Detection of vanA-containing Enterococcus species in faecal microbiota of gilthead seabream (Sparus aurata)

Vancomycin-resistant Enterococcus faecalis, E. faecium and E. durans isolates with the genotype vanA were detected in 7 of 118 faecal samples (5.9%) of natural gilthead seabream recovered off the coast of Portugal, and one vancomycinresistant isolate/sample was further characterized. The genes erm(B), tet(L), tet(M), aac(6')-aph(2"), aph(3')-IIIa and/ or ant(6)-Ia were identified in most of the 7 vancomycin-resistant enterococci. Sequence types ST273, ST313 and ST76 were detected in three E. faecium isolates and ST6 in two E. faecalis isolates. VanA-containing enterococci are suggested to be disseminated in fish in marine ecosystems close to areas of human activity

Staphylococcus aureus and Methicillin-resistant coagulase-negative staphylococci in nostrils and buccal mucosa of healthy camels used for recreational purposes

Several different species of animals host staphylococci as normal microbiota. These animals can be a source of staphylococci zoonotic infections. People with routine or occupational exposure to infected/colonized animals are at risk of a potential transmission. Therefore, we aimed to investigate the presence of S. aureus and other staphylococci in camels used for recreational purposes as well as their antimicrobial resistance, virulence factors and genetic lineages. A total of 172 samples were collected from 86 healthy camels (nose and mouth) from different farms located in the Canary Islands, Spain. Antimicrobial susceptibility testing was performed against 14 antimicrobial agents. The presence of virulence genes was studied by PCR. Multilocus sequence typing, spa typing and agr typing were performed in all S. aureus isolates. From the 86 camels tested, 42 staphylococci were isolated, of which there were 11 S. aureus, 13 S. lentus, 12 S. sciuri, 3 S. xylosus, S. epidermidis, S. hominis and S. chromogenes. Staphylococci isolates were resistant to penicillin, ciprofloxacin, clindamycin and fusidic acid. All S. aureus isolates harbored the hla, hlb and hld virulence genes. S. aureus isolates were ascribed to three sequence types (STs) and three spa types. All S. aureus isolates belonged to agr type III. Camels from Gran Canaria used in recreational purposes have a moderate prevalence of S. aureus and other coagulase-negative staphylococci. Nevertheless, S. aureus isolates are susceptible to almost all antibiotics tested.Na publicação: Newton Verbisck

Ultrasonic energy for proteomics: when, where and how

Comunicaciones a congreso

Polymorphism of granule-bound starch synthase proteins of bread wheat (Triticum aestivum L.) cultivated in Portugal

International audienc

Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.)

absen

Detection and genetic characterisation of vanA-containing enterococcus strains in healthy lusitano horses

Lusitano horses were investigated in order to detect the presence of vancomycin-resistant enterococci. vanA isolates showed high level vancomycin (Minimum inhibitory concentration; MIC 128 mg/l) and teicoplanin resistance (MIC 64 mg/l), as well as resistance to ciprofloxacin, erythromycin and tetracycline. The tet(L) and erm(B) genes, associated with tetracycline and erythromycin resistance, respectively, were found in all vanA isolates.The intestinal tract of Lusitano horses can be a potential reservoir for vanA-containing enterococci. © 2009 EVJ Ltd

Genetical, biochemical and technological parameters associated with biscuit quality. II. Prediction using storage proteins and quality characteristics in a soft wheat population

DOI:10.1006/jcrs.2002.0452absen

Genetic analysis of the size of endosperm starch granules in a mapped segregating wheat population

DOI:10.1006/jcrs.2001.0422absen

Mapping QTLs for grain hardness and puroindoline content in wheat (Triticum aestivum)

DOI:10.1007/s00122-002-0971-8absen