Phase diagram of orbital-selective Mott transitions at finite temperatures

Mott transitions in the two-orbital Hubbard model with different bandwidths are investigated at finite temperatures. By means of the self-energy functional approach, we discuss the stability of the intermediate phase with one orbital localized and the other itinerant, which is caused by the orbital-selective Mott transition (OSMT). It is shown that the OSMT realizes two different coexistence regions at finite temperatures in accordance with the recent results of Liebsch. We further find that the particularly interesting behavior emerges around the special condition $U=U'$ and J=0, which includes a new type of the coexistence region with three distinct states. By systematically changing the Hund coupling, we establish the global phase diagram to elucidate the key role played by the Hund coupling on the Mott transitions.Comment: 4 pages, 6 figure

Crystal structure of the catalytic fragment of murine poly(ADP-ribose) polymerase-2.

Poly(ADP-ribose) polymerase-1 (PARP-1) has become an important pharmacological target in the treatment of cancer due to its cellular role as a 'DNA-strand break sensor', which leads in part to resistance to some existing chemo- and radiological treatments. Inhibitors have now been developed which prevent PARP-1 from synthesizing poly(ADP-ribose) in response to DNA-breaks and potentiate the cytotoxicity of DNA damaging agents. However, with the recent discoveries of PARP-2, which has a similar DNA-damage dependent catalytic activity, and additional members containing the 'PARP catalytic' signature, the isoform selectivity and resultant pharmacological effects of existing inhibitors are brought into question. We present here the crystal structure of the catalytic fragment of murine PARP-2, at 2.8 A resolution, and compare this to the catalytic fragment of PARP-1, with an emphasis on providing a possible framework for rational drug design in order to develop future isoform-specific inhibitors

Flight path analysis in sounding rocket “Libertador I” with computational simulation on three degrees of freedom

This paper presents obtained results from computer simulations in order to analyze the flight path of the sounding rocket Libertador I, developed in 2012 at the Fundación Universitaria Los Libertadores. The main aspects that affect the motion of a rocket are propulsion and aerodynamics effects. Numerical algorithms were implemented to value the thrust provided by the rocket motor as well as aerodynamic loads. Then, the differential equations of the motion are solved using the Runge-Kutta method, RK9. Trajectory and other numerical results are calculated from a code developed in FORTRAN. Finally, graphs of the flight path, curves of the vehicle velocity as function of time are presented. Also, the algorithm permits to determine the maximum altitude and possible impact sites.Facultad de Ingenierí

Flight path analysis in sounding rocket “Libertador I” with computational simulation on three degrees of freedom

This paper presents obtained results from computer simulations in order to analyze the flight path of the sounding rocket Libertador I, developed in 2012 at the Fundación Universitaria Los Libertadores. The main aspects that affect the motion of a rocket are propulsion and aerodynamics effects. Numerical algorithms were implemented to value the thrust provided by the rocket motor as well as aerodynamic loads. Then, the differential equations of the motion are solved using the Runge-Kutta method, RK9. Trajectory and other numerical results are calculated from a code developed in FORTRAN. Finally, graphs of the flight path, curves of the vehicle velocity as function of time are presented. Also, the algorithm permits to determine the maximum altitude and possible impact sites.Facultad de Ingenierí

Las acciones de ejecución y de prohibición en Entre Ríos

El autor  considera a las acciones de ejecución y de prohibición como instituciones  procesales no suficientemente analizadas en el ámbito porteño. Sin embargo,  tienen larga tradición en  algunas constituciones provinciales y fueron receptadas de los “writs” del Common Law; muestran, asimismo,  marcadas semejanzas con el trámite procesal del  amparo, ya que se desprendieron del habeas corpus como garantía libertaria “madre”, pero también  tienen diferencias en cuanto a su naturaleza jurídica que,  tardíamente, reconoció la ley nº 8369/90. Se describen la genealogía de estos institutos, su recepción constitucional, su reglamentación legislativa, su íter procesal y su aplicación judicial. Se analiza, finalmente, a los institutos, desde una perspectiva histórica, fáctica, normológica y axiológica a fin de proponer su reglamentación a nivel nacional como un modo concreto de control de la actividad administrativa reglada, y así potenciar los controles de las instituciones republicanas

