Military supply, everyday demand, and reindeer : Zooarchaeology of Nazi German Second World War military presence in Finnish Lapland, Northernmost Europe

During the Second World War, in 1941-1944, Nazi German troops held the frontal responsibility of the Arctic front in Finnish Lapland. In this paper, we present the first zooarchaeological study of the wartime faunal remains from German military camps in Lapland. This illustrates the supply situation of both the German soldiers and their multinational prisoners. The official military supply was substantially supplemented with local food sources, namely, with the local semi-domesticated reindeer that dominates the bone assemblage. Bones of cattle, ovicaprines, and pig occur in lower numbers and appear to represent the German long-distance supply chain stretching from the Mediterranean to the Arctic Ocean. The remains of reindeer and wild species remind of the close interactions with locals and of the prisoners' hunting activities to supplement their meager diet. Even if the reindeer bones dominate both the soldiers' and prisoners' faunal assemblages, there are notable differences in the body parts, with bones from meatier portions always found in the soldiers' food waste. Besides highlighting a tension between the military supply and everyday demands, the faunal remains can draw attention to wider anthropological questions that reach beyond the information available in historical documents, such as adaptations into an alien northern environment. This emphasizes the importance of zooarchaeological analyses of recent past faunal materials from superficially familiar contexts.Peer reviewe

Tetradecylthioacetic acid inhibits proliferation of human SW620 colon cancer cells - gene expression profiling implies endoplasmic reticulum stress

Background: Previous reports have shown an antiproliferative effect of the synthetic, 3-thia fatty acid tetradecylthioacetic acid (TTA) on different cancer cells in vitro and in vivo. The mechanisms behind the observed effects are poorly understood. We therefore wanted to explore the molecular mechanisms involved in TTA-induced growth inhibition of the human colon cancer cell line SW620 by gene expression profiling. Methods: An antiproliferative effect of TTA on SW620 cells in vitro was displayed in real time using the xCELLigence System (Roche). Affymetrix gene expression profiling was performed to elucidate the molecular mechanisms behind the antiproliferative effect of TTA. Changes in gene expression were verified at protein level by western blotting. Results: TTA reduced SW620 cell growth, measured as baseline cell index, by 35% and 55% after 48 h and 72 h, respectively. We show for the first time that TTA induces an endoplasmic reticulum (ER) stress response in cancer cells. Gene expression analysis revealed changes related to ER stress and unfolded protein response (UPR). This was verified at protein level by phosphorylation of eukaryote translation initiation factor 2 alpha (eIF2a) and downstream upregulation of activating transcription factor 4 (ATF4). Transcripts for positive and negative cell cycle regulators were down- and up-regulated, respectively. This, together with a down-regulation of Cyclin D1 at protein level, indicates inhibition of cell cycle progression. TTA also affected transcripts involved in calcium homeostasis. Moreover, mRNA and protein level of the ER stress inducible C/EBP-homologous protein (CHOP), Tribbles homolog 3 (Drosophila) (TRIB3) and CCAAT/enhancer binding protein beta (C/EBPb) were enhanced, and the C/EBPb LIP/LAP ratio was significantly increased. These results indicate prolonged ER stress and a possible link to induction of cell death. Conclusion: We find that TTA-induced growth inhibition of SW620 cells seems to be mediated through induction of ER stress and activation of the UPR pathway

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2b-RAD sequence reads. 30 files (1 per population) because of file size limitation