Large-scale Production And Purification Of Recombinant Protein From An Insect Cell/baculovirus System In Erlenmeyer Flasks: Application To The Chicken Poly(adp-ribose) Polymerase Catalytic Domain
A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 × 109 infected cells in three liters of culture.308923928Althaus, F.R., Richter, C., ADP-ribosylation of proteins. Enzymology and biological significance (1987) Molecular Biology, Biochemistry and Biophysics, 37, pp. 1-126Lautier, D., Lagueaux, J., Ménard, L., Poirier, G.C., Molecular and biochemical features of poly(ADP-ribose) metabolism (1993) Molecular and Cellular Biochemistry, 122, pp. 171-193Satoh, M.S., Lindahl, T., Role of poly(ADP-ribose) formation in DNA repair (1992) Nature, 356, pp. 356-358Molinete, M., Vermeulen, W., Bürkle, A., Ménissier-de Murcia, J., Küpper, J., Hoeijmakers, J., De Murcia, G., Overproduction of the poly(ADP-ribose) polymerase DNA-binding domain blocks alkylation-induced DNA repair synthesis in mammalian cells (1993) EMBO Journal, 5, pp. 2109-2117Masson, M., Rolli, V., Dantzer, F., Trucco, C., Schrieber, V., Fribourg, S., Molinete, M., De Murcia, G., Poly(ADP-ribose) polymerase: Structure-function relationship (1995) Biochimie, 77, pp. 456-461Suto, M.L., Suto, M.S., Inhibitors of poly(ADP-ribose) polymerase (ADPRP): Potential chemotherapeutic agents (1991) Drugs of the Future, 16, pp. 723-739Giner, H., Simonin, F., De Murcia, G., Ménissier-de Murcia, J., Overproduction and large-scale purification of the human poly(ADP-ribose) polymerase using a baculovirus expression system (1992) Gene, 114, pp. 279-283Simonin, F., Höfferer, L., Panzeter, P., Muller, S., De Murcia, G., Althaus, F.R., The carboxy-terminal domain of human poly(ADP-ribose) polymerase: Overproduction in Escherichia coli, large scale purification and characterization (1993) Journal of Molecular Biology, 268, pp. 13454-13461Murhammer, D.W., Review and patents literature. The use of insect cell cultures for recombinant protein synthesis: Engineering aspects (1991) Applied Biochemistry and Biotechnology, 31, pp. 283-310Goosen, M.F.A., Large-scale insect cell culture (1992) Current Opinion in Biotechnology, 3, pp. 99-104Kamen, A.A., Tom, R.L., Caron, A.W., Chavarie, C., Massie, B., Archambault, J., Culture of insect cells in a helical ribbon impeller bioreactor (1991) Biotechnology and Bioengineering, 38, pp. 619-628Van Lier, F.L.J., Van Den End, E.J., Gooijer, C.D., Vlak, J.M., Tramper, J., Continuous production of baculovirus in a cascade insect-cell reactor (1990) Applied Microbiology and Biotechnology, 33, pp. 43-47Power, J., Greenfield, P.F., Nielsen, L., Reid, S., Modelling the growth and protein production by insect cells following infection by a recombinant baculovirus in suspension culture (1992) Cytotechnology, 9, pp. 149-155Stavroulakis, D.A., Kalogerakis, N., Behie, L.A., Latrou, K., Kinetic data for the BM-5 insect line in repeated-batch suspension culture (1991) Biotechnology, 38, pp. 116-126Neutra, R., Levi, B.-Z., Shoham, Y., Optimization of protein-production by the baculovirus expression vector system in shake flasks (1992) Applied Microbiology and Biotechnology, 37, pp. 74-78Luckow, V.A., Summers, M.D., Signals important for high level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors (1989) Virology, 170, pp. 31-39O'Reily, D.R., Miller, L.K., Luckow, V.A., (1992) Baculovirus Expression System: A Laboratory Manual, , Freeman, New YorkScott, R.L., Blanchard, J.H., Fergusson, C.H.R., Effects of oxygen on recombinant protein production by suspension cultures of Spodoptera frugiperda Sf9 insect cell (1992) Enzyme and Microbial Technology, 14, pp. 798-80