Large-scale Production And Purification Of Recombinant Protein From An Insect Cell/baculovirus System In Erlenmeyer Flasks: Application To The Chicken Poly(adp-ribose) Polymerase Catalytic Domain

A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 × 109 infected cells in three liters of culture.308923928Althaus, F.R., Richter, C., ADP-ribosylation of proteins. Enzymology and biological significance (1987) Molecular Biology, Biochemistry and Biophysics, 37, pp. 1-126Lautier, D., Lagueaux, J., Ménard, L., Poirier, G.C., Molecular and biochemical features of poly(ADP-ribose) metabolism (1993) Molecular and Cellular Biochemistry, 122, pp. 171-193Satoh, M.S., Lindahl, T., Role of poly(ADP-ribose) formation in DNA repair (1992) Nature, 356, pp. 356-358Molinete, M., Vermeulen, W., Bürkle, A., Ménissier-de Murcia, J., Küpper, J., Hoeijmakers, J., De Murcia, G., Overproduction of the poly(ADP-ribose) polymerase DNA-binding domain blocks alkylation-induced DNA repair synthesis in mammalian cells (1993) EMBO Journal, 5, pp. 2109-2117Masson, M., Rolli, V., Dantzer, F., Trucco, C., Schrieber, V., Fribourg, S., Molinete, M., De Murcia, G., Poly(ADP-ribose) polymerase: Structure-function relationship (1995) Biochimie, 77, pp. 456-461Suto, M.L., Suto, M.S., Inhibitors of poly(ADP-ribose) polymerase (ADPRP): Potential chemotherapeutic agents (1991) Drugs of the Future, 16, pp. 723-739Giner, H., Simonin, F., De Murcia, G., Ménissier-de Murcia, J., Overproduction and large-scale purification of the human poly(ADP-ribose) polymerase using a baculovirus expression system (1992) Gene, 114, pp. 279-283Simonin, F., Höfferer, L., Panzeter, P., Muller, S., De Murcia, G., Althaus, F.R., The carboxy-terminal domain of human poly(ADP-ribose) polymerase: Overproduction in Escherichia coli, large scale purification and characterization (1993) Journal of Molecular Biology, 268, pp. 13454-13461Murhammer, D.W., Review and patents literature. The use of insect cell cultures for recombinant protein synthesis: Engineering aspects (1991) Applied Biochemistry and Biotechnology, 31, pp. 283-310Goosen, M.F.A., Large-scale insect cell culture (1992) Current Opinion in Biotechnology, 3, pp. 99-104Kamen, A.A., Tom, R.L., Caron, A.W., Chavarie, C., Massie, B., Archambault, J., Culture of insect cells in a helical ribbon impeller bioreactor (1991) Biotechnology and Bioengineering, 38, pp. 619-628Van Lier, F.L.J., Van Den End, E.J., Gooijer, C.D., Vlak, J.M., Tramper, J., Continuous production of baculovirus in a cascade insect-cell reactor (1990) Applied Microbiology and Biotechnology, 33, pp. 43-47Power, J., Greenfield, P.F., Nielsen, L., Reid, S., Modelling the growth and protein production by insect cells following infection by a recombinant baculovirus in suspension culture (1992) Cytotechnology, 9, pp. 149-155Stavroulakis, D.A., Kalogerakis, N., Behie, L.A., Latrou, K., Kinetic data for the BM-5 insect line in repeated-batch suspension culture (1991) Biotechnology, 38, pp. 116-126Neutra, R., Levi, B.-Z., Shoham, Y., Optimization of protein-production by the baculovirus expression vector system in shake flasks (1992) Applied Microbiology and Biotechnology, 37, pp. 74-78Luckow, V.A., Summers, M.D., Signals important for high level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors (1989) Virology, 170, pp. 31-39O'Reily, D.R., Miller, L.K., Luckow, V.A., (1992) Baculovirus Expression System: A Laboratory Manual, , Freeman, New YorkScott, R.L., Blanchard, J.H., Fergusson, C.H.R., Effects of oxygen on recombinant protein production by suspension cultures of Spodoptera frugiperda Sf9 insect cell (1992) Enzyme and Microbial Technology, 14, pp. 798-80