 phosphorus, not requiring the addition of potassium

Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal.

The high mortality of melanoma is caused by rapid spread of cancer cells, which occurs unusually early in tumour evolution. Unlike most solid tumours, thickness rather than cytological markers or differentiation is the best guide to metastatic potential. Multiple stimuli that drive melanoma cell migration have been described, but it is not clear which are responsible for invasion, nor if chemotactic gradients exist in real tumours. In a chamber-based assay for melanoma dispersal, we find that cells migrate efficiently away from one another, even in initially homogeneous medium. This dispersal is driven by positive chemotaxis rather than chemorepulsion or contact inhibition. The principal chemoattractant, unexpectedly active across all tumour stages, is the lipid agonist lysophosphatidic acid (LPA) acting through the LPA receptor LPAR1. LPA induces chemotaxis of remarkable accuracy, and is both necessary and sufficient for chemotaxis and invasion in 2-D and 3-D assays. Growth factors, often described as tumour attractants, cause negligible chemotaxis themselves, but potentiate chemotaxis to LPA. Cells rapidly break down LPA present at substantial levels in culture medium and normal skin to generate outward-facing gradients. We measure LPA gradients across the margins of melanomas in vivo, confirming the physiological importance of our results. We conclude that LPA chemotaxis provides a strong drive for melanoma cells to invade outwards. Cells create their own gradients by acting as a sink, breaking down locally present LPA, and thus forming a gradient that is low in the tumour and high in the surrounding areas. The key step is not acquisition of sensitivity to the chemoattractant, but rather the tumour growing to break down enough LPA to form a gradient. Thus the stimulus that drives cell dispersal is not the presence of LPA itself, but the self-generated, outward-directed gradient